Detection of procalcitonin (PCT) using the double antibody sandwich method based on fluorescence resonance energy transfer between upconversion nanoparticles and quantum dots
A nanosensor is designed using a double antibody sandwich method based on fluorescence resonance energy transfer (FRET) between NaYF 4 (Yb, Er) upconversion nanoparticles (UCNPs) and CdTe quantum dots (QDs) for the determination of procalcitonin (PCT). The UCNPs (donor) and CdTe QDs (acceptor) were...
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| Veröffentlicht in: | Analytical methods 2018-01, Vol.10 (9), p.1015-1022 |
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| container_title | Analytical methods |
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| creator | Zhou, Yang Shao, Xiangming Han, Yuwang Zhang, Hongman |
| description | A nanosensor is designed using a double antibody sandwich method based on fluorescence resonance energy transfer (FRET) between NaYF
4
(Yb, Er) upconversion nanoparticles (UCNPs) and CdTe quantum dots (QDs) for the determination of procalcitonin (PCT). The UCNPs (donor) and CdTe QDs (acceptor) were modified separately with two PCT monoclonal antibodies, and they were bound together through a specific immune reaction
via
the addition of PCT antigen. The fluorescence intensity of UCNPs was quenched because of the resonant energy transferred between upconversion nanoparticles and quantum dots. Under the optimal conditions, the fluorescence quenching efficiency showed a linear response to the PCT concentration with values ranging from 0.1 to 10 ng mL
−1
and a detection limit of 0.25 ng mL
−1
. This method can be easily extended to the double antibody sandwich assay with potential applications in
in vitro
diagnostics (IVD). |
| doi_str_mv | 10.1039/C8AY00039E |
| format | Article |
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4
(Yb, Er) upconversion nanoparticles (UCNPs) and CdTe quantum dots (QDs) for the determination of procalcitonin (PCT). The UCNPs (donor) and CdTe QDs (acceptor) were modified separately with two PCT monoclonal antibodies, and they were bound together through a specific immune reaction
via
the addition of PCT antigen. The fluorescence intensity of UCNPs was quenched because of the resonant energy transferred between upconversion nanoparticles and quantum dots. Under the optimal conditions, the fluorescence quenching efficiency showed a linear response to the PCT concentration with values ranging from 0.1 to 10 ng mL
−1
and a detection limit of 0.25 ng mL
−1
. This method can be easily extended to the double antibody sandwich assay with potential applications in
in vitro
diagnostics (IVD).</description><identifier>ISSN: 1759-9660</identifier><identifier>EISSN: 1759-9679</identifier><identifier>DOI: 10.1039/C8AY00039E</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>Cadmium tellurides ; Energy transfer ; Erbium ; Fluorescence ; Fluorescence resonance energy transfer ; Monoclonal antibodies ; Nanoparticles ; Procalcitonin ; Quantum dots ; Resonance ; Upconversion ; Ytterbium</subject><ispartof>Analytical methods, 2018-01, Vol.10 (9), p.1015-1022</ispartof><rights>Copyright Royal Society of Chemistry 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c296t-4a5284847d2a7cb78ff598ebec9e614e720f81a76b14b8124187ff22e793483b3</citedby><cites>FETCH-LOGICAL-c296t-4a5284847d2a7cb78ff598ebec9e614e720f81a76b14b8124187ff22e793483b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Zhou, Yang</creatorcontrib><creatorcontrib>Shao, Xiangming</creatorcontrib><creatorcontrib>Han, Yuwang</creatorcontrib><creatorcontrib>Zhang, Hongman</creatorcontrib><title>Detection of procalcitonin (PCT) using the double antibody sandwich method based on fluorescence resonance energy transfer between upconversion nanoparticles and quantum dots</title><title>Analytical methods</title><description>A nanosensor is designed using a double antibody sandwich method based on fluorescence resonance energy transfer (FRET) between NaYF
4
(Yb, Er) upconversion nanoparticles (UCNPs) and CdTe quantum dots (QDs) for the determination of procalcitonin (PCT). The UCNPs (donor) and CdTe QDs (acceptor) were modified separately with two PCT monoclonal antibodies, and they were bound together through a specific immune reaction
via
the addition of PCT antigen. The fluorescence intensity of UCNPs was quenched because of the resonant energy transferred between upconversion nanoparticles and quantum dots. Under the optimal conditions, the fluorescence quenching efficiency showed a linear response to the PCT concentration with values ranging from 0.1 to 10 ng mL
−1
and a detection limit of 0.25 ng mL
−1
. This method can be easily extended to the double antibody sandwich assay with potential applications in
