The cellular pathway and enzymatic activity for phloem-unloading transition in developing Camellia oleifera Abel. Fruit
The phloem-unloading pathway of sucrose and mechanism of sugar-to-oil transition are still unknown in Camellia oleifera Fruit. Here, transmission electronic microscopy (TEM) and confocal laser-scanning microscopy (CLSM) were used to observe the cellular structure of vascular bundles and symplastic t...
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| Veröffentlicht in: | Acta physiologiae plantarum 2018-02, Vol.40 (2), p.1-11, Article 23 |
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| creator | Wang, Xiaoyi You, Hanli Yuan, Yihang Zhang, Hehua Zhang, Lingyun |
| description | The phloem-unloading pathway of sucrose and mechanism of sugar-to-oil transition are still unknown in
Camellia oleifera
Fruit. Here, transmission electronic microscopy (TEM) and confocal laser-scanning microscopy (CLSM) were used to observe the cellular structure of vascular bundles and symplastic tracer, carboxyfluorescein (CF), transport in phloem zone. The results showed that sucrose was transported via symplast system in the early and late phases, whereas apoplast system exerted the function in middle stage. Moreover, enzymatic assays showed that acid invertase had a higher activity at the transition stage during the whole fruit development. The cell wall bound invertase (CWI) activity reached the highest at the middle stage of fruit development and the switch in phloem-unloading coincided with fruit developmental phase change and oil accumulation. Correlation analysis showed that the oil accumulation was significantly negatively correlated with content of soluble sugar at
P
|
| doi_str_mv | 10.1007/s11738-017-2598-z |
| format | Article |
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Camellia oleifera
Fruit. Here, transmission electronic microscopy (TEM) and confocal laser-scanning microscopy (CLSM) were used to observe the cellular structure of vascular bundles and symplastic tracer, carboxyfluorescein (CF), transport in phloem zone. The results showed that sucrose was transported via symplast system in the early and late phases, whereas apoplast system exerted the function in middle stage. Moreover, enzymatic assays showed that acid invertase had a higher activity at the transition stage during the whole fruit development. The cell wall bound invertase (CWI) activity reached the highest at the middle stage of fruit development and the switch in phloem-unloading coincided with fruit developmental phase change and oil accumulation. Correlation analysis showed that the oil accumulation was significantly negatively correlated with content of soluble sugar at
P
< 0.05 level. However, the soluble acid invertase (SAI), CWI, and neutral invertase showed a significant positive correlation with oil accumulation at
P
< 0.01 level. In summary, our data provide new cytological insights into the transition of unloading transfer between symplasmic and apoplasmic patterns in
C. oleifera
fruit and suggest that invertases are positively involved in sugar–oil transition process.</description><identifier>ISSN: 0137-5881</identifier><identifier>EISSN: 1861-1664</identifier><identifier>DOI: 10.1007/s11738-017-2598-z</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Accumulation ; Acids ; Agriculture ; Apoplast ; Biomedical and Life Sciences ; Camellia ; Cell walls ; Cellular structure ; Correlation analysis ; Developmental stages ; Enzymatic activity ; Fruits ; Invertase ; Life Sciences ; Microscopy ; Oil ; Original Article ; Phase transitions ; Phloem ; Plant Anatomy/Development ; Plant Biochemistry ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Scanning microscopy ; Sucrose ; Sugar ; Unloading</subject><ispartof>Acta physiologiae plantarum, 2018-02, Vol.40 (2), p.1-11, Article 23</ispartof><rights>Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2018</rights><rights>Copyright Springer Science & Business Media 