Real-Time RNA Profiling within a Single Bacterium

Characterizing the dynamics of specific RNA levels requires real-time RNA profiling in a single cell. We show that the combination of a synthetic modular genetic system with fluorescence correlation spectroscopy allows us to directly measure in real time the activity of any specific promoter in prok...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2005-06, Vol.102 (26), p.9160-9164
Hauptverfasser:	Le, Thuc T., Harlepp, Sébastien, Guet, Cǎlin C., Dittmar, Kimberly, Emonet, Thierry, Pan, Tao, Cluzel, Philippe, Haselkorn, Robert
Format:	Artikel
Sprache:	eng
Schlagworte:	Autocorrelation B lymphocytes Bacteria Bacterial Proteins - chemistry Biological Sciences Cell Cycle Cell Proliferation Cells Dose-Response Relationship, Drug Drug Resistance, Multiple Escherichia coli - metabolism Escherichia coli Proteins - chemistry Fluorescence Gene Expression Regulation, Bacterial Genes, Reporter Genotype Green Fluorescent Proteins - metabolism Mathematical functions Models, Statistical Molecules Plasmids Plasmids - metabolism Prokaryotic cells Promoter Regions, Genetic Protein Binding Real time Recombinant Fusion Proteins - chemistry Ribonucleic acid RNA RNA - chemistry RNA - metabolism RNA, Messenger - metabolism Spectrometry, Fluorescence Spliceosomes - metabolism Time Factors Transcription, Genetic
Online-Zugang:	Volltext
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