16S rRNA Gene Amplicon Sequencing Reveals Significant Changes in Microbial Compositions during Cyanobacteria-Laden Drinking Water Sludge Storage
This is the first study to systematically investigate the microbial community structure in cyanobacteria-laden drinking water sludge generated by different types of coagulants (including AlCl3, FeCl3, and polymeric aluminum ferric chloride (PAFC)) using Illumina 16S rRNA gene MiSeq sequencing. Resul...
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| Veröffentlicht in: | Environmental science & technology 2017-11, Vol.51 (21), p.12774-12783 |
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| creator | Pei, Haiyan Xu, Hangzhou Wang, Jingjing Jin, Yan Xiao, Hongdi Ma, Chunxia Sun, Jiongming Li, Hongmin |
| description | This is the first study to systematically investigate the microbial community structure in cyanobacteria-laden drinking water sludge generated by different types of coagulants (including AlCl3, FeCl3, and polymeric aluminum ferric chloride (PAFC)) using Illumina 16S rRNA gene MiSeq sequencing. Results show that Cyanobacteria, Proteobacteria, Firmicutes, Bacteroidetes, Verrucomicrobia, and Planctomycetes were the most dominant phyla in sludge, and because of the toxicity of high Al and Fe level in AlCl3 and FeCl3 sludges, respectively, the PAFC sludge exhibited greater microbial richness than that in AlCl3 and FeCl3 sludges. Due to lack of light and oxygen in sludge, relative abundance of the dominant genera Microcystis, Rhodobacter, Phenylobacterium, and Hydrogenophaga clearly decreased, especially after 4 days storage, and the amounts of extracellular microcystin and organic matter rose. As a result, the relative abundance of microcystin and organic degradation bacteria increased significantly, including pathogens such as Bacillus cereus, in particular after 4 days storage. Hence, sludge should be disposed of within 4 days to prevent massive growth of pathogens. In addition, because the increase of extracellular microcystins, organic matter, and pathogens in AlCl3 sludge was higher than that in FeCl3 and PAFC sludges, FeCl3 and PAFC may be ideal coagulants in drinking water treatment plants. |
| doi_str_mv | 10.1021/acs.est.7b03085 |
| format | Article |
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Results show that Cyanobacteria, Proteobacteria, Firmicutes, Bacteroidetes, Verrucomicrobia, and Planctomycetes were the most dominant phyla in sludge, and because of the toxicity of high Al and Fe level in AlCl3 and FeCl3 sludges, respectively, the PAFC sludge exhibited greater microbial richness than that in AlCl3 and FeCl3 sludges. Due to lack of light and oxygen in sludge, relative abundance of the dominant genera Microcystis, Rhodobacter, Phenylobacterium, and Hydrogenophaga clearly decreased, especially after 4 days storage, and the amounts of extracellular microcystin and organic matter rose. As a result, the relative abundance of microcystin and organic degradation bacteria increased significantly, including pathogens such as Bacillus cereus, in particular after 4 days storage. Hence, sludge should be disposed of within 4 days to prevent massive growth of pathogens. In addition, because the increase of extracellular microcystins, organic matter, and pathogens in AlCl3 sludge was higher than that in FeCl3 and PAFC sludges, FeCl3 and PAFC may be ideal coagulants in drinking water treatment plants.</description><identifier>ISSN: 0013-936X</identifier><identifier>EISSN: 1520-5851</identifier><identifier>DOI: 10.1021/acs.est.7b03085</identifier><identifier>PMID: 28994596</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Abundance ; Aluminum ; Aluminum chloride ; Bacteria ; Biodegradation ; Coagulants ; Community structure ; Contamination ; Cyanobacteria ; Drinking Water ; Ferric chloride ; Gene sequencing ; Iron chlorides ; Microcystins ; Microcystis ; Microorganisms ; Organic matter ; Pathogens ; Relative abundance ; RNA, Ribosomal, 16S ; rRNA 16S ; Sewage ; Sludge ; Storage ; Toxicity ; Water Purification ; Water treatment ; Water treatment plants</subject><ispartof>Environmental science & technology, 2017-11, Vol.51 (21), p.12774-12783</ispartof><rights>Copyright © 2017 American Chemical Society</rights><rights>Copyright American Chemical Society Nov 7, 