EMS-induced mutation of an endoplasmic reticulum oleate desaturase gene (FAD2-2) results in elevated oleic acid content in rapeseed (Brassica napus L.)
The development of rapeseed cultivars ( Brassica napus L.) with high oleic acid and low linolenic acid is highly desirable for food and industrial applications. In this study, the Korean rapeseed cultivar Tamla was used for ethyl methanesulfonate (EMS)-induced mutagenesis and seed oils were screened...
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| Veröffentlicht in: | Euphytica 2018-02, Vol.214 (2), p.1-12, Article 28 |
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| creator | Lee, Yong-Hwa Park, Won Kim, Kwang-Soo Jang, Young-Seok Lee, Ji-Eun Cha, Young-Lok Moon, Youn-Ho Song, Yeon-Sang Lee, Kyeongbo |
| description | The development of rapeseed cultivars (
Brassica napus
L.) with high oleic acid and low linolenic acid is highly desirable for food and industrial applications. In this study, the Korean rapeseed cultivar Tamla was used for ethyl methanesulfonate (EMS)-induced mutagenesis and seed oils were screened up to generation M
7
for high oleate mutants. Two mutant populations (M
7
) with an average of approximately 76% oleic acid content were isolated. Yield components between two mutant populations and untreated Tamla plants were not substantially different, although the mutants in the vegetative stage were slightly smaller in size than Tamla. Genomic analyses of six fatty acid desaturase (four
FAD2
and two
FAD6
) genes revealed that the elevated oleic acid content in the mutants is the result of single gene mutations. Changes in DNA sequence were observed in two genes out of six fatty acid desaturase (four
FAD2
and two
FAD6
).
FAD2
-
2
exhibited a 2-bp deletion in the upstream region of the gene in the two mutants, resulting in a severely truncated polypeptide (57 aa instead of 469 aa), while six point mutations in the other gene did not result in changes in the amino acid sequence. Based on these results, FAD2-2, an endoplasmic reticulum (ER) oleic acid desaturase, is affected in the mutants, resulting in a ~ 7% increase in oleic acid content in comparison to untreated Tamla plants. The induced mutants could be utilized for the development of high oleic oil rapeseed varieties and for regulatory studies of lipid metabolism in seed oils. |
| doi_str_mv | 10.1007/s10681-017-2106-y |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1992794243</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1992794243</sourcerecordid><originalsourceid>FETCH-LOGICAL-c316t-dffa967b32849eeb8b1ce8e53f41eea3b885998ff1b3a2d47a054d8f6ac4a1983</originalsourceid><addsrcrecordid>eNp1kLuOFDEQRS0EEsPCB5BZItkNvPjRDztc9gFIgwiA2Kq2y6te9bgbP5DmS_hdPBoCEqIqqc49JV1C3gp-LTgf32fBBy0YFyOTbWXHZ2Qn-lGxng_8OdlxLjomlRpeklc5P3HOzdjzHfl9_-Ubm6OvDj091AJlXiNdA4VIMfp1WyAfZkcTltnVpR7ouiAUpB4zlJogI33EiPTy4eZOMnnVyFyXkuncBAv-aqw_ZZoD3OypW2PBWE7nBBtmbOfLD82TZwc0wlYz3V9fvSYvAiwZ3_ydF-THw_33209s__Xj59ubPXNKDIX5EMAM46Sk7gzipCfhUGOvQicQQU1a98boEMSkQPpuBN53XocBXAfCaHVB3p29W1p_VszFPq01xfbSCmPkaDrZqUaJM-XSmnPCYLc0HyAdreD21L89929b__bUvz22jDxncmPjI6Z_zP8N_QFDgooV</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1992794243</pqid></control><display><type>article</type><title>EMS-induced mutation of an endoplasmic reticulum oleate desaturase gene (FAD2-2) results in elevated oleic acid content in rapeseed (Brassica napus L.)</title><source>Springer Nature - Complete Springer Journals</source><creator>Lee, Yong-Hwa ; Park, Won ; Kim, Kwang-Soo ; Jang, Young-Seok ; Lee, Ji-Eun ; Cha, Young-Lok ; Moon, Youn-Ho ; Song, Yeon-Sang ; Lee, Kyeongbo</creator><creatorcontrib>Lee, Yong-Hwa ; Park, Won ; Kim, Kwang-Soo ; Jang, Young-Seok ; Lee, Ji-Eun ; Cha, Young-Lok ; Moon, Youn-Ho ; Song, Yeon-Sang ; Lee, Kyeongbo</creatorcontrib><description>The development of rapeseed cultivars (
Brassica napus
L.) with high oleic acid and low linolenic acid is highly desirable for food and industrial applications. In this study, the Korean rapeseed cultivar Tamla was used for ethyl methanesulfonate (EMS)-induced mutagenesis and seed oils were screened up to generation M
7
for high oleate mutants. Two mutant populations (M
7
) with an average of approximately 76% oleic acid content were isolated. Yield components between two mutant populations and untreated Tamla plants were not substantially different, although the mutants in the vegetative stage were slightly smaller in size than Tamla. Genomic analyses of six fatty acid desaturase (four
FAD2
and two
FAD6
) genes revealed that the elevated oleic acid content in the mutants is the result of single gene mutations. Changes in DNA sequence were observed in two genes out of six fatty acid desaturase (four
FAD2
and two
FAD6
).
