Anti-oxidant, hemolysis inhibition, and collagen-stimulating activities of a new hexapeptide derived from Arthrospira (Spirulina) platensis
In this study, the whole protein of Arthrospira ( Spirulina ) platensis was extracted and hydrolyzed with trypsin. Screening process for new peptides was driven by 2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging activity assay, and a hexapeptide with sequence of GMCCSR...
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| Veröffentlicht in: | Journal of applied phycology 2018-06, Vol.30 (3), p.1655-1665 |
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| creator | Zeng, Qiaohui Fan, Xiaodan Zheng, Qiuping Wang, Jingjing Zhang, Xuewu |
| description | In this study, the whole protein of
Arthrospira
(
Spirulina
)
platensis
was extracted and hydrolyzed with trypsin. Screening process for new peptides was driven by 2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging activity assay, and a hexapeptide with sequence of GMCCSR was identified. Its anti-oxidant activity was measured based on ABTS, 1,1’-diphenyl-2-picrylhydrazyl (DPPH), and ferric reducing ability of plasma (FRAP) assays. Hemolysis inhibition on human erythrocytes and collagen-stimulating activities on human skin fibroblasts (HSFs) were investigated. Results showed that its radical scavenging effect could compete with ascorbic acid. Intracellular assays revealed the reduction of reactive oxygen species (ROS) and malondialdehyde (MDA) contents and the increase in activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in erythrocytes pretreated with hexapeptide, demonstrating that the peptide could protect erythrocytes from lipid peroxidation and protein oxidation. Moreover, results from HSFs showed that it could promote proliferation and collagen production of HSFs pro-damaged by UVB. These results suggest the potential of the hexapeptide in food and pharmaceutical industries. |
| doi_str_mv | 10.1007/s10811-017-1378-x |
| format | Article |
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Arthrospira
(
Spirulina
)
platensis
was extracted and hydrolyzed with trypsin. Screening process for new peptides was driven by 2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging activity assay, and a hexapeptide with sequence of GMCCSR was identified. Its anti-oxidant activity was measured based on ABTS, 1,1’-diphenyl-2-picrylhydrazyl (DPPH), and ferric reducing ability of plasma (FRAP) assays. Hemolysis inhibition on human erythrocytes and collagen-stimulating activities on human skin fibroblasts (HSFs) were investigated. Results showed that its radical scavenging effect could compete with ascorbic acid. Intracellular assays revealed the reduction of reactive oxygen species (ROS) and malondialdehyde (MDA) contents and the increase in activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in erythrocytes pretreated with hexapeptide, demonstrating that the peptide could protect erythrocytes from lipid peroxidation and protein oxidation. Moreover, results from HSFs showed that it could promote proliferation and collagen production of HSFs pro-damaged by UVB. These results suggest the potential of the hexapeptide in food and pharmaceutical industries.</description><identifier>ISSN: 0921-8971</identifier><identifier>EISSN: 1573-5176</identifier><identifier>DOI: 10.1007/s10811-017-1378-x</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Antioxidants ; Arthrospira ; Ascorbic acid ; Assaying ; Biomedical and Life Sciences ; Catalase ; Collagen ; Ecology ; Erythrocytes ; Fibroblasts ; Food industry ; Freshwater & Marine Ecology ; Glutathione ; Glutathione peroxidase ; Haemolysis ; Hemolysis ; Industry ; Life Sciences ; Lipid peroxidation ; Lipids ; Malondialdehyde ; Oxidation ; Oxidizing agents ; Peptides ; Peroxidase ; Peroxidation ; Pharmaceutical industry ; Plant Physiology ; Plant Sciences ; Proliferation ; Proteins ; Reactive oxygen species ; Scavenging ; Skin ; Spirulina ; Sulfonic acid ; Superoxide dismutase ; Trypsin</subject><ispartof>Journal of applied phycology, 2018-06, Vol.30 (3), p.1655-1665</ispartof><rights>Springer Science+Business Media B.V., part of Springer Nature 2018</rights><rights>Journal of Applied Phycology