Leptin and Leptin Receptor mRNA and Protein Expression in the Murine Fetus and Placenta
Leptin is a 167-aa protein that is secreted from adipose tissue and is important in the regulation of energy balance. It also functions in hematopoiesis and reproduction. To assess whether leptin is involved in fetal growth and development we have examined the distribution of mRNAs encoding leptin a...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1997-09, Vol.94 (20), p.11073-11078 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Hoggard, Nigel Hunter, Leif Duncan, Jacqueline S. Williams, Lynda M. Trayhurn, Paul Mercer, Julian G. |
| description | Leptin is a 167-aa protein that is secreted from adipose tissue and is important in the regulation of energy balance. It also functions in hematopoiesis and reproduction. To assess whether leptin is involved in fetal growth and development we have examined the distribution of mRNAs encoding leptin and the leptin receptor (which has at least six splice variants) in the 14.5-day postcoitus mouse fetus and in the placenta using reverse transcription-PCR and in situ hybridization. High levels of gene expression for leptin, the leptin receptor, and the long splice variant of the leptin receptor with an intracellular signaling domain were observed in the placenta, fetal cartilage/bone, and hair follicles. Receptor expression also was detected in the lung, as well as the leptomeninges and choroid plexus of the fetal brain. Western blotting and immunocytochemistry, using specific antibodies, demonstrated the presence of leptin and leptin receptor protein in these tissues. These results suggest that leptin may play a role in the growth and development of the fetus, both through placental and fetal expression of the leptin and leptin receptor genes. In the fetus, leptin may be multifunctional and have both paracrine and endocrine effects. |
| doi_str_mv | 10.1073/pnas.94.20.11073 |
| format | Article |
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It also functions in hematopoiesis and reproduction. To assess whether leptin is involved in fetal growth and development we have examined the distribution of mRNAs encoding leptin and the leptin receptor (which has at least six splice variants) in the 14.5-day postcoitus mouse fetus and in the placenta using reverse transcription-PCR and in situ hybridization. High levels of gene expression for leptin, the leptin receptor, and the long splice variant of the leptin receptor with an intracellular signaling domain were observed in the placenta, fetal cartilage/bone, and hair follicles. Receptor expression also was detected in the lung, as well as the leptomeninges and choroid plexus of the fetal brain. Western blotting and immunocytochemistry, using specific antibodies, demonstrated the presence of leptin and leptin receptor protein in these tissues. These results suggest that leptin may play a role in the growth and development of the fetus, both through placental and fetal expression of the leptin and leptin receptor genes. In the fetus, leptin may be multifunctional and have both paracrine and endocrine effects.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.94.20.11073</identifier><identifier>PMID: 9380761</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Antiserum ; Biological Sciences ; Blotting, Western ; Carrier Proteins - genetics ; Carrier Proteins - metabolism ; Complementary DNA ; Female ; Fetus ; Fetus - metabolism ; Fetuses ; Genetics ; Immunohistochemistry ; In Situ Hybridization ; Leptin ; Lungs ; Medical research ; Messenger RNA ; Mice ; Placenta ; Placenta - metabolism ; Polymerase Chain Reaction ; Pregnancy ; Proteins - genetics ; Proteins - metabolism ; Receptors ; Receptors, Cell Surface ; Receptors, Leptin ; Reverse transcriptase polymerase chain reaction ; Ribonucleic acid ; RNA ; RNA Splicing ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Rodents</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1997-09, Vol.94 (20), p.11073-11078</ispartof><rights>Copyright 1993-1997 National Academy of Sciences</rights><rights>Copyright National Academy of Sciences Sep 30, 1997</rights><rights>Copyright © 1997, The National Academy of Sciences of the USA 1997</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c589t-883eb6ed8dc714f87a613b104b0585c2714e5f73957225818f7c48d43894a9c13</citedby><cites>FETCH-LOGICAL-c589t-883eb6ed8dc714f87a613b104b0585c2714e5f73957225818f7c48d43894a9c13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/94/20.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/43466$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/43466$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9380761$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hoggard, Nigel</creatorcontrib><creatorcontrib>Hunter, Leif</creatorcontrib><creatorcontrib>Duncan, Jacqueline S.</creatorcontrib><creatorcontrib>Williams, Lynda M.</creatorcontrib><creatorcontrib>Trayhurn, Paul</creatorcontrib><creatorcontrib>Mercer, Julian G.</creatorcontrib><title>Leptin and Leptin Receptor mRNA and Protein Expression in the Murine Fetus and Placenta</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Leptin is a 167-aa protein that is secreted from adipose tissue and is important in the regulation of energy balance. 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These results suggest that leptin may play a role in the growth and development of the fetus, both through placental and fetal expression of the leptin and leptin receptor genes. In the fetus, leptin may be multifunctional and have both paracrine and endocrine effects.