Expression of the zebrafish β-defensin 3 mature peptide in Pichia pastoris and its purification and antibacterial activity
Defensins are abundant in cells and tissues that are involved in host defense against microbial infections. zfDB3 (zebrafish β-defensin 3) is one of 3 copies of defensin β-like genes from zebrafish ( Danio rerio ). Here we focus on mzfDB3 , which is the gene encoding for the zebrafish β-defensin 3 m...
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| Veröffentlicht in: | Applied biochemistry and microbiology 2017-11, Vol.53 (6), p.661-668 |
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| creator | Tao, Y. Song, C. F. Li, W. |
| description | Defensins are abundant in cells and tissues that are involved in host defense against microbial infections.
zfDB3
(zebrafish β-defensin 3) is one of 3 copies of defensin β-like genes from zebrafish (
Danio rerio
). Here we focus on
mzfDB3
, which is the gene encoding for the zebrafish β-defensin 3 mature peptide. A codon-optimized
mzfDB3
gene with a 6×His-tag at the 3′-end was inserted into the pPICZαA expression vector and transformed into
Pichia pastoris
X-33 cells. The recombinant zebrafish β-defensin 3 mature peptide (
rmzfDB3
) was induced with 1.0% methanol at 29°C for 72 h and purified by immobilized metal affinity chromatography. MALDI-TOF/TOF analysis confirmed the expected purified product (
rmzfDB3
, 5.9 kDa). Fermentation supernatant, which contained rmzfDB3, showed antibacterial activity against Grampositive (i.e.,
Listeria monocytogenes, Staphylococcus aureus
, and
Bacillus cereus
) and Gram-negative (i.e.,
Escherchia coli
BL21,
Vibrio parahaemolyticus
,
Salmonella lignieres
, and
Pseudomonas aeruginosa
) bacteria. |
| doi_str_mv | 10.1134/S0003683817060126 |
| format | Article |
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zfDB3
(zebrafish β-defensin 3) is one of 3 copies of defensin β-like genes from zebrafish (
Danio rerio
). Here we focus on
mzfDB3
, which is the gene encoding for the zebrafish β-defensin 3 mature peptide. A codon-optimized
mzfDB3
gene with a 6×His-tag at the 3′-end was inserted into the pPICZαA expression vector and transformed into
Pichia pastoris
X-33 cells. The recombinant zebrafish β-defensin 3 mature peptide (
rmzfDB3
) was induced with 1.0% methanol at 29°C for 72 h and purified by immobilized metal affinity chromatography. MALDI-TOF/TOF analysis confirmed the expected purified product (
rmzfDB3
, 5.9 kDa). Fermentation supernatant, which contained rmzfDB3, showed antibacterial activity against Grampositive (i.e.,
Listeria monocytogenes, Staphylococcus aureus
, and
Bacillus cereus
) and Gram-negative (i.e.,
Escherchia coli
BL21,
Vibrio parahaemolyticus
,
Salmonella lignieres
, and
Pseudomonas aeruginosa
) bacteria.</description><identifier>ISSN: 0003-6838</identifier><identifier>EISSN: 1608-3024</identifier><identifier>DOI: 10.1134/S0003683817060126</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Affinity chromatography ; Antibacterial activity ; Bacteria ; Biochemistry ; Biomedical and Life Sciences ; Danio rerio ; Defensins ; Fermentation ; Life Sciences ; Listeria ; Listeria monocytogenes ; Medical Microbiology ; Microbiology ; Microorganisms ; Peptides ; Pichia pastoris ; Pseudomonas aeruginosa ; Purification ; Salmonella ; Waterborne diseases ; Yeast ; Zebrafish</subject><ispartof>Applied biochemistry and microbiology, 2017-11, Vol.53 (6), p.661-668</ispartof><rights>Pleiades Publishing, Inc. 2017</rights><rights>Copyright Springer Science & Business Media Nov 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-92336198ff61d06335fec7fba877f7d7fb8aa2f9faa5c1577b1e9161a1b529f23</citedby><cites>FETCH-LOGICAL-c316t-92336198ff61d06335fec7fba877f7d7fb8aa2f9faa5c1577b1e9161a1b529f23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0003683817060126$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0003683817060126$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,41486,42555,51317</link.rule.ids></links><search><creatorcontrib>Tao, Y.</creatorcontrib><creatorcontrib>Song, C. F.</creatorcontrib><creatorcontrib>Li, W.</creatorcontrib><title>Expression of the zebrafish β-defensin 3 mature peptide in Pichia pastoris and its purification and antibacterial activity</title><title>Applied biochemistry and microbiology</title><addtitle>Appl Biochem Microbiol</addtitle><description>Defensins are abundant in cells and tissues that are involved in host defense against microbial infections.
