DNA damage and circulating cell free DNA in greenhouse workers exposed to pesticides
Pesticides widely used in agriculture and other applications have been linked to cancer and other diseases through several potential mechanisms. The goals of this study were to assess DNA damage in lymphocytes by the cytokinesis‐block micronucleus assay (CBMN), and to measure circulating cell free D...
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| Veröffentlicht in: | Environmental and molecular mutagenesis 2018-03, Vol.59 (2), p.161-169 |
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| creator | Çayir, Akin Coskun, Mahmut Coskun, Munevver Cobanoglu, Hayal Bonassi, S. |
| description | Pesticides widely used in agriculture and other applications have been linked to cancer and other diseases through several potential mechanisms. The goals of this study were to assess DNA damage in lymphocytes by the cytokinesis‐block micronucleus assay (CBMN), and to measure circulating cell free DNA (ccf‐DNA) in the blood of pesticide‐exposed greenhouse workers and matched controls living in the same area. CBMN was applied to peripheral blood lymphocyte samples taken at different times (spring and autumn) for each individual. We measured plasma ccf‐DNA levels using a Qubit® fluorometer. The results indicated that the MNL, BNMN, and NBUDs frequencies of pesticide‐exposed individuals were significantly higher than non‐exposed individuals. Apart from MNL, BNMN and CBPI, a season‐related effect was found for the NPB and NBUD frequencies. With MNL and BNMN as the dependent variables, multiple regression analysis showed that age and gender affected MN formation. The ccf‐DNA level in the pesticide‐exposed group was significantly higher than the control group. There was no seasonal variation regarding the free DNA amount. Ccf‐DNA in males was found to be higher than females. The MNL, BNMN, NPB, and CBPI did not correlate with the ccf‐DNA amount. It can be concluded that pesticide exposure can modulate DNA integrity via different mechanisms. Also, elevated levels of ccf‐DNA could be recommended as a biomarker of pesticide exposure. Environ. Mol. Mutagen. 59:161–169, 2018. © 2017 Wiley Periodicals, Inc. |
| doi_str_mv | 10.1002/em.22148 |
| format | Article |
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The goals of this study were to assess DNA damage in lymphocytes by the cytokinesis‐block micronucleus assay (CBMN), and to measure circulating cell free DNA (ccf‐DNA) in the blood of pesticide‐exposed greenhouse workers and matched controls living in the same area. CBMN was applied to peripheral blood lymphocyte samples taken at different times (spring and autumn) for each individual. We measured plasma ccf‐DNA levels using a Qubit® fluorometer. The results indicated that the MNL, BNMN, and NBUDs frequencies of pesticide‐exposed individuals were significantly higher than non‐exposed individuals. Apart from MNL, BNMN and CBPI, a season‐related effect was found for the NPB and NBUD frequencies. With MNL and BNMN as the dependent variables, multiple regression analysis showed that age and gender affected MN formation. The ccf‐DNA level in the pesticide‐exposed group was significantly higher than the control group. There was no seasonal variation regarding the free DNA amount. Ccf‐DNA in males was found to be higher than females. The MNL, BNMN, NPB, and CBPI did not correlate with the ccf‐DNA amount. It can be concluded that pesticide exposure can modulate DNA integrity via different mechanisms. Also, elevated levels of ccf‐DNA could be recommended as a biomarker of pesticide exposure. Environ. Mol. Mutagen. 59:161–169, 2018. © 2017 Wiley Periodicals, Inc.