</description><subject>Animals</subject><subject>Antiserum</subject><subject>Biological Sciences</subject><subject>Blotting, Western</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - metabolism</subject><subject>Complementary DNA</subject><subject>Female</subject><subject>Fetus</subject><subject>Fetus - metabolism</subject><subject>Fetuses</subject><subject>Genetics</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization</subject><subject>Leptin</subject><subject>Lungs</subject><subject>Medical research</subject><subject>Messenger RNA</subject><subject>Mice</subject><subject>Placenta</subject><subject>Placenta - metabolism</subject><subject>Polymerase Chain Reaction</subject><subject>Pregnancy</subject><subject>Proteins - genetics</subject><subject>Proteins - metabolism</subject><subject>Receptors</subject><subject>Receptors, Cell Surface</subject><subject>Receptors, Leptin</subject><subject>Reverse transcriptase polymerase chain reaction</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA Splicing</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Rodents</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks1rFDEYxoModa3eRRAHD9LLrPmcJOCllNYKqy1F8RiyM-_YWWaTaZKR9r9vpjss1oOe8vH8nvdN8gSh1wQvCZbs4-BsXGq-pHk9bTxBC4I1KSuu8VO0wJjKUnHKn6MXMW4wxloofIAONFNYVmSBfq5gSJ0rrGuKeXoFdZ74UGyvvh0_CJfBJ8jK6e0QIMbOuyKv0jUUX8fQOSjOII1xh_a2BpfsS_SstX2EV_N4iH6cnX4_OS9XF5-_nByvyloonUqlGKwraFRTS8JbJW1F2JpgvsZCiZrmTRCtZFpISoUiqpU1Vw1nSnOra8IO0add3WFcb6GZegfbmyF0WxvujLedeay47tr88r8NZRVW2f5htgd_M0JMZtvFGvreOvBjNDI_FKNC_hckFWWcMJ3B93-BGz8Gl9_AUEyYoEpNbfEOqoOPMUC7PzDBZsrRTMEazbPHPASbLW__vOjeMCeZ9XezPjn36qMKR_8mTDv2fYLblNE3O3QT80fYs5zxqmL3rsy_Jg</recordid><startdate>19970930</startdate><enddate>19970930</enddate><creator>Hoggard, Nigel</creator><creator>Hunter, Leif</creator><creator>Duncan, Jacqueline S.</creator><creator>Williams, Lynda M.</creator><creator>Trayhurn, Paul</creator><creator>Mercer, Julian G.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><general>The National Academy of Sciences of the USA</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19970930</creationdate><title>Leptin and Leptin Receptor mRNA and Protein Expression in the Murine Fetus and Placenta</title><author>Hoggard, Nigel ; Hunter, Leif ; Duncan, Jacqueline S. ; Williams, Lynda M. ; Trayhurn, Paul ; Mercer, Julian G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c589t-883eb6ed8dc714f87a613b104b0585c2714e5f73957225818f7c48d43894a9c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Antiserum</topic><topic>Biological Sciences</topic><topic>Blotting, Western</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - metabolism</topic><topic>Complementary DNA</topic><topic>Female</topic><topic>Fetus</topic><topic>Fetus - metabolism</topic><topic>Fetuses</topic><topic>Genetics</topic><topic>Immunohistochemistry</topic><topic>In Situ Hybridization</topic><topic>Leptin</topic><topic>Lungs</topic><topic>Medical research</topic><topic>Messenger RNA</topic><topic>Mice</topic><topic>Placenta</topic><topic>Placenta - metabolism</topic><topic>Polymerase Chain Reaction</topic><topic>Pregnancy</topic><topic>Proteins - genetics</topic><topic>Proteins - metabolism</topic><topic>Receptors</topic><topic>Receptors, Cell Surface</topic><topic>Receptors, Leptin</topic><topic>Reverse transcriptase polymerase chain reaction</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA Splicing</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Rodents</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hoggard, Nigel</creatorcontrib><creatorcontrib>Hunter, Leif</creatorcontrib><creatorcontrib>Duncan, Jacqueline S.</creatorcontrib><creatorcontrib>Williams, Lynda M.</creatorcontrib><creatorcontrib>Trayhurn, Paul</creatorcontrib><creatorcontrib>Mercer, Julian G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hoggard, Nigel</au><au>Hunter, Leif</au><au>Duncan, Jacqueline S.</au><au>Williams, Lynda M.</au><au>Trayhurn, Paul</au><au>Mercer, Julian G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Leptin and Leptin Receptor mRNA and Protein Expression in the Murine Fetus and Placenta</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1997-09-30</date><risdate>1997</risdate><volume>94</volume><issue>20</issue><spage>11073</spage><epage>11078</epage><pages>11073-11078</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Leptin is a 167-aa protein that is secreted from adipose tissue and is important in the regulation of energy balance. It also functions in hematopoiesis and reproduction. To assess whether leptin is involved in fetal growth and development we have examined the distribution of mRNAs encoding leptin and the leptin receptor (which has at least six splice variants) in the 14.5-day postcoitus mouse fetus and in the placenta using reverse transcription-PCR and in situ hybridization. High levels of gene expression for leptin, the leptin receptor, and the long splice variant of the leptin receptor with an intracellular signaling domain were observed in the placenta, fetal cartilage/bone, and hair follicles. Receptor expression also was detected in the lung, as well as the leptomeninges and choroid plexus of the fetal brain. Western blotting and immunocytochemistry, using specific antibodies, demonstrated the presence of leptin and leptin receptor protein in these tissues. These results suggest that leptin may play a role in the growth and development of the fetus, both through placental and fetal expression of the leptin and leptin receptor genes. In the fetus, leptin may be multifunctional and have both paracrine and endocrine effects.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>9380761</pmid><doi>10.1073/pnas.94.20.11073</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Antiserum Biological Sciences Blotting, Western Carrier Proteins - genetics Carrier Proteins - metabolism Complementary DNA Female Fetus Fetus - metabolism Fetuses Genetics Immunohistochemistry In Situ Hybridization Leptin Lungs Medical research Messenger RNA Mice Placenta Placenta - metabolism Polymerase Chain Reaction Pregnancy Proteins - genetics Proteins - metabolism Receptors Receptors, Cell Surface Receptors, Leptin Reverse transcriptase polymerase chain reaction Ribonucleic acid RNA RNA Splicing RNA, Messenger - genetics RNA, Messenger - metabolism Rodents |
| title | Leptin and Leptin Receptor mRNA and Protein Expression in the Murine Fetus and Placenta |
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