A sensitive colorimetric assay for cholesterol based on the peroxidase-like activity of MoS2 nanosheets
The authors describe a colorimetric method for the determination of cholesterol in human serum. Cholesterol is enzymatically oxidized by oxygen in the presence of cholesterol oxidase (ChOx) to produce 4-cholestene-3-one and hydrogen peroxide (H 2 O 2 ). Due to the peroxidase-like activity of MoS 2 n...
Gespeichert in:
| Veröffentlicht in: | Mikrochimica acta (1966) 2017-04, Vol.184 (4), p.1233-1237 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1237 |
|---|---|
| container_issue | 4 |
| container_start_page | 1233 |
| container_title | Mikrochimica acta (1966) |
| container_volume | 184 |
| creator | Lin, Tianran Zhong, Liangshuang Chen, Huan Li, Zhihong Song, Zhiping Guo, Liangqia Fu, Fengfu |
| description | The authors describe a colorimetric method for the determination of cholesterol in human serum. Cholesterol is enzymatically oxidized by oxygen in the presence of cholesterol oxidase (ChOx) to produce 4-cholestene-3-one and hydrogen peroxide (H
2
O
2
). Due to the peroxidase-like activity of MoS
2
nanosheets, H
2
O
2
can oxidize the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) to give a blue product. The increase in the absorbance of the acidic oxTMB solution at 450 nm is used for quantification of cholesterol. Under the optimal conditions, the increase in absorbance is proportional to the concentration of cholesterol in the range from 2 to 200 μmol⋅L
−1
, and the limit of detection is 0.76 μmol⋅L
−1
. The color change from pale yellow to blue color can also be detected visually for cholesterol concentrations as low as 20 μmol⋅L
−1
. Such an enzyme mimetic-based assay possesses advantages over enzyme-based assays in terms of costs, stability against denaturation, and protease digestion. The assay was applied to the determination of free cholesterol and of total cholesterol (after hydrolysis using an esterase) in spiked human serum, and it gave satisfactory recoveries.
Graphical abstract
Schematic presentation of the colorimetric assay of cholesterol. Cholesterol is oxidized by the catalysis of cholesterol oxidase to produce 4-cholestene-3-one and hydrogen peroxide (H
2
O
2
) which oxidizes the substrate 3,3′,5,5′-tetramethylbenzidine (TMB) to produce a blue product (oxTMB) due to the pseudo-enzymatic action of MoS
2
nanosheets. |
| doi_str_mv | 10.1007/s00604-017-2147-x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1968438825</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1968438825</sourcerecordid><originalsourceid>FETCH-LOGICAL-c344t-861fa887b7038afd76a3adb7d9f26bb08c4626ac2a0d382e4caa980afafd09983</originalsourceid><addsrcrecordid>eNp9kE1LAzEQhoMoWKs_wFvAc3Ty0SR7LMUvqHhQzyGbTdqt66YmW2n_vSn14EVPA8PzvjM8CF1SuKYA6iYDSBAEqCKMCkW2R2hEBZdkAoofoxEAk4RLxU7RWc4rKKBkYoQWU5x9n9uh_fLYxS6m9sMPqXXY5mx3OMSE3TJ2Pg8-xQ7XNvsGxx4PS4_XZbVtm7IiXfvusXWlph12OAb8FF8Y7m0f89L7IZ-jk2C77C9-5hi93d2-zh7I_Pn-cTadE8eFGIiWNFitVa2AaxsaJS23Ta2aKjBZ16CdkExaxyw0XDMvnLWVBhsKC1Wl-RhdHXrXKX5uytdmFTepLycNraQWXGs2-ZfSGtSESZCFogfKpZhz8sGsix2bdoaC2Vs3B-umyDR762ZbMuyQyYXtFz79av4z9A3KhYZP</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1968438825</pqid></control><display><type>article</type><title>A sensitive colorimetric assay for cholesterol based on the peroxidase-like activity of MoS2 nanosheets</title><source>SpringerLink Journals - AutoHoldings</source><creator>Lin, Tianran ; Zhong, Liangshuang ; Chen, Huan ; Li, Zhihong ; Song, Zhiping ; Guo, Liangqia ; Fu, Fengfu</creator><creatorcontrib>Lin, Tianran ; Zhong, Liangshuang ; Chen, Huan ; Li, Zhihong ; Song, Zhiping ; Guo, Liangqia ; Fu, Fengfu</creatorcontrib><description>The authors describe a colorimetric method for the determination of cholesterol in human serum. Cholesterol is enzymatically oxidized by oxygen in the presence of cholesterol oxidase (ChOx) to produce 4-cholestene-3-one and hydrogen peroxide (H
2
O
2
). Due to the peroxidase-like activity of MoS
2
nanosheets, H
2
O
2
