BIOPSY OF HUMAN PREIMPLANTATION EMBRYOS AND SEXING BY DNA AMPLIFICATION
A single cell was removed, through a hole made in the zona pellucida, from each of 30 human embryos at the 6-10 cell cleavage stage three days after in-vitro fertilisation. A normal proportion of the embryos (37%) developed to the blastocyst stage by day six in culture and 6 hatched from the zona. E...
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| Veröffentlicht in: | The Lancet (British edition) 1989-02, Vol.333 (8634), p.347-349 |
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| container_end_page | 349 |
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| container_issue | 8634 |
| container_start_page | 347 |
| container_title | The Lancet (British edition) |
| container_volume | 333 |
| creator | Handyside, A.H. Penketh, R.J.A. Winston, R.M.L. Pattinson, J.K. Delhanty, J.D.A. Tuddenham, E.G.D. |
| description | A single cell was removed, through a hole made in the zona pellucida, from each of 30 human embryos at the 6-10 cell cleavage stage three days after in-vitro fertilisation. A normal proportion of the embryos (37%) developed to the blastocyst stage by day six in culture and 6 hatched from the zona. Each male embryo was sexed from the DNA by amplification of a repeated sequence specific for the Y chromosome. In 15 embryos with the normal two pronuclei the sex was determined also by in-situ hybridisation with a Y specific probe or fluorescent chromosome staining to detect metaphase Y chromosomes; the results of Y specific amplification were confirmed. This approach may be valuable for couples at risk of transmitting X-linked disease. |
| doi_str_mv | 10.1016/S0140-6736(89)91723-6 |
| format | Article |
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Andrology. Obstetrics</topic><topic>Medical sciences</topic><topic>Sexes</topic><topic>Sterility. 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| fulltext | fulltext |
| identifier | ISSN: 0140-6736 |
| ispartof | The Lancet (British edition), 1989-02, Vol.333 (8634), p.347-349 |
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| language | eng |
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| source | Elsevier ScienceDirect Journals Complete |
| subjects | Biological and medical sciences Birth control Chromosomes Deoxyribonucleic acid DNA Embryos Fetuses Genetics Gynecology. Andrology. Obstetrics Medical sciences Sexes Sterility. Assisted procreation Tests |
| title | BIOPSY OF HUMAN PREIMPLANTATION EMBRYOS AND SEXING BY DNA AMPLIFICATION |
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