Erythrocyte insulin-like growth factor-I binding in younger and older males
OBJECTIVE Insulin‐like growth factor‐I (IGF‐I) levels are lower in older compared with younger subjects. We tested the hypothesis that the reduction in circulating IGF‐I would be accompanied by upregulation in tissue IGF‐I binding in at least some tissues. We tested erythrocyte IGF‐I binding since b...
Gespeichert in:
| Veröffentlicht in: | Clinical endocrinology (Oxford) 1998-03, Vol.48 (3), p.339-345 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 345 |
|---|---|
| container_issue | 3 |
| container_start_page | 339 |
| container_title | Clinical endocrinology (Oxford) |
| container_volume | 48 |
| creator | Moromisato, David Y. Roberts Jr, Charles Brasel, Jo Anne Mohan, Subburaman Cowles, Elizabeth King, Stephen M. Cooper, Dan M. |
| description | OBJECTIVE
Insulin‐like growth factor‐I (IGF‐I) levels are lower in older compared with younger subjects. We tested the hypothesis that the reduction in circulating IGF‐I would be accompanied by upregulation in tissue IGF‐I binding in at least some tissues. We tested erythrocyte IGF‐I binding since blood is an accessible tissue in humans, and there is growing evidence to suggest that erythrocyte IGF‐I binding is influenced by circulating IGF‐I.
DESIGN AND PATIENTS
We compared 9 healthy older males (61–68 years old) with 9 healthy younger males (15–19 years old).
MEASUREMENTS
Standard techniques were used to assay circulating IGF‐I and IGF binding proteins 1–5 (IGFBPs 1–5). Erythrocyte IGF‐I binding was first measured by studies in which native [125I]‐IGF‐I was displaced with unlabelled native IGF‐I. In order to determine a possible role for IGF binding proteins (IGFBP), native [125I]‐IGF‐I was displaced with des‐(1‐3)IGF‐I, which binds with IGF receptors but not IGFBPs.
RESULTS
As expected, circulating IGF‐I was significantly lower in the older compared with the younger subjects. In addition, IGFBP‐3 and 5 were significantly lower, and IGFBP‐4 higher, in older compared with younger subjects. When native [125I]‐IGF‐I was displaced with unlabelled native IGF‐I, the number of IGF‐I binding sites per erythrocyte was higher in the older subjects (43 ± 5 vs. 18 ± 2, older vs. younger, respectively; P |
| doi_str_mv | 10.1046/j.1365-2265.1998.00395.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_198790826</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>28918059</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5205-1e110441a2bc86249bcfd5b332f102b962b73b3a384eb695937d9dc4f02bf9493</originalsourceid><addsrcrecordid>eNqNkF1PwjAUhhujiYj-h8V4u9mPtVsTbwwiIgQTP-Jl03UddIwN2xHYv7cI4dqrnuQ879uTB4AAwQjBmN2XESKMhhgzGiHO0whCwmm0OwO90-Ic9CCBMISMxZfgyrkSQkhTmPTAZGi7dmEb1bU6MLXbVKYOK7PUwdw223YRFFK1jQ3HQWbq3NRzDwVds6nn2gayzoOmyv20kpV21-CikJXTN8e3D76eh5-Dl3D6NhoPHqehohjSEGnkL4-RxJlKGY55poqcZoTgAkGccYazhGREkjTWGeOUkyTnuYoLvyx4zEkf3B5617b52WjXirLZ2Np_KRBPEw5TzDyUHiBlG-esLsTampW0nUBQ7M2JUuwFib0gsTcn_syJnY_eHfulU7IqrKyVcac8RglLGfLYwwHbmkp3_64Xg-HMDz4eHuLGtXp3iku7FCwhCRXfs5GY8PfJlH-8iifyCwfHj2k</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>198790826</pqid></control><display><type>article</type><title>Erythrocyte insulin-like growth factor-I binding in younger and older males</title><source>Wiley Online Library Journals Frontfile Complete</source><creator>Moromisato, David Y. ; Roberts Jr, Charles ; Brasel, Jo Anne ; Mohan, Subburaman ; Cowles, Elizabeth ; King, Stephen M. ; Cooper, Dan M.</creator><creatorcontrib>Moromisato, David Y. ; Roberts Jr, Charles ; Brasel, Jo Anne ; Mohan, Subburaman ; Cowles, Elizabeth ; King, Stephen M. ; Cooper, Dan M.</creatorcontrib><description>OBJECTIVE
Insulin‐like growth factor‐I (IGF‐I) levels are lower in older compared with younger subjects. We tested the hypothesis that the reduction in circulating IGF‐I would be accompanied by upregulation in tissue IGF‐I binding in at least some tissues. We tested erythrocyte IGF‐I binding since blood is an accessible tissue in humans, and there is growing evidence to suggest that erythrocyte IGF‐I binding is influenced by circulating IGF‐I.