FAD2
-
2
exhibited a 2-bp deletion in the upstream region of the gene in the two mutants, resulting in a severely truncated polypeptide (57 aa instead of 469 aa), while six point mutations in the other gene did not result in changes in the amino acid sequence. Based on these results, FAD2-2, an endoplasmic reticulum (ER) oleic acid desaturase, is affected in the mutants, resulting in a ~ 7% increase in oleic acid content in comparison to untreated Tamla plants. The induced mutants could be utilized for the development of high oleic oil rapeseed varieties and for regulatory studies of lipid metabolism in seed oils.</description><identifier>ISSN: 0014-2336</identifier><identifier>EISSN: 1573-5060</identifier><identifier>DOI: 10.1007/s10681-017-2106-y</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Amino acid sequence ; Amino acids ; Biomedical and Life Sciences ; Biotechnology ; Brassica ; Brassica napus ; Cultivars ; Deoxyribonucleic acid ; Desaturase ; DNA ; Edible oils ; Endoplasmic reticulum ; Ethyl methanesulfonate ; Fatty acids ; Food industry ; Gene deletion ; Genes ; Genomic analysis ; Industrial applications ; Life Sciences ; Linolenic acid ; Lipid metabolism ; Metabolism ; Mutagenesis ; Mutants ; Mutation ; Nucleotide sequence ; Oils & fats ; Oleic acid ; Oleic acid desaturase ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Plants (botany) ; Populations ; Rape plants ; Rapeseed ; Seeds</subject><ispartof>Euphytica, 2018-02, Vol.214 (2), p.1-12, Article 28</ispartof><rights>Springer Science+Business Media B.V., part of Springer Nature 2018</rights><rights>Euphytica is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-dffa967b32849eeb8b1ce8e53f41eea3b885998ff1b3a2d47a054d8f6ac4a1983</citedby><cites>FETCH-LOGICAL-c316t-dffa967b32849eeb8b1ce8e53f41eea3b885998ff1b3a2d47a054d8f6ac4a1983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10681-017-2106-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10681-017-2106-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51298</link.rule.ids></links><search><creatorcontrib>Lee, Yong-Hwa</creatorcontrib><creatorcontrib>Park, Won</creatorcontrib><creatorcontrib>Kim, Kwang-Soo</creatorcontrib><creatorcontrib>Jang, Young-Seok</creatorcontrib><creatorcontrib>Lee, Ji-Eun</creatorcontrib><creatorcontrib>Cha, Young-Lok</creatorcontrib><creatorcontrib>Moon, Youn-Ho</creatorcontrib><creatorcontrib>Song, Yeon-Sang</creatorcontrib><creatorcontrib>Lee, Kyeongbo</creatorcontrib><title>EMS-induced mutation of an endoplasmic reticulum oleate desaturase gene (FAD2-2) results in elevated oleic acid content in rapeseed (Brassica napus L.)</title><title>Euphytica</title><addtitle>Euphytica</addtitle><description>The development of rapeseed cultivars (
Brassica napus
L.) with high oleic acid and low linolenic acid is highly desirable for food and industrial applications. In this study, the Korean rapeseed cultivar Tamla was used for ethyl methanesulfonate (EMS)-induced mutagenesis and seed oils were screened up to generation M
7
for high oleate mutants. Two mutant populations (M
7
) with an average of approximately 76% oleic acid content were isolated. Yield components between two mutant populations and untreated Tamla plants were not substantially different, although the mutants in the vegetative stage were slightly smaller in size than Tamla. Genomic analyses of six fatty acid desaturase (four
FAD2
and two
FAD6
) genes revealed that the elevated oleic acid content in the mutants is the result of single gene mutations. Changes in DNA sequence were observed in two genes out of six fatty acid desaturase (four
FAD2
and two
FAD6
).
