Magnetic Fe3S4 nanoparticles with peroxidase-like activity, and their use in a photometric enzymatic glucose assay
Greigite magnetic nanoparticles (Fe 3 S 4 -MNPs) were prepared and reveal a peroxidase-like activity. Kinetic studies revealed a pseudo-enzymatic activity that is much higher than that of other magnetic nanomaterial-based enzyme mimetics. This finding was exploited to design a photometric enzymatic...
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| Veröffentlicht in: | Mikrochimica acta (1966) 2016-02, Vol.183 (2), p.625-631 |
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| container_title | Mikrochimica acta (1966) |
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| creator | Ding, Caiping Yan, Yinghan Xiang, Dongshan Zhang, Cuiling Xian, Yuezhong |
| description | Greigite magnetic nanoparticles (Fe
3
S
4
-MNPs) were prepared and reveal a peroxidase-like activity. Kinetic studies revealed a pseudo-enzymatic activity that is much higher than that of other magnetic nanomaterial-based enzyme mimetics. This finding was exploited to design a photometric enzymatic glucose assay based on the formation of H
2
O
2
during enzymatic oxidation of glucose by glucose oxidase, and the formation of a blue product from an enzyme substrate that is catalytically oxidized by H
2
O
2
in the presence of Fe
3
S
4
-MNPs. Glucose can be detected in the 2 to 100 μM concentration range, and the low detection limit is 0.16 μM. The method was applied to quantify glucose in human serum. In our perception, this enzyme mimetic has a large potential in that it may be used in other oxidase based assays, but also in ELISAs.
Graphic Abstract
Fe
3
S
4
magnetic nanoparticles (MNPs) are shown to act as peroxidase mimetics and this was used to design a glucose oxidase (GOx) based glucose assay where the H
2
O
2
formed during oxidation of glucose oxidizes tetramethylbenzidine (TMB) to give a blue product which can be quantified by photometry. |
| doi_str_mv | 10.1007/s00604-015-1690-6 |
| format | Article |
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3
S
4
-MNPs) were prepared and reveal a peroxidase-like activity. Kinetic studies revealed a pseudo-enzymatic activity that is much higher than that of other magnetic nanomaterial-based enzyme mimetics. This finding was exploited to design a photometric enzymatic glucose assay based on the formation of H
2
O
2
during enzymatic oxidation of glucose by glucose oxidase, and the formation of a blue product from an enzyme substrate that is catalytically oxidized by H
2
O
2
in the presence of Fe
3
S
4
-MNPs. Glucose can be detected in the 2 to 100 μM concentration range, and the low detection limit is 0.16 μM. The method was applied to quantify glucose in human serum. In our perception, this enzyme mimetic has a large potential in that it may be used in other oxidase based assays, but also in ELISAs.
Graphic Abstract
Fe
3
S
4
magnetic nanoparticles (MNPs) are shown to act as peroxidase mimetics and this was used to design a glucose oxidase (GOx) based glucose assay where the H
2
O
2
formed during oxidation of glucose oxidizes tetramethylbenzidine (TMB) to give a blue product which can be quantified by photometry.</description><identifier>ISSN: 0026-3672</identifier><identifier>EISSN: 1436-5073</identifier><identifier>DOI: 10.1007/s00604-015-1690-6</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Analytical Chemistry ; Assaying ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Enzymes ; Glucose oxidase ; Hydrogen peroxide ; Microengineering ; Nanochemistry ; Nanomaterials ; Nanoparticles ; Nanotechnology ; Original Paper ; Oxidation ; Peroxidase ; Photometry</subject><ispartof>Mikrochimica acta (1966), 2016-02, Vol.183 (2), p.625-631</ispartof><rights>Springer-Verlag Wien 2015</rights><rights>Microchimica Acta is a copyright of Springer, (2015). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-fbbac56dacca25aa352bd66af547451c8dbd32e290965edd695f422c27fe15713</citedby><cites>FETCH-LOGICAL-c386t-fbbac56dacca25aa352bd66af547451c8dbd32e290965edd695f422c27fe15713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00604-015-1690-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00604-015-1690-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids></links><search><creatorcontrib>Ding, Caiping</creatorcontrib><creatorcontrib>Yan, Yinghan</creatorcontrib><creatorcontrib>Xiang, Dongshan</creatorcontrib><creatorcontrib>Zhang, Cuiling</creatorcontrib><creatorcontrib>Xian, Yuezhong</creatorcontrib><title>Magnetic Fe3S4 nanoparticles with peroxidase-like activity, and their use in a photometric enzymatic glucose assay</title><title>Mikrochimica acta (1966)</title><addtitle>Microchim Acta</addtitle><description>Greigite magnetic nanoparticles (Fe
3
S
4
-MNPs) were prepared and reveal a peroxidase-like activity. Kinetic studies revealed a pseudo-enzymatic activity that is much higher than that of other magnetic nanomaterial-based enzyme mimetics. This finding was exploited to design a photometric enzymatic glucose assay based on the formation of H
2
O
2
during enzymatic oxidation of glucose by glucose oxidase, and the formation of a blue product from an enzyme substrate that is catalytically oxidized by H
2
O
2
in the presence of Fe
3
S
4
-MNPs. Glucose can be detected in the 2 to 100 μM concentration range, and the low detection limit is 0.16 μM. The method was applied to quantify glucose in human serum. In our perception, this enzyme mimetic has a large potential in that it may be used in other oxidase based assays, but also in ELISAs.