DESIGN AND PATIENTS
We compared 9 healthy older males (61–68 years old) with 9 healthy younger males (15–19 years old).
MEASUREMENTS
Standard techniques were used to assay circulating IGF‐I and IGF binding proteins 1–5 (IGFBPs 1–5). Erythrocyte IGF‐I binding was first measured by studies in which native [125I]‐IGF‐I was displaced with unlabelled native IGF‐I. In order to determine a possible role for IGF binding proteins (IGFBP), native [125I]‐IGF‐I was displaced with des‐(1‐3)IGF‐I, which binds with IGF receptors but not IGFBPs.
RESULTS
As expected, circulating IGF‐I was significantly lower in the older compared with the younger subjects. In addition, IGFBP‐3 and 5 were significantly lower, and IGFBP‐4 higher, in older compared with younger subjects. When native [125I]‐IGF‐I was displaced with unlabelled native IGF‐I, the number of IGF‐I binding sites per erythrocyte was higher in the older subjects (43 ± 5 vs. 18 ± 2, older vs. younger, respectively; P < 0.05). In contrast, when native [125I]‐IGF‐I was displaced with des‐(1‐3), IGF‐I binding capacity was not different between the two age groups.
CONCLUSIONS
Erythrocyte IGF binding was increased in older compared with younger subjects. Surprisingly, the mechanism of the increase may not be a simple up regulation of IGF‐I receptors in response to reduced circulating IGF‐I, but possibly by an increase in the levels of as yet unidentified erythrocyte membrane‐associated IGF binding proteins.</description><identifier>ISSN: 0300-0664</identifier><identifier>EISSN: 1365-2265</identifier><identifier>DOI: 10.1046/j.1365-2265.1998.00395.x</identifier><identifier>CODEN: CLECAP</identifier><language>eng</language><publisher>Oxford BSL: Blackwell Science Ltd</publisher><subject>Biological and medical sciences ; Cell receptors ; Cell structures and functions ; Fundamental and applied biological sciences. Psychology ; Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors ; Molecular and cellular biology</subject><ispartof>Clinical endocrinology (Oxford), 1998-03, Vol.48 (3), p.339-345</ispartof><rights>Blackwell Science Ltd, Oxford</rights><rights>1998 INIST-CNRS</rights><rights>Copyright Blackwell Scientific Publications Ltd. Mar 1998</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5205-1e110441a2bc86249bcfd5b332f102b962b73b3a384eb695937d9dc4f02bf9493</citedby><cites>FETCH-LOGICAL-c5205-1e110441a2bc86249bcfd5b332f102b962b73b3a384eb695937d9dc4f02bf9493</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2265.1998.00395.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2265.1998.00395.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2176861$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Moromisato, David Y.</creatorcontrib><creatorcontrib>Roberts Jr, Charles</creatorcontrib><creatorcontrib>Brasel, Jo Anne</creatorcontrib><creatorcontrib>Mohan, Subburaman</creatorcontrib><creatorcontrib>Cowles, Elizabeth</creatorcontrib><creatorcontrib>King, Stephen M.</creatorcontrib><creatorcontrib>Cooper, Dan M.</creatorcontrib><title>Erythrocyte insulin-like growth factor-I binding in younger and older males</title><title>Clinical endocrinology (Oxford)</title><addtitle>Clinical Endocrinology</addtitle><description>OBJECTIVE
Insulin‐like growth factor‐I (IGF‐I) levels are lower in older compared with younger subjects. We tested the hypothesis that the reduction in circulating IGF‐I would be accompanied by upregulation in tissue IGF‐I binding in at least some tissues. We tested erythrocyte IGF‐I binding since blood is an accessible tissue in humans, and there is growing evidence to suggest that erythrocyte IGF‐I binding is influenced by circulating IGF‐I.
