Polyelectrolyte Complex Beads by Novel Two-Step Process for Improved Performance of Viable Whole-Cell Baeyer-Villiger Monoxygenase by Immobilization
A novel immobilization matrix for the entrapment of viable whole-cell Baeyer–Villiger monooxygenase was developed. Viable recombinant Escherichia coli cells overexpressing cyclohexanone monooxygenase were entrapped in polyelectrolyte complex beads prepared by a two-step reaction of oppositely-charge...
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| Veröffentlicht in: | Catalysts 2017-11, Vol.7 (11), p.353 |
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| creator | Krajčovič, Tomáš Bučko, Marek Vikartovská, Alica Lacík, Igor Uhelská, Lucia Chorvát, Dušan Neděla, Vilém Tihlaříková, Eva Gericke, Martin Heinze, Thomas Gemeiner, Peter |
| description | A novel immobilization matrix for the entrapment of viable whole-cell Baeyer–Villiger monooxygenase was developed. Viable recombinant Escherichia coli cells overexpressing cyclohexanone monooxygenase were entrapped in polyelectrolyte complex beads prepared by a two-step reaction of oppositely-charged polymers including highly defined cellulose sulphate. Immobilized cells exhibited higher operational stability than free cells during 10 repeated cycles of Baeyer–Villiger biooxidations of rac-bicyclo[3.2.0]hept-2-en-6-one to the corresponding lactones (1R,5S)-3-oxabicyclo-[3.3.0]oct-6-en-3-one and (1S,5R)-2-oxabicyclo-[3.3.0]oct-6-en-3-one. The morphology of polyelectrolyte complex beads was characterised by environmental scanning electron microscopy; the spatial distribution of polymers in the beads and cell viability were examined using confocal laser scanning microscopy, and the texture was characterised by the mechanical resistance measurements. |
| doi_str_mv | 10.3390/catal7110353 |
| format | Article |
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Viable recombinant Escherichia coli cells overexpressing cyclohexanone monooxygenase were entrapped in polyelectrolyte complex beads prepared by a two-step reaction of oppositely-charged polymers including highly defined cellulose sulphate. Immobilized cells exhibited higher operational stability than free cells during 10 repeated cycles of Baeyer–Villiger biooxidations of rac-bicyclo[3.2.0]hept-2-en-6-one to the corresponding lactones (1R,5S)-3-oxabicyclo-[3.3.0]oct-6-en-3-one and (1S,5R)-2-oxabicyclo-[3.3.0]oct-6-en-3-one. The morphology of polyelectrolyte complex beads was characterised by environmental scanning electron microscopy; the spatial distribution of polymers in the beads and cell viability were examined using confocal laser scanning microscopy, and the texture was characterised by the mechanical resistance measurements.</description><identifier>ISSN: 2073-4344</identifier><identifier>EISSN: 2073-4344</identifier><identifier>DOI: 10.3390/catal7110353</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Beads ; Catalysts ; Chemical reactions ; Cyclohexanone ; E coli ; Electron microscopy ; Entrapment ; Immobilization ; Lactones ; Polyelectrolytes ; Polymers ; Scanning electron microscopy ; Scanning microscopy ; Spatial distribution</subject><ispartof>Catalysts, 2017-11, Vol.7 (11), p.353</ispartof><rights>Copyright MDPI AG 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c301t-9cfd83e1b1a9521e219f2e60d2a11c051a213bacd59c48e88f4d3172fa913f823</citedby><cites>FETCH-LOGICAL-c301t-9cfd83e1b1a9521e219f2e60d2a11c051a213bacd59c48e88f4d3172fa913f823</cites><orcidid>0000-0001-6029-5435</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Krajčovič, Tomáš</creatorcontrib><creatorcontrib>Bučko, Marek</creatorcontrib><creatorcontrib>Vikartovská, Alica</creatorcontrib><creatorcontrib>Lacík, Igor</creatorcontrib><creatorcontrib>Uhelská, Lucia</creatorcontrib><creatorcontrib>Chorvát, Dušan</creatorcontrib><creatorcontrib>Neděla, Vilém</creatorcontrib><creatorcontrib>Tihlaříková, Eva</creatorcontrib><creatorcontrib>Gericke, Martin</creatorcontrib><creatorcontrib>Heinze, Thomas</creatorcontrib><creatorcontrib>Gemeiner, Peter</creatorcontrib><title>Polyelectrolyte Complex Beads by Novel Two-Step Process for Improved Performance