Graphic Abstract
Fe
3
S
4
magnetic nanoparticles (MNPs) are shown to act as peroxidase mimetics and this was used to design a glucose oxidase (GOx) based glucose assay where the H
2
O
2
formed during oxidation of glucose oxidizes tetramethylbenzidine (TMB) to give a blue product which can be quantified by photometry.</description><subject>Analytical Chemistry</subject><subject>Assaying</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Enzymes</subject><subject>Glucose oxidase</subject><subject>Hydrogen peroxide</subject><subject>Microengineering</subject><subject>Nanochemistry</subject><subject>Nanomaterials</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>Original Paper</subject><subject>Oxidation</subject><subject>Peroxidase</subject><subject>Photometry</subject><issn>0026-3672</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp1kEtLAzEURoMoWB8_wF3ArdE8Jnc6SxFfUHGhrsNt5k4bbWfGJFXrr3dKXbhxFbic7wQOYydKnispy4skJchCSGWFgkoK2GEjVRgQVpZml42k1CAMlHqfHaT0KqUqQRcjFh9w1lIOnt-QeSp4i23XYxwOC0r8M-Q57yl2X6HGRGIR3oijz-Ej5PUZx7bmeU4h8lUiHlqOvJ93uVtSjoOR2u_1Ejfu2WLluwHBlHB9xPYaXCQ6_n0P2cvN9fPVnZg83t5fXU6EN2PIoplO0Vuo0XvUFtFYPa0BsLFFWVjlx_W0Npp0JSuwVNdQ2abQ2uuyIWVLZQ7Z6dbbx-59RSm7124V2-FLpyoYW7BQmoFSW8rHLqVIjetjWGJcOyXdJq3bpnVDWrdJ62DY6O0mDWw7o_jH_O_oBwdZfbA</recordid><startdate>20160201</startdate><enddate>20160201</enddate><creator>Ding, Caiping</creator><creator>Yan, Yinghan</creator><creator>Xiang, Dongshan</creator><creator>Zhang, Cuiling</creator><creator>Xian, Yuezhong</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>M0S</scope><scope>M1P</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20160201</creationdate><title>Magnetic Fe3S4 nanoparticles with peroxidase-like activity, and their use in a photometric enzymatic glucose assay</title><author>Ding, Caiping ; Yan, Yinghan ; Xiang, Dongshan ; Zhang, Cuiling ; Xian, Yuezhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-fbbac56dacca25aa352bd66af547451c8dbd32e290965edd695f422c27fe15713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Analytical Chemistry</topic><topic>Assaying</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Enzymes</topic><topic>Glucose oxidase</topic><topic>Hydrogen peroxide</topic><topic>Microengineering</topic><topic>Nanochemistry</topic><topic>Nanomaterials</topic><topic>Nanoparticles</topic><topic>Nanotechnology</topic><topic>Original Paper</topic><topic>Oxidation</topic><topic>Peroxidase</topic><topic>Photometry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ding, Caiping</creatorcontrib><creatorcontrib>Yan, Yinghan</creatorcontrib><creatorcontrib>Xiang, Dongshan</creatorcontrib><creatorcontrib>Zhang, Cuiling</creatorcontrib><creatorcontrib>Xian, Yuezhong</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ding, Caiping</au><au>Yan, Yinghan</au><au>Xiang, Dongshan</au><au>Zhang, Cuiling</au><au>Xian, Yuezhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Magnetic Fe3S4 nanoparticles with peroxidase-like activity, and their use in a photometric enzymatic glucose assay</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><date>2016-02-01</date><risdate>2016</risdate><volume>183</volume><issue>2</issue><spage>625</spage><epage>631</epage><pages>625-631</pages><issn>0026-3672</issn><eissn>1436-5073</eissn><abstract>Greigite magnetic nanoparticles (Fe
3
S
4
-MNPs) were prepared and reveal a peroxidase-like activity. Kinetic studies revealed a pseudo-enzymatic activity that is much higher than that of other magnetic nanomaterial-based enzyme mimetics. This finding was exploited to design a photometric enzymatic glucose assay based on the formation of H
2
O
2
during enzymatic oxidation of glucose by glucose oxidase, and the formation of a blue product from an enzyme substrate that is catalytically oxidized by H
2
O
2
in the presence of Fe
3
S
4
-MNPs. Glucose can be detected in the 2 to 100 μM concentration range, and the low detection limit is 0.16 μM. The method was applied to quantify glucose in human serum. In our perception, this enzyme mimetic has a large potential in that it may be used in other oxidase based assays, but also in ELISAs.
Graphic Abstract
Fe
3
S
4
magnetic nanoparticles (MNPs) are shown to act as peroxidase mimetics and this was used to design a glucose oxidase (GOx) based glucose assay where the H
2
O
2
formed during oxidation of glucose oxidizes tetramethylbenzidine (TMB) to give a blue product which can be quantified by photometry.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><doi>10.1007/s00604-015-1690-6</doi><tpages>7</tpages></addata></record> |
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| issn | 0026-3672 1436-5073 |
| language | eng |
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| subjects | Analytical Chemistry Assaying Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Enzymes Glucose oxidase Hydrogen peroxide Microengineering Nanochemistry Nanomaterials Nanoparticles Nanotechnology Original Paper Oxidation Peroxidase Photometry |
| title | Magnetic Fe3S4 nanoparticles with peroxidase-like activity, and their use in a photometric enzymatic glucose assay |
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