The cellular pathway and enzymatic activity for phloem-unloading transition in developing Camellia oleifera Abel. Fruit

The phloem-unloading pathway of sucrose and mechanism of sugar-to-oil transition are still unknown in Camellia oleifera Fruit. Here, transmission electronic microscopy (TEM) and confocal laser-scanning microscopy (CLSM) were used to observe the cellular structure of vascular bundles and symplastic t...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Acta physiologiae plantarum 2018-02, Vol.40 (2), p.1-11, Article 23
Hauptverfasser: Wang, Xiaoyi, You, Hanli, Yuan, Yihang, Zhang, Hehua, Zhang, Lingyun
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 11
container_issue 2
container_start_page 1
container_title Acta physiologiae plantarum
container_volume 40
creator Wang, Xiaoyi
You, Hanli
Yuan, Yihang
Zhang, Hehua
Zhang, Lingyun
description The phloem-unloading pathway of sucrose and mechanism of sugar-to-oil transition are still unknown in Camellia oleifera Fruit. Here, transmission electronic microscopy (TEM) and confocal laser-scanning microscopy (CLSM) were used to observe the cellular structure of vascular bundles and symplastic tracer, carboxyfluorescein (CF), transport in phloem zone. The results showed that sucrose was transported via symplast system in the early and late phases, whereas apoplast system exerted the function in middle stage. Moreover, enzymatic assays showed that acid invertase had a higher activity at the transition stage during the whole fruit development. The cell wall bound invertase (CWI) activity reached the highest at the middle stage of fruit development and the switch in phloem-unloading coincided with fruit developmental phase change and oil accumulation. Correlation analysis showed that the oil accumulation was significantly negatively correlated with content of soluble sugar at P  
doi_str_mv 10.1007/s11738-017-2598-z
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1984769960</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1984769960</sourcerecordid><originalsourceid>FETCH-LOGICAL-c316t-2e4c5fa24d7b890c4e09ff951d12acc19b85961b9eadd29084a26c0856fe72723</originalsourceid><addsrcrecordid>eNp1kD1PIzEURa0VK21g-QF0lqjN-nlmPHaJIj5WQqIJteV43hAjxw62B5T8eiYKxTZbveKee590CLkCfgOc938KQN8oxqFnotOKHX6QBSgJDKRsz8iCQ9OzTin4Rc5LeeO8azopF-RztUHqMIQp2Ex3tm4-7Z7aOFCMh_3WVu-oddV_-LqnY5qRTUi4ZVMMyQ4-vtKabSy--hSpj3TADwxpdwyWdjvvektTQD9itvR2jeGG3ufJ19_k52hDwcvve0Fe7u9Wy0f29Pzwd3n7xFwDsjKBretGK9qhXyvNXYtcj6PuYABhnQO9Vp2WsNZoh0ForlorpOOqkyP2ohfNBbk-7e5yep-wVPOWphznlwa0anupteQzBSfK5VRKxtHsst_avDfAzdGvOfk1s19z9GsOc0ecOmVm4yvmf5b_W_oCw-2AVA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1984769960</pqid></control><display><type>article</type><title>The cellular pathway and enzymatic activity for phloem-unloading transition in developing Camellia oleifera Abel. Fruit</title><source>SpringerNature Journals</source><creator>Wang, Xiaoyi ; You, Hanli ; Yuan, Yihang ; Zhang, Hehua ; Zhang, Lingyun</creator><creatorcontrib>Wang, Xiaoyi ; You, Hanli ; Yuan, Yihang ; Zhang, Hehua ; Zhang, Lingyun</creatorcontrib><description>The phloem-unloading pathway of sucrose and mechanism of sugar-to-oil transition are still unknown in Camellia oleifera Fruit. Here, transmission electronic microscopy (TEM) and confocal laser-scanning microscopy (CLSM) were used to observe the cellular structure of vascular bundles and symplastic tracer, carboxyfluorescein (CF), transport in phloem zone. The results showed that sucrose was transported via symplast system in the early and late phases, whereas apoplast system exerted the function in middle stage. Moreover, enzymatic assays showed that acid invertase had a higher activity at the transition stage during the whole fruit development. The cell wall bound invertase (CWI) activity reached the highest at the middle stage of fruit development and the switch in phloem-unloading coincided with fruit developmental phase change and oil accumulation. Correlation analysis showed that the oil accumulation was significantly negatively correlated with content of soluble sugar at P  &lt; 0.05 level. However, the soluble acid invertase (SAI), CWI, and neutral invertase showed a significant positive correlation with oil accumulation at P  &lt; 0.01 level. In summary, our data provide new cytological insights into the transition of unloading transfer between symplasmic and apoplasmic patterns in C. oleifera fruit and suggest that invertases are positively involved in sugar–oil transition process.