TAFII mutations disrupt Dorsal activation in the Drosophila embryo
In this study, we present evidence that the Dorsal activator interacts with limiting amounts of the TFIID complex in the Drosophila embryo. In vitro transcription reactions and protein binding assays implicate the TAF II 110 and TAF II 60 subunits of the TFIID complex in contributing to Dorsal-media...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1998-11, Vol.95 (23), p.13483-13488 |
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| container_end_page | 13488 |
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| container_issue | 23 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 95 |
| creator | Zhou, J Zwicker, J Szymanski, P Levine, M Tjian, R |
| description | In this study, we present evidence that the Dorsal activator interacts with limiting amounts of the TFIID complex in the Drosophila embryo. In vitro transcription reactions and protein binding assays implicate the TAF II 110 and TAF II 60 subunits of the TFIID complex in contributing to Dorsal-mediated activation. Mutations in TAF II 110 and TAF II 60 result in altered patterns of snail and twist transcription in embryos derived from dl /+ females. These results suggest that TAF II s contribute to the activation of transcription in vivo and support the hypothesis that subunits of TFIID may serve as targets of enhancer binding proteins. |
| doi_str_mv | 10.1073/pnas.95.23.13483 |
| format | Article |
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Zwicker, J ; Szymanski, P ; Levine, M ; Tjian, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p258t-1132b029a7aba60e3cca14860e560f4840976a10127872494dd36c5b6ac1e4123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Biological Sciences</topic><topic>Drosophila - embryology</topic><topic>Drosophila - genetics</topic><topic>Drosophila Proteins</topic><topic>Embryos</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Genetics</topic><topic>Insects</topic><topic>Mutation</topic><topic>Nuclear Proteins - genetics</topic><topic>Phosphoproteins - genetics</topic><topic>Proteins</topic><topic>Transcription Factor TFIID</topic><topic>Transcription Factors</topic><topic>Transcription Factors, TFII - genetics</topic><topic>Transcriptional Activation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhou, J</creatorcontrib><creatorcontrib>Zwicker, J</creatorcontrib><creatorcontrib>Szymanski, P</creatorcontrib><creatorcontrib>Levine, M</creatorcontrib><creatorcontrib>Tjian, R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, J</au><au>Zwicker, J</au><au>Szymanski, P</au><au>Levine, M</au><au>Tjian, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TAFII mutations disrupt Dorsal activation in the Drosophila embryo</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1998-11-10</date><risdate>1998</risdate><volume>95</volume><issue>23</issue><spage>13483</spage><epage>13488</epage><pages>13483-13488</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>In this study, we present evidence that the Dorsal activator interacts with limiting amounts of the TFIID complex in the Drosophila embryo. 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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1998-11, Vol.95 (23), p.13483-13488 |
| issn | 0027-8424 1091-6490 |
| language | eng |
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| source | MEDLINE; Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Biological Sciences Drosophila - embryology Drosophila - genetics Drosophila Proteins Embryos Gene Expression Regulation, Developmental Genetics Insects Mutation Nuclear Proteins - genetics Phosphoproteins - genetics Proteins Transcription Factor TFIID Transcription Factors Transcription Factors, TFII - genetics Transcriptional Activation |
| title | TAFII mutations disrupt Dorsal activation in the Drosophila embryo |
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