Rapid and Sensitive Determination of L-carnitine and Acetyl-L-carnitine in Liquid Milk Samples with Capillary Zone Electrophoresis Using Indirect UV Detection
The purpose of this study was to establish a new capillary zone electrophoresis method with indirect UV detection for determining the concentration of L-carnitine and acetyl-L-carnitine in liquid milk samples. Orthogonal experimental design was applied to arrange and optimize the experimental condit...
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| Veröffentlicht in: | Food analytical methods 2018, Vol.11 (1), p.170-177 |
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| creator | Kong, Yu Yang, Guifang Chen, Shumeng Hou, Zhanwu Du, Xiaomin Li, Hua Kong, Linghong |
| description | The purpose of this study was to establish a new capillary zone electrophoresis method with indirect UV detection for determining the concentration of L-carnitine and acetyl-L-carnitine in liquid milk samples. Orthogonal experimental design was applied to arrange and optimize the experimental conditions. Under selected conditions (using melamine as the additive), both L-carnitine and acetyl-L-carnitine could be detected in 6 min, with satisfied reproducibilities (RSD% of migration times 0.995), recoveries (>90%), and relative low LODs (3.0 and 5.0 μmol/L for L-carnitine and acetyl-L-carnitine, respectively). The proposed method was successfully applied to the assay of nine brands of bovine milk samples and shows potential for analyzing L-carnitine and acetyl-L-carnitine in other biosamples. |
| doi_str_mv | 10.1007/s12161-017-0987-y |
| format | Article |
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> 0.995), recoveries (>90%), and relative low LODs (3.0 and 5.0 μmol/L for L-carnitine and acetyl-L-carnitine, respectively). The proposed method was successfully applied to the assay of nine brands of bovine milk samples and shows potential for analyzing L-carnitine and acetyl-L-carnitine in other biosamples.</description><identifier>ISSN: 1936-9751</identifier><identifier>EISSN: 1936-976X</identifier><identifier>DOI: 10.1007/s12161-017-0987-y</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Acetyl-L-carnitine ; Analytical Chemistry ; Capillary electrophoresis ; Capillary zone ; Carnitine ; Chemistry ; Chemistry and Materials Science ; Chemistry/Food Science ; Cow's milk ; Design optimization ; Electrophoresis ; Experimental design ; Food Science ; Melamine ; Microbiology ; Migration ; Milk ; Ultraviolet radiation</subject><ispartof>Food analytical methods, 2018, Vol.11 (1), p.170-177</ispartof><rights>Springer Science+Business Media, LLC 2017</rights><rights>Copyright Springer Science & Business Media 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-2b5db84724fb4f3f6f0fa49a316dcc924f1c9645e1cc1cef6ab71b781eb1fec53</citedby><cites>FETCH-LOGICAL-c316t-2b5db84724fb4f3f6f0fa49a316dcc924f1c9645e1cc1cef6ab71b781eb1fec53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12161-017-0987-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12161-017-0987-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Kong, Yu</creatorcontrib><creatorcontrib>Yang, Guifang</creatorcontrib><creatorcontrib>Chen, Shumeng</creatorcontrib><creatorcontrib>Hou, Zhanwu</creatorcontrib><creatorcontrib>Du, Xiaomin</creatorcontrib><creatorcontrib>Li, Hua</creatorcontrib><creatorcontrib>Kong, Linghong</creatorcontrib><title>Rapid and Sensitive Determination of L-carnitine and Acetyl-L-carnitine in Liquid Milk Samples with Capillary Zone Electrophoresis Using Indirect UV Detection</title><title>Food analytical methods</title><addtitle>Food Anal. Methods</addtitle><description>The purpose of this study was to establish a new capillary zone electrophoresis method with indirect UV detection for determining the concentration of L-carnitine and acetyl-L-carnitine in liquid milk samples. Orthogonal experimental design was applied to arrange and optimize the experimental conditions. Under selected conditions (using melamine as the additive), both L-carnitine and acetyl-L-carnitine could be detected in 6 min, with satisfied reproducibilities (RSD% of migration times <0.8%), linearities (
r
