A multiscale study of the role of dynamin in the regulation of glucose uptake
Glucose uptake in muscle cells in response to insulin is a fundamental mechanism for metabolism. The inability of cells to mobilize the specific glucose transporter GLUT4 is believed to be at least partially accountable for diseases, like diabetes, where cells do not respond to an insulin stimulus....
Gespeichert in:
| Veröffentlicht in: | Integrative biology (Cambridge) 2017-10, Vol.9 (1), p.81-819 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 819 |
|---|---|
| container_issue | 1 |
| container_start_page | 81 |
| container_title | Integrative biology (Cambridge) |
| container_volume | 9 |
| creator | Trouillon, Raphaël Letizia, M. Cristina Menzies, Keir J Mouchiroud, Laurent Auwerx, Johan Schoonjans, Kristina Gijs, Martin A. M |
| description | Glucose uptake in muscle cells in response to insulin is a fundamental mechanism for metabolism. The inability of cells to mobilize the specific glucose transporter GLUT4 is believed to be at least partially accountable for diseases, like diabetes, where cells do not respond to an insulin stimulus. In this work, a microchip is used to detect electrochemically glucose uptake from C2C12 myoblasts cultured on a patch of paper upon exposure to insulin. More importantly, the data suggest a new role for dynamin, a molecular motor which would be involved in GLUT4 translocation by facilitating exocytosis. It is also shown
in vivo
that dynamin is involved in the response to glucose in a completely distinct organism, namely the nematode
Caenorhabditis elegans
. The new mechanism for dynamin could therefore be more generally relevant
in vivo
and may play a role in insulin resistance.
Cells- and organisms-on-a-chip strategies were used to highlight the role of the molecular motor dynamin in regulating the translocation of specific glucose transporters. |
| doi_str_mv | 10.1039/c7ib00015d |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2010867980</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2010867980</sourcerecordid><originalsourceid>FETCH-LOGICAL-c373t-fef6cbabc16506268e2825b330da7ca9d852fae2b6be1cce41788b5371ab9463</originalsourceid><addsrcrecordid>eNpdkctLAzEQxoMotlYv3pUFLyKsJpvN61jrq1Dx0vuSZLN16-6mJptD_3vThxWEgRlmfgzffAPAJYL3CGLxoFmtIISIlEdgiBhhqWCQH__WVOQDcOb9EkKaQ5ifgkHGOcGM4CF4HydtaPraa9mYxPehXCe2SvpPkzgbO7Eu151s6y6JsW2bRWhkX9tuM1w0QVtvkrDq5Zc5ByeVbLy52OcRmL88zydv6ezjdToZz1KNGe7TylRUK6k0ogTSjHKT8YwojGEpmZai5CSrpMkUVQZpbXLEOFdRMZJK5BSPwO1u7crZ72B8X7TxANM0sjM2-AIJjAWmhIuI3vxDlza4LoorMoggp0xwGKm7HaWd9d6Zqli5upVuXSBYbDwuJmz6uPX4KcLX-5VBtaY8oL-mRuBqBzivD9O_J-EfZJZ_3w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2010867980</pqid></control><display><type>article</type><title>A multiscale study of the role of dynamin in the regulation of glucose uptake</title><source>MEDLINE</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Trouillon, Raphaël ; Letizia, M. Cristina ; Menzies, Keir J ; Mouchiroud, Laurent ; Auwerx, Johan ; Schoonjans, Kristina ; Gijs, Martin A. M</creator><creatorcontrib>Trouillon, Raphaël ; Letizia, M. Cristina ; Menzies, Keir J ; Mouchiroud, Laurent ; Auwerx, Johan ; Schoonjans, Kristina ; Gijs, Martin A. M</creatorcontrib><description>Glucose uptake in muscle cells in response to insulin is a fundamental mechanism for metabolism. The inability of cells to mobilize the specific glucose transporter GLUT4 is believed to be at least partially accountable for diseases, like diabetes, where cells do not respond to an insulin stimulus. In this work, a microchip is used to detect electrochemically glucose uptake from C2C12 myoblasts cultured on a patch of paper upon exposure to insulin. More importantly, the data suggest a new role for dynamin, a molecular motor which would be involved in GLUT4 translocation by facilitating exocytosis. It is also shown
in vivo
that dynamin is involved in the response to glucose in a completely distinct organism, namely the nematode
Caenorhabditis elegans
. The new mechanism for dynamin could therefore be more generally relevant
in vivo
and may play a role in insulin resistance.