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a361t-5d29d7ab42bb8f6cf861acf3baa9ea1451a27d601555b35dc7018195dd885bd83</citedby><cites>FETCH-LOGICAL-a361t-5d29d7ab42bb8f6cf861acf3baa9ea1451a27d601555b35dc7018195dd885bd83</cites><orcidid>0000-0001-6602-4740</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.est.7b03085$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.est.7b03085$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2763,27074,27922,27923,56736,56786</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28994596$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pei, Haiyan</creatorcontrib><creatorcontrib>Xu, Hangzhou</creatorcontrib><creatorcontrib>Wang, Jingjing</creatorcontrib><creatorcontrib>Jin, Yan</creatorcontrib><creatorcontrib>Xiao, Hongdi</creatorcontrib><creatorcontrib>Ma, Chunxia</creatorcontrib><creatorcontrib>Sun, Jiongming</creatorcontrib><creatorcontrib>Li, Hongmin</creatorcontrib><title>16S rRNA Gene Amplicon Sequencing Reveals Significant Changes in Microbial Compositions during Cyanobacteria-Laden Drinking Water Sludge Storage</title><title>Environmental science & technology</title><addtitle>Environ. Sci. Technol</addtitle><description>This is the first study to systematically investigate the microbial community structure in cyanobacteria-laden drinking water sludge generated by different types of coagulants (including AlCl3, FeCl3, and polymeric aluminum ferric chloride (PAFC)) using Illumina 16S rRNA gene MiSeq sequencing. Results show that Cyanobacteria, Proteobacteria, Firmicutes, Bacteroidetes, Verrucomicrobia, and Planctomycetes were the most dominant phyla in sludge, and because of the toxicity of high Al and Fe level in AlCl3 and FeCl3 sludges, respectively, the PAFC sludge exhibited greater microbial richness than that in AlCl3 and FeCl3 sludges. Due to lack of light and oxygen in sludge, relative abundance of the dominant genera Microcystis, Rhodobacter, Phenylobacterium, and Hydrogenophaga clearly decreased, especially after 4 days storage, and the amounts of extracellular microcystin and organic matter rose. As a result, the relative abundance of microcystin and organic degradation bacteria increased significantly, including pathogens such as Bacillus cereus, in particular after 4 days storage. Hence, sludge should be disposed of within 4 days to prevent massive growth of pathogens. In addition, because the increase of extracellular microcystins, organic matter, and pathogens in AlCl3 sludge was higher than that in FeCl3 and PAFC sludges, FeCl3 and PAFC may be ideal coagulants in drinking water treatment plants.</description><subject>Abundance</subject><subject>Aluminum</subject><subject>Aluminum chloride</subject><subject>Bacteria</subject><subject>Biodegradation</subject><subject>Coagulants</subject><subject>Community structure</subject><subject>Contamination</subject><subject>Cyanobacteria</subject><subject>Drinking Water</subject><subject>Ferric chloride</subject><subject>Gene sequencing</subject><subject>Iron chlorides</subject><subject>Microcystins</subject><subject>Microcystis</subject><subject>Microorganisms</subject><subject>Organic matter</subject><subject>Pathogens</subject><subject>Relative abundance</subject><subject>RNA, Ribosomal, 16S</subject><subject>rRNA 16S</subject><subject>Sewage</subject><subject>Sludge</subject><subject>Storage</subject><subject>Toxicity</subject><subject>Water Purification</subject><subject>Water treatment</subject><subject>Water treatment plants</subject><issn>0013-936X</issn><issn>1520-5851</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUtP3DAUha0KVKaUNTtkiSXKYCfjjL0chUcrDUUircouun4kGGbswU4q8S_6k3E0A6xYWfL9zjm69yB0TMmUkpyeg4pTE_vpXJKCcPYFTSjLScY4o3toQggtMlGU9wfoW4yPhJA8UV_RQc6FmDFRTtB_WtY43P1a4GvjDF6sNyurvMO1eR6MU9Z1-M78M7CKuLads61V4HpcPYDrTMTW4RurgpcWVrjy642PtrfeRayHMIqrF3BegupNsJAtQRuHL9LkaRz-hfSN69WgO4Pr3gfozHe036Y0c7R7D9Gfq8vf1Y9seXv9s1osMyhK2mdM50LPQc5yKXlbqpaXFFRbSABhgM4YhXyuS0IZY7JgWs0J5VQwrTlnUvPiEJ1ufTfBp1Vj3zz6IbgU2VCRzlfwXLBEnW-ptGOMwbTNJtg1hJeGkmZsoEkNNKN610BSnOx8B7k2-p1_O3kCzrbAqPzI_MTuFbf_kyc</recordid><startdate>20171107</startdate><enddate>20171107</enddate><creator>Pei, Haiyan</creator><creator>Xu, Hangzhou</creator><creator>Wang, Jingjing</creator><creator>Jin, Yan</creator><creator>Xiao, Hongdi</creator><creator>Ma, Chunxia</creator><creator>Sun, Jiongming</creator><creator>Li, Hongmin</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><orcidid>https://orcid.org/0000-0001-6602-4740</orcidid></search><sort><creationdate>20171107</creationdate><title>16S rRNA Gene Amplicon Sequencing Reveals Significant Changes in Microbial Compositions during Cyanobacteria-Laden Drinking Water Sludge Storage</title><author>Pei, Haiyan ; Xu, Hangzhou ; Wang, Jingjing ; Jin, Yan ; Xiao, Hongdi ; Ma, Chunxia ; Sun, Jiongming ; Li, Hongmin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a361t-5d29d7ab42bb8f6cf861acf3baa9ea1451a27d601555b35dc7018195dd885bd83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Abundance</topic><topic>Aluminum</topic><topic>Aluminum chloride</topic><topic>Bacteria</topic><topic>Biodegradation</topic><topic>Coagulants</topic><topic>Community structure</topic><topic>Contamination</topic><topic>Cyanobacteria</topic><topic>Drinking Water</topic><topic>Ferric chloride</topic><topic>Gene sequencing</topic><topic>Iron chlorides</topic><topic>Microcystins</topic><topic>Microcystis</topic><topic>Microorganisms</topic><topic>Organic matter</topic><topic>Pathogens</topic><topic>Relative abundance</topic><topic>RNA, Ribosomal, 16S</topic><topic>rRNA 16S</topic><topic>Sewage</topic><topic>Sludge</topic><topic>Storage</topic><topic>Toxicity</topic><topic>Water Purification</topic><topic>Water treatment</topic><topic>Water treatment plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pei, Haiyan</creatorcontrib><creatorcontrib>Xu, Hangzhou</creatorcontrib><creatorcontrib>Wang, Jingjing</creatorcontrib><creatorcontrib>Jin, Yan</creatorcontrib><creatorcontrib>Xiao, Hongdi</creatorcontrib><creatorcontrib>Ma, Chunxia</creatorcontrib><creatorcontrib>Sun, Jiongming</creatorcontrib><creatorcontrib>Li, Hongmin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Environmental science & technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pei, Haiyan</au><au>Xu, Hangzhou</au><au>Wang, Jingjing</au><au>Jin, Yan</au><au>Xiao, Hongdi</au><au>Ma, Chunxia</au><au>Sun, Jiongming</au><au>Li, Hongmin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>16S rRNA Gene Amplicon Sequencing Reveals Significant Changes in Microbial Compositions during Cyanobacteria-Laden Drinking Water Sludge Storage</atitle><jtitle>Environmental science & technology</jtitle><addtitle>Environ. Sci. Technol</addtitle><date>2017-11-07</date><risdate>2017</risdate><volume>51</volume><issue>21</issue><spage>12774</spage><epage>12783</epage><pages>12774-12783</pages><issn>0013-936X</issn><eissn>1520-5851</eissn><abstract>This is the first study to systematically investigate the microbial community structure in cyanobacteria-laden drinking water sludge generated by different types of coagulants (including AlCl3, FeCl3, and polymeric aluminum ferric chloride (PAFC)) using Illumina 16S rRNA gene MiSeq sequencing. Results show that Cyanobacteria, Proteobacteria, Firmicutes, Bacteroidetes, Verrucomicrobia, and Planctomycetes were the most dominant phyla in sludge, and because of the toxicity of high Al and Fe level in AlCl3 and FeCl3 sludges, respectively, the PAFC sludge exhibited greater microbial richness than that in AlCl3 and FeCl3 sludges. Due to lack of light and oxygen in sludge, relative abundance of the dominant genera Microcystis, Rhodobacter, Phenylobacterium, and Hydrogenophaga clearly decreased, especially after 4 days storage, and the amounts of extracellular microcystin and organic matter rose. As a result, the relative abundance of microcystin and organic degradation bacteria increased significantly, including pathogens such as Bacillus cereus, in particular after 4 days storage. Hence, sludge should be disposed of within 4 days to prevent massive growth of pathogens. In addition, because the increase of extracellular microcystins, organic matter, and pathogens in AlCl3 sludge was higher than that in FeCl3 and PAFC sludges, FeCl3 and PAFC may be ideal coagulants in drinking water treatment plants.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>28994596</pmid><doi>10.1021/acs.est.7b03085</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-6602-4740</orcidid></addata></record> |
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| ispartof | Environmental science & technology, 2017-11, Vol.51 (21), p.12774-12783 |
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| subjects | Abundance Aluminum Aluminum chloride Bacteria Biodegradation Coagulants Community structure Contamination Cyanobacteria Drinking Water Ferric chloride Gene sequencing Iron chlorides Microcystins Microcystis Microorganisms Organic matter Pathogens Relative abundance RNA, Ribosomal, 16S rRNA 16S Sewage Sludge Storage Toxicity Water Purification Water treatment Water treatment plants |
| title | 16S rRNA Gene Amplicon Sequencing Reveals Significant Changes in Microbial Compositions during Cyanobacteria-Laden Drinking Water Sludge Storage |
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