can oxidize the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) to give a blue product. The increase in the absorbance of the acidic oxTMB solution at 450 nm is used for quantification of cholesterol. Under the optimal conditions, the increase in absorbance is proportional to the concentration of cholesterol in the range from 2 to 200 μmol⋅L
−1
, and the limit of detection is 0.76 μmol⋅L
−1
. The color change from pale yellow to blue color can also be detected visually for cholesterol concentrations as low as 20 μmol⋅L
−1
. Such an enzyme mimetic-based assay possesses advantages over enzyme-based assays in terms of costs, stability against denaturation, and protease digestion. The assay was applied to the determination of free cholesterol and of total cholesterol (after hydrolysis using an esterase) in spiked human serum, and it gave satisfactory recoveries.
Graphical abstract
Schematic presentation of the colorimetric assay of cholesterol. Cholesterol is oxidized by the catalysis of cholesterol oxidase to produce 4-cholestene-3-one and hydrogen peroxide (H
2
O
2
) which oxidizes the substrate 3,3′,5,5′-tetramethylbenzidine (TMB) to produce a blue product (oxTMB) due to the pseudo-enzymatic action of MoS
2
nanosheets.</description><identifier>ISSN: 0026-3672</identifier><identifier>EISSN: 1436-5073</identifier><identifier>DOI: 10.1007/s00604-017-2147-x</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Absorbance ; Analytical Chemistry ; Assaying ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Cholesterol ; Cholesterol oxidase ; Color ; Colorimetry ; Denaturation ; Enzymes ; Hydrogen peroxide ; Microengineering ; Molybdenum disulfide ; Nanochemistry ; Nanosheets ; Nanotechnology ; Peroxidase ; Short Communication ; Substrates</subject><ispartof>Mikrochimica acta (1966), 2017-04, Vol.184 (4), p.1233-1237</ispartof><rights>Springer-Verlag Wien 2017</rights><rights>Copyright Springer Science & Business Media 2017</rights><rights>Microchimica Acta is a copyright of Springer, (2017). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c344t-861fa887b7038afd76a3adb7d9f26bb08c4626ac2a0d382e4caa980afafd09983</citedby><cites>FETCH-LOGICAL-c344t-861fa887b7038afd76a3adb7d9f26bb08c4626ac2a0d382e4caa980afafd09983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00604-017-2147-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00604-017-2147-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids></links><search><creatorcontrib>Lin, Tianran</creatorcontrib><creatorcontrib>Zhong, Liangshuang</creatorcontrib><creatorcontrib>Chen, Huan</creatorcontrib><creatorcontrib>Li, Zhihong</creatorcontrib><creatorcontrib>Song, Zhiping</creatorcontrib><creatorcontrib>Guo, Liangqia</creatorcontrib><creatorcontrib>Fu, Fengfu</creatorcontrib><title>A sensitive colorimetric assay for cholesterol based on the peroxidase-like activity of MoS2 nanosheets</title><title>Mikrochimica acta (1966)</title><addtitle>Microchim Acta</addtitle><description>The authors describe a colorimetric method for the determination of cholesterol in human serum. Cholesterol is enzymatically oxidized by oxygen in the presence of cholesterol oxidase (ChOx) to produce 4-cholestene-3-one and hydrogen peroxide (H
2
O
2
). Due to the peroxidase-like activity of MoS
2
nanosheets, H
2
O
2
can oxidize the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) to give a blue product. The increase in the absorbance of the acidic oxTMB solution at 450 nm is used for quantification of cholesterol. Under the optimal conditions, the increase in absorbance is proportional to the concentration of cholesterol in the range from 2 to 200 μmol⋅L
−1
, and the limit of detection is 0.76 μmol⋅L
−1
. The color change from pale yellow to blue color can also be detected visually for cholesterol concentrations as low as 20 μmol⋅L
−1
. Such an enzyme mimetic-based assay possesses advantages over enzyme-based assays in terms of costs, stability against denaturation, and protease digestion. The assay was applied to the determination of free cholesterol and of total cholesterol (after hydrolysis using an esterase) in spiked human serum, and it gave satisfactory recoveries.