DESIGN AND PATIENTS
We compared 9 healthy older males (61–68 years old) with 9 healthy younger males (15–19 years old).
MEASUREMENTS
Standard techniques were used to assay circulating IGF‐I and IGF binding proteins 1–5 (IGFBPs 1–5). Erythrocyte IGF‐I binding was first measured by studies in which native [125I]‐IGF‐I was displaced with unlabelled native IGF‐I. In order to determine a possible role for IGF binding proteins (IGFBP), native [125I]‐IGF‐I was displaced with des‐(1‐3)IGF‐I, which binds with IGF receptors but not IGFBPs.
RESULTS
As expected, circulating IGF‐I was significantly lower in the older compared with the younger subjects. In addition, IGFBP‐3 and 5 were significantly lower, and IGFBP‐4 higher, in older compared with younger subjects. When native [125I]‐IGF‐I was displaced with unlabelled native IGF‐I, the number of IGF‐I binding sites per erythrocyte was higher in the older subjects (43 ± 5 vs. 18 ± 2, older vs. younger, respectively; P < 0.05). In contrast, when native [125I]‐IGF‐I was displaced with des‐(1‐3), IGF‐I binding capacity was not different between the two age groups.
CONCLUSIONS
Erythrocyte IGF binding was increased in older compared with younger subjects. Surprisingly, the mechanism of the increase may not be a simple up regulation of IGF‐I receptors in response to reduced circulating IGF‐I, but possibly by an increase in the levels of as yet unidentified erythrocyte membrane‐associated IGF binding proteins.</description><subject>Biological and medical sciences</subject><subject>Cell receptors</subject><subject>Cell structures and functions</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors</subject><subject>Molecular and cellular biology</subject><issn>0300-0664</issn><issn>1365-2265</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNqNkF1PwjAUhhujiYj-h8V4u9mPtVsTbwwiIgQTP-Jl03UddIwN2xHYv7cI4dqrnuQ879uTB4AAwQjBmN2XESKMhhgzGiHO0whCwmm0OwO90-Ic9CCBMISMxZfgyrkSQkhTmPTAZGi7dmEb1bU6MLXbVKYOK7PUwdw223YRFFK1jQ3HQWbq3NRzDwVds6nn2gayzoOmyv20kpV21-CikJXTN8e3D76eh5-Dl3D6NhoPHqehohjSEGnkL4-RxJlKGY55poqcZoTgAkGccYazhGREkjTWGeOUkyTnuYoLvyx4zEkf3B5617b52WjXirLZ2Np_KRBPEw5TzDyUHiBlG-esLsTampW0nUBQ7M2JUuwFib0gsTcn_syJnY_eHfulU7IqrKyVcac8RglLGfLYwwHbmkp3_64Xg-HMDz4eHuLGtXp3iku7FCwhCRXfs5GY8PfJlH-8iifyCwfHj2k</recordid><startdate>199803</startdate><enddate>199803</enddate><creator>Moromisato, David Y.</creator><creator>Roberts Jr, Charles</creator><creator>Brasel, Jo Anne</creator><creator>Mohan, Subburaman</creator><creator>Cowles, Elizabeth</creator><creator>King, Stephen M.</creator><creator>Cooper, Dan M.</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>NAPCQ</scope></search><sort><creationdate>199803</creationdate><title>Erythrocyte insulin-like growth factor-I binding in younger and older males</title><author>Moromisato, David Y. ; Roberts Jr, Charles ; Brasel, Jo Anne ; Mohan, Subburaman ; Cowles, Elizabeth ; King, Stephen M. ; Cooper, Dan M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5205-1e110441a2bc86249bcfd5b332f102b962b73b3a384eb695937d9dc4f02bf9493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Biological and medical sciences</topic><topic>Cell receptors</topic><topic>Cell structures and functions</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors</topic><topic>Molecular and cellular biology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moromisato, David Y.</creatorcontrib><creatorcontrib>Roberts Jr, Charles</creatorcontrib><creatorcontrib>Brasel, Jo Anne</creatorcontrib><creatorcontrib>Mohan, Subburaman</creatorcontrib><creatorcontrib>Cowles, Elizabeth</creatorcontrib><creatorcontrib>King, Stephen M.</creatorcontrib><creatorcontrib>Cooper, Dan M.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><jtitle>Clinical endocrinology (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moromisato, David Y.