Direct observation of twisting steps during Rad51 polymerization on DNA

The human recombinase hRad51 is a key protein for the maintenance of genome integrity and for cancer development. Polymerization and depolymerization of hRad51 on duplex DNA were studied here using a new generation of magnetic tweezers, measuring DNA twist in real time with a resolution of 5°. Our r...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2009-11, Vol.106 (46), p.19239-19244
Hauptverfasser:	Arata, Hideyuki, Dupont, Aurélie, Miné-Hattab, Judith, Disseau, Ludovic, Renodon-Cornière, Axelle, Takahashi, Masayuki, Viovy, Jean-Louis, Cappello, Giovanni
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemistry, Molecular Biology Biological Sciences Biophysics Deoxyribonucleic acid Depolymerization DNA DNA - chemistry DNA - metabolism Humans Kinetics Life Sciences Magnetic fields Magnetics Molecules Monomers Nucleation Nucleic Acid Conformation Nucleoproteins Nucleoproteins - chemistry Nucleoproteins - metabolism Physical Sciences Polymerization Polymers - chemistry Polymers - metabolism Proteins Rad51 Recombinase - chemistry Rad51 Recombinase - metabolism Rotation Simulation
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