Comparison of in vitro and in vivo fertilizing potential of buffalo bull semen frozen in egg yolk‐, soya bean lecithin‐ and liposome‐based extenders
Contents The objective of this study was to compare different extenders for post‐thaw in vitro sperm function and in vivo fertility of buffalo semen. Accordingly, sperm of 30 ejaculates extended in egg yolk (TRIS with 20% egg yolk; EY), two soya lecithin‐based (SL‐1; AndroMed® and SL‐2; Bioxcell®) a...
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| Veröffentlicht in: | Reproduction in domestic animals 2018-02, Vol.53 (1), p.195-202 |
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| creator | Singh, AK Kumar, A Honparkhe, M Kaur, S Kaur, H Ghuman, SPS Brar, PS |
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The objective of this study was to compare different extenders for post‐thaw in vitro sperm function and in vivo fertility of buffalo semen. Accordingly, sperm of 30 ejaculates extended in egg yolk (TRIS with 20% egg yolk; EY), two soya lecithin‐based (SL‐1; AndroMed® and SL‐2; Bioxcell®) and a liposome‐based extender (LS; OptiXcell®) were tested. The post‐thaw semen was evaluated for computer‐assisted sperm analysis (CASA), sperm viability, membrane and acrosome integrity, DNA integrity and acrosome reaction and first service pregnancy rate (FSPR) in a fixed‐time artificial insemination programme. Total motility and VCL were the only CASA‐based parameters that exhibited significantly higher (p .05) in EY, SL‐1, SL‐2 and LS extender, respectively. However, a variable response in terms of efficacy of different extenders for sperm viability and plasma membrane integrity was observed. Assessment of inducibility of acrosome reaction showed significant differences between extenders (51.9 ± 2.1, 44.3 ± 2.4, 46.1 ± 2.3 and 58.1 ± 3.1%, respectively, for EY, SL‐1, SL‐2 and LS). Furthermore, field trials revealed significantly higher (p |
| doi_str_mv | 10.1111/rda.13092 |
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The objective of this study was to compare different extenders for post‐thaw in vitro sperm function and in vivo fertility of buffalo semen. Accordingly, sperm of 30 ejaculates extended in egg yolk (TRIS with 20% egg yolk; EY), two soya lecithin‐based (SL‐1; AndroMed® and SL‐2; Bioxcell®) and a liposome‐based extender (LS; OptiXcell®) were tested. The post‐thaw semen was evaluated for computer‐assisted sperm analysis (CASA), sperm viability, membrane and acrosome integrity, DNA integrity and acrosome reaction and first service pregnancy rate (FSPR) in a fixed‐time artificial insemination programme. Total motility and VCL were the only CASA‐based parameters that exhibited significantly higher (p < .05) percentage in LS among these extenders. Post‐thaw percentage of acrosome integrity (55.9 ± 1.4, 58.1 ± 2.0, 55.8 ± 2.0, 56.6 ± 2.3) and DNA integrity (68.8 ± 2.0, 69.2 ± 2.3, 71.3 ± 2.1, 69.1 ± 2.1) did not differ (p > .05) in EY, SL‐1, SL‐2 and LS extender, respectively. However, a variable response in terms of efficacy of different extenders for sperm viability and plasma membrane integrity was observed. Assessment of inducibility of acrosome reaction showed significant differences between extenders (51.9 ± 2.1, 44.3 ± 2.4, 46.1 ± 2.3 and 58.1 ± 3.1%, respectively, for EY, SL‐1, SL‐2 and LS). Furthermore, field trials revealed significantly higher (p < .05) FSPR of LS‐extended semen as compared to that for EY, SL‐1 and SL‐2 extender (46.3%, 41.2%, 31.2% and 29.7%, respectively). It is concluded that the liposome‐based extender is more effective than egg yolk‐ and soya lecithin‐based extenders and may be used for cryopreservation of buffalo semen in the future.