Identification and characterization of Gluconacetobacter diazotrophicus mutants defective in the solubilization of phosphorus and zinc
Gluconacetobacter diazotrophicus is a plant-growth-promoting bacterium, which is able to colonize sugarcane and other plant species of economic importance. The potentially beneficial effects promoted by this bacterium on plants are nitrogen-fixation, production of phythormones, action against pathog...
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| Veröffentlicht in: | Archives of microbiology 2009-05, Vol.191 (5), p.477-483 |
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| creator | Intorne, Aline C de Oliveira, Marcos Vinicius V Lima, Mariana L da Silva, Juliana F Olivares, Fábio L de Souza Filho, Gonçalo Apolinário |
| description | Gluconacetobacter diazotrophicus is a plant-growth-promoting bacterium, which is able to colonize sugarcane and other plant species of economic importance. The potentially beneficial effects promoted by this bacterium on plants are nitrogen-fixation, production of phythormones, action against pathogens and mineral nutrient solubilization. In this study, the molecular mechanisms associated with phosphorus and zinc solubilization were analyzed. A transposon mutant library was constructed and screened to select for mutants defective for phosphorous [Ca₅(PO₄)₃OH] and zinc (ZnO) solubilization. A total of five mutants were identified in each screen. Both screenings, performed independently, allowed to select the same mutants. The interrupted gene in each mutant was identified by sequencing and the results demonstrate that the production of gluconic acid is a required pathway for solubilization of such nutrients in G. diazotrophicus. |
| doi_str_mv | 10.1007/s00203-009-0472-0 |
| format | Article |
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The potentially beneficial effects promoted by this bacterium on plants are nitrogen-fixation, production of phythormones, action against pathogens and mineral nutrient solubilization. In this study, the molecular mechanisms associated with phosphorus and zinc solubilization were analyzed. A transposon mutant library was constructed and screened to select for mutants defective for phosphorous [Ca₅(PO₄)₃OH] and zinc (ZnO) solubilization. A total of five mutants were identified in each screen. Both screenings, performed independently, allowed to select the same mutants. The interrupted gene in each mutant was identified by sequencing and the results demonstrate that the production of gluconic acid is a required pathway for solubilization of such nutrients in G. diazotrophicus.</description><identifier>ISSN: 0302-8933</identifier><identifier>EISSN: 1432-072X</identifier><identifier>DOI: 10.1007/s00203-009-0472-0</identifier><identifier>PMID: 19340412</identifier><identifier>CODEN: AMICCW</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Bacteriology ; Biochemistry ; Biological and medical sciences ; Biomedical and Life Sciences ; Biotechnology ; Cell Biology ; DNA Transposable Elements ; Ecology ; Economic importance ; Flowers & plants ; Fundamental and applied biological sciences. Psychology ; Gene Deletion ; Gluconacetobacter - genetics ; Gluconacetobacter - metabolism ; Gluconates - metabolism ; Libraries ; Life Sciences ; Metabolic Networks and Pathways - genetics ; Microbial Ecology ; Microbiology ; Miscellaneous ; Mutagenesis, Insertional ; Mutants ; Mutation ; Nitrogen ; Nitrogen fixation ; Nutrients ; Pathogens ; Phosphorus ; Phosphorus - metabolism ; Plant species ; Saccharum - microbiology ; Short Communication ; Sugarcane ; Zinc ; Zinc - metabolism</subject><ispartof>Archives of microbiology, 2009-05, Vol.191 (5), p.477-483</ispartof><rights>Springer-Verlag 2009</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c489t-c01917fafa72225681a9bf63c7311b564663a1175b8c691842d57f11e3df27873</citedby><cites>FETCH-LOGICAL-c489t-c01917fafa72225681a9bf63c7311b564663a1175b8c691842d57f11e3df27873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00203-009-0472-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00203-009-0472-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21415815$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19340412$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Intorne, Aline C</creatorcontrib><creatorcontrib>de Oliveira, Marcos Vinicius V</creatorcontrib><creatorcontrib>Lima, Mariana L</creatorcontrib><creatorcontrib>da Silva, Juliana F</creatorcontrib><creatorcontrib>Olivares, Fábio L</creatorcontrib><creatorcontrib>de Souza Filho, Gonçalo Apolinário</creatorcontrib><title>Identification