Cost-effective and rapid (3 h) method for combined extraction of DNA, RNA and protein from a single tea leaf sample for genomic and proteomic analysis
This is the first report about the simultaneous extraction of nucleic acids and proteins from tea leaf tissue. Using the present protocol, the DNA, RNA and protein were simultaneously isolated from a single tea leaf sample. The method also maintained the quality and quantity of the isolated biomolec...
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| Veröffentlicht in: | Journal of plant biochemistry and biotechnology 2018, Vol.27 (1), p.100-107 |
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| container_end_page | 107 |
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| container_title | Journal of plant biochemistry and biotechnology |
| container_volume | 27 |
| creator | Hazarika, Pranita Ranjit Singh, H. |
| description | This is the first report about the simultaneous extraction of nucleic acids and proteins from tea leaf tissue. Using the present protocol, the DNA, RNA and protein were simultaneously isolated from a single tea leaf sample. The method also maintained the quality and quantity of the isolated biomolecules. The method is cost-effective and takes only 3 h to isolate the starting molecules (DNA, RNA and protein) of central dogma of biology. It was also demonstrated that the isolated DNA, RNA and protein could be successfully used for genomics and proteomic analysis in tea plant which was verified by performing marker study, gene cloning, cDNA preparation, gene expression study and 2-DE. |
| doi_str_mv | 10.1007/s13562-017-0420-z |
| format | Article |
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Using the present protocol, the DNA, RNA and protein were simultaneously isolated from a single tea leaf sample. The method also maintained the quality and quantity of the isolated biomolecules. The method is cost-effective and takes only 3 h to isolate the starting molecules (DNA, RNA and protein) of central dogma of biology. 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Plant Biochem. Biotechnol</addtitle><description>This is the first report about the simultaneous extraction of nucleic acids and proteins from tea leaf tissue. Using the present protocol, the DNA, RNA and protein were simultaneously isolated from a single tea leaf sample. The method also maintained the quality and quantity of the isolated biomolecules. The method is cost-effective and takes only 3 h to isolate the starting molecules (DNA, RNA and protein) of central dogma of biology. It was also demonstrated that the isolated DNA, RNA and protein could be successfully used for genomics and proteomic analysis in tea plant which was verified by performing marker study, gene cloning, cDNA preparation, gene expression study and 2-DE.</description><subject>Biomedical and Life Sciences</subject><subject>Biomolecules</subject><subject>Cell Biology</subject><subject>Cloning</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Gene expression</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Nucleic acids</subject><subject>Original Article</subject><subject>Plant Biochemistry</subject><subject>Plant tissues</subject><subject>Protein Science</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Receptors</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Tea</subject><issn>0971-7811</issn><issn>0974-1275</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kM1KAzEUhQdRsFYfwF3AjYLRJPOTZlnqL5QKoutwO3PTTulMajIV2xdx67P4ZKadgm7kLu69cL7D4UTRKWdXnDF57XmcZoIyLilLBKPrvajDlEwoFzLd396cyh7nh9GR9zPGklSypBN9DqxvKBqDeVO-I4G6IA4WZUHO4--v6QWpsJnaghjrSG6rcVljQfCjcRD0tibWkJtR_5I8j_pbduFsg2VNjLMVAeLLejJH0iCQOYIhHqpF-DduE6xtVea_1O6D-cqX_jg6MDD3eLLb3ej17vZl8ECHT_ePg_6Q5jHPGsoRBAcQMAZlJGbMmDH0ksKgVBlLwyGVEiaJ0bBYJBmqnGeQj1MTK5kKFXejs9Y3RHhbom_0zC5dCOE1Vz0ZpidEUPFWlTvrvUOjF66swK00Z3rTv27716F_velfrwMjWsYHbT1B98f5X-gH0EaKpA</recordid><startdate>2018</startdate><enddate>2018</enddate><creator>Hazarika, Pranita</creator><creator>Ranjit Singh, H.</creator><general>Springer India</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>2018</creationdate><title>Cost-effective and rapid (3 h) method for combined extraction of DNA, RNA and protein from a single tea leaf sample for genomic and proteomic analysis</title><author>Hazarika, Pranita ; Ranjit Singh, H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-1ea21aa2aba9f7e60ffba84dfe796054df7992f43ef03246e9c16acb5f3975293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biomolecules</topic><topic>Cell Biology</topic><topic>Cloning</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Gene expression</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Nucleic acids</topic><topic>Original Article</topic><topic>Plant Biochemistry</topic><topic>Plant tissues</topic><topic>Protein Science</topic><topic>Proteins</topic><topic>Proteomics</topic><topic>Receptors</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Tea</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hazarika, Pranita</creatorcontrib><creatorcontrib>Ranjit Singh, H.</creatorcontrib><collection>CrossRef</collection><jtitle>Journal of plant biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hazarika, Pranita</au><au>Ranjit Singh, H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cost-effective and rapid (3 h) method for combined extraction of DNA, RNA and protein from a single tea leaf sample for genomic and proteomic analysis</atitle><jtitle>Journal of plant biochemistry and biotechnology</jtitle><stitle>J. 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| ispartof | Journal of plant biochemistry and biotechnology, 2018, Vol.27 (1), p.100-107 |
| issn | 0971-7811 0974-1275 |
| language | eng |
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| source | SpringerNature Journals |
| subjects | Biomedical and Life Sciences Biomolecules Cell Biology Cloning Deoxyribonucleic acid DNA Gene expression Leaves Life Sciences Nucleic acids Original Article Plant Biochemistry Plant tissues Protein Science Proteins Proteomics Receptors Ribonucleic acid RNA Tea |
| title | Cost-effective and rapid (3 h) method for combined extraction of DNA, RNA and protein from a single tea leaf sample for genomic and proteomic analysis |
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