Cells- and organisms-on-a-chip strategies were used to highlight the role of the molecular motor dynamin in regulating the translocation of specific glucose transporters.</description><identifier>ISSN: 1757-9694</identifier><identifier>EISSN: 1757-9708</identifier><identifier>DOI: 10.1039/c7ib00015d</identifier><identifier>PMID: 28853753</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Animals ; Behavior, Animal ; Biological Transport ; Caenorhabditis elegans - metabolism ; Cell Line ; Diabetes mellitus ; Dynamin ; Dynamins - physiology ; Electrochemistry ; Equipment Design ; Exocytosis ; Glucose ; Glucose - metabolism ; Glucose transporter ; Glucose Transporter Type 4 - metabolism ; Insulin ; Insulin - metabolism ; Insulin resistance ; Metabolism ; Mice ; Multiscale analysis ; Muscle, Skeletal - metabolism ; Muscles ; Myoblasts ; Myoblasts - metabolism ; Nematodes ; Protein Transport ; Translocation</subject><ispartof>Integrative biology (Cambridge), 2017-10, Vol.9 (1), p.81-819</ispartof><rights>Copyright Royal Society of Chemistry 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c373t-fef6cbabc16506268e2825b330da7ca9d852fae2b6be1cce41788b5371ab9463</citedby><cites>FETCH-LOGICAL-c373t-fef6cbabc16506268e2825b330da7ca9d852fae2b6be1cce41788b5371ab9463</cites><orcidid>0000-0002-1873-8500 ; 0000-0003-1743-5302 ; 0000-0002-8735-9547</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28853753$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Trouillon, Raphaël</creatorcontrib><creatorcontrib>Letizia, M. Cristina</creatorcontrib><creatorcontrib>Menzies, Keir J</creatorcontrib><creatorcontrib>Mouchiroud, Laurent</creatorcontrib><creatorcontrib>Auwerx, Johan</creatorcontrib><creatorcontrib>Schoonjans, Kristina</creatorcontrib><creatorcontrib>Gijs, Martin A. M</creatorcontrib><title>A multiscale study of the role of dynamin in the regulation of glucose uptake</title><title>Integrative biology (Cambridge)</title><addtitle>Integr Biol (Camb)</addtitle><description>Glucose uptake in muscle cells in response to insulin is a fundamental mechanism for metabolism. The inability of cells to mobilize the specific glucose transporter GLUT4 is believed to be at least partially accountable for diseases, like diabetes, where cells do not respond to an insulin stimulus. In this work, a microchip is used to detect electrochemically glucose uptake from C2C12 myoblasts cultured on a patch of paper upon exposure to insulin. More importantly, the data suggest a new role for dynamin, a molecular motor which would be involved in GLUT4 translocation by facilitating exocytosis. It is also shown
in vivo
that dynamin is involved in the response to glucose in a completely distinct organism, namely the nematode
Caenorhabditis elegans
. The new mechanism for dynamin could therefore be more generally relevant
in vivo
and may play a role in insulin resistance.
Cells- and organisms-on-a-chip strategies were used to highlight the role of the molecular motor dynamin in regulating the translocation of specific glucose transporters.</description><subject>Animals</subject><subject>Behavior, Animal</subject><subject>Biological Transport</subject><subject>Caenorhabditis elegans - metabolism</subject><subject>Cell Line</subject><subject>Diabetes mellitus</subject><subject>Dynamin</subject><subject>Dynamins - physiology</subject><subject>Electrochemistry</subject><subject>Equipment Design</subject><subject>Exocytosis</subject><subject>Glucose</subject><subject>Glucose - metabolism</subject><subject>Glucose transporter</subject><subject>Glucose Transporter Type 4 - metabolism</subject><subject>Insulin</subject><subject>Insulin - metabolism</subject><subject>Insulin resistance</subject><subject>Metabolism</subject><subject>Mice</subject><subject>Multiscale analysis</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Muscles</subject><subject>Myoblasts</subject><subject>Myoblasts - metabolism</subject><subject>Nematodes</subject><subject>Protein Transport</subject><subject>Translocation</subject><issn>1757-9694</issn><issn>1757-9708</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkctLAzEQxoMotlYv3pUFLyKsJpvN61jrq1Dx0vuSZLN16-6mJptD_3vThxWEgRlmfgzffAPAJYL3CGLxoFmtIISIlEdgiBhhqWCQH__WVOQDcOb9EkKaQ5ifgkHGOcGM4CF4HydtaPraa9mYxPehXCe2SvpPkzgbO7Eu151s6y6JsW2bRWhkX9tuM1w0QVtvkrDq5Zc5ByeVbLy52OcRmL88zydv6ezjdToZz1KNGe7TylRUK6k0ogTSjHKT8YwojGEpmZai5CSrpMkUVQZpbXLEOFdRMZJK5BSPwO1u7crZ72B8X7TxANM0sjM2-AIJjAWmhIuI3vxDlza4LoorMoggp0xwGKm7HaWd9d6Zqli5upVuXSBYbDwuJmz6uPX4KcLX-5VBtaY8oL-mRuBqBzivD9O_J-EfZJZ_3w</recordid><startdate>20171016</startdate><enddate>20171016</enddate><creator>Trouillon, Raphaël</creator><creator>Letizia, M. Cristina</creator><creator>Menzies, Keir J</creator><creator>Mouchiroud, Laurent</creator><creator>Auwerx, Johan</creator><creator>Schoonjans, Kristina</creator><creator>Gijs, Martin A. M</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1873-8500</orcidid><orcidid>https://orcid.org/0000-0003-1743-5302</orcidid><orcidid>https://orcid.org/0000-0002-8735-9547</orcidid></search><sort><creationdate>20171016</creationdate><title>A