</au><au>Roberts Jr, Charles</au><au>Brasel, Jo Anne</au><au>Mohan, Subburaman</au><au>Cowles, Elizabeth</au><au>King, Stephen M.</au><au>Cooper, Dan M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Erythrocyte insulin-like growth factor-I binding in younger and older males</atitle><jtitle>Clinical endocrinology (Oxford)</jtitle><addtitle>Clinical Endocrinology</addtitle><date>1998-03</date><risdate>1998</risdate><volume>48</volume><issue>3</issue><spage>339</spage><epage>345</epage><pages>339-345</pages><issn>0300-0664</issn><eissn>1365-2265</eissn><coden>CLECAP</coden><abstract>OBJECTIVE
Insulin‐like growth factor‐I (IGF‐I) levels are lower in older compared with younger subjects. We tested the hypothesis that the reduction in circulating IGF‐I would be accompanied by upregulation in tissue IGF‐I binding in at least some tissues. We tested erythrocyte IGF‐I binding since blood is an accessible tissue in humans, and there is growing evidence to suggest that erythrocyte IGF‐I binding is influenced by circulating IGF‐I.
DESIGN AND PATIENTS
We compared 9 healthy older males (61–68 years old) with 9 healthy younger males (15–19 years old).
MEASUREMENTS
Standard techniques were used to assay circulating IGF‐I and IGF binding proteins 1–5 (IGFBPs 1–5). Erythrocyte IGF‐I binding was first measured by studies in which native [125I]‐IGF‐I was displaced with unlabelled native IGF‐I. In order to determine a possible role for IGF binding proteins (IGFBP), native [125I]‐IGF‐I was displaced with des‐(1‐3)IGF‐I, which binds with IGF receptors but not IGFBPs.
RESULTS
As expected, circulating IGF‐I was significantly lower in the older compared with the younger subjects. In addition, IGFBP‐3 and 5 were significantly lower, and IGFBP‐4 higher, in older compared with younger subjects. When native [125I]‐IGF‐I was displaced with unlabelled native IGF‐I, the number of IGF‐I binding sites per erythrocyte was higher in the older subjects (43 ± 5 vs. 18 ± 2, older vs. younger, respectively; P < 0.05). In contrast, when native [125I]‐IGF‐I was displaced with des‐(1‐3), IGF‐I binding capacity was not different between the two age groups.
CONCLUSIONS
Erythrocyte IGF binding was increased in older compared with younger subjects. Surprisingly, the mechanism of the increase may not be a simple up regulation of IGF‐I receptors in response to reduced circulating IGF‐I, but possibly by an increase in the levels of as yet unidentified erythrocyte membrane‐associated IGF binding proteins.</abstract><cop>Oxford BSL</cop><pub>Blackwell Science Ltd</pub><doi>10.1046/j.1365-2265.1998.00395.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0300-0664 |
| ispartof | Clinical endocrinology (Oxford), 1998-03, Vol.48 (3), p.339-345 |
| issn | 0300-0664 1365-2265 |
| language | eng |
| recordid | cdi_proquest_journals_198790826 |
| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Biological and medical sciences Cell receptors Cell structures and functions Fundamental and applied biological sciences. Psychology Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors Molecular and cellular biology |
| title | Erythrocyte insulin-like growth factor-I binding in younger and older males |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T03%3A16%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Erythrocyte%20insulin-like%20growth%20factor-I%20binding%20in%20younger%20and%20older%20males&rft.jtitle=Clinical%20endocrinology%20(Oxford)&rft.au=Moromisato,%20David%20Y.&rft.date=1998-03&rft.volume=48&rft.issue=3&rft.spage=339&rft.epage=345&rft.pages=339-345&rft.issn=0300-0664&rft.eissn=1365-2265&rft.coden=CLECAP&rft_id=info:doi/10.1046/j.1365-2265.1998.00395.x&rft_dat=%3Cproquest_cross%3E28918059%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=198790826&rft_id=info:pmid/&rfr_iscdi=true |