In vitro and in vivo imaging and tracking of intestinal organoids from human induced pluripotent stem cells

Human intestinal organoids (hIOs) derived from human pluripotent stem cells (hPSCs) have immense potential as a source of intestines. Therefore, an efficient system is needed for visualizing the stage of intestinal differentiation and further identifying hIOs derived from hPSCs. Here, 2 fluorescent...

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Veröffentlicht in:	The FASEB journal 2018-01, Vol.32 (1), p.111-122
Hauptverfasser:	Jung, Kwang Bo, Lee, Hana, Son, Ye Seul, Lee, Ji Hye, Cho, Hyun‐Soo, Lee, Mi‐Ok, Oh, Jung‐Hwa, Lee, Jaemin, Kim, Seokho, Jung, Cho‐Rok, Kim, Janghwan, Son, Mi‐Young
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biosensing Techniques Biosensors Cell Differentiation - genetics Cell Line Computer Systems Differentiation Fluorescence fluorescence imaging Gene expression Genes, Reporter Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Heterografts Homeobox Homeodomain Proteins - genetics Homeodomain Proteins - metabolism human intestine Humans Imaging Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - metabolism Induced Pluripotent Stem Cells - transplantation Intestinal Mucosa - metabolism Intestine Intestine, Small - cytology Intestine, Small - metabolism Intestines - cytology ISX KLF5 Krueppel-like factor Kruppel-Like Transcription Factors - genetics Kruppel-Like Transcription Factors - metabolism Localization Luminescent Proteins - genetics Luminescent Proteins - metabolism Mice Mice, Inbred NOD Mice, Knockout Mice, SCID Noggin protein Organoids Organoids - cytology Organoids - metabolism Pluripotency Real-Time Polymerase Chain Reaction Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Red Fluorescent Protein Reporter gene reporter system Small intestine Stem cells Tracking Transcription Factors - genetics Transcription Factors - metabolism Transplantation
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