MiR-129-5p Inhibits Proliferation and Invasion of Chondrosarcoma Cells by Regulating SOX4/Wnt/β-Catenin Signaling Pathway
Recently, microRNAs (miRNA) have been identified as novel regulators in Chondrosarcoma (CHS). This study was aimed to identify the roles of miR-129-5p-5p in regulation of SOX4 and Wnt/β-catenin signaling pathway, as well as cell proliferation and apoptosis in chondrosarcomas. Tissue samples were obt...
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| Veröffentlicht in: | Cellular physiology and biochemistry 2017-01, Vol.42 (1), p.242-253 |
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| creator | Zhang, Peng Li, Jifeng Song, Yuze Wang , Xiao |
| description | Recently, microRNAs (miRNA) have been identified as novel regulators in Chondrosarcoma (CHS). This study was aimed to identify the roles of miR-129-5p-5p in regulation of SOX4 and Wnt/β-catenin signaling pathway, as well as cell proliferation and apoptosis in chondrosarcomas.
Tissue samples were obtained from chondrosarcoma patients. Immunohistochemistry, real-time quantitative RT-PCR (RT-qPCR) and western blot analysis were performed to detect the expressions of miR-129-5p and SOX4. Luciferase assay was conducted to confirm that miR-129-5p directly targeted SOX4 mRNA. Manipulations of miR-129-5p and SOX4 expression were achieved through cell transfection. Cell proliferation, migration and apoptosis were evaluated by CCK-8 assay, colony forming assay, wound healing assay and flow cytometry in vitro. For in vivo experiment, the tumor xenograft model was established to evaluate the effects of miR-129-5p and SOX4 on chondrosarcomas.
The expression of miR-129-5p was significantly down-regulated in chondrosarcoma tissues as well as cells in comparison with normal ones, while SOX4 was over-activated. Further studies suggested that miR-129-5p suppressed cell proliferation, migration and promoted apoptosis by inhibiting SOX4 and Wnt/β-catenin pathway.
MiR-129-5p inhibits the Wnt/β-catenin signaling pathway by targeting SOX4 and further suppresses cell proliferation, migration and promotes apoptosis in chondrosarcomas. |
| doi_str_mv | 10.1159/000477323 |
| format | Article |
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Tissue samples were obtained from chondrosarcoma patients. Immunohistochemistry, real-time quantitative RT-PCR (RT-qPCR) and western blot analysis were performed to detect the expressions of miR-129-5p and SOX4. Luciferase assay was conducted to confirm that miR-129-5p directly targeted SOX4 mRNA. Manipulations of miR-129-5p and SOX4 expression were achieved through cell transfection. Cell proliferation, migration and apoptosis were evaluated by CCK-8 assay, colony forming assay, wound healing assay and flow cytometry in vitro. For in vivo experiment, the tumor xenograft model was established to evaluate the effects of miR-129-5p and SOX4 on chondrosarcomas.
The expression of miR-129-5p was significantly down-regulated in chondrosarcoma tissues as well as cells in comparison with normal ones, while SOX4 was over-activated. Further studies suggested that miR-129-5p suppressed cell proliferation, migration and promoted apoptosis by inhibiting SOX4 and Wnt/β-catenin pathway.
