Resistance of phages lytic to pathogenic Escherichia coli to sanitisers used by the food industry and in home settings
Summary Phages are potentially useful as antimicrobial agents in food‐processing environments, provided they can remain active upon extended storage and in the presence of sanitisers. Survival of six phages lytic against enteropathogenic (EPEC) and shiga‐toxigenic (STEC) Escherichia coli strains was...
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description | Summary
Phages are potentially useful as antimicrobial agents in food‐processing environments, provided they can remain active upon extended storage and in the presence of sanitisers. Survival of six phages lytic against enteropathogenic (EPEC) and shiga‐toxigenic (STEC) Escherichia coli strains was assessed upon storage at 4 °C, −20 °C and −70 °C in phosphate‐buffered‐saline (PBS) and Tris‐magnesium‐gelatine buffer (TMG) for up to 1 year. The phages were also exposed to ethanol, sodium hypochlorite, peracetic acid, quaternary ammonium chloride (biocide A), hydrogen peroxide/peracetic acid/peroctanoic acid (biocide B), p‐toluensulfonchloroamide (biocide D) and alkaline chloride foam (biocide C). Plaque‐forming units remained unchanged when the phages were stored at 4 °C in both buffers tested, but decreased by 3.5 and 5.7 log10 PFU when stored in PBS at −20 and −70 °C, respectively. Moreover, TMG seems to be the most protective suspension medium with decreasing temperature because a 1−log10 PFU reduction was observed at −20 and −70 °C. Incubation in 100% ethanol for 24 h reduced plaque counts only by 2.5 log10 PFU. In 10 ppm of sodium hypochlorite and in biocide B (0.13%), the counts decreased by ~5 and ~6 log10 PFU after 15 min. Finally, biocide A and D reduced counts by 4 and 2–4 log10 PFU after 30 min and 8 h of incubation, respectively. Phages were completely inactivated only by peracetic acid and biocides C and E. Therefore, the phages evaluated might be potentially applied together with classical sanitisers such as ethanol and industrial biocides A, B and D, in disinfection programs against pathogenic STEC and EPEC strains.
Resistance of coliphages to sanitisers. |
doi_str_mv | 10.1111/ijfs.13626 |
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Phages are potentially useful as antimicrobial agents in food‐processing environments, provided they can remain active upon extended storage and in the presence of sanitisers. Survival of six phages lytic against enteropathogenic (EPEC) and shiga‐toxigenic (STEC) Escherichia coli strains was assessed upon storage at 4 °C, −20 °C and −70 °C in phosphate‐buffered‐saline (PBS) and Tris‐magnesium‐gelatine buffer (TMG) for up to 1 year. The phages were also exposed to ethanol, sodium hypochlorite, peracetic acid, quaternary ammonium chloride (biocide A), hydrogen peroxide/peracetic acid/peroctanoic acid (biocide B), p‐toluensulfonchloroamide (biocide D) and alkaline chloride foam (biocide C). Plaque‐forming units remained unchanged when the phages were stored at 4 °C in both buffers tested, but decreased by 3.5 and 5.7 log10 PFU when stored in PBS at −20 and −70 °C, respectively. Moreover, TMG seems to be the most protective suspension medium with decreasing temperature because a 1−log10 PFU reduction was observed at −20 and −70 °C. Incubation in 100% ethanol for 24 h reduced plaque counts only by 2.5 log10 PFU. In 10 ppm of sodium hypochlorite and in biocide B (0.13%), the counts decreased by ~5 and ~6 log10 PFU after 15 min. Finally, biocide A and D reduced counts by 4 and 2–4 log10 PFU after 30 min and 8 h of incubation, respectively. Phages were completely inactivated only by peracetic acid and biocides C and E. Therefore, the phages evaluated might be potentially applied together with classical sanitisers such as ethanol and industrial biocides A, B and D, in disinfection programs against pathogenic STEC and EPEC strains.
