Amperometric biosensor for microRNA based on the use of tetrahedral DNA nanostructure probes and guanine nanowire amplification
MicroRNAs are endogenous noncoding RNAs that play critical roles in biological processes and can be considered as molecular markers for early diagnosis and pathogenesis of diseases. The authors describe a highly sensitive electrochemical biosensor for microRNA that is based on the use of tetrahedral...
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| Veröffentlicht in: | Mikrochimica acta (1966) 2017-08, Vol.184 (8), p.2597-2604 |
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| container_title | Mikrochimica acta (1966) |
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| creator | Huang, Yan Li Mo, Shi Gao, Zhong Feng Chen, Jing Rong Lei, Jing Lei Luo, Hong Qun Li, Nian Bing |
| description | MicroRNAs are endogenous noncoding RNAs that play critical roles in biological processes and can be considered as molecular markers for early diagnosis and pathogenesis of diseases. The authors describe a highly sensitive electrochemical biosensor for microRNA that is based on the use of tetrahedral DNA nanostructure probes and guanine nanowire amplification. The DNA tetrahedral probe is self-assembled on a gold electrode and enhances reactivity, accessibility, and molecular recognition efficiency. Combined with the tetrahedral probe, the guanine nanowire amplifies the signal and improves the analytical performance of the biosensor. Operated best at a voltage of typically 150 mV (vs. Ag/AgCl), the sensor has a linear response to the logarithmic microRNA concentration in the 500 f. to 10 nM range, with a 176 f. detection limit. It is highly selective and can be applied to real samples. It is concluded that this strategy has a good potential with respect to the determination of microRNA in clinical diagnosis and in biological research.
Graphical abstract
Schematic of a tetrahedral DNA nanostructure-based amperometric biosensor coupled to guanine nanowire amplification for analysis of microRNA-21. This strategy is highly selective and also performs well for the detection of microRNA levels of breast cancer patients. |
| doi_str_mv | 10.1007/s00604-017-2246-8 |
| format | Article |
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Graphical abstract
Schematic of a tetrahedral DNA nanostructure-based amperometric biosensor coupled to guanine nanowire amplification for analysis of microRNA-21. This strategy is highly selective and also performs well for the detection of microRNA levels of breast cancer patients.</description><identifier>ISSN: 0026-3672</identifier><identifier>EISSN: 1436-5073</identifier><identifier>DOI: 10.1007/s00604-017-2246-8</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Accessibility ; Amplification ; Analytical Chemistry ; Biological activity ; Biosensors ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Deoxyribonucleic acid ; Detectors ; Diagnosis ; DNA ; Electric potential ; Electrical measurement ; Gold ; Guanine ; Markers ; Microengineering ; MicroRNA ; MicroRNAs ; Nanochemistry ; Nanostructure ; Nanotechnology ; Nanowires ; Original Paper ; Pathogenesis ; Ribonucleic acid ; RNA</subject><ispartof>Mikrochimica acta (1966), 2017-08, Vol.184 (8), p.2597-2604</ispartof><rights>Springer-Verlag Wien 2017</rights><rights>COPYRIGHT 2017 Springer</rights><rights>Copyright Springer Science & Business Media 2017</rights><rights>Microchimica Acta is a copyright of Springer, (2017). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c449t-ed9bb3a4be145dd5558034b40d5408ff959ecb9849ca6e7f94fbc2b2b8de3da33</citedby><cites>FETCH-LOGICAL-c449t-ed9bb3a4be145dd5558034b40d5408ff959ecb9849ca6e7f94fbc2b2b8de3da33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00604-017-2246-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00604-017-2246-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Huang, Yan Li</creatorcontrib><creatorcontrib>Mo, Shi</creatorcontrib><creatorcontrib>Gao, Zhong Feng</creatorcontrib><creatorcontrib>Chen, Jing Rong</creatorcontrib><creatorcontrib>Lei, Jing Lei</creatorcontrib><creatorcontrib>Luo, Hong Qun</creatorcontrib><creatorcontrib>Li, Nian Bing</creatorcontrib><title>Amperometric biosensor for microRNA based on the use of tetrahedral DNA nanostructure probes and guanine nanowire amplification</title><title>Mikrochimica acta (1966)</title><addtitle>Microchim Acta</addtitle><description>MicroRNAs are endogenous noncoding RNAs that play critical roles in biological processes and can be considered as molecular markers for early diagnosis and pathogenesis of diseases. The authors describe a highly sensitive electrochemical biosensor for microRNA that is based on the use of tetrahedral DNA nanostructure probes and guanine nanowire amplification. The DNA tetrahedral probe is self-assembled on a gold electrode and enhances reactivity, accessibility, and molecular recognition efficiency. Combined with the tetrahedral probe, the guanine nanowire amplifies the signal and improves the analytical performance of the biosensor. Operated best at a voltage of typically 150 mV (vs. Ag/AgCl), the sensor has a linear response to the logarithmic microRNA concentration in the 500 f. to 10 nM range, with a 176 f. detection limit. It is highly selective and can be applied to real samples. It is concluded that this strategy has a good potential with respect to the determination of microRNA in clinical diagnosis and in biological research.
