A technology platform for digital nucleic acid diagnostics at the point of care
The combination of digital amplification and centrifugal microfluidics can enable quantitative and fast diagnostics at the point of care (PoC). The new unit operation of centrifugal step emulsification allows high throughput droplet generation. Different methods for digital nucleic acid analysis, in...
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| Veröffentlicht in: | Laboratoriumsmedizin 2017-10, Vol.41 (5), p.245-249 |
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| container_title | Laboratoriumsmedizin |
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| creator | Borst, Nadine Schuler, Friedrich Wadle, Simon Schulz, Martin Specht, Mara Li, Jia Becherer, Lisa Trotter, Martin Rodríguez-Martínez, Ana Belén Paust, Nils Zengerle, Roland Stetten, Felix von |
| description | The combination of digital amplification and centrifugal microfluidics can enable quantitative and fast diagnostics at the point of care (PoC). The new unit operation of centrifugal step emulsification allows high throughput droplet generation. Different methods for digital nucleic acid analysis, including PCR, recombinase polymerase amplification (RPA) and loop mediated isothermal amplification (LAMP), have already been demonstrated. Our novel approach of integrated sample-to-answer analysis is introduced, and examples for the detection of HIV and single cell analysis of antibiotic resistant bacteria are presented. Next to these LabDisk based systems, a microfluidic cartridge termed DropChip allows for digital amplification using only commercially available laboratory devices. |
| doi_str_mv | 10.1515/labmed-2017-0098 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 0342-3026 |
| ispartof | Laboratoriumsmedizin, 2017-10, Vol.41 (5), p.245-249 |
| issn | 0342-3026 1439-0477 |
| language | eng |
| recordid | cdi_proquest_journals_1971760159 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Acids Antibiotic resistance Antibiotics Bacteria digital nucleic acid diagnostics digitale Nukleinsäurediagnostik Emulsification lab-on-a-chip Microfluidics Nucleic acids point-of-care testing (POCT) Recombinase |
| title | A technology platform for digital nucleic acid diagnostics at the point of care |
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