Active barnase variants with completely random hydrophonic cores

Active barnase variants with completely random hydrophonic cores

To test the minimum requirements for a core to provide sufficient structural integrity for enzymatic activity, Axe et al produced mutants of the ribonuclease barnase in which 12 of the 13 core residues have been randomly replaced by hydrophonic alternatives. A strikingly high proportion of these mut...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1996-05, Vol.93 (11), p.5590
Hauptverfasser: Axe, Douglas D, Foster, Nicholas W, Fersht, Alan R
Format: Artikel
Sprache:eng
Schlagworte:
Biochemistry
Enzymes
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:To test the minimum requirements for a core to provide sufficient structural integrity for enzymatic activity, Axe et al produced mutants of the ribonuclease barnase in which 12 of the 13 core residues have been randomly replaced by hydrophonic alternatives. A strikingly high proportion of these mutants (23%) retained enzymatic activity in vivo.
ISSN:0027-8424
1091-6490