AGROBACTERIUM-MEDIATED TRANSFORMATION OF THE APPLE ROOTSTOCK MALUS MICROMALUS MAKINO WITH THE ROLC GENE

Malus micromalus Makino was genetically engineered with the rolC gene via Agrobacterium-mediated transformation. The antibiotics carbenicillin, hygromycin, and kanamycin (Km) inhibited shoot regeneration from in vitro leaf explants. The leaf segments were infected with Agrobacterium tumefaciens stra...

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Veröffentlicht in:	In vitro cellular & developmental biology. Plant 2006-11, Vol.42 (6), p.491-497
Hauptverfasser:	ZHANG, ZHEN, SUN, AIJUN, CONG, YU, SHENG, BINGCHENG, YAO, QUANHONG, CHENG, ZONG-MING
Format:	Artikel
Sprache:	eng
Schlagworte:	Agrobacterium Agrobacterium radiobacter Antibiotics Apples auxins Bacteria BIOTECHNOLOGY/GENETIC TRANSFORMATION/FUNCTIONAL GENOMICS carbenicillin Cultivars dwarf apple rootstock Efficiency explants Forest regeneration Genes genetic transformation Height Internodes kanamycin kanamycin kinase Leaves Malus micromalus Plant roots Plants Plasmids Polymerase chain reaction rooting Rootstocks shoots Southern blotting Transgenic plants
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