Resistance of coliphages to sanitisers.</description><identifier>ISSN: 0950-5423</identifier><identifier>EISSN: 1365-2621</identifier><identifier>DOI: 10.1111/ijfs.13626</identifier><language>eng</language><publisher>Oxford: Wiley Subscription Services, Inc</publisher><subject>Ammonium ; Ammonium chloride ; Antimicrobial agents ; Bacteriophage ; Biocides ; Buffers ; Chlorides ; Disinfection ; E coli ; Escherichia coli ; Ethanol ; Food industry ; Food processing ; Food processing industry ; Hydrogen peroxide ; Hydrogen storage ; Magnesium ; Oxidation ; Peracetic acid ; Peroctanoic acid ; phage viability ; Phages ; sanitiser ; Sodium ; Sodium hypochlorite</subject><ispartof>International journal of food science & technology, 2018-02, Vol.53 (2), p.533-540</ispartof><rights>2017 Institute of Food Science and Technology</rights><rights>International Journal of Food Science and Technology © 2018 Institute of Food Science and Technology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3746-dde4064641045b1e0066bf828ef5b35189fe5ae38720f50cfa70523d1ad8cb633</citedby><cites>FETCH-LOGICAL-c3746-dde4064641045b1e0066bf828ef5b35189fe5ae38720f50cfa70523d1ad8cb633</cites><orcidid>0000-0002-8640-0499</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fijfs.13626$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fijfs.13626$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids></links><search><creatorcontrib>Tomat, David</creatorcontrib><creatorcontrib>Balagué, Claudia</creatorcontrib><creatorcontrib>Aquili, Virginia</creatorcontrib><creatorcontrib>Verdini, Roxana</creatorcontrib><creatorcontrib>Quiberoni, Andrea</creatorcontrib><title>Resistance of phages lytic to pathogenic Escherichia coli to sanitisers used by the food industry and in home settings</title><title>International journal of food science & technology</title><description>Summary
Phages are potentially useful as antimicrobial agents in food‐processing environments, provided they can remain active upon extended storage and in the presence of sanitisers. Survival of six phages lytic against enteropathogenic (EPEC) and shiga‐toxigenic (STEC) Escherichia coli strains was assessed upon storage at 4 °C, −20 °C and −70 °C in phosphate‐buffered‐saline (PBS) and Tris‐magnesium‐gelatine buffer (TMG) for up to 1 year. The phages were also exposed to ethanol, sodium hypochlorite, peracetic acid, quaternary ammonium chloride (biocide A), hydrogen peroxide/peracetic acid/peroctanoic acid (biocide B), p‐toluensulfonchloroamide (biocide D) and alkaline chloride foam (biocide C). Plaque‐forming units remained unchanged when the phages were stored at 4 °C in both buffers tested, but decreased by 3.5 and 5.7 log10 PFU when stored in PBS at −20 and −70 °C, respectively. Moreover, TMG seems to be the most protective suspension medium with decreasing temperature because a 1−log10 PFU reduction was observed at −20 and −70 °C. Incubation in 100% ethanol for 24 h reduced plaque counts only by 2.5 log10 PFU. In 10 ppm of sodium hypochlorite and in biocide B (0.13%), the counts decreased by ~5 and ~6 log10 PFU after 15 min. Finally, biocide A and D reduced counts by 4 and 2–4 log10 PFU after 30 min and 8 h of incubation, respectively. Phages were completely inactivated only by peracetic acid and biocides C and E. Therefore, the phages evaluated might be potentially applied together with classical sanitisers such as ethanol and industrial biocides A, B and D, in disinfection programs against pathogenic STEC and EPEC strains.
Resistance of coliphages to sanitisers.</description><subject>Ammonium</subject><subject>Ammonium chloride</subject><subject>Antimicrobial agents</subject><subject>Bacteriophage</subject><subject>Biocides</subject><subject>Buffers</subject><subject>Chlorides</subject><subject>Disinfection</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Ethanol</subject><subject>Food industry</subject><subject>Food processing</subject><subject>Food processing industry</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen storage</subject><subject>Magnesium</subject><subject>Oxidation</subject><subject>Peracetic acid</subject><subject>Peroctanoic acid</subject><subject>phage viability</subject><subject>Phages</subject><subject>sanitiser</subject><subject>Sodium</subject><subject>Sodium hypochlorite</subject><issn>0950-5423</issn><issn>1365-2621</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kF1LwzAUhoMoOKc3_oKAd0Jn0jRZeylj6mQg-HFd0vRkzeiamZMp_fe2zmvPzcvLec4HLyHXnM34UHdua3HGhUrVCZkMKpNUpfyUTFghWSKzVJyTC8QtYywV82xCvl4BHUbdGaDe0n2jN4C07aMzNHq617HxG-gGt0TTQHCmcZoa37qxjbpz0SEEpAeEmlY9jQ1Q631NXVcfMIae6m40tPE7oAgxum6Dl-TM6hbh6k-n5ONh-b54StYvj6vF_Toxw3cqqWvImMpUxlkmKw6MKVXZPM3BykpInhcWpAaRz1NmJTNWz5lMRc11nZtKCTElN8e9--A_D4Cx3PpD6IaTJS9yWeS8EHygbo-UCR4xgC33we106EvOyjHXcsy1_M11gPkR_nYt9P-Q5er54e048wN5l3vg</recordid><startdate>201802</startdate><enddate>201802</enddate><creator>Tomat, David</creator><creator>Balagué, Claudia</creator><creator>Aquili, Virginia</creator><creator>Verdini, Roxana</creator><creator>Quiberoni, Andrea</creator><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7ST</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>SOI</scope><orcidid>https://orcid.org/0000-0002-8640-0499</orcidid></search><sort><creationdate>201802</creationdate><title>Resistance