IMA chromatography of a cell extract: Effect of loading pH on protein purification

Immobilized metal ion affinity chromatography has shown promise for isolating desired proteins from host cell extracts, based on differential affinities for chelated metal ions. This article focuses on modeling the effect of loading pH on the purity and binding capacity of target proteins, during th...

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Veröffentlicht in:	AIChE journal 1997-12, Vol.43 (12), p.3232-3240
Hauptverfasser:	Patwardhan, Anshuman V., Ataai, Mohammad M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Others Various methods and equipments
Online-Zugang:	Volltext
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