and characterization of Gluconacetobacter diazotrophicus mutants defective in the solubilization of phosphorus and zinc</title><title>Archives of microbiology</title><addtitle>Arch Microbiol</addtitle><addtitle>Arch Microbiol</addtitle><description>Gluconacetobacter diazotrophicus is a plant-growth-promoting bacterium, which is able to colonize sugarcane and other plant species of economic importance. The potentially beneficial effects promoted by this bacterium on plants are nitrogen-fixation, production of phythormones, action against pathogens and mineral nutrient solubilization. In this study, the molecular mechanisms associated with phosphorus and zinc solubilization were analyzed. A transposon mutant library was constructed and screened to select for mutants defective for phosphorous [Ca₅(PO₄)₃OH] and zinc (ZnO) solubilization. A total of five mutants were identified in each screen. Both screenings, performed independently, allowed to select the same mutants. The interrupted gene in each mutant was identified by sequencing and the results demonstrate that the production of gluconic acid is a required pathway for solubilization of such nutrients in G. diazotrophicus.</description><subject>Bacteriology</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cell Biology</subject><subject>DNA Transposable Elements</subject><subject>Ecology</subject><subject>Economic importance</subject><subject>Flowers & plants</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>Gene Deletion</topic><topic>Gluconacetobacter - genetics</topic><topic>Gluconacetobacter - metabolism</topic><topic>Gluconates - metabolism</topic><topic>Libraries</topic><topic>Life Sciences</topic><topic>Metabolic Networks and Pathways - genetics</topic><topic>Microbial Ecology</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Mutagenesis, Insertional</topic><topic>Mutants</topic><topic>Mutation</topic><topic>Nitrogen</topic><topic>Nitrogen fixation</topic><topic>Nutrients</topic><topic>Pathogens</topic><topic>Phosphorus</topic><topic>Phosphorus - metabolism</topic><topic>Plant species</topic><topic>Saccharum - microbiology</topic><topic>Short Communication</topic><topic>Sugarcane</topic><topic>Zinc</topic><topic>Zinc - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Intorne, Aline C</creatorcontrib><creatorcontrib>de Oliveira, Marcos Vinicius V</creatorcontrib><creatorcontrib>Lima, Mariana L</creatorcontrib><creatorcontrib>da Silva, Juliana F</creatorcontrib><creatorcontrib>Olivares, Fábio L</creatorcontrib><creatorcontrib>de Souza Filho, Gonçalo Apolinário</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>Archives of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Intorne, Aline C</au><au>de Oliveira, Marcos Vinicius V</au><au>Lima, Mariana L</au><au>da Silva, Juliana F</au><au>Olivares, Fábio L</au><au>de Souza Filho, Gonçalo Apolinário</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and characterization of Gluconacetobacter diazotrophicus mutants defective in the solubilization of phosphorus and zinc</atitle><jtitle>Archives of microbiology</jtitle><stitle>Arch Microbiol</stitle><addtitle>Arch Microbiol</addtitle><date>2009-05-01</date><risdate>2009</risdate><volume>191</volume><issue>5</issue><spage>477</spage><epage>483</epage><pages>477-483</pages><issn>0302-8933</issn><eissn>1432-072X</eissn><coden>AMICCW</coden><abstract>Gluconacetobacter diazotrophicus is a plant-growth-promoting bacterium, which is able to colonize sugarcane and other plant species of economic importance. 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| ispartof | Archives of microbiology, 2009-05, Vol.191 (5), p.477-483 |
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| subjects | Bacteriology Biochemistry Biological and medical sciences Biomedical and Life Sciences Biotechnology Cell Biology DNA Transposable Elements Ecology Economic importance Flowers & plants Fundamental and applied biological sciences. Psychology Gene Deletion Gluconacetobacter - genetics Gluconacetobacter - metabolism Gluconates - metabolism Libraries Life Sciences Metabolic Networks and Pathways - genetics Microbial Ecology Microbiology Miscellaneous Mutagenesis, Insertional Mutants Mutation Nitrogen Nitrogen fixation Nutrients Pathogens Phosphorus Phosphorus - metabolism Plant species Saccharum - microbiology Short Communication Sugarcane Zinc Zinc - metabolism |
| title | Identification and characterization of Gluconacetobacter diazotrophicus mutants defective in the solubilization of phosphorus and zinc |
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