Graphical abstract
Schematic presentation of the colorimetric assay of cholesterol. Cholesterol is oxidized by the catalysis of cholesterol oxidase to produce 4-cholestene-3-one and hydrogen peroxide (H
2
O
2
) which oxidizes the substrate 3,3′,5,5′-tetramethylbenzidine (TMB) to produce a blue product (oxTMB) due to the pseudo-enzymatic action of MoS
2
nanosheets.</description><subject>Absorbance</subject><subject>Analytical Chemistry</subject><subject>Assaying</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Cholesterol</subject><subject>Cholesterol oxidase</subject><subject>Color</subject><subject>Colorimetry</subject><subject>Denaturation</subject><subject>Enzymes</subject><subject>Hydrogen peroxide</subject><subject>Microengineering</subject><subject>Molybdenum disulfide</subject><subject>Nanochemistry</subject><subject>Nanosheets</subject><subject>Nanotechnology</subject><subject>Peroxidase</subject><subject>Short Communication</subject><subject>Substrates</subject><issn>0026-3672</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp9kE1LAzEQhoMoWKs_wFvAc3Ty0SR7LMUvqHhQzyGbTdqt66YmW2n_vSn14EVPA8PzvjM8CF1SuKYA6iYDSBAEqCKMCkW2R2hEBZdkAoofoxEAk4RLxU7RWc4rKKBkYoQWU5x9n9uh_fLYxS6m9sMPqXXY5mx3OMSE3TJ2Pg8-xQ7XNvsGxx4PS4_XZbVtm7IiXfvusXWlph12OAb8FF8Y7m0f89L7IZ-jk2C77C9-5hi93d2-zh7I_Pn-cTadE8eFGIiWNFitVa2AaxsaJS23Ta2aKjBZ16CdkExaxyw0XDMvnLWVBhsKC1Wl-RhdHXrXKX5uytdmFTepLycNraQWXGs2-ZfSGtSESZCFogfKpZhz8sGsix2bdoaC2Vs3B-umyDR762ZbMuyQyYXtFz79av4z9A3KhYZP</recordid><startdate>20170401</startdate><enddate>20170401</enddate><creator>Lin, Tianran</creator><creator>Zhong, Liangshuang</creator><creator>Chen, Huan</creator><creator>Li, Zhihong</creator><creator>Song, Zhiping</creator><creator>Guo, Liangqia</creator><creator>Fu, Fengfu</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>HCIFZ</scope><scope>KB.</scope><scope>M0S</scope><scope>M1P</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20170401</creationdate><title>A sensitive colorimetric assay for cholesterol based on the peroxidase-like activity of MoS2 nanosheets</title><author>Lin, Tianran ; Zhong, Liangshuang ; Chen, Huan ; Li, Zhihong ; Song, Zhiping ; Guo, Liangqia ; Fu, Fengfu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c344t-861fa887b7038afd76a3adb7d9f26bb08c4626ac2a0d382e4caa980afafd09983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Absorbance</topic><topic>Analytical Chemistry</topic><topic>Assaying</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Cholesterol</topic><topic>Cholesterol oxidase</topic><topic>Color</topic><topic>Colorimetry</topic><topic>Denaturation</topic><topic>Enzymes</topic><topic>Hydrogen peroxide</topic><topic>Microengineering</topic><topic>Molybdenum disulfide</topic><topic>Nanochemistry</topic><topic>Nanosheets</topic><topic>Nanotechnology</topic><topic>Peroxidase</topic><topic>Short Communication</topic><topic>Substrates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, Tianran</creatorcontrib><creatorcontrib>Zhong, Liangshuang</creatorcontrib><creatorcontrib>Chen, Huan</creatorcontrib><creatorcontrib>Li, Zhihong</creatorcontrib><creatorcontrib>Song, Zhiping</creatorcontrib><creatorcontrib>Guo, Liangqia</creatorcontrib><creatorcontrib>Fu, Fengfu</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>SciTech Premium Collection</collection><collection>Materials Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, Tianran</au><au>Zhong, Liangshuang</au><au>Chen, Huan</au><au>Li, Zhihong</au><au>Song, Zhiping</au><au>Guo, Liangqia</au><au>Fu, Fengfu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A