in vitro
diagnostics (IVD).</description><subject>Cadmium tellurides</subject><subject>Energy transfer</subject><subject>Erbium</subject><subject>Fluorescence</subject><subject>Fluorescence resonance energy transfer</subject><subject>Monoclonal antibodies</subject><subject>Nanoparticles</subject><subject>Procalcitonin</subject><subject>Quantum dots</subject><subject>Resonance</subject><subject>Upconversion</subject><subject>Ytterbium</subject><issn>1759-9660</issn><issn>1759-9679</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNpFkctKAzEUhgdRsF42PkHAjQqjSWaay7LUegFBF7pwNSSZEztlmrRJxtKX8hlNUXR1vsXP-Q7nL4ozgq8JruTNVEzeMc402ytGhI9lKRmX-3_M8GFxFOMCYyYrRkbF1y0kMKnzDnmLVsEb1Zsuedc5dPEyfb1EQ-zcB0pzQK0fdA9IudRp325RVK7ddGaOlpDmvkVaRWhR3mT7wQeIBpwBlME7tSNwED62KAXlooWANKQNgEPDynj3CSHurshRv1IhdaaHmF0tWg_ZOCyzPsWT4sCqPsLp7zwu3u5mr9OH8un5_nE6eSoNlSyVtRpTUYuat1Rxo7mwdiwFaDASGKmBU2wFUZxpUmtBaE0Et5ZS4LKqRaWr4-L8Z2_-yHqAmJqFH4LLyoZiggVllFU5dfWTMsHHGMA2q9AtVdg2BDe7Ppr_PqpvA6CBcA</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Zhou, Yang</creator><creator>Shao, Xiangming</creator><creator>Han, Yuwang</creator><creator>Zhang, Hongman</creator><general>Royal Society of Chemistry</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>L7M</scope><scope>P64</scope></search><sort><creationdate>20180101</creationdate><title>Detection of procalcitonin (PCT) using the double antibody sandwich method based on fluorescence resonance energy transfer between upconversion nanoparticles and quantum dots</title><author>Zhou, Yang ; Shao, Xiangming ; Han, Yuwang ; Zhang, Hongman</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c296t-4a5284847d2a7cb78ff598ebec9e614e720f81a76b14b8124187ff22e793483b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Cadmium tellurides</topic><topic>Energy transfer</topic><topic>Erbium</topic><topic>Fluorescence</topic><topic>Fluorescence resonance energy transfer</topic><topic>Monoclonal antibodies</topic><topic>Nanoparticles</topic><topic>Procalcitonin</topic><topic>Quantum dots</topic><topic>Resonance</topic><topic>Upconversion</topic><topic>Ytterbium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhou, Yang</creatorcontrib><creatorcontrib>Shao, Xiangming</creatorcontrib><creatorcontrib>Han, Yuwang</creatorcontrib><creatorcontrib>Zhang, Hongman</creatorcontrib><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Analytical methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Yang</au><au>Shao, Xiangming</au><au>Han, Yuwang</au><au>Zhang, Hongman</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of procalcitonin (PCT) using the double antibody sandwich method based on fluorescence resonance energy transfer between upconversion nanoparticles and quantum dots</atitle><jtitle>Analytical methods</jtitle><date>2018-01-01</date><risdate>2018</risdate><volume>10</volume><issue>9</issue><spage>1015</spage><epage>1022</epage><pages>1015-1022</pages><issn>1759-9660</issn><eissn>1759-9679</eissn><abstract>A nanosensor is designed using a double antibody sandwich method based on fluorescence resonance energy transfer (FRET) between NaYF
4
(Yb, Er) upconversion nanoparticles (UCNPs) and CdTe quantum dots (QDs) for the determination of procalcitonin (PCT). The UCNPs (donor) and CdTe QDs (acceptor) were modified separately with two PCT monoclonal antibodies, and they were bound together through a specific immune reaction
via
the addition of PCT antigen. The fluorescence intensity of UCNPs was quenched because of the resonant energy transferred between upconversion nanoparticles and quantum dots. Under the optimal conditions, the fluorescence quenching efficiency showed a linear response to the PCT concentration with values ranging from 0.1 to 10 ng mL
−1
and a detection limit of 0.25 ng mL
−1
. This method can be easily extended to the double antibody sandwich assay with potential applications in
in vitro
diagnostics (IVD).</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><doi>10.1039/C8AY00039E</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1759-9660 |
| ispartof | Analytical methods, 2018-01, Vol.10 (9), p.1015-1022 |
| issn | 1759-9660 1759-9679 |
| language | eng |
| recordid | cdi_proquest_journals_2010826263 |
| source | Royal Society Of Chemistry Journals 2008- |
| subjects | Cadmium tellurides Energy transfer Erbium Fluorescence Fluorescence resonance energy transfer Monoclonal antibodies Nanoparticles Procalcitonin Quantum dots Resonance Upconversion Ytterbium |
| title | Detection of procalcitonin (PCT) using the double antibody sandwich method based on fluorescence resonance energy transfer between upconversion nanoparticles and quantum dots |
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