Safety study of intravitreal and suprachoroidal Laponite clay in rabbit eyes
Purpose To study the safety and biocompatibility of Laponite clay (LAP) within an intravitreal and suprachoroidal administration in rabbit eyes. Methods Thirty-two New Zealand albino rabbits were divided into two experimental groups to test intravitreal (IVT group) and suprachoroidal (SCS group) adm...
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| Veröffentlicht in: | Graefe's archive for clinical and experimental ophthalmology 2018-03, Vol.256 (3), p.535-546 |
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| container_title | Graefe's archive for clinical and experimental ophthalmology |
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| creator | Prieto, Esther Vispe, Eugenio De Martino, Alba Idoipe, Miriam Rodrigo, María J. Garcia-Martin, Elena Fraile, José M. Polo-Llorens, Vicente Mayoral, José A. |
| description | Purpose
To study the safety and biocompatibility of Laponite clay (LAP) within an intravitreal and suprachoroidal administration in rabbit eyes.
Methods
Thirty-two New Zealand albino rabbits were divided into two experimental groups to test intravitreal (IVT group) and suprachoroidal (SCS group) administration of a 100-μl and 50-μl Laponite suspension respectively. Following injection, the eyes were monitored by ocular tonometry, slit-lamp eye examination and indirect ophthalmoscopy, at 24 h, 1, 4, 12, and 14 weeks post administration. Histological examination was also performed to determine whether any ocular pathological change had occurred. Throughout the study, LAP presence in vitreous was estimated by complexometric titration with ethylenediaminetetraacetic acid (EDTA), taking advantage of the Laponite high content of magnesium ions.
Results
Neither significant differences in the intraocular pressure, nor relevant ocular complications were found in the two experimental groups after LAP administration. The histology of the retina remained unchanged. LAP presence in vitreous could be indirectly confirmed by complexometric titration until 14 weeks post administration in eyes of IVT group.
Conclusion
Laponite could be considered as a vehicle for potential clinical use in ocular drug administration, due to its proven ocular biocompatibility and its transparency in gel state. |
| doi_str_mv | 10.1007/s00417-017-3893-5 |
| format | Article |
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To study the safety and biocompatibility of Laponite clay (LAP) within an intravitreal and suprachoroidal administration in rabbit eyes.
Methods
Thirty-two New Zealand albino rabbits were divided into two experimental groups to test intravitreal (IVT group) and suprachoroidal (SCS group) administration of a 100-μl and 50-μl Laponite suspension respectively. Following injection, the eyes were monitored by ocular tonometry, slit-lamp eye examination and indirect ophthalmoscopy, at 24 h, 1, 4, 12, and 14 weeks post administration. Histological examination was also performed to determine whether any ocular pathological change had occurred. Throughout the study, LAP presence in vitreous was estimated by complexometric titration with ethylenediaminetetraacetic acid (EDTA), taking advantage of the Laponite high content of magnesium ions.
Results
Neither significant differences in the intraocular pressure, nor relevant ocular complications were found in the two experimental groups after LAP administration. The histology of the retina remained unchanged. LAP presence in vitreous could be indirectly confirmed by complexometric titration until 14 weeks post administration in eyes of IVT group.