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> 0.995), recoveries (>90%), and relative low LODs (3.0 and 5.0 μmol/L for L-carnitine and acetyl-L-carnitine, respectively). The proposed method was successfully applied to the assay of nine brands of bovine milk samples and shows potential for analyzing L-carnitine and acetyl-L-carnitine in other biosamples.</description><subject>Acetyl-L-carnitine</subject><subject>Analytical Chemistry</subject><subject>Capillary electrophoresis</subject><subject>Capillary zone</subject><subject>Carnitine</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemistry/Food Science</subject><subject>Cow's milk</subject><subject>Design optimization</subject><subject>Electrophoresis</subject><subject>Experimental design</subject><subject>Food Science</subject><subject>Melamine</subject><subject>Microbiology</subject><subject>Migration</subject><subject>Milk</subject><subject>Ultraviolet radiation</subject><issn>1936-9751</issn><issn>1936-976X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1Ud1OwjAUXowmIvoA3jXxetqzjW67JIhKgjERMcabputOoTi60Q4NL-OzWsAYbrxqc76_c_IFwSXQa6A0vXEQAYOQQhrSPEvDzVHQgTxmYZ6yt-O_fw9OgzPnFpQymkDUCb6fRaNLIkxJJmicbvUnklts0S61Ea2uDakVGYdSWONBgztqX2K7qcLDsTZkrFdr7_Woqw8yEcumQke-dDsnA59RVcJuyHvtqcMKZWvrZl5bdNqRqdNmRkam1NYDZPq6W0Buw8-DEyUqhxe_bzeY3g1fBg_h-Ol-NOj7BWJgbRgVvbLIkjRKVJGoWDFFlUhy4cFSytyPQeYs6SFICRIVE0UKRZoBFqBQ9uJucLX3bWy9WqNr-aJeW-MjOeQZxBDRjHkW7FnS1s5ZVLyxeunv4kD5tga-r4H7Gvi2Br7xmmivcZ5rZmgPnP8V_QDI4Y61</recordid><startdate>2018</startdate><enddate>2018</enddate><creator>Kong, Yu</creator><creator>Yang, Guifang</creator><creator>Chen, Shumeng</creator><creator>Hou, Zhanwu</creator><creator>Du, Xiaomin</creator><creator>Li, Hua</creator><creator>Kong, Linghong</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>2018</creationdate><title>Rapid and Sensitive Determination of L-carnitine and Acetyl-L-carnitine in Liquid Milk Samples with Capillary Zone Electrophoresis Using Indirect UV Detection</title><author>Kong, Yu ; Yang, Guifang ; Chen, Shumeng ; Hou, Zhanwu ; Du, Xiaomin ; Li, Hua ; Kong, Linghong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-2b5db84724fb4f3f6f0fa49a316dcc924f1c9645e1cc1cef6ab71b781eb1fec53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Acetyl-L-carnitine</topic><topic>Analytical Chemistry</topic><topic>Capillary electrophoresis</topic><topic>Capillary zone</topic><topic>Carnitine</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chemistry/Food Science</topic><topic>Cow's milk</topic><topic>Design optimization</topic><topic>Electrophoresis</topic><topic>Experimental design</topic><topic>Food Science</topic><topic>Melamine</topic><topic>Microbiology</topic><topic>Migration</topic><topic>Milk</topic><topic>Ultraviolet radiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kong, Yu</creatorcontrib><creatorcontrib>Yang, Guifang</creatorcontrib><creatorcontrib>Chen, Shumeng</creatorcontrib><creatorcontrib>Hou, Zhanwu</creatorcontrib><creatorcontrib>Du, Xiaomin</creatorcontrib><creatorcontrib>Li, Hua</creatorcontrib><creatorcontrib>Kong, Linghong</creatorcontrib><collection>CrossRef</collection><jtitle>Food analytical methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kong, Yu</au><au>Yang, Guifang</au><au>Chen, Shumeng</au><au>Hou, Zhanwu</au><au>Du, Xiaomin</au><au>Li, Hua</au><au>Kong, Linghong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid and Sensitive Determination of L-carnitine and Acetyl-L-carnitine in Liquid Milk Samples with Capillary Zone Electrophoresis Using Indirect UV Detection</atitle><jtitle>Food analytical methods</jtitle><stitle>Food Anal. Methods</stitle><date>2018</date><risdate>2018</risdate><volume>11</volume><issue>1</issue><spage>170</spage><epage>177</epage><pages>170-177</pages><issn>1936-9751</issn><eissn>1936-976X</eissn><abstract>The purpose of this study was to establish a new capillary zone electrophoresis method with indirect UV detection for determining the concentration of L-carnitine and acetyl-L-carnitine in liquid milk samples. Orthogonal experimental design was applied to arrange and optimize the experimental conditions. Under selected conditions (using melamine as the additive), both L-carnitine and acetyl-L-carnitine could be detected in 6 min, with satisfied reproducibilities (RSD% of migration times <0.8%), linearities (
r
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> 0.995), recoveries (>90%), and relative low LODs (3.0 and 5.0 μmol/L for L-carnitine and acetyl-L-carnitine, respectively). The proposed method was successfully applied to the assay of nine brands of bovine milk samples and shows potential for analyzing L-carnitine and acetyl-L-carnitine in other biosamples.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s12161-017-0987-y</doi><tpages>8</tpages></addata></record> |
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| subjects | Acetyl-L-carnitine Analytical Chemistry Capillary electrophoresis Capillary zone Carnitine Chemistry Chemistry and Materials Science Chemistry/Food Science Cow's milk Design optimization Electrophoresis Experimental design Food Science Melamine Microbiology Migration Milk Ultraviolet radiation |
| title | Rapid and Sensitive Determination of L-carnitine and Acetyl-L-carnitine in Liquid Milk Samples with Capillary Zone Electrophoresis Using Indirect UV Detection |
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