of Viable Whole-Cell Baeyer-Villiger Monoxygenase by Immobilization</title><title>Catalysts</title><description>A novel immobilization matrix for the entrapment of viable whole-cell Baeyer–Villiger monooxygenase was developed. Viable recombinant Escherichia coli cells overexpressing cyclohexanone monooxygenase were entrapped in polyelectrolyte complex beads prepared by a two-step reaction of oppositely-charged polymers including highly defined cellulose sulphate. Immobilized cells exhibited higher operational stability than free cells during 10 repeated cycles of Baeyer–Villiger biooxidations of rac-bicyclo[3.2.0]hept-2-en-6-one to the corresponding lactones (1R,5S)-3-oxabicyclo-[3.3.0]oct-6-en-3-one and (1S,5R)-2-oxabicyclo-[3.3.0]oct-6-en-3-one. 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Bučko, Marek ; Vikartovská, Alica ; Lacík, Igor ; Uhelská, Lucia ; Chorvát, Dušan ; Neděla, Vilém ; Tihlaříková, Eva ; Gericke, Martin ; Heinze, Thomas ; Gemeiner, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c301t-9cfd83e1b1a9521e219f2e60d2a11c051a213bacd59c48e88f4d3172fa913f823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Beads</topic><topic>Catalysts</topic><topic>Chemical reactions</topic><topic>Cyclohexanone</topic><topic>E coli</topic><topic>Electron microscopy</topic><topic>Entrapment</topic><topic>Immobilization</topic><topic>Lactones</topic><topic>Polyelectrolytes</topic><topic>Polymers</topic><topic>Scanning electron microscopy</topic><topic>Scanning microscopy</topic><topic>Spatial distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Krajčovič, Tomáš</creatorcontrib><creatorcontrib>Bučko, Marek</creatorcontrib><creatorcontrib>Vikartovská, Alica</creatorcontrib><creatorcontrib>Lacík, Igor</creatorcontrib><creatorcontrib>Uhelská, Lucia</creatorcontrib><creatorcontrib>Chorvát, Dušan</creatorcontrib><creatorcontrib>Neděla, Vilém</creatorcontrib><creatorcontrib>Tihlaříková, Eva</creatorcontrib><creatorcontrib>Gericke, Martin</creatorcontrib><creatorcontrib>Heinze, Thomas</creatorcontrib><creatorcontrib>Gemeiner, Peter</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>Materials Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Catalysts</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Krajčovič, Tomáš</au><au>Bučko, Marek</au><au>Vikartovská, Alica</au><au>Lacík, Igor</au><au>Uhelská, Lucia</au><au>Chorvát, Dušan</au><au>Neděla, Vilém</au><au>Tihlaříková, Eva</au><au>Gericke, Martin</au><au>Heinze, Thomas</au><au>Gemeiner, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polyelectrolyte Complex Beads by Novel Two-Step Process for Improved Performance of Viable Whole-Cell Baeyer-Villiger Monoxygenase by Immobilization</atitle><jtitle>Catalysts</jtitle><date>2017-11-21</date><risdate>2017</risdate><volume>7</volume><issue>11</issue><spage>353</spage><pages>353-</pages><issn>2073-4344</issn><eissn>2073-4344</eissn><abstract>A novel immobilization matrix for the entrapment of viable whole-cell Baeyer–Villiger monooxygenase was developed. Viable recombinant Escherichia coli cells overexpressing cyclohexanone monooxygenase were entrapped in polyelectrolyte complex beads prepared by a two-step reaction of oppositely-charged polymers including highly defined cellulose sulphate. Immobilized cells exhibited higher operational stability than free cells during 10 repeated cycles of Baeyer–Villiger biooxidations of rac-bicyclo[3.2.0]hept-2-en-6-one to the corresponding lactones (1R,5S)-3-oxabicyclo-[3.3.0]oct-6-en-3-one and (1S,5R)-2-oxabicyclo-[3.3.0]oct-6-en-3-one. 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| source | MDPI - Multidisciplinary Digital Publishing Institute; EZB-FREE-00999 freely available EZB journals |
| subjects | Beads Catalysts Chemical reactions Cyclohexanone E coli Electron microscopy Entrapment Immobilization Lactones Polyelectrolytes Polymers Scanning electron microscopy Scanning microscopy Spatial distribution |
| title | Polyelectrolyte Complex Beads by Novel Two-Step Process for Improved Performance of Viable Whole-Cell Baeyer-Villiger Monoxygenase by Immobilization |
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