Conclusion
Laponite could be considered as a vehicle for potential clinical use in ocular drug administration, due to its proven ocular biocompatibility and its transparency in gel state.</description><identifier>ISSN: 0721-832X</identifier><identifier>EISSN: 1435-702X</identifier><identifier>DOI: 10.1007/s00417-017-3893-5</identifier><identifier>PMID: 29353344</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acetic acid ; Basic Science ; Biocompatibility ; Eye ; Histology ; Magnesium ; Medicine ; Medicine & Public Health ; Ophthalmology ; Retina ; Titration</subject><ispartof>Graefe's archive for clinical and experimental ophthalmology, 2018-03, Vol.256 (3), p.535-546</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2018</rights><rights>Graefe's Archive for Clinical and Experimental Ophthalmology is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-3ac1482985e1317dc15fd6d2fda2972cd31addb32118143ffa0abcb69e381463</citedby><cites>FETCH-LOGICAL-c372t-3ac1482985e1317dc15fd6d2fda2972cd31addb32118143ffa0abcb69e381463</cites><orcidid>0000-0002-4820-0293</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00417-017-3893-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00417-017-3893-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29353344$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Prieto, Esther</creatorcontrib><creatorcontrib>Vispe, Eugenio</creatorcontrib><creatorcontrib>De Martino, Alba</creatorcontrib><creatorcontrib>Idoipe, Miriam</creatorcontrib><creatorcontrib>Rodrigo, María J.</creatorcontrib><creatorcontrib>Garcia-Martin, Elena</creatorcontrib><creatorcontrib>Fraile, José M.</creatorcontrib><creatorcontrib>Polo-Llorens, Vicente</creatorcontrib><creatorcontrib>Mayoral, José A.</creatorcontrib><title>Safety study of intravitreal and suprachoroidal Laponite clay in rabbit eyes</title><title>Graefe's archive for clinical and experimental ophthalmology</title><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><description>Purpose
To study the safety and biocompatibility of Laponite clay (LAP) within an intravitreal and suprachoroidal administration in rabbit eyes.
Methods
Thirty-two New Zealand albino rabbits were divided into two experimental groups to test intravitreal (IVT group) and suprachoroidal (SCS group) administration of a 100-μl and 50-μl Laponite suspension respectively. Following injection, the eyes were monitored by ocular tonometry, slit-lamp eye examination and indirect ophthalmoscopy, at 24 h, 1, 4, 12, and 14 weeks post administration. Histological examination was also performed to determine whether any ocular pathological change had occurred. Throughout the study, LAP presence in vitreous was estimated by complexometric titration with ethylenediaminetetraacetic acid (EDTA), taking advantage of the Laponite high content of magnesium ions.
Results
Neither significant differences in the intraocular pressure, nor relevant ocular complications were found in the two experimental groups after LAP administration. The histology of the retina remained unchanged. LAP presence in vitreous could be indirectly confirmed by complexometric titration until 14 weeks post administration in eyes of IVT group.
Conclusion
Laponite could be considered as a vehicle for potential clinical use in ocular drug administration, due to its proven ocular biocompatibility and its transparency in gel state.</description><subject>Acetic acid</subject><subject>Basic Science</subject><subject>Biocompatibility</subject><subject>Eye</subject><subject>Histology</subject><subject>Magnesium</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Ophthalmology</subject><subject>Retina</subject><subject>Titration</subject><issn>0721-832X</issn><issn>1435-702X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp1kE1LxDAQhoMo7rr6A7xIwHM1k2m37VHELyh4cA97C2k-tMtuW5NU6L83S1fx4iEMyTzzDnkIuQR2A4zlt56xFPKExYNFiUl2ROaQYpbkjK-PyZzlHJIC-XpGzrzfsIhjBqdkxkvMENN0Tqo3aU0YqQ-DHmlnadMGJ7-a4IzcUtlq6ofeSfXRua7R8amSfdc2wVC1lWOkqZN13QRqRuPPyYmVW28uDnVBVo8Pq_vnpHp9erm_qxKFOQ8JSgVpwcsiM4CQawWZ1UvNrZa8zLnSCFLrGjlAEb9jrWSyVvWyNBjvS1yQ6ym2d93nYHwQm25wbdwooCyRpwyhjBRMlHKd985Y0btmJ90ogIm9PjHpE1Gf2OsTWZy5OiQP9c7o34kfXxHgE-Bjq3037s_qf1O_AQucesc</recordid><startdate>20180301</startdate><enddate>20180301</enddate><creator>Prieto, Esther</creator><creator>Vispe, Eugenio</creator><creator>De Martino, Alba</creator><creator>Idoipe, Miriam</creator><creator>Rodrigo, María J.