MiR-129-5p inhibits the Wnt/β-catenin signaling pathway by targeting SOX4 and further suppresses cell proliferation, migration and promotes apoptosis in chondrosarcomas.</description><identifier>ISSN: 1015-8987</identifier><identifier>EISSN: 1421-9778</identifier><identifier>DOI: 10.1159/000477323</identifier><identifier>PMID: 28535514</identifier><language>eng</language><publisher>Basel, Switzerland: S. Karger AG</publisher><subject>Animals ; Antagomirs - metabolism ; Apoptosis ; beta Catenin - metabolism ; Bone cancer ; Bone Neoplasms - metabolism ; Bone Neoplasms - pathology ; Cell cycle ; Cell growth ; Cell Line, Tumor ; Cell Movement ; Cell Proliferation ; Chemotherapy ; Chondrosarcoma ; Chondrosarcoma - metabolism ; Chondrosarcoma - pathology ; Cytokines ; Down-Regulation ; Epigenetics ; Esophagus ; Female ; Gastric cancer ; Gene expression ; Humans ; Liver cancer ; Lung cancer ; Male ; Melanoma ; Mice ; Mice, SCID ; MicroRNAs ; MicroRNAs - antagonists & inhibitors ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miR-129-5p ; Prostate cancer ; Radiation therapy ; Retracted Paper ; Signal Transduction ; SOX4 ; SOXC Transcription Factors - antagonists & inhibitors ; SOXC Transcription Factors - genetics ; SOXC Transcription Factors - metabolism ; Transcription factors ; Up-Regulation ; Wnt Proteins - metabolism ; Wnt/β-catenin signaling pathway</subject><ispartof>Cellular physiology and biochemistry, 2017-01, Vol.42 (1), p.242-253</ispartof><rights>2017 The Author(s). Published by S. Karger AG, Basel</rights><rights>2017 The Author(s). Published by S. Karger AG, Basel.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-65a5d8f1700c3f936128beec1d10ccdd78976855ec6875a654a40bb52a4981193</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,2096,27612,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28535514$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Peng</creatorcontrib><creatorcontrib>Li, Jifeng</creatorcontrib><creatorcontrib>Song, Yuze</creatorcontrib><creatorcontrib>Wang , Xiao</creatorcontrib><title>MiR-129-5p Inhibits Proliferation and Invasion of Chondrosarcoma Cells by Regulating SOX4/Wnt/β-Catenin Signaling Pathway</title><title>Cellular physiology and biochemistry</title><addtitle>Cell Physiol Biochem</addtitle><description>Recently, microRNAs (miRNA) have been identified as novel regulators in Chondrosarcoma (CHS). This study was aimed to identify the roles of miR-129-5p-5p in regulation of SOX4 and Wnt/β-catenin signaling pathway, as well as cell proliferation and apoptosis in chondrosarcomas.
Tissue samples were obtained from chondrosarcoma patients. Immunohistochemistry, real-time quantitative RT-PCR (RT-qPCR) and western blot analysis were performed to detect the expressions of miR-129-5p and SOX4. Luciferase assay was conducted to confirm that miR-129-5p directly targeted SOX4 mRNA. Manipulations of miR-129-5p and SOX4 expression were achieved through cell transfection. Cell proliferation, migration and apoptosis were evaluated by CCK-8 assay, colony forming assay, wound healing assay and flow cytometry in vitro. For in vivo experiment, the tumor xenograft model was established to evaluate the effects of miR-129-5p and SOX4 on chondrosarcomas.
The expression of miR-129-5p was significantly down-regulated in chondrosarcoma tissues as well as cells in comparison with normal ones, while SOX4 was over-activated. Further studies suggested that miR-129-5p suppressed cell proliferation, migration and promoted apoptosis by inhibiting SOX4 and Wnt/β-catenin pathway.