sensitive colorimetric assay for cholesterol based on the peroxidase-like activity of MoS2 nanosheets</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><date>2017-04-01</date><risdate>2017</risdate><volume>184</volume><issue>4</issue><spage>1233</spage><epage>1237</epage><pages>1233-1237</pages><issn>0026-3672</issn><eissn>1436-5073</eissn><abstract>The authors describe a colorimetric method for the determination of cholesterol in human serum. Cholesterol is enzymatically oxidized by oxygen in the presence of cholesterol oxidase (ChOx) to produce 4-cholestene-3-one and hydrogen peroxide (H
2
O
2
). Due to the peroxidase-like activity of MoS
2
nanosheets, H
2
O
2
can oxidize the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) to give a blue product. The increase in the absorbance of the acidic oxTMB solution at 450 nm is used for quantification of cholesterol. Under the optimal conditions, the increase in absorbance is proportional to the concentration of cholesterol in the range from 2 to 200 μmol⋅L
−1
, and the limit of detection is 0.76 μmol⋅L
−1
. The color change from pale yellow to blue color can also be detected visually for cholesterol concentrations as low as 20 μmol⋅L
−1
. Such an enzyme mimetic-based assay possesses advantages over enzyme-based assays in terms of costs, stability against denaturation, and protease digestion. The assay was applied to the determination of free cholesterol and of total cholesterol (after hydrolysis using an esterase) in spiked human serum, and it gave satisfactory recoveries.
Graphical abstract
Schematic presentation of the colorimetric assay of cholesterol. Cholesterol is oxidized by the catalysis of cholesterol oxidase to produce 4-cholestene-3-one and hydrogen peroxide (H
2
O
2
) which oxidizes the substrate 3,3′,5,5′-tetramethylbenzidine (TMB) to produce a blue product (oxTMB) due to the pseudo-enzymatic action of MoS
2
nanosheets.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><doi>10.1007/s00604-017-2147-x</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0026-3672 |
| ispartof | Mikrochimica acta (1966), 2017-04, Vol.184 (4), p.1233-1237 |
| issn | 0026-3672 1436-5073 |
| language | eng |
| recordid | cdi_proquest_journals_1968438825 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Absorbance Analytical Chemistry Assaying Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Cholesterol Cholesterol oxidase Color Colorimetry Denaturation Enzymes Hydrogen peroxide Microengineering Molybdenum disulfide Nanochemistry Nanosheets Nanotechnology Peroxidase Short Communication Substrates |
| title | A sensitive colorimetric assay for cholesterol based on the peroxidase-like activity of MoS2 nanosheets |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T20%3A26%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20sensitive%20colorimetric%20assay%20for%20cholesterol%20based%20on%20the%20peroxidase-like%20activity%20of%20MoS2%20nanosheets&rft.jtitle=Mikrochimica%20acta%20(1966)&rft.au=Lin,%20Tianran&rft.date=2017-04-01&rft.volume=184&rft.issue=4&rft.spage=1233&rft.epage=1237&rft.pages=1233-1237&rft.issn=0026-3672&rft.eissn=1436-5073&rft_id=info:doi/10.1007/s00604-017-2147-x&rft_dat=%3Cproquest_cross%3E1968438825%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1968438825&rft_id=info:pmid/&rfr_iscdi=true |