Graphical abstract
Schematic of a tetrahedral DNA nanostructure-based amperometric biosensor coupled to guanine nanowire amplification for analysis of microRNA-21. This strategy is highly selective and also performs well for the detection of microRNA levels of breast cancer patients.</description><subject>Accessibility</subject><subject>Amplification</subject><subject>Analytical Chemistry</subject><subject>Biological activity</subject><subject>Biosensors</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Deoxyribonucleic acid</subject><subject>Detectors</subject><subject>Diagnosis</subject><subject>DNA</subject><subject>Electric potential</subject><subject>Electrical measurement</subject><subject>Gold</subject><subject>Guanine</subject><subject>Markers</subject><subject>Microengineering</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>Nanochemistry</subject><subject>Nanostructure</subject><subject>Nanotechnology</subject><subject>Nanowires</subject><subject>Original Paper</subject><subject>Pathogenesis</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><issn>0026-3672</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp9kU1rHSEUhqUk0NskPyA7oetJ1VFnXF7SNg2EFkqyFj-ON4Y7eqszlK7y1-PtZNFNg4igz3M8nBehS0quKCHDp0qIJLwjdOgY47Ib36EN5b3sBBn6E7QhhMmulwN7jz7U-kQaKBnfoOftdICSJ5hLdNjGXCHVXHBoe4qu5J_ft9iaCh7nhOdHwEsFnAOem2EewRezx58bk0zKdS6Lm5cC-FCyhYpN8ni3mBQT_AV-x_ZmpsM-hujMHHM6R6fB7CtcvJ5n6OHrl_vrb93dj5vb6-1d5zhXcwdeWdsbboFy4b0QYiQ9t5x4wckYghIKnFUjV85IGILiwTpmmR099N70_Rn6uNZtnf1aoM76KS8ltS81VXIUXDE5vE0xRhWl6ljraqV2Zg86ppDbKFxbHtrIcoIQ2_12oKJlwrhoAl2FNs9aCwR9KHEy5Y-mRB_j02t8uqWij_HpsTlsdWpj0w7KP638V3oBJCWeoA</recordid><startdate>20170801</startdate><enddate>20170801</enddate><creator>Huang, Yan Li</creator><creator>Mo, Shi</creator><creator>Gao, Zhong Feng</creator><creator>Chen, Jing Rong</creator><creator>Lei, Jing Lei</creator><creator>Luo, Hong Qun</creator><creator>Li, Nian Bing</creator><general>Springer Vienna</general><general>Springer</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>HCIFZ</scope><scope>KB.</scope><scope>M0S</scope><scope>M1P</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20170801</creationdate><title>Amperometric biosensor for microRNA based on the use of tetrahedral DNA nanostructure probes and guanine nanowire amplification</title><author>Huang, Yan Li ; 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The authors describe a highly sensitive electrochemical biosensor for microRNA that is based on the use of tetrahedral DNA nanostructure probes and guanine nanowire amplification. The DNA tetrahedral probe is self-assembled on a gold electrode and enhances reactivity, accessibility, and molecular recognition efficiency. Combined with the tetrahedral probe, the guanine nanowire amplifies the signal and improves the analytical performance of the biosensor. Operated best at a voltage of typically 150 mV (vs. Ag/AgCl), the sensor has a linear response to the logarithmic microRNA concentration in the 500 f. to 10 nM range, with a 176 f. detection limit. It is highly selective and can be applied to real samples. It is concluded that this strategy has a good potential with respect to the determination of microRNA in clinical diagnosis and in biological research.
Graphical abstract
Schematic of a tetrahedral DNA nanostructure-based amperometric biosensor coupled to guanine nanowire amplification for analysis of microRNA-21. This strategy is highly selective and also performs well for the detection of microRNA levels of breast cancer patients.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><doi>10.1007/s00604-017-2246-8</doi><tpages>8</tpages></addata></record> |
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| subjects | Accessibility Amplification Analytical Chemistry Biological activity Biosensors Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Deoxyribonucleic acid Detectors Diagnosis DNA Electric potential Electrical measurement Gold Guanine Markers Microengineering MicroRNA MicroRNAs Nanochemistry Nanostructure Nanotechnology Nanowires Original Paper Pathogenesis Ribonucleic acid RNA |
| title | Amperometric biosensor for microRNA based on the use of tetrahedral DNA nanostructure probes and guanine nanowire amplification |
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