Bioassay-guided isolation of a novel chemoattractant for Ralstonia solanacearum in tomato root exudates

Tomato root exudates were analyzed using a bioassay to detect the chemoattractant for Ralstonia solanacearum . An activated charcoal-adsorbed fraction of root exudates from tomato cultivar Oogata-fukuju had chemoattractant activity for R. solanacearum strain MAFF 730138. The active component, purifi...

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Veröffentlicht in:Journal of general plant pathology : JGPP 2018-01, Vol.84 (1), p.20-26
Hauptverfasser: Hasegawa, Takuya, Okabe, Atsushi, Kato, Yusuke, Ooshiro, Atsushi, Kawaide, Hiroshi, Natsume, Masahiro
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container_title Journal of general plant pathology : JGPP
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creator Hasegawa, Takuya
Okabe, Atsushi
Kato, Yusuke
Ooshiro, Atsushi
Kawaide, Hiroshi
Natsume, Masahiro
description Tomato root exudates were analyzed using a bioassay to detect the chemoattractant for Ralstonia solanacearum . An activated charcoal-adsorbed fraction of root exudates from tomato cultivar Oogata-fukuju had chemoattractant activity for R. solanacearum strain MAFF 730138. The active component, purified using a Sep-Pak C18 cartridge, an activated charcoal column, diol-modified silica gel, and NH 2 -modified silica gel, is a new hydrophobic attractant. The final purified fraction produced a single peak in a diol-modified silica gel HPLC analysis.
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An activated charcoal-adsorbed fraction of root exudates from tomato cultivar Oogata-fukuju had chemoattractant activity for R. solanacearum strain MAFF 730138. The active component, purified using a Sep-Pak C18 cartridge, an activated charcoal column, diol-modified silica gel, and NH 2 -modified silica gel, is a new hydrophobic attractant. 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subjects Activated carbon
Activated charcoal
Agriculture
Bacterial and Phytoplasma Diseases
Bioassays
Biomedical and Life Sciences
Charcoal
Cultivars
Exudates
Exudation
High-performance liquid chromatography
Hydrophobicity
Life Sciences
Liquid chromatography
Microbiology
Plant Pathology
Ralstonia solanacearum
Silica
Silica gel
Silicon dioxide
Tomatoes
title Bioassay-guided isolation of a novel chemoattractant for Ralstonia solanacearum in tomato root exudates
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