Lipoic Acid—A Unique Plant Transformation Enhancer
Including lipoic acid (LA) in culture media during Agrobacterium transformation processes of four crop species has significantly improved the transformation methods of the crops, even for previously recalcitrant genotypes. Plant transformation efficiency of soybean was significantly increased from 0...
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| Veröffentlicht in: | In vitro cellular & developmental biology. Plant 2009-11, Vol.45 (6), p.630-638 |
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| container_title | In vitro cellular & developmental biology. Plant |
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| creator | Dan, Yinghui Armstrong, Charles L. Dong, Jimmy Feng, Xiaorong Fry, Joyce E. Keithly, Greg E. Martinell, Brian J. Roberts, Gail A. Smith, Lori A. Tan, Lalaine J. Duncan, David R. |
| description | Including lipoic acid (LA) in culture media during Agrobacterium transformation processes of four crop species has significantly improved the transformation methods of the crops, even for previously recalcitrant genotypes. Plant transformation efficiency of soybean was significantly increased from 0.6% to 3.7% and tomato from 29.8% to 87.0%. Transformation efficiency was doubled from 2.8% to 5.7% in wheat. The frequency of glyphosateresistant embryos had a significant increase from 41.4% to 61.2% in cotton. Regeneration of non-transgenic shoots under selection ("shoot escapes") was significantly reduced in tomato from 91.5% to 46.2% while in soybean from 92.0% to 72.0% under optimal conditions. This study also demonstrated that the increase of transformation efficiency in tomato was accompanied by as much as a significant 2-fold reduction in severity of browning of Agrobacterium-infected plant tissues and up to a significant 3-fold increase in the percentage of explants with a high level of transient gene expression. LA application in plant transformation has enabled the resolution of three common problems in plant transformation: browning or necrosis of the transformed cells or tissues, difficulty in regenerating transformed cells or tissues, and shoot escapes, which severely limit the number of transgenic plants that can be regenerated. |
| doi_str_mv | 10.1007/s11627-009-9227-5 |
| format | Article |
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Plant transformation efficiency of soybean was significantly increased from 0.6% to 3.7% and tomato from 29.8% to 87.0%. Transformation efficiency was doubled from 2.8% to 5.7% in wheat. The frequency of glyphosateresistant embryos had a significant increase from 41.4% to 61.2% in cotton. Regeneration of non-transgenic shoots under selection ("shoot escapes") was significantly reduced in tomato from 91.5% to 46.2% while in soybean from 92.0% to 72.0% under optimal conditions. This study also demonstrated that the increase of transformation efficiency in tomato was accompanied by as much as a significant 2-fold reduction in severity of browning of Agrobacterium-infected plant tissues and up to a significant 3-fold increase in the percentage of explants with a high level of transient gene expression. LA application in plant transformation has enabled the resolution of three common problems in plant transformation: browning or necrosis of the transformed cells or tissues, difficulty in regenerating transformed cells or tissues, and shoot escapes, which severely limit the number of transgenic plants that can be regenerated.</description><identifier>ISSN: 1054-5476</identifier><identifier>EISSN: 1475-2689</identifier><identifier>DOI: 10.1007/s11627-009-9227-5</identifier><language>eng</language><publisher>New York: Springer</publisher><subject>Agricultural biotechnology ; Agrobacterium ; Antioxidants ; Biomedical and Life Sciences ; Biotechnology ; Cell Biology ; Culture media ; Developmental Biology ; Flowers & plants ; Genomics ; Genotypes ; Life Sciences ; Necrosis ; Plant Breeding/Biotechnology ; Plant cells ; Plant Genetics and Genomics ; Plant propagation ; Plant Sciences ; Plant tissues ; Plants ; Rice ; Soybeans ; Tomatoes ; Transgenic plants</subject><ispartof>In vitro cellular & developmental