is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-b7bf39b0e02461fe8902ca06b917d7a6e057e12e12270c5739a96bc4e38502de3</citedby><cites>FETCH-LOGICAL-c316t-b7bf39b0e02461fe8902ca06b917d7a6e057e12e12270c5739a96bc4e38502de3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10811-017-1378-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10811-017-1378-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51298</link.rule.ids></links><search><creatorcontrib>Zeng, Qiaohui</creatorcontrib><creatorcontrib>Fan, Xiaodan</creatorcontrib><creatorcontrib>Zheng, Qiuping</creatorcontrib><creatorcontrib>Wang, Jingjing</creatorcontrib><creatorcontrib>Zhang, Xuewu</creatorcontrib><title>Anti-oxidant, hemolysis inhibition, and collagen-stimulating activities of a new hexapeptide derived from Arthrospira (Spirulina) platensis</title><title>Journal of applied phycology</title><addtitle>J Appl Phycol</addtitle><description>In this study, the whole protein of
Arthrospira
(
Spirulina
)
platensis
was extracted and hydrolyzed with trypsin. Screening process for new peptides was driven by 2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging activity assay, and a hexapeptide with sequence of GMCCSR was identified. Its anti-oxidant activity was measured based on ABTS, 1,1’-diphenyl-2-picrylhydrazyl (DPPH), and ferric reducing ability of plasma (FRAP) assays. Hemolysis inhibition on human erythrocytes and collagen-stimulating activities on human skin fibroblasts (HSFs) were investigated. Results showed that its radical scavenging effect could compete with ascorbic acid. Intracellular assays revealed the reduction of reactive oxygen species (ROS) and malondialdehyde (MDA) contents and the increase in activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in erythrocytes pretreated with hexapeptide, demonstrating that the peptide could protect erythrocytes from lipid peroxidation and protein oxidation. Moreover, results from HSFs showed that it could promote proliferation and collagen production of HSFs pro-damaged by UVB. These results suggest the potential of the hexapeptide in food and pharmaceutical industries.</description><subject>Antioxidants</subject><subject>Arthrospira</subject><subject>Ascorbic acid</subject><subject>Assaying</subject><subject>Biomedical and Life Sciences</subject><subject>Catalase</subject><subject>Collagen</subject><subject>Ecology</subject><subject>Erythrocytes</subject><subject>Fibroblasts</subject><subject>Food industry</subject><subject>Freshwater & Marine Ecology</subject><subject>Glutathione</subject><subject>Glutathione peroxidase</subject><subject>Haemolysis</subject><subject>Hemolysis</subject><subject>Industry</subject><subject>Life Sciences</subject><subject>Lipid peroxidation</subject><subject>Lipids</subject><subject>Malondialdehyde</subject><subject>Oxidation</subject><subject>Oxidizing agents</subject><subject>Peptides</subject><subject>Peroxidase</subject><subject>Peroxidation</subject><subject>Pharmaceutical industry</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Proliferation</subject><subject>Proteins</subject><subject>Reactive oxygen species</subject><subject>Scavenging</subject><subject>Skin</subject><subject>Spirulina</subject><subject>Sulfonic acid</subject><subject>Superoxide dismutase</subject><subject>Trypsin</subject><issn>0921-8971</issn><issn>1573-5176</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kM9qGzEQxkVoIG7SB-hNkEsLUTOjjVerowlJWzD0kOQstLuztsJa2kiyaz9DXroy7qGXwsB3-f4MP8Y-I3xDAHWbEBpEAagEVqoR-zM2w7mqxBxV_YHNQEsUjVZ4wT6m9AoAusFmxt4XPjsR9q63Pt_wNW3CeEgucefXrnXZBX_Dre95F8bRrsiLlN1mO9rs_IrbLrtdMVHiYeCWe_pdKvZ2oim7nnhP0e2o50MMG76IeR1Dmly0_MtTke3ovP3Kp1JGvmxesfPBjok-_dVL9vL48Hz_Qyx_ff95v1iKrsI6i1a1Q6VbIJB3NQ7UaJCdhbrVqHpla4K5IpTlpIKuMNBW1213R1UzB9lTdcmuT71TDG9bStm8hm30ZdKg1lI1WlayuPDk6srTKdJgpug2Nh4MgjkyNyfmpjA3R-ZmXzLylEnF61cU_2n-b-gPl6aHdw</recordid><startdate>20180601</startdate><enddate>20180601</enddate><creator>Zeng, Qiaohui</creator><creator>Fan, Xiaodan</creator><creator>Zheng, Qiuping</creator><creator>Wang, Jingjing</creator><creator>Zhang, Xuewu</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TN</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>GNUQQ</scope><scope>H95</scope><scope>HCIFZ</scope><scope>L.G</scope><scope>LK8</scope><scope>M0K</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20180601</creationdate><title>Anti-oxidant, hemolysis inhibition, and collagen-stimulating activities of a new hexapeptide derived from Arthrospira (Spirulina) platensis</title><author>Zeng, Qiaohui ; Fan, Xiaodan ; Zheng, Qiuping ; Wang, Jingjing ; Zhang, Xuewu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-b7bf39b0e02461fe8902ca06b917d7a6e057e12e12270c5739a96bc4e38502de3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Antioxidants</topic><topic>Arthrospira</topic><topic>Ascorbic