Developmental Expression of Synthetic Cis-Regulatory Systems Composed of Spatial Control Elements from Two Different Genes
Synthetic cis-regulatory systems consisting of positively and negatively acting cis-regulatory modules of the Endo16 gene were combined with the lineage-specific regulatory element of the SM50 gene associated with a reporter and injected into eggs of sea urchins. We show here that synthetic cis-regu...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1996-11, Vol.93 (24), p.13849-13854 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Kirchhamer, Carmen V. Bogarad, Leonard D. Davidson, Eric H. |
| description | Synthetic cis-regulatory systems consisting of positively and negatively acting cis-regulatory modules of the Endo16 gene were combined with the lineage-specific regulatory element of the SM50 gene associated with a reporter and injected into eggs of sea urchins. We show here that synthetic cis-regulatory systems consisting of the positive Endo16 regulatory elements linked with the SM50 regulatory element are expressed spatially exactly as the sum of the individual endodermal and skeletogenic expression patterns. In combination, both lineage-specific positive regulatory elements function autonomously. However, addition of the Endo16 regulatory module that represses ectopic skeletogenic expression of Endo16 receptor constructs does not affect expression driven by the SM50 regulatory elements in the same skeletogenic cells. The repression function of this element is thus dedicated to control of the positive spatial output of the Endo16 regulatory system. |
| doi_str_mv | 10.1073/pnas.93.24.13849 |
| format | Article |
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We show here that synthetic cis-regulatory systems consisting of the positive Endo16 regulatory elements linked with the SM50 regulatory element are expressed spatially exactly as the sum of the individual endodermal and skeletogenic expression patterns. In combination, both lineage-specific positive regulatory elements function autonomously. However, addition of the Endo16 regulatory module that represses ectopic skeletogenic expression of Endo16 receptor constructs does not affect expression driven by the SM50 regulatory elements in the same skeletogenic cells. The repression function of this element is thus dedicated to control of the positive spatial output of the Endo16 regulatory system.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.93.24.13849</identifier><identifier>PMID: 8943024</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Biological Sciences ; Biology ; Cell Adhesion Molecules - biosynthesis ; Cell Adhesion Molecules - genetics ; Cell lines ; Chloramphenicol O-Acetyltransferase - biosynthesis ; DNA Primers ; Ectoderm ; Embryo, Nonmammalian - cytology ; Embryo, Nonmammalian - physiology ; Embryos ; Endoderm ; Female ; Gene Expression Regulation, Developmental ; Genes ; Genes, Reporter ; Genetics ; In situ hybridization ; Marine ; Oocytes - physiology ; Plasmids ; Protein Biosynthesis ; Proteins - genetics ; Recombinant Fusion Proteins - biosynthesis ; Regulatory Sequences, Nucleic Acid ; Reporter genes ; Restriction Mapping ; Sea Urchins - genetics ; Sea Urchins - growth & development ; Stripes ; Strongylocentrotus purpuratus</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1996-11, Vol.93 (24), p.13849-13854</ispartof><rights>Copyright 1996 National Academy of Sciences</rights><rights>Copyright National Academy of Sciences Nov 26, 1996</rights><rights>Copyright © 1996, The National Academy of Sciences of the USA 1996</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c523t-528aab823652b4c460a8fb9014e1405330e87ec3aa448dff07fe873276af79a13</citedby><cites>FETCH-LOGICAL-c523t-528aab823652b4c460a8fb9014e1405330e87ec3aa448dff07fe873276af79a13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/93/24.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/40990$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/40990$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,315,728,781,785,804,886,27929,27930,53796,53798,58022,58255</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8943024$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kirchhamer, Carmen V.</creatorcontrib><creatorcontrib>Bogarad, Leonard D.</creatorcontrib><creatorcontrib>Davidson, Eric H.</creatorcontrib><title>Developmental Expression of Synthetic Cis-Regulatory Systems Composed of Spatial Control Elements from Two Different Genes</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Synthetic cis-regulatory systems consisting of positively and negatively acting cis-regulatory modules of the Endo16 gene were combined with the lineage-specific regulatory element of the SM50 gene associated with a reporter and injected into eggs of sea urchins. We show here that synthetic cis-regulatory systems consisting of the positive Endo16 regulatory elements linked with the SM50 regulatory element are expressed spatially exactly as the sum of the individual endodermal and skeletogenic expression patterns. In combination, both lineage-specific positive regulatory elements function autonomously. However, addition of the Endo16 regulatory module that represses ectopic skeletogenic expression of Endo16 receptor constructs does not affect expression driven by the SM50 regulatory elements in the same skeletogenic cells. The repression function of this element is thus dedicated to control of the positive spatial output of the Endo16 regulatory system.