</description><identifier>ISSN: 0137-5881</identifier><identifier>EISSN: 1861-1664</identifier><identifier>DOI: 10.1007/s11738-017-2598-z</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Accumulation ; Acids ; Agriculture ; Apoplast ; Biomedical and Life Sciences ; Camellia ; Cell walls ; Cellular structure ; Correlation analysis ; Developmental stages ; Enzymatic activity ; Fruits ; Invertase ; Life Sciences ; Microscopy ; Oil ; Original Article ; Phase transitions ; Phloem ; Plant Anatomy/Development ; Plant Biochemistry ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Scanning microscopy ; Sucrose ; Sugar ; Unloading</subject><ispartof>Acta physiologiae plantarum, 2018-02, Vol.40 (2), p.1-11, Article 23</ispartof><rights>Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2018</rights><rights>Copyright Springer Science &amp; Business Media 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-2e4c5fa24d7b890c4e09ff951d12acc19b85961b9eadd29084a26c0856fe72723</citedby><cites>FETCH-LOGICAL-c316t-2e4c5fa24d7b890c4e09ff951d12acc19b85961b9eadd29084a26c0856fe72723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11738-017-2598-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11738-017-2598-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,782,786,27931,27932,41495,42564,51326</link.rule.ids></links><search><creatorcontrib>Wang, Xiaoyi</creatorcontrib><creatorcontrib>You, Hanli</creatorcontrib><creatorcontrib>Yuan, Yihang</creatorcontrib><creatorcontrib>Zhang, Hehua</creatorcontrib><creatorcontrib>Zhang, Lingyun</creatorcontrib><title>The cellular pathway and enzymatic activity for phloem-unloading transition in developing Camellia oleifera Abel. Fruit</title><title>Acta physiologiae plantarum</title><addtitle>Acta Physiol Plant</addtitle><description>The phloem-unloading pathway of sucrose and mechanism of sugar-to-oil transition are still unknown in Camellia oleifera Fruit. Here, transmission electronic microscopy (TEM) and confocal laser-scanning microscopy (CLSM) were used to observe the cellular structure of vascular bundles and symplastic tracer, carboxyfluorescein (CF), transport in phloem zone. The results showed that sucrose was transported via symplast system in the early and late phases, whereas apoplast system exerted the function in middle stage. Moreover, enzymatic assays showed that acid invertase had a higher activity at the transition stage during the whole fruit development. The cell wall bound invertase (CWI) activity reached the highest at the middle stage of fruit development and the switch in phloem-unloading coincided with fruit developmental phase change and oil accumulation. Correlation analysis showed that the oil accumulation was significantly negatively correlated with content of soluble sugar at P  &lt; 0.05 level. However, the soluble acid invertase (SAI), CWI, and neutral invertase showed a significant positive correlation with oil accumulation at P  &lt; 0.01 level. In summary, our data provide new cytological insights into the transition of unloading transfer between symplasmic and apoplasmic patterns in C. oleifera fruit and suggest that invertases are positively involved in sugar–oil transition process.</description><subject>Accumulation</subject><subject>Acids</subject><subject>Agriculture</subject><subject>Apoplast</subject><subject>Biomedical and Life Sciences</subject><subject>Camellia</subject><subject>Cell walls</subject><subject>Cellular structure</subject><subject>Correlation analysis</subject><subject>Developmental stages</subject><subject>Enzymatic activity</subject><subject>Fruits</subject><subject>Invertase</subject><subject>Life Sciences</subject><subject>Microscopy</subject><subject>Oil</subject><subject>Original Article</subject><subject>Phase transitions</subject><subject>Phloem</subject><subject>Plant Anatomy/Development</subject><subject>Plant Biochemistry</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Scanning microscopy</subject><subject>Sucrose</subject><subject>Sugar</subject><subject>Unloading</subject><issn>0137-5881</issn><issn>1861-1664</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kD1PIzEURa0VK21g-QF0lqjN-nlmPHaJIj5WQqIJteV43hAjxw62B5T8eiYKxTZbveKee590CLkCfgOc938KQN8oxqFnotOKHX6QBSgJDKRsz8iCQ9OzTin4Rc5LeeO8azopF-RztUHqMIQp2Ex3tm4-7Z7aOFCMh_3WVu-oddV_-LqnY5qRTUi4ZVMMyQ4-vtKabSy--hSpj3TADwxpdwyWdjvvektTQD9itvR2jeGG3ufJ19_k52hDwcvve0Fe7u9Wy0f29Pzwd3n7xFwDsjKBretGK9qhXyvNXYtcj6PuYABhnQO9Vp2WsNZoh0ForlorpOOqkyP2ohfNBbk-7e5yep-wVPOWphznlwa0anupteQzBSfK5VRKxtHsst_avDfAzdGvOfk1s19z9GsOc0ecOmVm4yvmf5b_W_oCw-2AVA</recordid><startdate>20180201</startdate><enddate>20180201</enddate><creator>Wang, Xiaoyi</creator><creator>You, Hanli</creator><creator>Yuan, Yihang</creator><creator>Zhang, Hehua</creator><creator>Zhang, Lingyun</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20180201</creationdate><title>The cellular pathway and enzymatic activity for phloem-unloading transition in developing Camellia oleifera Abel. Fruit</title><author>Wang, Xiaoyi ; You, Hanli ; Yuan, Yihang ; Zhang, Hehua ; Zhang, Lingyun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-2e4c5fa24d7b890c4e09ff951d12acc19b85961b9eadd29084a26c0856fe72723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Accumulation</topic><topic>Acids</topic><topic>Agriculture</topic><topic>Apoplast</topic><topic>Biomedical and Life Sciences</topic><topic>Camellia</topic><topic>Cell walls</topic><topic>Cellular structure</topic><topic>Correlation analysis</topic><topic>Developmental stages</topic><topic>Enzymatic activity</topic><topic>Fruits</topic><topic>Invertase</topic><topic>Life Sciences</topic><topic>Microscopy</topic><topic>Oil</topic><topic>Original Article</topic><topic>Phase transitions</topic><topic>Phloem</topic><topic>Plant Anatomy/Development</topic><topic>Plant Biochemistry</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Scanning microscopy</topic><topic>Sucrose</topic><topic>Sugar</topic><topic>Unloading</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Xiaoyi</creatorcontrib><creatorcontrib>You, Hanli</creatorcontrib><creatorcontrib>Yuan, Yihang</creatorcontrib><creatorcontrib>Zhang, Hehua</creatorcontrib><creatorcontrib>Zhang, Lingyun</creatorcontrib><collection>CrossRef</collection><jtitle>Acta physiologiae plantarum</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Xiaoyi</au><au>You, Hanli</au><au>Yuan, Yihang</au><au>Zhang, Hehua</au><au>Zhang, Lingyun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The cellular pathway and enzymatic activity for phloem-unloading transition in developing Camellia oleifera Abel. Fruit</atitle><jtitle>Acta physiologiae plantarum</jtitle><stitle>Acta Physiol Plant</stitle><date>2018-02-01</date><risdate>2018</risdate><volume>40</volume><issue>2</issue><spage>1</spage><epage>11</epage><pages>1-11</pages><artnum>23</artnum><issn>0137-5881</issn><eissn>1861-1664</eissn><abstract>The phloem-unloading pathway of sucrose and mechanism of sugar-to-oil transition are still unknown in Camellia oleifera Fruit. Here, transmission electronic microscopy (TEM) and confocal laser-scanning microscopy (CLSM) were used to observe the cellular structure of vascular bundles and symplastic tracer, carboxyfluorescein (CF), transport in phloem zone. The results showed that sucrose was transported via symplast system in the early and late phases, whereas apoplast system exerted the function in middle stage. Moreover, enzymatic assays showed that acid invertase had a higher activity at the transition stage during the whole fruit development. The cell wall bound invertase (CWI) activity reached the highest at the middle stage of fruit development and the switch in phloem-unloading coincided with fruit developmental phase change and oil accumulation. Correlation analysis showed that the oil accumulation was significantly negatively correlated with content of soluble sugar at P  &lt; 0.05 level. However, the soluble acid invertase (SAI), CWI, and neutral invertase showed a significant positive correlation with oil accumulation at P  &lt; 0.01 level. In summary, our data provide new cytological insights into the transition of unloading transfer between symplasmic and apoplasmic patterns in C. oleifera fruit and suggest that invertases are positively involved in sugar–oil transition process.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s11738-017-2598-z</doi><tpages>11</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0137-5881
ispartof Acta physiologiae plantarum, 2018-02, Vol.40 (2), p.1-11, Article 23
issn 0137-5881
1861-1664
language eng
recordid cdi_proquest_journals_1984769960
source SpringerNature Journals
subjects Accumulation
Acids
Agriculture
Apoplast
Biomedical and Life Sciences
Camellia
Cell walls
Cellular structure
Correlation analysis
Developmental stages
Enzymatic activity
Fruits
Invertase
Life Sciences
Microscopy
Oil
Original Article
Phase transitions
Phloem
Plant Anatomy/Development
Plant Biochemistry
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Scanning microscopy
Sucrose
Sugar
Unloading
title The cellular pathway and enzymatic activity for phloem-unloading transition in developing Camellia oleifera Abel. Fruit
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-05T21%3A19%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20cellular%20pathway%20and%20enzymatic%20activity%20for%20phloem-unloading%20transition%20in%20developing%20Camellia%20oleifera%20Abel.%20Fruit&rft.jtitle=Acta%20physiologiae%20plantarum&rft.au=Wang,%20Xiaoyi&rft.date=2018-02-01&rft.volume=40&rft.issue=2&rft.spage=1&rft.epage=11&rft.pages=1-11&rft.artnum=23&rft.issn=0137-5881&rft.eissn=1861-1664&rft_id=info:doi/10.1007/s11738-017-2598-z&rft_dat=%3Cproquest_cross%3E1984769960%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1984769960&rft_id=info:pmid/&rfr_iscdi=true