Purification and biochemical characterization of a cyclodextrin glycosyltransferase from Geobacillus thermoglucosidans CHB1

A strain named Geobacillus thermoglucosidans CHB1 was isolated from stockpiled hen dung compost. The cyclodextrin glycosyltransferase (CGTase) from the fermentation broth of this microorganism was purified 350.50‐fold at a yield of 9.33% through ammonium sulfate precipitation, DEAE‐Sepharose Fast Fl...

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Veröffentlicht in:	Starch 2018-01, Vol.70 (1-2), p.n/a
Hauptverfasser:	Jia, Xianbo, Ye, Xuejun, Chen, Jichen, Lin, Xinjian, Vasseur, Liette, You, Minsheng
Format:	Artikel
Sprache:	eng
Schlagworte:	Aluminum chloride Ammonium Ammonium sulfate Barium chloride Chromatography Composts Cyclodextrin Cyclodextrins Dung Electrophoresis Enzymes Fermentation Gel electrophoresis Geobacillus Glycosyltransferase Industrial production Iron chlorides Lithium chloride Maltodextrin Optimization pH effects Potatoes Poultry manure Purification Sodium Sodium dodecyl sulfate Sodium lauryl sulfate Soluble starch Starch Substrates Sulfates Temperature Thermostability
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