Purification, Characterization of Amylase from Indigenously Isolated Aureobasidium pullulans Cau 19 and Its Bioconjugates with Gold Nanoparticles

The amylase from Aureobasidium pullulans Cau 19 was purified by ammonium sulfate precipitation and Sephadex G-100 chromatography with a 9.25-fold increase in specific activity as compared to crude enzyme. Km and turn over values of the enzyme were 6.25 mg/mL and 5.0 × 10 2 /min, respectively. Effect...

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Veröffentlicht in:	Applied biochemistry and biotechnology 2018-02, Vol.184 (2), p.644-658
Hauptverfasser:	Mulay, Y.R., Deopurkar, R.L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Ammonium Ammonium sulfate Amylases Amylases - chemistry Amylases - isolation & purification Ascomycota - enzymology Aureobasidium pullulans Biochemistry Biotechnology Calcium Chemistry Chemistry and Materials Science Cobalt Conjugation Copper Deactivation Enzymatic activity Enzyme activity Enzymes Enzymes, Immobilized - chemistry Fourier transforms Fungal Proteins - chemistry Fungal Proteins - isolation & purification Gold Gold - chemistry High temperature Inactivation Infrared spectroscopy Iron Manganese Mercury Mercury (metal) Metal Nanoparticles - chemistry Nanoparticles pH effects Purification Sulfates
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