Fos and Jun Do Not Bend the AP-1 Recognition Site

We have used a solution-based DNA cyclization assay and a gel-phasing method to show that contrary to previous reports [Kerppola, T. K. & Curran, T. (1991) Cell 66, 317-326], basic region leucine zipper proteins Fos and Jun do not significantly bend their AP-1 recognition site. We have construct...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1996-04, Vol.93 (8), p.3248-3252
Hauptverfasser:	Sitlani, Ayesha, Crothers, Donald M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Basic leucine zipper transcription factors Bending Binding Sites Biochemistry Collagenases - genetics Curvature Deoxyribonucleic acid DNA DNA, Recombinant - chemistry DNA, Recombinant - genetics DNA, Recombinant - metabolism Gels Humans In Vitro Techniques Kinetics Leucine Zippers Ligation Molecular Sequence Data Molecules Nucleic Acid Conformation Protein Binding Proteins Proto-Oncogene Proteins c-fos - metabolism Proto-Oncogene Proteins c-jun - metabolism Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Transcription Factor AP-1 - genetics Transcription Factor AP-1 - metabolism Yeasts
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