</description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/rda.13092</identifier><identifier>PMID: 29080291</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Acrosome reaction ; Acrosome Reaction - drug effects ; Animals ; Artificial insemination ; Buffalo ; buffalo bull spermatozoa ; Buffaloes ; Cell Membrane - drug effects ; Cryopreservation ; Cryopreservation - veterinary ; Cryoprotective Agents ; Deoxyribonucleic acid ; DNA ; Egg Yolk ; Female ; Fertility ; Glycine max - chemistry ; Insemination, Artificial - veterinary ; Integrity ; Lecithin ; Lecithins ; liposome ; Liposomes ; Male ; Pregnancy ; Pregnancy Rate ; Reproduction (biology) ; Semen ; Semen Analysis - veterinary ; Semen Preservation - methods ; Semen Preservation - veterinary ; Sperm ; Sperm Motility - drug effects ; Spermatozoa - physiology ; Viability ; Yolk</subject><ispartof>Reproduction in domestic animals, 2018-02, Vol.53 (1), p.195-202</ispartof><rights>2017 Blackwell Verlag GmbH</rights><rights>2017 Blackwell Verlag GmbH.</rights><rights>Copyright © 2018 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3532-cd6ea0ba088916b27d46d33e21cc87cfd45c31b150db6096f1da18571c2abf983</citedby><cites>FETCH-LOGICAL-c3532-cd6ea0ba088916b27d46d33e21cc87cfd45c31b150db6096f1da18571c2abf983</cites><orcidid>0000-0003-2164-764X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Frda.13092$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Frda.13092$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29080291$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Singh, AK</creatorcontrib><creatorcontrib>Kumar, A</creatorcontrib><creatorcontrib>Honparkhe, M</creatorcontrib><creatorcontrib>Kaur, S</creatorcontrib><creatorcontrib>Kaur, H</creatorcontrib><creatorcontrib>Ghuman, SPS</creatorcontrib><creatorcontrib>Brar, PS</creatorcontrib><title>Comparison of in vitro and in vivo fertilizing potential of buffalo bull semen frozen in egg yolk‐, soya bean lecithin‐ and liposome‐based extenders</title><title>Reproduction in domestic animals</title><addtitle>Reprod Domest Anim</addtitle><description>Contents
The objective of this study was to compare different extenders for post‐thaw in vitro sperm function and in vivo fertility of buffalo semen. Accordingly, sperm of 30 ejaculates extended in egg yolk (TRIS with 20% egg yolk; EY), two soya lecithin‐based (SL‐1; AndroMed® and SL‐2; Bioxcell®) and a liposome‐based extender (LS; OptiXcell®) were tested. The post‐thaw semen was evaluated for computer‐assisted sperm analysis (CASA), sperm viability, membrane and acrosome integrity, DNA integrity and acrosome reaction and first service pregnancy rate (FSPR) in a fixed‐time artificial insemination programme. Total motility and VCL were the only CASA‐based parameters that exhibited significantly higher (p < .05) percentage in LS among these extenders. Post‐thaw percentage of acrosome integrity (55.9 ± 1.4, 58.1 ± 2.0, 55.8 ± 2.0, 56.6 ± 2.3) and DNA integrity (68.8 ± 2.0, 69.2 ± 2.3, 71.3 ± 2.1, 69.1 ± 2.1) did not differ (p > .05) in EY, SL‐1, SL‐2 and LS extender, respectively. However, a variable response in terms of efficacy of different extenders for sperm viability and plasma membrane integrity was observed. Assessment of inducibility of acrosome reaction showed significant differences between extenders (51.9 ± 2.1, 44.3 ± 2.4, 46.1 ± 2.3 and 58.1 ± 3.1%, respectively, for EY, SL‐1, SL‐2 and LS). Furthermore, field trials revealed significantly higher (p < .05) FSPR of LS‐extended semen as compared to that for EY, SL‐1 and SL‐2 extender (46.3%, 41.2%, 31.2% and 29.7%, respectively). It is concluded that the liposome‐based extender is more effective than egg yolk‐ and soya lecithin‐based extenders and may be used for cryopreservation of buffalo semen in the future.