FAD2
-
2
exhibited a 2-bp deletion in the upstream region of the gene in the two mutants, resulting in a severely truncated polypeptide (57 aa instead of 469 aa), while six point mutations in the other gene did not result in changes in the amino acid sequence. Based on these results, FAD2-2, an endoplasmic reticulum (ER) oleic acid desaturase, is affected in the mutants, resulting in a ~ 7% increase in oleic acid content in comparison to untreated Tamla plants. The induced mutants could be utilized for the development of high oleic oil rapeseed varieties and for regulatory studies of lipid metabolism in seed oils.</description><subject>Amino acid sequence</subject><subject>Amino acids</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Brassica</subject><subject>Brassica napus</subject><subject>Cultivars</subject><subject>Deoxyribonucleic acid</subject><subject>Desaturase</subject><subject>DNA</subject><subject>Edible oils</subject><subject>Endoplasmic reticulum</subject><subject>Ethyl methanesulfonate</subject><subject>Fatty acids</subject><subject>Food industry</subject><subject>Gene deletion</subject><subject>Genes</subject><subject>Genomic analysis</subject><subject>Industrial applications</subject><subject>Life Sciences</subject><subject>Linolenic acid</subject><subject>Lipid metabolism</subject><subject>Metabolism</subject><subject>Mutagenesis</subject><subject>Mutants</subject><subject>Mutation</subject><subject>Nucleotide sequence</subject><subject>Oils & fats</subject><subject>Oleic acid</subject><subject>Oleic acid desaturase</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Plants (botany)</subject><subject>Populations</subject><subject>Rape plants</subject><subject>Rapeseed</subject><subject>Seeds</subject><issn>0014-2336</issn><issn>1573-5060</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kLuOFDEQRS0EEsPCB5BZItkNvPjRDztc9gFIgwiA2Kq2y6te9bgbP5DmS_hdPBoCEqIqqc49JV1C3gp-LTgf32fBBy0YFyOTbWXHZ2Qn-lGxng_8OdlxLjomlRpeklc5P3HOzdjzHfl9_-Ubm6OvDj091AJlXiNdA4VIMfp1WyAfZkcTltnVpR7ouiAUpB4zlJogI33EiPTy4eZOMnnVyFyXkuncBAv-aqw_ZZoD3OypW2PBWE7nBBtmbOfLD82TZwc0wlYz3V9fvSYvAiwZ3_ydF-THw_33209s__Xj59ubPXNKDIX5EMAM46Sk7gzipCfhUGOvQicQQU1a98boEMSkQPpuBN53XocBXAfCaHVB3p29W1p_VszFPq01xfbSCmPkaDrZqUaJM-XSmnPCYLc0HyAdreD21L89929b__bUvz22jDxncmPjI6Z_zP8N_QFDgooV</recordid><startdate>20180201</startdate><enddate>20180201</enddate><creator>Lee, Yong-Hwa</creator><creator>Park, Won</creator><creator>Kim, Kwang-Soo</creator><creator>Jang, Young-Seok</creator><creator>Lee, Ji-Eun</creator><creator>Cha, Young-Lok</creator><creator>Moon, Youn-Ho</creator><creator>Song, Yeon-Sang</creator><creator>Lee, Kyeongbo</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SN</scope><scope>7SS</scope><scope>7T7</scope><scope>7TM</scope><scope>7X2</scope><scope>7XB</scope><scope>88I</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>M0K</scope><scope>M2P</scope><scope>M7N</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>RC3</scope></search><sort><creationdate>20180201</creationdate><title>EMS-induced mutation of an endoplasmic reticulum oleate desaturase gene (FAD2-2) results in elevated oleic acid content in rapeseed (Brassica napus L.)</title><author>Lee, Yong-Hwa ; Park, Won ; Kim, Kwang-Soo ; Jang, Young-Seok ; Lee, Ji-Eun ; Cha, Young-Lok ; Moon, Youn-Ho ; Song, Yeon-Sang ; Lee, Kyeongbo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-dffa967b32849eeb8b1ce8e53f41eea3b885998ff1b3a2d47a054d8f6ac4a1983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Amino acid sequence</topic><topic>Amino acids</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Brassica</topic><topic>Brassica napus</topic><topic>Cultivars</topic><topic>Deoxyribonucleic acid</topic><topic>Desaturase</topic><topic>DNA</topic><topic>Edible oils</topic><topic>Endoplasmic reticulum</topic><topic>Ethyl methanesulfonate</topic><topic>Fatty acids</topic><topic>Food industry</topic><topic>Gene deletion</topic><topic>Genes</topic><topic>Genomic analysis</topic><topic>Industrial applications</topic><topic>Life Sciences</topic><topic>Linolenic acid</topic><topic>Lipid metabolism</topic><topic>Metabolism</topic><topic>Mutagenesis</topic><topic>Mutants</topic><topic>Mutation</topic><topic>Nucleotide sequence</topic><topic>Oils & fats</topic><topic>Oleic