MiR-129-5p inhibits the Wnt/β-catenin signaling pathway by targeting SOX4 and further suppresses cell proliferation, migration and promotes apoptosis in chondrosarcomas.</description><subject>Animals</subject><subject>Antagomirs - metabolism</subject><subject>Apoptosis</subject><subject>beta Catenin - metabolism</subject><subject>Bone cancer</subject><subject>Bone Neoplasms - metabolism</subject><subject>Bone Neoplasms - pathology</subject><subject>Cell cycle</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement</subject><subject>Cell Proliferation</subject><subject>Chemotherapy</subject><subject>Chondrosarcoma</subject><subject>Chondrosarcoma - metabolism</subject><subject>Chondrosarcoma - pathology</subject><subject>Cytokines</subject><subject>Down-Regulation</subject><subject>Epigenetics</subject><subject>Esophagus</subject><subject>Female</subject><subject>Gastric cancer</subject><subject>Gene expression</subject><subject>Humans</subject><subject>Liver cancer</subject><subject>Lung cancer</subject><subject>Male</subject><subject>Melanoma</subject><subject>Mice</subject><subject>Mice, SCID</subject><subject>MicroRNAs</subject><subject>MicroRNAs - antagonists & inhibitors</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>miR-129-5p</subject><subject>Prostate cancer</subject><subject>Radiation therapy</subject><subject>Retracted Paper</subject><subject>Signal Transduction</subject><subject>SOX4</subject><subject>SOXC Transcription Factors - antagonists & inhibitors</subject><subject>SOXC Transcription Factors - genetics</subject><subject>SOXC Transcription Factors - metabolism</subject><subject>Transcription factors</subject><subject>Up-Regulation</subject><subject>Wnt Proteins - metabolism</subject><subject>Wnt/β-catenin signaling pathway</subject><issn>1015-8987</issn><issn>1421-9778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>M--</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNptkc2O0zAUhSMEYoaBBXuEIrGBRahvHMf2EiJ-Kg2aagYEu-j6J61LGhc7AZXH4kF4JlxaioRY2ffez-da52TZQyDPAZicEUIqzmlJb2XnUJVQSM7F7XQnwAohBT_L7sW4JqnksrybnZWCUcagOs--v3PXBZSyYNt8PqyccmPMF8H3rrMBR-eHHAeTRl8x7gvf5c3KDyb4iEH7DeaN7fuYq11-bZdTn54My_zm6lM1-ziMs58_igZHO7ghv3HLAfv9dIHj6hvu7md3OuyjfXA8L7IPr1-9b94Wl1dv5s2Ly0JXNR2LmiEzogNOiKadpDWUQlmrwQDR2hguJK8FY1bXgjOsWYUVUYqVWEkBIOlFNj_oGo_rdhvcBsOu9eja3w0fli2G0enetlwJi0wIqYSqaNcpIboSKDJidM0tT1pPD1rb4L9MNo7txkWdHMDB-im2IEkJhBOgCX3yD7r2U0gW7ClJBNQUIFHPDpROjsZgu9MHgbT7cNtTuIl9fFSc1MaaE_knzb8rP2NY2nACmsXLg0S7NV2iHv2XOm75BWdEsfY</recordid><startdate>20170101</startdate><enddate>20170101</enddate><creator>Zhang, Peng</creator><creator>Li, Jifeng</creator><creator>Song, Yuze</creator><creator>Wang , Xiao</creator><general>S. Karger AG</general><general>Cell Physiol Biochem Press GmbH & Co KG</general><scope>M--</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>DOA</scope></search><sort><creationdate>20170101</creationdate><title>MiR-129-5p Inhibits Proliferation and Invasion of Chondrosarcoma Cells by Regulating SOX4/Wnt/β-Catenin Signaling Pathway</title><author>Zhang, Peng ; Li, Jifeng ; Song, Yuze ; Wang , Xiao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463t-65a5d8f1700c3f936128beec1d10ccdd78976855ec6875a654a40bb52a4981193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Antagomirs - metabolism</topic><topic>Apoptosis</topic><topic>beta Catenin - metabolism</topic><topic>Bone cancer</topic><topic>Bone Neoplasms - metabolism</topic><topic>Bone Neoplasms - pathology</topic><topic>Cell cycle</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement</topic><topic>Cell Proliferation</topic><topic>Chemotherapy</topic><topic>Chondrosarcoma</topic><topic>Chondrosarcoma - metabolism</topic><topic>Chondrosarcoma - pathology</topic><topic>Cytokines</topic><topic>Down-Regulation</topic><topic>Epigenetics</topic><topic>Esophagus</topic><topic>Female</topic><topic>Gastric cancer</topic><topic>Gene expression</topic><topic>Humans</topic><topic>Liver cancer</topic><topic>Lung cancer</topic><topic>Male</topic><topic>Melanoma</topic><topic>Mice</topic><topic>Mice, SCID</topic><topic>MicroRNAs</topic><topic>MicroRNAs - antagonists & inhibitors</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>miR-129-5p</topic><topic>Prostate cancer</topic><topic>Radiation therapy</topic><topic>Retracted Paper</topic><topic>Signal Transduction</topic><topic>SOX4</topic><topic>SOXC Transcription Factors - antagonists & inhibitors</topic><topic>SOXC Transcription Factors - genetics</topic><topic>SOXC Transcription Factors - metabolism</topic><topic>Transcription factors</topic><topic>Up-Regulation</topic><topic>Wnt Proteins - metabolism</topic><topic>Wnt/β-catenin signaling pathway</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Peng</creatorcontrib><creatorcontrib>Li, Jifeng</creatorcontrib><creatorcontrib>Song, Yuze</creatorcontrib><creatorcontrib>Wang , Xiao</creatorcontrib><collection>Karger Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Cellular physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Peng</au><au>Li, Jifeng</au><au>Song, Yuze</au><au>Wang , Xiao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MiR-129-5p Inhibits Proliferation and Invasion of Chondrosarcoma Cells by Regulating SOX4/Wnt/β-Catenin Signaling Pathway</atitle><jtitle>Cellular physiology and biochemistry</jtitle><addtitle>Cell Physiol Biochem</addtitle><date>2017-01-01</date><risdate>2017</risdate><volume>42</volume><issue>1</issue><spage>242</spage><epage>253</epage><pages>242-253</pages><issn>1015-8987</issn><eissn>1421-9778</eissn><abstract>Recently, microRNAs (miRNA) have been identified as novel regulators in Chondrosarcoma (CHS). This study was aimed to identify the roles of miR-129-5p-5p in regulation of SOX4 and Wnt/β-catenin signaling pathway, as well as cell proliferation and apoptosis in chondrosarcomas.
Tissue samples were obtained from chondrosarcoma patients. Immunohistochemistry, real-time quantitative RT-PCR (RT-qPCR) and western blot analysis were performed to detect the expressions of miR-129-5p and SOX4. Luciferase assay was conducted to confirm that miR-129-5p directly targeted SOX4 mRNA. Manipulations of miR-129-5p and SOX4 expression were achieved through cell transfection. Cell proliferation, migration and apoptosis were evaluated by CCK-8 assay, colony forming assay, wound healing assay and flow cytometry in vitro. For in vivo experiment, the tumor xenograft model was established to evaluate the effects of miR-129-5p and SOX4 on chondrosarcomas.
The expression of miR-129-5p was significantly down-regulated in chondrosarcoma tissues as well as cells in comparison with normal ones, while SOX4 was over-activated. Further studies suggested that miR-129-5p suppressed cell proliferation, migration and promoted apoptosis by inhibiting SOX4 and Wnt/β-catenin pathway.
MiR-129-5p inhibits the Wnt/β-catenin signaling pathway by targeting SOX4 and further suppresses cell proliferation, migration and promotes apoptosis in chondrosarcomas.</abstract><cop>Basel, Switzerland</cop><pub>S. Karger AG</pub><pmid>28535514</pmid><doi>10.1159/000477323</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Antagomirs - metabolism Apoptosis beta Catenin - metabolism Bone cancer Bone Neoplasms - metabolism Bone Neoplasms - pathology Cell cycle Cell growth Cell Line, Tumor Cell Movement Cell Proliferation Chemotherapy Chondrosarcoma Chondrosarcoma - metabolism Chondrosarcoma - pathology Cytokines Down-Regulation Epigenetics Esophagus Female Gastric cancer Gene expression Humans Liver cancer Lung cancer Male Melanoma Mice Mice, SCID MicroRNAs MicroRNAs - antagonists & inhibitors MicroRNAs - genetics MicroRNAs - metabolism miR-129-5p Prostate cancer Radiation therapy Retracted Paper Signal Transduction SOX4 SOXC Transcription Factors - antagonists & inhibitors SOXC Transcription Factors - genetics SOXC Transcription Factors - metabolism Transcription factors Up-Regulation Wnt Proteins - metabolism Wnt/β-catenin signaling pathway |
| title | MiR-129-5p Inhibits Proliferation and Invasion of Chondrosarcoma Cells by Regulating SOX4/Wnt/β-Catenin Signaling Pathway |
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