</description><subject>Acrosome reaction</subject><subject>Acrosome Reaction - drug effects</subject><subject>Animals</subject><subject>Artificial insemination</subject><subject>Buffalo</subject><subject>buffalo bull spermatozoa</subject><subject>Buffaloes</subject><subject>Cell Membrane - drug effects</subject><subject>Cryopreservation</subject><subject>Cryopreservation - veterinary</subject><subject>Cryoprotective Agents</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Egg Yolk</subject><subject>Female</subject><subject>Fertility</subject><subject>Glycine max - chemistry</subject><subject>Insemination, Artificial - veterinary</subject><subject>Integrity</subject><subject>Lecithin</subject><subject>Lecithins</subject><subject>liposome</subject><subject>Liposomes</subject><subject>Male</subject><subject>Pregnancy</subject><subject>Pregnancy Rate</subject><subject>Reproduction (biology)</subject><subject>Semen</subject><subject>Semen Analysis - veterinary</subject><subject>Semen Preservation - methods</subject><subject>Semen Preservation - veterinary</subject><subject>Sperm</subject><subject>Sperm Motility - drug effects</subject><subject>Spermatozoa - physiology</subject><subject>Viability</subject><subject>Yolk</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1u1DAUhS0EokNhwQsgS6yQSGvHE8deVsOvVAkJwTryz_Xg4tjBzrRMVzxC1308ngRPU9hxN0fX-u45kg9Czyk5oXVOs1UnlBHZPkArumayIR2jD9GKSMYb3nNxhJ6UckEI7UTfP0ZHrSSCtJKu0O0mjZPKvqSIk8M-4ks_54RVtMtymbCDPPvgr33c4inNEGevwoHWO-dUSFVDwAVGiNjldF2lnsJ2i_cpfP_96-Y1LmmvsAYVcQDj528-1ue7kOCnVNIIddeqgMXwsyZYyOUpelTdCzy712P09d3bL5sPzfmn9x83Z-eNYR1rG2M5KKIVEUJSrtverrllDFpqjOiNs-vOMKppR6zmRHJHraKi66lplXZSsGP0cvGdcvqxgzIPF2mXY40cqBQ9Y4zTA_VqoUxOpWRww5T9qPJ-oGQ4tDDUFoa7Fir74t5xp0ew_8i_316B0wW48gH2_3caPr85Wyz_AGkWljw</recordid><startdate>201802</startdate><enddate>201802</enddate><creator>Singh, AK</creator><creator>Kumar, A</creator><creator>Honparkhe, M</creator><creator>Kaur, S</creator><creator>Kaur, H</creator><creator>Ghuman, SPS</creator><creator>Brar, PS</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><orcidid>https://orcid.org/0000-0003-2164-764X</orcidid></search><sort><creationdate>201802</creationdate><title>Comparison of in vitro and in vivo fertilizing potential of buffalo bull semen frozen in egg yolk‐, soya bean lecithin‐ and liposome‐based extenders</title><author>Singh, AK ; Kumar, A ; Honparkhe, M ; Kaur, S ; Kaur, H ; Ghuman, SPS ; Brar, PS</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3532-cd6ea0ba088916b27d46d33e21cc87cfd45c31b150db6096f1da18571c2abf983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Acrosome reaction</topic><topic>Acrosome Reaction - drug effects</topic><topic>Animals</topic><topic>Artificial insemination</topic><topic>Buffalo</topic><topic>buffalo bull spermatozoa</topic><topic>Buffaloes</topic><topic>Cell Membrane - drug effects</topic><topic>Cryopreservation</topic><topic>Cryopreservation - veterinary</topic><topic>Cryoprotective Agents</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Egg Yolk</topic><topic>Female</topic><topic>Fertility</topic><topic>Glycine max - chemistry</topic><topic>Insemination, Artificial - veterinary</topic><topic>Integrity</topic><topic>Lecithin</topic><topic>Lecithins</topic><topic>liposome</topic><topic>Liposomes</topic><topic>Male</topic><topic>Pregnancy</topic><topic>Pregnancy Rate</topic><topic>Reproduction (biology)</topic><topic>Semen</topic><topic>Semen Analysis - veterinary</topic><topic>Semen Preservation - methods</topic><topic>Semen Preservation - veterinary</topic><topic>Sperm</topic><topic>Sperm Motility - drug effects</topic><topic>Spermatozoa - physiology</topic><topic>Viability</topic><topic>Yolk</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Singh, AK</creatorcontrib><creatorcontrib>Kumar, A</creatorcontrib><creatorcontrib>Honparkhe, M</creatorcontrib><creatorcontrib>Kaur, S</creatorcontrib><creatorcontrib>Kaur, H</creatorcontrib><creatorcontrib>Ghuman, SPS</creatorcontrib><creatorcontrib>Brar, PS</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Singh, AK</au><au>Kumar, A</au><au>Honparkhe, M</au><au>Kaur, S</au><au>Kaur, H</au><au>Ghuman, SPS</au><au>Brar, PS</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of in vitro and in vivo fertilizing potential of buffalo bull semen frozen in egg yolk‐, soya bean lecithin‐ and liposome‐based extenders</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Domest Anim</addtitle><date>2018-02</date><risdate>2018</risdate><volume>53</volume><issue>1</issue><spage>195</spage><epage>202</epage><pages>195-202</pages><issn>0936-6768</issn><eissn>1439-0531</eissn><abstract>Contents
The objective of this study was to compare different extenders for post‐thaw in vitro sperm function and in vivo fertility of buffalo semen. Accordingly, sperm of 30 ejaculates extended in egg yolk (TRIS with 20% egg yolk; EY), two soya lecithin‐based (SL‐1; AndroMed® and SL‐2; Bioxcell®) and a liposome‐based extender (LS; OptiXcell®) were tested. The post‐thaw semen was evaluated for computer‐assisted sperm analysis (CASA), sperm viability, membrane and acrosome integrity, DNA integrity and acrosome reaction and first service pregnancy rate (FSPR) in a fixed‐time artificial insemination programme. Total motility and VCL were the only CASA‐based parameters that exhibited significantly higher (p < .05) percentage in LS among these extenders. Post‐thaw percentage of acrosome integrity (55.9 ± 1.4, 58.1 ± 2.0, 55.8 ± 2.0, 56.6 ± 2.3) and DNA integrity (68.8 ± 2.0, 69.2 ± 2.3, 71.3 ± 2.1, 69.1 ± 2.1) did not differ (p > .05) in EY, SL‐1, SL‐2 and LS extender, respectively. However, a variable response in terms of efficacy of different extenders for sperm viability and plasma membrane integrity was observed. Assessment of inducibility of acrosome reaction showed significant differences between extenders (51.9 ± 2.1, 44.3 ± 2.4, 46.1 ± 2.3 and 58.1 ± 3.1%, respectively, for EY, SL‐1, SL‐2 and LS). Furthermore, field trials revealed significantly higher (p < .05) FSPR of LS‐extended semen as compared to that for EY, SL‐1 and SL‐2 extender (46.3%, 41.2%, 31.2% and 29.7%, respectively). It is concluded that the liposome‐based extender is more effective than egg yolk‐ and soya lecithin‐based extenders and may be used for cryopreservation of buffalo semen in the future.</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>29080291</pmid><doi>10.1111/rda.13092</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-2164-764X</orcidid></addata></record> |
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| subjects | Acrosome reaction Acrosome Reaction - drug effects Animals Artificial insemination Buffalo buffalo bull spermatozoa Buffaloes Cell Membrane - drug effects Cryopreservation Cryopreservation - veterinary Cryoprotective Agents Deoxyribonucleic acid DNA Egg Yolk Female Fertility Glycine max - chemistry Insemination, Artificial - veterinary Integrity Lecithin Lecithins liposome Liposomes Male Pregnancy Pregnancy Rate Reproduction (biology) Semen Semen Analysis - veterinary Semen Preservation - methods Semen Preservation - veterinary Sperm Sperm Motility - drug effects Spermatozoa - physiology Viability Yolk |
| title | Comparison of in vitro and in vivo fertilizing potential of buffalo bull semen frozen in egg yolk‐, soya bean lecithin‐ and liposome‐based extenders |
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