Crystallization and Identification of an Assembly Defect of Recombinant Antenna Complexes Produced in Transgenic Tobacco Plants

A chimeric Lhcb gene encoding light-harvesting chlorophyll a/b-binding protein (LHCII) was expressed in transgenic tobacco plants. To separate native from recombinant LHCII, the protein was extended by six histidines at its C terminus. Recombinant LHCII was isolated by detergent-mediated monomerizat...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1996-12, Vol.93 (25), p.14966-14971
Hauptverfasser:	Flachmann, Ralf, Kuhlbrandt, Werner
Format:	Artikel
Sprache:	eng
Schlagworte:	Arabidopsis Proteins Base Sequence Biological Sciences Chlorophyll Binding Proteins Crystallization Crystals Detergents Gels Genes, Plant Light-Harvesting Protein Complexes Lipids Molecular Sequence Data Monomers Nicotiana - genetics Nicotiana tabacum Photosynthetic Reaction Center Complex Proteins - chemistry Photosynthetic Reaction Center Complex Proteins - genetics Photosystem I Protein Complex Plant Proteins Plants Plants, Genetically Modified Plants, Toxic Proteins Recombinant Proteins - chemistry Recombinant Proteins - genetics Thylakoids Transgenic plants Trimers
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