zfDB3
(zebrafish β-defensin 3) is one of 3 copies of defensin β-like genes from zebrafish (
Danio rerio
). Here we focus on
mzfDB3
, which is the gene encoding for the zebrafish β-defensin 3 mature peptide. A codon-optimized
mzfDB3
gene with a 6×His-tag at the 3′-end was inserted into the pPICZαA expression vector and transformed into
Pichia pastoris
X-33 cells. The recombinant zebrafish β-defensin 3 mature peptide (
rmzfDB3
) was induced with 1.0% methanol at 29°C for 72 h and purified by immobilized metal affinity chromatography. MALDI-TOF/TOF analysis confirmed the expected purified product (
rmzfDB3
, 5.9 kDa). Fermentation supernatant, which contained rmzfDB3, showed antibacterial activity against Grampositive (i.e.,
Listeria monocytogenes, Staphylococcus aureus
, and
Bacillus cereus
) and Gram-negative (i.e.,
Escherchia coli
BL21,
Vibrio parahaemolyticus
,
Salmonella lignieres
, and
Pseudomonas aeruginosa
) bacteria.</description><subject>Affinity chromatography</subject><subject>Antibacterial activity</subject><subject>Bacteria</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Danio rerio</subject><subject>Defensins</subject><subject>Fermentation</subject><subject>Life Sciences</subject><subject>Listeria</subject><subject>Listeria monocytogenes</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Peptides</subject><subject>Pichia pastoris</subject><subject>Pseudomonas aeruginosa</subject><subject>Purification</subject><subject>Salmonella</subject><subject>Waterborne diseases</subject><subject>Yeast</subject><subject>Zebrafish</subject><issn>0003-6838</issn><issn>1608-3024</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1UM1KAzEQDqJgrT6At4Dn1UzSTTZHKfUHCgrqeZndTWxKu7smqVh9Kx_EZzJLPQjiab6Z72fgI-QU2DmAmFw8MMaELEQBikkGXO6REUhWZILxyT4ZDXQ28IfkKIRlWrUs9Ih8zN56b0JwXUs7S-PC0HdTebQuLOjXZ9YYa9rgWiroGuPGG9qbPrrG0HS7d_XCIe0xxM67QLFtqIuB9hvvrKsxDqnDEdvoKqyj8Q5XNAH36uL2mBxYXAVz8jPH5Olq9ji9yeZ317fTy3lWC5Ax01wICbqwVkLDpBC5NbWyFRZKWdUkVCByqy1iXkOuVAVGgwSEKufacjEmZ7vc3ncvGxNiuew2vk0vS9BSCQ6S50kFO1XtuxC8sWXv3Rr9tgRWDh2XfzpOHr7zhKRtn43_lfyv6RvMh4A0</recordid><startdate>20171101</startdate><enddate>20171101</enddate><creator>Tao, Y.</creator><creator>Song, C. F.</creator><creator>Li, W.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope></search><sort><creationdate>20171101</creationdate><title>Expression of the zebrafish β-defensin 3 mature peptide in Pichia pastoris and its purification and antibacterial activity</title><author>Tao, Y. ; Song, C. F. ; Li, W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-92336198ff61d06335fec7fba877f7d7fb8aa2f9faa5c1577b1e9161a1b529f23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Affinity chromatography</topic><topic>Antibacterial activity</topic><topic>Bacteria</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Danio rerio</topic><topic>Defensins</topic><topic>Fermentation</topic><topic>Life Sciences</topic><topic>Listeria</topic><topic>Listeria monocytogenes</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Microorganisms</topic><topic>Peptides</topic><topic>Pichia pastoris</topic><topic>Pseudomonas aeruginosa</topic><topic>Purification</topic><topic>Salmonella</topic><topic>Waterborne diseases</topic><topic>Yeast</topic><topic>Zebrafish</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tao, Y.</creatorcontrib><creatorcontrib>Song, C. F.</creatorcontrib><creatorcontrib>Li, W.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><jtitle>Applied biochemistry and microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tao, Y.</au><au>Song, C. F.</au><au>Li, W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of the zebrafish β-defensin 3 mature peptide in Pichia pastoris and its purification and antibacterial activity</atitle><jtitle>Applied biochemistry and microbiology</jtitle><stitle>Appl Biochem Microbiol</stitle><date>2017-11-01</date><risdate>2017</risdate><volume>53</volume><issue>6</issue><spage>661</spage><epage>668</epage><pages>661-668</pages><issn>0003-6838</issn><eissn>1608-3024</eissn><abstract>Defensins are abundant in cells and tissues that are involved in host defense against microbial infections.
zfDB3
(zebrafish β-defensin 3) is one of 3 copies of defensin β-like genes from zebrafish (
Danio rerio
). Here we focus on
mzfDB3
, which is the gene encoding for the zebrafish β-defensin 3 mature peptide. A codon-optimized
mzfDB3
gene with a 6×His-tag at the 3′-end was inserted into the pPICZαA expression vector and transformed into
Pichia pastoris
X-33 cells. The recombinant zebrafish β-defensin 3 mature peptide (
rmzfDB3
) was induced with 1.0% methanol at 29°C for 72 h and purified by immobilized metal affinity chromatography. MALDI-TOF/TOF analysis confirmed the expected purified product (
rmzfDB3
, 5.9 kDa). Fermentation supernatant, which contained rmzfDB3, showed antibacterial activity against Grampositive (i.e.,
Listeria monocytogenes, Staphylococcus aureus
, and
Bacillus cereus
) and Gram-negative (i.e.,
Escherchia coli
BL21,
Vibrio parahaemolyticus
,
Salmonella lignieres
, and
Pseudomonas aeruginosa
) bacteria.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S0003683817060126</doi><tpages>8</tpages></addata></record> |
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| ispartof | Applied biochemistry and microbiology, 2017-11, Vol.53 (6), p.661-668 |
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| language | eng |
| recordid | cdi_proquest_journals_1967321625 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Affinity chromatography Antibacterial activity Bacteria Biochemistry Biomedical and Life Sciences Danio rerio Defensins Fermentation Life Sciences Listeria Listeria monocytogenes Medical Microbiology Microbiology Microorganisms Peptides Pichia pastoris Pseudomonas aeruginosa Purification Salmonella Waterborne diseases Yeast Zebrafish |
| title | Expression of the zebrafish β-defensin 3 mature peptide in Pichia pastoris and its purification and antibacterial activity |
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