</description><subject>Animals</subject><subject>Biological Sciences</subject><subject>Biology</subject><subject>Cell Adhesion Molecules - biosynthesis</subject><subject>Cell Adhesion Molecules - genetics</subject><subject>Cell lines</subject><subject>Chloramphenicol O-Acetyltransferase - biosynthesis</subject><subject>DNA Primers</subject><subject>Ectoderm</subject><subject>Embryo, Nonmammalian - cytology</subject><subject>Embryo, Nonmammalian - physiology</subject><subject>Embryos</subject><subject>Endoderm</subject><subject>Female</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Genes</subject><subject>Genes, Reporter</subject><subject>Genetics</subject><subject>In situ hybridization</subject><subject>Marine</subject><subject>Oocytes - physiology</subject><subject>Plasmids</subject><subject>Protein Biosynthesis</subject><subject>Proteins - genetics</subject><subject>Recombinant Fusion Proteins - biosynthesis</subject><subject>Regulatory Sequences, Nucleic Acid</subject><subject>Reporter genes</subject><subject>Restriction Mapping</subject><subject>Sea Urchins - genetics</subject><subject>Sea Urchins - growth & development</subject><subject>Stripes</subject><subject>Strongylocentrotus purpuratus</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks1v1DAQxS0EKtvCHSEhIg6IS5bxRxJb4oK2pSBVQoJytrzZcZtVEqe2U7r963F2l1XhACdL837vaexnQl5QmFOo-PuhN2Gu-JyJOeVSqEdkRkHRvBQKHpMZAKtyKZh4So5DWAOAKiQckSOpBAcmZuT-FG-xdUOHfTRtdnY3eAyhcX3mbPZ908drjE2dLZqQf8OrsTXR-U0SQsQuZAvXDS7gagsPJjYpYuH66F2KanEKDZn1rssuf7rstLEWfZpl59hjeEaeWNMGfL4_T8iPT2eXi8_5xdfzL4uPF3ldMB7zgkljlpLxsmBLUYsSjLRLBVQgFVBwDigrrLkxQsiVtVDZNOCsKo2tlKH8hHzY5Q7jssNVnRbwptWDbzrjN9qZRv-p9M21vnK3miohimR_u7d7dzNiiLprQo1ta3p0Y9CVLKqSSf5fkBaVKhmbFnrzF7h2o-_TG2gGlElZbCHYQbV3IXi0h4Up6Kl7PXWvFddM6G33yfLq4UUPhn3ZSX-31yfnb_VBgrZj20a8iwl9_W80ES93xDqkL3FABCgF_BdScM5d</recordid><startdate>19961126</startdate><enddate>19961126</enddate><creator>Kirchhamer, Carmen V.</creator><creator>Bogarad, Leonard D.</creator><creator>Davidson, Eric H.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><general>The National Academy of Sciences of the USA</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>F1W</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19961126</creationdate><title>Developmental Expression of Synthetic Cis-Regulatory Systems Composed of Spatial Control Elements from Two Different Genes</title><author>Kirchhamer, Carmen V. ; Bogarad, Leonard D. ; Davidson, Eric H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c523t-528aab823652b4c460a8fb9014e1405330e87ec3aa448dff07fe873276af79a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Biological Sciences</topic><topic>Biology</topic><topic>Cell Adhesion Molecules - biosynthesis</topic><topic>Cell Adhesion Molecules - genetics</topic><topic>Cell lines</topic><topic>Chloramphenicol O-Acetyltransferase - biosynthesis</topic><topic>DNA Primers</topic><topic>Ectoderm</topic><topic>Embryo, Nonmammalian - cytology</topic><topic>Embryo, Nonmammalian - physiology</topic><topic>Embryos</topic><topic>Endoderm</topic><topic>Female</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Genes</topic><topic>Genes, Reporter</topic><topic>Genetics</topic><topic>In situ hybridization</topic><topic>Marine</topic><topic>Oocytes - physiology</topic><topic>Plasmids</topic><topic>Protein Biosynthesis</topic><topic>Proteins - genetics</topic><topic>Recombinant Fusion Proteins - biosynthesis</topic><topic>Regulatory Sequences, Nucleic Acid</topic><topic>Reporter genes</topic><topic>Restriction Mapping</topic><topic>Sea Urchins - genetics</topic><topic>Sea Urchins - growth & development</topic><topic>Stripes</topic><topic>Strongylocentrotus purpuratus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kirchhamer, Carmen V.</creatorcontrib><creatorcontrib>Bogarad, Leonard D.</creatorcontrib><creatorcontrib>Davidson, Eric H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kirchhamer, Carmen V.</au><au>Bogarad, Leonard D.</au><au>Davidson, Eric H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Developmental Expression of Synthetic Cis-Regulatory Systems Composed of Spatial Control Elements from Two Different Genes</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1996-11-26</date><risdate>1996</risdate><volume>93</volume><issue>24</issue><spage>13849</spage><epage>13854</epage><pages>13849-13854</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Synthetic cis-regulatory systems consisting of positively and negatively acting cis-regulatory modules of the Endo16 gene were combined with the lineage-specific regulatory element of the SM50 gene associated with a reporter and injected into eggs of sea urchins. We show here that synthetic cis-regulatory systems consisting of the positive Endo16 regulatory elements linked with the SM50 regulatory element are expressed spatially exactly as the sum of the individual endodermal and skeletogenic expression patterns. In combination, both lineage-specific positive regulatory elements function autonomously. However, addition of the Endo16 regulatory module that represses ectopic skeletogenic expression of Endo16 receptor constructs does not affect expression driven by the SM50 regulatory elements in the same skeletogenic cells. The repression function of this element is thus dedicated to control of the positive spatial output of the Endo16 regulatory system.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>8943024</pmid><doi>10.1073/pnas.93.24.13849</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1996-11, Vol.93 (24), p.13849-13854 |
| issn | 0027-8424 1091-6490 |
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| subjects | Animals Biological Sciences Biology Cell Adhesion Molecules - biosynthesis Cell Adhesion Molecules - genetics Cell lines Chloramphenicol O-Acetyltransferase - biosynthesis DNA Primers Ectoderm Embryo, Nonmammalian - cytology Embryo, Nonmammalian - physiology Embryos Endoderm Female Gene Expression Regulation, Developmental Genes Genes, Reporter Genetics In situ hybridization Marine Oocytes - physiology Plasmids Protein Biosynthesis Proteins - genetics Recombinant Fusion Proteins - biosynthesis Regulatory Sequences, Nucleic Acid Reporter genes Restriction Mapping Sea Urchins - genetics Sea Urchins - growth & development Stripes Strongylocentrotus purpuratus |
| title | Developmental Expression of Synthetic Cis-Regulatory Systems Composed of Spatial Control Elements from Two Different Genes |
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