Nuclear localization of prostaglandin E2 receptors
Prostaglandin E 2 receptors (EP) were detected by radioligand binding in nuclear fractions isolated from porcine brain and myometrium. Intracellular localization by immunocytofluorescence revealed perinuclear localization of EPs in porcine cerebral microvascular endothelial cells. Nuclear associatio...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1998-12, Vol.95 (26), p.15792-15797 |
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| container_issue | 26 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 95 |
| creator | Bhattacharya, M Peri, K G Almazan, G Ribeiro-da-Silva, A Shichi, H Durocher, Y Abramovitz, M Hou, X Varma, D R Chemtob, S |
| description | Prostaglandin E 2 receptors (EP) were detected by radioligand binding in nuclear fractions isolated from porcine brain and myometrium. Intracellular localization by immunocytofluorescence revealed perinuclear localization of EPs in porcine cerebral microvascular endothelial cells. Nuclear association of EP 1 was also found in fibroblast Swiss 3T3 cells stably overexpressing EP 1 and in human embryonic kidney 293 (Epstein–Barr virus-encoded nuclear antigen) cells expressing EP 1 fused to green fluorescent protein. High-resolution immunostaining of EP 1 revealed their presence in the nuclear envelope of isolated (cultured) endothelial cells and in situ in brain (cortex) endothelial cells and neurons. Stimulation of these nuclear receptors modulate nuclear calcium and gene transcription. |
| doi_str_mv | 10.1073/pnas.95.26.15792 |
| format | Article |
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Intracellular localization by immunocytofluorescence revealed perinuclear localization of EPs in porcine cerebral microvascular endothelial cells. Nuclear association of EP 1 was also found in fibroblast Swiss 3T3 cells stably overexpressing EP 1 and in human embryonic kidney 293 (Epstein–Barr virus-encoded nuclear antigen) cells expressing EP 1 fused to green fluorescent protein. High-resolution immunostaining of EP 1 revealed their presence in the nuclear envelope of isolated (cultured) endothelial cells and in situ in brain (cortex) endothelial cells and neurons. Stimulation of these nuclear receptors modulate nuclear calcium and gene transcription.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.95.26.15792</identifier><identifier>PMID: 9861049</identifier><language>eng</language><publisher>United States: National Acad Sciences</publisher><subject>3T3 Cells ; Animals ; Animals, Newborn ; Binding, Competitive ; Biological Sciences ; Cell Line ; Cell Nucleus - metabolism ; Cell Nucleus - ultrastructure ; Cerebrovascular Circulation ; Dinoprostone - metabolism ; Endothelium, Vascular - metabolism ; Epstein-Barr Virus Nuclear Antigens - genetics ; Epstein-Barr Virus Nuclear Antigens - metabolism ; Green Fluorescent Proteins ; Humans ; Kidney ; Luminescent Proteins - genetics ; Luminescent Proteins - metabolism ; Mice ; Microcirculation ; Microscopy, Electron ; Pharmacology ; Receptors, Prostaglandin E - genetics ; Receptors, Prostaglandin E - metabolism ; Recombinant Fusion Proteins - metabolism ; Swine</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1998-12, Vol.95 (26), p.15792-15797</ispartof><rights>Copyright National Academy of Sciences Dec 22, 1998</rights><rights>Copyright © 1998, The National Academy of Sciences 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/95/26.cover.gif</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC28123/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC28123/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9861049$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bhattacharya, M</creatorcontrib><creatorcontrib>Peri, K G</creatorcontrib><creatorcontrib>Almazan, G</creatorcontrib><creatorcontrib>Ribeiro-da-Silva, A</creatorcontrib><creatorcontrib>Shichi, H</creatorcontrib><creatorcontrib>Durocher, Y</creatorcontrib><creatorcontrib>Abramovitz, M</creatorcontrib><creatorcontrib>Hou, X</creatorcontrib><creatorcontrib>Varma, D R</creatorcontrib><creatorcontrib>Chemtob, S</creatorcontrib><title>Nuclear localization of prostaglandin E2 receptors</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Prostaglandin E 2 receptors (EP) were detected by radioligand binding in nuclear fractions isolated from porcine brain and myometrium. Intracellular localization by immunocytofluorescence revealed perinuclear localization of EPs in porcine cerebral microvascular endothelial cells. Nuclear association of EP 1 was also found in fibroblast Swiss 3T3 cells stably overexpressing EP 1 and in human embryonic kidney 293 (Epstein–Barr virus-encoded nuclear antigen) cells expressing EP 1 fused to green fluorescent protein. High-resolution immunostaining of EP 1 revealed their presence in the nuclear envelope of isolated (cultured) endothelial cells and in situ in brain (cortex) endothelial cells and neurons. 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Intracellular localization by immunocytofluorescence revealed perinuclear localization of EPs in porcine cerebral microvascular endothelial cells. Nuclear association of EP 1 was also found in fibroblast Swiss 3T3 cells stably overexpressing EP 1 and in human embryonic kidney 293 (Epstein–Barr virus-encoded nuclear antigen) cells expressing EP 1 fused to green fluorescent protein. High-resolution immunostaining of EP 1 revealed their presence in the nuclear envelope of isolated (cultured) endothelial cells and in situ in brain (cortex) endothelial cells and neurons. Stimulation of these nuclear receptors modulate nuclear calcium and gene transcription.</abstract><cop>United States</cop><pub>National Acad Sciences</pub><pmid>9861049</pmid><doi>10.1073/pnas.95.26.15792</doi><tpages>6</tpages></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1998-12, Vol.95 (26), p.15792-15797 |
| issn | 0027-8424 1091-6490 |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | 3T3 Cells Animals Animals, Newborn Binding, Competitive Biological Sciences Cell Line Cell Nucleus - metabolism Cell Nucleus - ultrastructure Cerebrovascular Circulation Dinoprostone - metabolism Endothelium, Vascular - metabolism Epstein-Barr Virus Nuclear Antigens - genetics Epstein-Barr Virus Nuclear Antigens - metabolism Green Fluorescent Proteins Humans Kidney Luminescent Proteins - genetics Luminescent Proteins - metabolism Mice Microcirculation Microscopy, Electron Pharmacology Receptors, Prostaglandin E - genetics Receptors, Prostaglandin E - metabolism Recombinant Fusion Proteins - metabolism Swine |
| title | Nuclear localization of prostaglandin E2 receptors |
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