acid</topic><topic>Assaying</topic><topic>Biomedical and Life Sciences</topic><topic>Catalase</topic><topic>Collagen</topic><topic>Ecology</topic><topic>Erythrocytes</topic><topic>Fibroblasts</topic><topic>Food industry</topic><topic>Freshwater & Marine Ecology</topic><topic>Glutathione</topic><topic>Glutathione peroxidase</topic><topic>Haemolysis</topic><topic>Hemolysis</topic><topic>Industry</topic><topic>Life Sciences</topic><topic>Lipid peroxidation</topic><topic>Lipids</topic><topic>Malondialdehyde</topic><topic>Oxidation</topic><topic>Oxidizing agents</topic><topic>Peptides</topic><topic>Peroxidase</topic><topic>Peroxidation</topic><topic>Pharmaceutical industry</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Proliferation</topic><topic>Proteins</topic><topic>Reactive oxygen species</topic><topic>Scavenging</topic><topic>Skin</topic><topic>Spirulina</topic><topic>Sulfonic acid</topic><topic>Superoxide dismutase</topic><topic>Trypsin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zeng, Qiaohui</creatorcontrib><creatorcontrib>Fan, Xiaodan</creatorcontrib><creatorcontrib>Zheng, Qiuping</creatorcontrib><creatorcontrib>Wang, Jingjing</creatorcontrib><creatorcontrib>Zhang, Xuewu</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Oceanic Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>ProQuest Central Student</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>SciTech Premium Collection</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Journal of applied phycology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zeng, Qiaohui</au><au>Fan, Xiaodan</au><au>Zheng, Qiuping</au><au>Wang, Jingjing</au><au>Zhang, Xuewu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-oxidant, hemolysis inhibition, and collagen-stimulating activities of a new hexapeptide derived from Arthrospira (Spirulina) platensis</atitle><jtitle>Journal of applied phycology</jtitle><stitle>J Appl Phycol</stitle><date>2018-06-01</date><risdate>2018</risdate><volume>30</volume><issue>3</issue><spage>1655</spage><epage>1665</epage><pages>1655-1665</pages><issn>0921-8971</issn><eissn>1573-5176</eissn><abstract>In this study, the whole protein of
Arthrospira
(
Spirulina
)
platensis
was extracted and hydrolyzed with trypsin. Screening process for new peptides was driven by 2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging activity assay, and a hexapeptide with sequence of GMCCSR was identified. Its anti-oxidant activity was measured based on ABTS, 1,1’-diphenyl-2-picrylhydrazyl (DPPH), and ferric reducing ability of plasma (FRAP) assays. Hemolysis inhibition on human erythrocytes and collagen-stimulating activities on human skin fibroblasts (HSFs) were investigated. Results showed that its radical scavenging effect could compete with ascorbic acid. Intracellular assays revealed the reduction of reactive oxygen species (ROS) and malondialdehyde (MDA) contents and the increase in activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in erythrocytes pretreated with hexapeptide, demonstrating that the peptide could protect erythrocytes from lipid peroxidation and protein oxidation. Moreover, results from HSFs showed that it could promote proliferation and collagen production of HSFs pro-damaged by UVB. These results suggest the potential of the hexapeptide in food and pharmaceutical industries.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s10811-017-1378-x</doi><tpages>11</tpages></addata></record> |
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| ispartof | Journal of applied phycology, 2018-06, Vol.30 (3), p.1655-1665 |
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| language | eng |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Antioxidants Arthrospira Ascorbic acid Assaying Biomedical and Life Sciences Catalase Collagen Ecology Erythrocytes Fibroblasts Food industry Freshwater & Marine Ecology Glutathione Glutathione peroxidase Haemolysis Hemolysis Industry Life Sciences Lipid peroxidation Lipids Malondialdehyde Oxidation Oxidizing agents Peptides Peroxidase Peroxidation Pharmaceutical industry Plant Physiology Plant Sciences Proliferation Proteins Reactive oxygen species Scavenging Skin Spirulina Sulfonic acid Superoxide dismutase Trypsin |
| title | Anti-oxidant, hemolysis inhibition, and collagen-stimulating activities of a new hexapeptide derived from Arthrospira (Spirulina) platensis |
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