biology. Plant, 2009-11, Vol.45 (6), p.630-638</ispartof><rights>Copyright 2009 Society for In Vitro Biology</rights><rights>The Society for In Vitro Biology 2009</rights><rights>Copyright Society for In Vitro Biology Nov/Dec 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c337t-1877cecae7f1062ad41e8d4b367f21ae7323f10c6d5ccda5c817ae79d8918f0e3</citedby><cites>FETCH-LOGICAL-c337t-1877cecae7f1062ad41e8d4b367f21ae7323f10c6d5ccda5c817ae79d8918f0e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/25623022$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/25623022$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27903,27904,41467,42536,51297,57995,58228</link.rule.ids></links><search><creatorcontrib>Dan, Yinghui</creatorcontrib><creatorcontrib>Armstrong, Charles L.</creatorcontrib><creatorcontrib>Dong, Jimmy</creatorcontrib><creatorcontrib>Feng, Xiaorong</creatorcontrib><creatorcontrib>Fry, Joyce E.</creatorcontrib><creatorcontrib>Keithly, Greg E.</creatorcontrib><creatorcontrib>Martinell, Brian J.</creatorcontrib><creatorcontrib>Roberts, Gail A.</creatorcontrib><creatorcontrib>Smith, Lori A.</creatorcontrib><creatorcontrib>Tan, Lalaine J.</creatorcontrib><creatorcontrib>Duncan, David R.</creatorcontrib><title>Lipoic Acid—A Unique Plant Transformation Enhancer</title><title>In vitro cellular & developmental biology. Plant</title><addtitle>In Vitro Cell.Dev.Biol.-Plant</addtitle><description>Including lipoic acid (LA) in culture media during Agrobacterium transformation processes of four crop species has significantly improved the transformation methods of the crops, even for previously recalcitrant genotypes. Plant transformation efficiency of soybean was significantly increased from 0.6% to 3.7% and tomato from 29.8% to 87.0%. Transformation efficiency was doubled from 2.8% to 5.7% in wheat. The frequency of glyphosateresistant embryos had a significant increase from 41.4% to 61.2% in cotton. Regeneration of non-transgenic shoots under selection ("shoot escapes") was significantly reduced in tomato from 91.5% to 46.2% while in soybean from 92.0% to 72.0% under optimal conditions. This study also demonstrated that the increase of transformation efficiency in tomato was accompanied by as much as a significant 2-fold reduction in severity of browning of Agrobacterium-infected plant tissues and up to a significant 3-fold increase in the percentage of explants with a high level of transient gene expression. LA application in plant transformation has enabled the resolution of three common problems in plant transformation: browning or necrosis of the transformed cells or tissues, difficulty in regenerating transformed cells or tissues, and shoot escapes, which severely limit the number of transgenic plants that can be regenerated.</description><subject>Agricultural biotechnology</subject><subject>Agrobacterium</subject><subject>Antioxidants</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cell Biology</subject><subject>Culture media</subject><subject>Developmental Biology</subject><subject>Flowers & plants</subject><subject>Genomics</subject><subject>Genotypes</subject><subject>Life Sciences</subject><subject>Necrosis</subject><subject>Plant Breeding/Biotechnology</subject><subject>Plant cells</subject><subject>Plant Genetics and Genomics</subject><subject>Plant propagation</subject><subject>Plant Sciences</subject><subject>Plant tissues</subject><subject>Plants</subject><subject>Rice</subject><subject>Soybeans</subject><subject>Tomatoes</subject><subject>Transgenic plants</subject><issn>1054-5476</issn><issn>1475-2689</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9UM1KAzEQDqJgrT6AB2HxHp3J7-6xlFqFgh7ac4jZrG5pszXZHrz5ED6hT2LKinjyNB8z38_wEXKJcIMA-jYhKqYpQEUrloE8IiMUWlKmyuo4Y5CCSqHVKTlLaQ0ACKhHRCzaXde6YuLa-uvjc1KsQvu298XTxoa-WEYbUtPFre3bLhSz8GqD8_GcnDR2k_zFzxyT1d1sOb2ni8f5w3SyoI5z3VMstXbeWa8bBMVsLdCXtXjmSjcM85ozni9O1dK52kpXos7bqi4rLBvwfEyuB99d7PJTqTfrbh9DjjRYaQECVJVJOJBc7FKKvjG72G5tfDcI5tCNGboxuRtz6MbIrGGDJmVuePHxj_E_oqtBtE59F39TmFSMA2P8G8pTcEg</recordid><startdate>200911</startdate><enddate>200911</enddate><creator>Dan, Yinghui</creator><creator>Armstrong, Charles L.</creator><creator>Dong, Jimmy</creator><creator>Feng, Xiaorong</creator><creator>Fry, Joyce E.