of phages lytic to pathogenic Escherichia coli to sanitisers used by the food industry and in home settings</title><author>Tomat, David ; Balagué, Claudia ; Aquili, Virginia ; Verdini, Roxana ; Quiberoni, Andrea</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3746-dde4064641045b1e0066bf828ef5b35189fe5ae38720f50cfa70523d1ad8cb633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Ammonium</topic><topic>Ammonium chloride</topic><topic>Antimicrobial agents</topic><topic>Bacteriophage</topic><topic>Biocides</topic><topic>Buffers</topic><topic>Chlorides</topic><topic>Disinfection</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Ethanol</topic><topic>Food industry</topic><topic>Food processing</topic><topic>Food processing industry</topic><topic>Hydrogen peroxide</topic><topic>Hydrogen storage</topic><topic>Magnesium</topic><topic>Oxidation</topic><topic>Peracetic acid</topic><topic>Peroctanoic acid</topic><topic>phage viability</topic><topic>Phages</topic><topic>sanitiser</topic><topic>Sodium</topic><topic>Sodium hypochlorite</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tomat, David</creatorcontrib><creatorcontrib>Balagué, Claudia</creatorcontrib><creatorcontrib>Aquili, Virginia</creatorcontrib><creatorcontrib>Verdini, Roxana</creatorcontrib><creatorcontrib>Quiberoni, Andrea</creatorcontrib><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><jtitle>International journal of food science & technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tomat, David</au><au>Balagué, Claudia</au><au>Aquili, Virginia</au><au>Verdini, Roxana</au><au>Quiberoni, Andrea</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Resistance of phages lytic to pathogenic Escherichia coli to sanitisers used by the food industry and in home settings</atitle><jtitle>International journal of food science & technology</jtitle><date>2018-02</date><risdate>2018</risdate><volume>53</volume><issue>2</issue><spage>533</spage><epage>540</epage><pages>533-540</pages><issn>0950-5423</issn><eissn>1365-2621</eissn><abstract>Summary
Phages are potentially useful as antimicrobial agents in food‐processing environments, provided they can remain active upon extended storage and in the presence of sanitisers. Survival of six phages lytic against enteropathogenic (EPEC) and shiga‐toxigenic (STEC) Escherichia coli strains was assessed upon storage at 4 °C, −20 °C and −70 °C in phosphate‐buffered‐saline (PBS) and Tris‐magnesium‐gelatine buffer (TMG) for up to 1 year. The phages were also exposed to ethanol, sodium hypochlorite, peracetic acid, quaternary ammonium chloride (biocide A), hydrogen peroxide/peracetic acid/peroctanoic acid (biocide B), p‐toluensulfonchloroamide (biocide D) and alkaline chloride foam (biocide C). Plaque‐forming units remained unchanged when the phages were stored at 4 °C in both buffers tested, but decreased by 3.5 and 5.7 log10 PFU when stored in PBS at −20 and −70 °C, respectively. Moreover, TMG seems to be the most protective suspension medium with decreasing temperature because a 1−log10 PFU reduction was observed at −20 and −70 °C. Incubation in 100% ethanol for 24 h reduced plaque counts only by 2.5 log10 PFU. In 10 ppm of sodium hypochlorite and in biocide B (0.13%), the counts decreased by ~5 and ~6 log10 PFU after 15 min. Finally, biocide A and D reduced counts by 4 and 2–4 log10 PFU after 30 min and 8 h of incubation, respectively. Phages were completely inactivated only by peracetic acid and biocides C and E. Therefore, the phages evaluated might be potentially applied together with classical sanitisers such as ethanol and industrial biocides A, B and D, in disinfection programs against pathogenic STEC and EPEC strains.
Resistance of coliphages to sanitisers.</abstract><cop>Oxford</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1111/ijfs.13626</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-8640-0499</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Ammonium Ammonium chloride Antimicrobial agents Bacteriophage Biocides Buffers Chlorides Disinfection E coli Escherichia coli Ethanol Food industry Food processing Food processing industry Hydrogen peroxide Hydrogen storage Magnesium Oxidation Peracetic acid Peroctanoic acid phage viability Phages sanitiser Sodium Sodium hypochlorite |
title | Resistance of phages lytic to pathogenic Escherichia coli to sanitisers used by the food industry and in home settings |
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