</description><identifier>ISSN: 0893-6692</identifier><identifier>EISSN: 1098-2280</identifier><identifier>DOI: 10.1002/em.22148</identifier><identifier>PMID: 29130594</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Agrochemicals ; Biomarkers ; Blood circulation ; Cancer ; circulating cell free DNA ; Cytokinesis ; Damage assessment ; Deoxyribonucleic acid ; Dependent variables ; DNA ; DNA damage ; Exposure ; Females ; greenhouse workers ; Lymphocytes ; Males ; micronucleus ; Multiple regression analysis ; nucleoplasmic bridge ; Occupational exposure ; Peripheral blood ; Pesticides ; Regression analysis ; Seasonal variations</subject><ispartof>Environmental and molecular mutagenesis, 2018-03, Vol.59 (2), p.161-169</ispartof><rights>2017 Wiley Periodicals, Inc.</rights><rights>2018 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3498-870d64f310b7fc7f8de7feeb1ced15a1b23ae8c06309fbe51607c343bb919313</citedby><cites>FETCH-LOGICAL-c3498-870d64f310b7fc7f8de7feeb1ced15a1b23ae8c06309fbe51607c343bb919313</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fem.22148$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fem.22148$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29130594$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Çayir, Akin</creatorcontrib><creatorcontrib>Coskun, Mahmut</creatorcontrib><creatorcontrib>Coskun, Munevver</creatorcontrib><creatorcontrib>Cobanoglu, Hayal</creatorcontrib><creatorcontrib>Bonassi, S.</creatorcontrib><title>DNA damage and circulating cell free DNA in greenhouse workers exposed to pesticides</title><title>Environmental and molecular mutagenesis</title><addtitle>Environ Mol Mutagen</addtitle><description>Pesticides widely used in agriculture and other applications have been linked to cancer and other diseases through several potential mechanisms. The goals of this study were to assess DNA damage in lymphocytes by the cytokinesis‐block micronucleus assay (CBMN), and to measure circulating cell free DNA (ccf‐DNA) in the blood of pesticide‐exposed greenhouse workers and matched controls living in the same area. CBMN was applied to peripheral blood lymphocyte samples taken at different times (spring and autumn) for each individual. We measured plasma ccf‐DNA levels using a Qubit® fluorometer. The results indicated that the MNL, BNMN, and NBUDs frequencies of pesticide‐exposed individuals were significantly higher than non‐exposed individuals. Apart from MNL, BNMN and CBPI, a season‐related effect was found for the NPB and NBUD frequencies. With MNL and BNMN as the dependent variables, multiple regression analysis showed that age and gender affected MN formation. The ccf‐DNA level in the pesticide‐exposed group was significantly higher than the control group. There was no seasonal variation regarding the free DNA amount. Ccf‐DNA in males was found to be higher than females. The MNL, BNMN, NPB, and CBPI did not correlate with the ccf‐DNA amount. It can be concluded that pesticide exposure can modulate DNA integrity via different mechanisms. Also, elevated levels of ccf‐DNA could be recommended as a biomarker of pesticide exposure. Environ. Mol. Mutagen. 59:161–169, 2018. © 2017 Wiley Periodicals, Inc.</description><subject>Agrochemicals</subject><subject>Biomarkers</subject><subject>Blood circulation</subject><subject>Cancer</subject><subject>circulating cell free DNA</subject><subject>Cytokinesis</subject><subject>Damage assessment</subject><subject>Deoxyribonucleic acid</subject><subject>Dependent variables</subject><subject>DNA</subject><subject>DNA damage</subject><subject>Exposure</subject><subject>Females</subject><subject>greenhouse workers</subject><subject>Lymphocytes</subject><subject>Males</subject><subject>micronucleus</subject><subject>Multiple regression analysis</subject><subject>nucleoplasmic bridge</subject><subject>Occupational exposure</subject><subject>Peripheral blood</subject><subject>Pesticides</subject><subject>Regression analysis</subject><subject>Seasonal variations</subject><issn>0893-6692</issn><issn>1098-2280</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kE1PwzAMhiMEYmMg8QtQJC5cOpyka5vjNMaHNOCye5Qm7ujox0hWjf17MgrcONmHx4_tl5BLBmMGwG-xHnPO4uyIDBnILOI8g2MyhEyKKEkkH5Az79cAjMWSn5IBl0zARMZDsrx7mVKra71CqhtLTelMV-lt2ayowaqihUOkB6hs6Cr0zVvbeaS71r2j8xQ_N61HS7ct3aDflqa06M_JSaErjxc_dUSW9_Pl7DFavD48zaaLyIg4XJmlYJO4EAzytDBpkVlMC8ScGbRsolnOhcbMQCJAFjlOWAJpmBR5LpkUTIzIda_duPajC9vVuu1cEzYqHn4VacwhDtRNTxnXeu-wUBtX1trtFQN1SE9hrb7TC-jVj7DLa7R_4G9cAYh6YFdWuP9XpObPvfALmsF3Bg</recordid><startdate>201803</startdate><enddate>201803</enddate><creator>Çayir, Akin</creator><creator>Coskun, Mahmut</creator><creator>Coskun, Munevver</creator><creator>Cobanoglu, Hayal</creator><creator>Bonassi, S.