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-2e4c5fa24d7b890c4e09ff951d12acc19b85961b9eadd29084a26c0856fe72723</citedby><cites>FETCH-LOGICAL-c316t-2e4c5fa24d7b890c4e09ff951d12acc19b85961b9eadd29084a26c0856fe72723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11738-017-2598-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11738-017-2598-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,782,786,27931,27932,41495,42564,51326</link.rule.ids></links><search><creatorcontrib>Wang, Xiaoyi</creatorcontrib><creatorcontrib>You, Hanli</creatorcontrib><creatorcontrib>Yuan, Yihang</creatorcontrib><creatorcontrib>Zhang, Hehua</creatorcontrib><creatorcontrib>Zhang, Lingyun</creatorcontrib><title>The cellular pathway and enzymatic activity for phloem-unloading transition in developing Camellia oleifera Abel. Fruit</title><title>Acta physiologiae plantarum</title><addtitle>Acta Physiol Plant</addtitle><description>The phloem-unloading pathway of sucrose and mechanism of sugar-to-oil transition are still unknown in
Camellia oleifera
Fruit. Here, transmission electronic microscopy (TEM) and confocal laser-scanning microscopy (CLSM) were used to observe the cellular structure of vascular bundles and symplastic tracer, carboxyfluorescein (CF), transport in phloem zone. The results showed that sucrose was transported via symplast system in the early and late phases, whereas apoplast system exerted the function in middle stage. Moreover, enzymatic assays showed that acid invertase had a higher activity at the transition stage during the whole fruit development. The cell wall bound invertase (CWI) activity reached the highest at the middle stage of fruit development and the switch in phloem-unloading coincided with fruit developmental phase change and oil accumulation. Correlation analysis showed that the oil accumulation was significantly negatively correlated with content of soluble sugar at
P
< 0.05 level. However, the soluble acid invertase (SAI), CWI, and neutral invertase showed a significant positive correlation with oil accumulation at
P
< 0.01 level. In summary, our data provide new cytological insights into the transition of unloading transfer between symplasmic and apoplasmic patterns in
C. oleifera
fruit and suggest that invertases are positively involved in sugar–oil transition process.</description><subject>Accumulation</subject><subject>Acids</subject><subject>Agriculture</subject><subject>Apoplast</subject><subject>Biomedical and Life Sciences</subject><subject>Camellia</subject><subject>Cell walls</subject><subject>Cellular structure</subject><subject>Correlation analysis</subject><subject>Developmental stages</subject><subject>Enzymatic activity</subject><subject>Fruits</subject><subject>Invertase</subject><subject>Life Sciences</subject><subject>Microscopy</subject><subject>Oil</subject><subject>Original Article</subject><subject>Phase transitions</subject><subject>Phloem</subject><subject>Plant Anatomy/Development</subject><subject>Plant Biochemistry</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Scanning microscopy</subject><subject>Sucrose</subject><subject>Sugar</subject><subject>Unloading</subject><issn>0137-5881</issn><issn>1861-1664</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kD1PIzEURa0VK21g-QF0lqjN-nlmPHaJIj5WQqIJteV43hAjxw62B5T8eiYKxTZbveKee590CLkCfgOc938KQN8oxqFnotOKHX6QBSgJDKRsz8iCQ9OzTin4Rc5LeeO8azopF-RztUHqMIQp2Ex3tm4-7Z7aOFCMh_3WVu-oddV_-LqnY5qRTUi4ZVMMyQ4-vtKabSy--hSpj3TADwxpdwyWdjvvektTQD9itvR2jeGG3ufJ19_k52hDwcvve0Fe7u9Wy0f29Pzwd3n7xFwDsjKBretGK9qhXyvNXYtcj6PuYABhnQO9Vp2WsNZoh0ForlorpOOqkyP2ohfNBbk-7e5yep-wVPOWphznlwa0anupteQzBSfK5VRKxtHsst_avDfAzdGvOfk1s19z9GsOc0ecOmVm4yvmf5b_W_oCw-2AVA</recordid><startdate>20180201</startdate><enddate>20180201</enddate><creator>Wang, Xiaoyi</creator><creator>You, Hanli</creator><creator>Yuan, Yihang</creator><creator>Zhang, Hehua</creator><creator>Zhang, Lingyun</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20180201</creationdate><title>The cellular pathway and enzymatic activity for phloem-unloading transition in developing Camellia oleifera Abel. Fruit</title><author>Wang, Xiaoyi ; You, Hanli ; Yuan, Yihang ; Zhang, Hehua ; Zhang, Lingyun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-2e4c5fa24d7b890c4e09ff951d12acc19b85961b9eadd29084a26c0856fe72723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Accumulation</topic><topic>Acids</topic><topic>Agriculture</topic><topic>Apoplast</topic><topic>Biomedical and Life Sciences</topic><topic>Camellia</topic><topic>Cell walls</topic><topic>Cellular structure</topic><topic>Correlation analysis</topic><topic>Developmental stages</topic><topic>Enzymatic activity</topic><topic>Fruits</topic><topic>Invertase</topic><topic>Life Sciences</topic><topic>Microscopy</topic><topic>Oil</topic><topic>Original Article</topic><topic>Phase transitions</topic><topic>Phloem</topic><topic>Plant Anatomy/Development</topic><topic>Plant Biochemistry</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Scanning microscopy</topic><topic>Sucrose</topic><topic>Sugar</topic><topic>Unloading</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Xiaoyi</creatorcontrib><creatorcontrib>You, Hanli</creatorcontrib><creatorcontrib>Yuan, Yihang</creatorcontrib><creatorcontrib>Zhang, Hehua</creatorcontrib><creatorcontrib>Zhang, Lingyun</creatorcontrib><collection>CrossRef</collection><jtitle>Acta physiologiae plantarum</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Xiaoyi</au><au>You, Hanli</au><au>Yuan, Yihang</au><au>Zhang, Hehua</au><au>Zhang, Lingyun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The cellular pathway and enzymatic activity for phloem-unloading transition in developing Camellia oleifera Abel. Fruit</atitle><jtitle>Acta physiologiae plantarum</jtitle><stitle>Acta Physiol Plant</stitle><date>2018-02-01</date><risdate>2018</risdate><volume>40</volume><issue>2</issue><spage>1</spage><epage>11</epage><pages>1-11</pages><artnum>23</artnum><issn>0137-5881</issn><eissn>1861-1664</eissn><abstract>The phloem-unloading pathway of sucrose and mechanism of sugar-to-oil transition are still unknown in
Camellia oleifera
Fruit. Here, transmission electronic microscopy (TEM) and confocal laser-scanning microscopy (CLSM) were used to observe the cellular structure of vascular bundles and symplastic tracer, carboxyfluorescein (CF), transport in phloem zone. The results showed that sucrose was transported via symplast system in the early and late phases, whereas apoplast system exerted the function in middle stage. Moreover, enzymatic assays showed that acid invertase had a higher activity at the transition stage during the whole fruit development. The cell wall bound invertase (CWI) activity reached the highest at the middle stage of fruit development and the switch in phloem-unloading coincided with fruit developmental phase change and oil accumulation. Correlation analysis showed that the oil accumulation was significantly negatively correlated with content of soluble sugar at
P
< 0.05 level. However, the soluble acid invertase (SAI), CWI, and neutral invertase showed a significant positive correlation with oil accumulation at
P
< 0.01 level. In summary, our data provide new cytological insights into the transition of unloading transfer between symplasmic and apoplasmic patterns in
C. oleifera
fruit and suggest that invertases are positively involved in sugar–oil transition process.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s11738-017-2598-z</doi><tpages>11</tpages></addata></record> |
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| source | SpringerNature Journals |
| subjects | Accumulation Acids Agriculture Apoplast Biomedical and Life Sciences Camellia Cell walls Cellular structure Correlation analysis Developmental stages Enzymatic activity Fruits Invertase Life Sciences Microscopy Oil Original Article Phase transitions Phloem Plant Anatomy/Development Plant Biochemistry Plant Genetics and Genomics Plant Pathology Plant Physiology Scanning microscopy Sucrose Sugar Unloading |
| title | The cellular pathway and enzymatic activity for phloem-unloading transition in developing Camellia oleifera Abel. Fruit |
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