</creator><creator>Keithly, Greg E.</creator><creator>Martinell, Brian J.</creator><creator>Roberts, Gail A.</creator><creator>Smith, Lori A.</creator><creator>Tan, Lalaine J.</creator><creator>Duncan, David R.</creator><general>Springer</general><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>4U-</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>S0X</scope></search><sort><creationdate>200911</creationdate><title>Lipoic Acid—A Unique Plant Transformation Enhancer</title><author>Dan, Yinghui ; Armstrong, Charles L. ; Dong, Jimmy ; Feng, Xiaorong ; Fry, Joyce E. ; Keithly, Greg E. ; Martinell, Brian J. ; Roberts, Gail A. ; Smith, Lori A. ; Tan, Lalaine J. ; Duncan, David R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c337t-1877cecae7f1062ad41e8d4b367f21ae7323f10c6d5ccda5c817ae79d8918f0e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Agricultural biotechnology</topic><topic>Agrobacterium</topic><topic>Antioxidants</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cell Biology</topic><topic>Culture media</topic><topic>Developmental Biology</topic><topic>Flowers & plants</topic><topic>Genomics</topic><topic>Genotypes</topic><topic>Life Sciences</topic><topic>Necrosis</topic><topic>Plant Breeding/Biotechnology</topic><topic>Plant cells</topic><topic>Plant Genetics and Genomics</topic><topic>Plant propagation</topic><topic>Plant Sciences</topic><topic>Plant tissues</topic><topic>Plants</topic><topic>Rice</topic><topic>Soybeans</topic><topic>Tomatoes</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dan, Yinghui</creatorcontrib><creatorcontrib>Armstrong, Charles L.</creatorcontrib><creatorcontrib>Dong, Jimmy</creatorcontrib><creatorcontrib>Feng, Xiaorong</creatorcontrib><creatorcontrib>Fry, Joyce E.</creatorcontrib><creatorcontrib>Keithly, Greg E.</creatorcontrib><creatorcontrib>Martinell, Brian J.</creatorcontrib><creatorcontrib>Roberts, Gail A.</creatorcontrib><creatorcontrib>Smith, Lori A.</creatorcontrib><creatorcontrib>Tan, Lalaine J.</creatorcontrib><creatorcontrib>Duncan, David R.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><jtitle>In vitro cellular & developmental biology. Plant</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dan, Yinghui</au><au>Armstrong, Charles L.</au><au>Dong, Jimmy</au><au>Feng, Xiaorong</au><au>Fry, Joyce E.</au><au>Keithly, Greg E.</au><au>Martinell, Brian J.</au><au>Roberts, Gail A.</au><au>Smith, Lori A.</au><au>Tan, Lalaine J.</au><au>Duncan, David R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lipoic Acid—A Unique Plant Transformation Enhancer</atitle><jtitle>In vitro cellular & developmental biology. 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This study also demonstrated that the increase of transformation efficiency in tomato was accompanied by as much as a significant 2-fold reduction in severity of browning of Agrobacterium-infected plant tissues and up to a significant 3-fold increase in the percentage of explants with a high level of transient gene expression. LA application in plant transformation has enabled the resolution of three common problems in plant transformation: browning or necrosis of the transformed cells or tissues, difficulty in regenerating transformed cells or tissues, and shoot escapes, which severely limit the number of transgenic plants that can be regenerated.</abstract><cop>New York</cop><pub>Springer</pub><doi>10.1007/s11627-009-9227-5</doi><tpages>9</tpages></addata></record> |
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| ispartof | In vitro cellular & developmental biology. Plant, 2009-11, Vol.45 (6), p.630-638 |
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| subjects | Agricultural biotechnology Agrobacterium Antioxidants Biomedical and Life Sciences Biotechnology Cell Biology Culture media Developmental Biology Flowers & plants Genomics Genotypes Life Sciences Necrosis Plant Breeding/Biotechnology Plant cells Plant Genetics and Genomics Plant propagation Plant Sciences Plant tissues Plants Rice Soybeans Tomatoes Transgenic plants |
| title | Lipoic Acid—A Unique Plant Transformation Enhancer |
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