multiscale study of the role of dynamin in the regulation of glucose uptake</title><author>Trouillon, Raphaël ; Letizia, M. Cristina ; Menzies, Keir J ; Mouchiroud, Laurent ; Auwerx, Johan ; Schoonjans, Kristina ; Gijs, Martin A. M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c373t-fef6cbabc16506268e2825b330da7ca9d852fae2b6be1cce41788b5371ab9463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Behavior, Animal</topic><topic>Biological Transport</topic><topic>Caenorhabditis elegans - metabolism</topic><topic>Cell Line</topic><topic>Diabetes mellitus</topic><topic>Dynamin</topic><topic>Dynamins - physiology</topic><topic>Electrochemistry</topic><topic>Equipment Design</topic><topic>Exocytosis</topic><topic>Glucose</topic><topic>Glucose - metabolism</topic><topic>Glucose transporter</topic><topic>Glucose Transporter Type 4 - metabolism</topic><topic>Insulin</topic><topic>Insulin - metabolism</topic><topic>Insulin resistance</topic><topic>Metabolism</topic><topic>Mice</topic><topic>Multiscale analysis</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Muscles</topic><topic>Myoblasts</topic><topic>Myoblasts - metabolism</topic><topic>Nematodes</topic><topic>Protein Transport</topic><topic>Translocation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Trouillon, Raphaël</creatorcontrib><creatorcontrib>Letizia, M. Cristina</creatorcontrib><creatorcontrib>Menzies, Keir J</creatorcontrib><creatorcontrib>Mouchiroud, Laurent</creatorcontrib><creatorcontrib>Auwerx, Johan</creatorcontrib><creatorcontrib>Schoonjans, Kristina</creatorcontrib><creatorcontrib>Gijs, Martin A. M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Integrative biology (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Trouillon, Raphaël</au><au>Letizia, M. Cristina</au><au>Menzies, Keir J</au><au>Mouchiroud, Laurent</au><au>Auwerx, Johan</au><au>Schoonjans, Kristina</au><au>Gijs, Martin A. M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A multiscale study of the role of dynamin in the regulation of glucose uptake</atitle><jtitle>Integrative biology (Cambridge)</jtitle><addtitle>Integr Biol (Camb)</addtitle><date>2017-10-16</date><risdate>2017</risdate><volume>9</volume><issue>1</issue><spage>81</spage><epage>819</epage><pages>81-819</pages><issn>1757-9694</issn><eissn>1757-9708</eissn><abstract>Glucose uptake in muscle cells in response to insulin is a fundamental mechanism for metabolism. The inability of cells to mobilize the specific glucose transporter GLUT4 is believed to be at least partially accountable for diseases, like diabetes, where cells do not respond to an insulin stimulus. In this work, a microchip is used to detect electrochemically glucose uptake from C2C12 myoblasts cultured on a patch of paper upon exposure to insulin. More importantly, the data suggest a new role for dynamin, a molecular motor which would be involved in GLUT4 translocation by facilitating exocytosis. It is also shown
in vivo
that dynamin is involved in the response to glucose in a completely distinct organism, namely the nematode
Caenorhabditis elegans
. The new mechanism for dynamin could therefore be more generally relevant
in vivo
and may play a role in insulin resistance.
Cells- and organisms-on-a-chip strategies were used to highlight the role of the molecular motor dynamin in regulating the translocation of specific glucose transporters.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>28853753</pmid><doi>10.1039/c7ib00015d</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-1873-8500</orcidid><orcidid>https://orcid.org/0000-0003-1743-5302</orcidid><orcidid>https://orcid.org/0000-0002-8735-9547</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1757-9694 |
| ispartof | Integrative biology (Cambridge), 2017-10, Vol.9 (1), p.81-819 |
| issn | 1757-9694 1757-9708 |
| language | eng |
| recordid | cdi_proquest_journals_2010867980 |
| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Animals Behavior, Animal Biological Transport Caenorhabditis elegans - metabolism Cell Line Diabetes mellitus Dynamin Dynamins - physiology Electrochemistry Equipment Design Exocytosis Glucose Glucose - metabolism Glucose transporter Glucose Transporter Type 4 - metabolism Insulin Insulin - metabolism Insulin resistance Metabolism Mice Multiscale analysis Muscle, Skeletal - metabolism Muscles Myoblasts Myoblasts - metabolism Nematodes Protein Transport Translocation |
| title | A multiscale study of the role of dynamin in the regulation of glucose uptake |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T03%3A48%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20multiscale%20study%20of%20the%20role%20of%20dynamin%20in%20the%20regulation%20of%20glucose%20uptake&rft.jtitle=Integrative%20biology%20(Cambridge)&rft.au=Trouillon,%20Rapha%C3%ABl&rft.date=2017-10-16&rft.volume=9&rft.issue=1&rft.spage=81&rft.epage=819&rft.pages=81-819&rft.issn=1757-9694&rft.eissn=1757-9708&rft_id=info:doi/10.1039/c7ib00015d&rft_dat=%3Cproquest_cross%3E2010867980%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2010867980&rft_id=info:pmid/28853753&rfr_iscdi=true |