</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope></search><sort><creationdate>201803</creationdate><title>DNA damage and circulating cell free DNA in greenhouse workers exposed to pesticides</title><author>Çayir, Akin ; Coskun, Mahmut ; Coskun, Munevver ; Cobanoglu, Hayal ; Bonassi, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3498-870d64f310b7fc7f8de7feeb1ced15a1b23ae8c06309fbe51607c343bb919313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Agrochemicals</topic><topic>Biomarkers</topic><topic>Blood circulation</topic><topic>Cancer</topic><topic>circulating cell free DNA</topic><topic>Cytokinesis</topic><topic>Damage assessment</topic><topic>Deoxyribonucleic acid</topic><topic>Dependent variables</topic><topic>DNA</topic><topic>DNA damage</topic><topic>Exposure</topic><topic>Females</topic><topic>greenhouse workers</topic><topic>Lymphocytes</topic><topic>Males</topic><topic>micronucleus</topic><topic>Multiple regression analysis</topic><topic>nucleoplasmic bridge</topic><topic>Occupational exposure</topic><topic>Peripheral blood</topic><topic>Pesticides</topic><topic>Regression analysis</topic><topic>Seasonal variations</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Çayir, Akin</creatorcontrib><creatorcontrib>Coskun, Mahmut</creatorcontrib><creatorcontrib>Coskun, Munevver</creatorcontrib><creatorcontrib>Cobanoglu, Hayal</creatorcontrib><creatorcontrib>Bonassi, S.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Environmental and molecular mutagenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Çayir, Akin</au><au>Coskun, Mahmut</au><au>Coskun, Munevver</au><au>Cobanoglu, Hayal</au><au>Bonassi, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA damage and circulating cell free DNA in greenhouse workers exposed to pesticides</atitle><jtitle>Environmental and molecular mutagenesis</jtitle><addtitle>Environ Mol Mutagen</addtitle><date>2018-03</date><risdate>2018</risdate><volume>59</volume><issue>2</issue><spage>161</spage><epage>169</epage><pages>161-169</pages><issn>0893-6692</issn><eissn>1098-2280</eissn><abstract>Pesticides widely used in agriculture and other applications have been linked to cancer and other diseases through several potential mechanisms. 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Ccf‐DNA in males was found to be higher than females. The MNL, BNMN, NPB, and CBPI did not correlate with the ccf‐DNA amount. It can be concluded that pesticide exposure can modulate DNA integrity via different mechanisms. Also, elevated levels of ccf‐DNA could be recommended as a biomarker of pesticide exposure. Environ. Mol. Mutagen. 59:161–169, 2018. © 2017 Wiley Periodicals, Inc.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>29130594</pmid><doi>10.1002/em.22148</doi><tpages>9</tpages></addata></record> |
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| subjects | Agrochemicals Biomarkers Blood circulation Cancer circulating cell free DNA Cytokinesis Damage assessment Deoxyribonucleic acid Dependent variables DNA DNA damage Exposure Females greenhouse workers Lymphocytes Males micronucleus Multiple regression analysis nucleoplasmic bridge Occupational exposure Peripheral blood Pesticides Regression analysis Seasonal variations |
| title | DNA damage and circulating cell free DNA in greenhouse workers exposed to pesticides |
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