Subtiligase: A Tool for Semisynthesis of Proteins

A variant of subtilisin BPN', which we call subtiligase, has been used to ligate esterified peptides site-specifically onto the N termini of proteins or peptides in aqueous solution and in high yield. We have produced biotinylated or heavy-atom derivatives of methionyl-extended human growth hor...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1994-12, Vol.91 (26), p.12544-12548
Hauptverfasser:	Chang, Thomas K., Jackson, David Y., Burnier, John P., Wells, James A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino acids Bacteriophages Base Sequence Biochemistry DNA Enzymes Esters Hydrochlorides In Vitro Techniques Ligation Molecular Sequence Data Peptides - chemical synthesis Peptides - chemistry Proteins Recombinant Proteins Structure-Activity Relationship Substrate Specificity Subtilisins - chemistry Subtilisins - metabolism
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