acid</topic><topic>Oleic acid desaturase</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Plants (botany)</topic><topic>Populations</topic><topic>Rape plants</topic><topic>Rapeseed</topic><topic>Seeds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Yong-Hwa</creatorcontrib><creatorcontrib>Park, Won</creatorcontrib><creatorcontrib>Kim, Kwang-Soo</creatorcontrib><creatorcontrib>Jang, Young-Seok</creatorcontrib><creatorcontrib>Lee, Ji-Eun</creatorcontrib><creatorcontrib>Cha, Young-Lok</creatorcontrib><creatorcontrib>Moon, Youn-Ho</creatorcontrib><creatorcontrib>Song, Yeon-Sang</creatorcontrib><creatorcontrib>Lee, Kyeongbo</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Science Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Agricultural Science Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><jtitle>Euphytica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Yong-Hwa</au><au>Park, Won</au><au>Kim, Kwang-Soo</au><au>Jang, Young-Seok</au><au>Lee, Ji-Eun</au><au>Cha, Young-Lok</au><au>Moon, Youn-Ho</au><au>Song, Yeon-Sang</au><au>Lee, Kyeongbo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>EMS-induced mutation of an endoplasmic reticulum oleate desaturase gene (FAD2-2) results in elevated oleic acid content in rapeseed (Brassica napus L.)</atitle><jtitle>Euphytica</jtitle><stitle>Euphytica</stitle><date>2018-02-01</date><risdate>2018</risdate><volume>214</volume><issue>2</issue><spage>1</spage><epage>12</epage><pages>1-12</pages><artnum>28</artnum><issn>0014-2336</issn><eissn>1573-5060</eissn><abstract>The development of rapeseed cultivars (
Brassica napus
L.) with high oleic acid and low linolenic acid is highly desirable for food and industrial applications. In this study, the Korean rapeseed cultivar Tamla was used for ethyl methanesulfonate (EMS)-induced mutagenesis and seed oils were screened up to generation M
7
for high oleate mutants. Two mutant populations (M
7
) with an average of approximately 76% oleic acid content were isolated. Yield components between two mutant populations and untreated Tamla plants were not substantially different, although the mutants in the vegetative stage were slightly smaller in size than Tamla. Genomic analyses of six fatty acid desaturase (four
FAD2
and two
FAD6
) genes revealed that the elevated oleic acid content in the mutants is the result of single gene mutations. Changes in DNA sequence were observed in two genes out of six fatty acid desaturase (four
FAD2
and two
FAD6
).
FAD2
-
2
exhibited a 2-bp deletion in the upstream region of the gene in the two mutants, resulting in a severely truncated polypeptide (57 aa instead of 469 aa), while six point mutations in the other gene did not result in changes in the amino acid sequence. Based on these results, FAD2-2, an endoplasmic reticulum (ER) oleic acid desaturase, is affected in the mutants, resulting in a ~ 7% increase in oleic acid content in comparison to untreated Tamla plants. The induced mutants could be utilized for the development of high oleic oil rapeseed varieties and for regulatory studies of lipid metabolism in seed oils.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s10681-017-2106-y</doi><tpages>12</tpages></addata></record> |
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| ispartof | Euphytica, 2018-02, Vol.214 (2), p.1-12, Article 28 |
| issn | 0014-2336 1573-5060 |
| language | eng |
| recordid | cdi_proquest_journals_1992794243 |
| source | Springer Nature - Complete Springer Journals |
| subjects | Amino acid sequence Amino acids Biomedical and Life Sciences Biotechnology Brassica Brassica napus Cultivars Deoxyribonucleic acid Desaturase DNA Edible oils Endoplasmic reticulum Ethyl methanesulfonate Fatty acids Food industry Gene deletion Genes Genomic analysis Industrial applications Life Sciences Linolenic acid Lipid metabolism Metabolism Mutagenesis Mutants Mutation Nucleotide sequence Oils & fats Oleic acid Oleic acid desaturase Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Plants (botany) Populations Rape plants Rapeseed Seeds |
| title | EMS-induced mutation of an endoplasmic reticulum oleate desaturase gene (FAD2-2) results in elevated oleic acid content in rapeseed (Brassica napus L.) |
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