</creator><creator>Garcia-Martin, Elena</creator><creator>Fraile, José M.</creator><creator>Polo-Llorens, Vicente</creator><creator>Mayoral, José A.</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><orcidid>https://orcid.org/0000-0002-4820-0293</orcidid></search><sort><creationdate>20180301</creationdate><title>Safety study of intravitreal and suprachoroidal Laponite clay in rabbit eyes</title><author>Prieto, Esther ; Vispe, Eugenio ; De Martino, Alba ; Idoipe, Miriam ; Rodrigo, María J. ; Garcia-Martin, Elena ; Fraile, José M. ; Polo-Llorens, Vicente ; Mayoral, José A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-3ac1482985e1317dc15fd6d2fda2972cd31addb32118143ffa0abcb69e381463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Acetic acid</topic><topic>Basic Science</topic><topic>Biocompatibility</topic><topic>Eye</topic><topic>Histology</topic><topic>Magnesium</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Ophthalmology</topic><topic>Retina</topic><topic>Titration</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Prieto, Esther</creatorcontrib><creatorcontrib>Vispe, Eugenio</creatorcontrib><creatorcontrib>De Martino, Alba</creatorcontrib><creatorcontrib>Idoipe, Miriam</creatorcontrib><creatorcontrib>Rodrigo, María J.</creatorcontrib><creatorcontrib>Garcia-Martin, Elena</creatorcontrib><creatorcontrib>Fraile, José M.</creatorcontrib><creatorcontrib>Polo-Llorens, Vicente</creatorcontrib><creatorcontrib>Mayoral, José A.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Graefe's archive for clinical and experimental ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Prieto, Esther</au><au>Vispe, Eugenio</au><au>De Martino, Alba</au><au>Idoipe, Miriam</au><au>Rodrigo, María J.</au><au>Garcia-Martin, Elena</au><au>Fraile, José M.</au><au>Polo-Llorens, Vicente</au><au>Mayoral, José A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Safety study of intravitreal and suprachoroidal Laponite clay in rabbit eyes</atitle><jtitle>Graefe's archive for clinical and experimental ophthalmology</jtitle><stitle>Graefes Arch Clin Exp Ophthalmol</stitle><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><date>2018-03-01</date><risdate>2018</risdate><volume>256</volume><issue>3</issue><spage>535</spage><epage>546</epage><pages>535-546</pages><issn>0721-832X</issn><eissn>1435-702X</eissn><abstract>Purpose
To study the safety and biocompatibility of Laponite clay (LAP) within an intravitreal and suprachoroidal administration in rabbit eyes.
Methods
Thirty-two New Zealand albino rabbits were divided into two experimental groups to test intravitreal (IVT group) and suprachoroidal (SCS group) administration of a 100-μl and 50-μl Laponite suspension respectively. Following injection, the eyes were monitored by ocular tonometry, slit-lamp eye examination and indirect ophthalmoscopy, at 24 h, 1, 4, 12, and 14 weeks post administration. Histological examination was also performed to determine whether any ocular pathological change had occurred. Throughout the study, LAP presence in vitreous was estimated by complexometric titration with ethylenediaminetetraacetic acid (EDTA), taking advantage of the Laponite high content of magnesium ions.
Results
Neither significant differences in the intraocular pressure, nor relevant ocular complications were found in the two experimental groups after LAP administration. The histology of the retina remained unchanged. LAP presence in vitreous could be indirectly confirmed by complexometric titration until 14 weeks post administration in eyes of IVT group.
Conclusion
Laponite could be considered as a vehicle for potential clinical use in ocular drug administration, due to its proven ocular biocompatibility and its transparency in gel state.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>29353344</pmid><doi>10.1007/s00417-017-3893-5</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-4820-0293</orcidid></addata></record> |
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| ispartof | Graefe's archive for clinical and experimental ophthalmology, 2018-03, Vol.256 (3), p.535-546 |
| issn | 0721-832X 1435-702X |
| language | eng |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | Acetic acid Basic Science Biocompatibility Eye Histology Magnesium Medicine Medicine & Public Health Ophthalmology Retina Titration |
| title | Safety study of intravitreal and suprachoroidal Laponite clay in rabbit eyes |
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