Facile-one pot-green synthesis, antibacterial, antifungal, antioxidant and antiplatelet activities of lignin capped silver nanoparticles: A promising therapeutic agent
The current work portrays the green synthesis of Lignin Capped Silver Nanoparticles (LCSN) and their antibacterial, antifungal, antioxidant and antiplatelet potential. The LCSN was synthesized in water using a carbohydrate based polymer ‘lignin’ as the reducing and capping agents. The peak at 406nm...
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description | The current work portrays the green synthesis of Lignin Capped Silver Nanoparticles (LCSN) and their antibacterial, antifungal, antioxidant and antiplatelet potential. The LCSN was synthesized in water using a carbohydrate based polymer ‘lignin’ as the reducing and capping agents. The peak at 406nm (λmax) in the UV–Vis., spectrum and EDX analysis confirmed 1.68% (w/w) of silver was found to be loaded on lignin. The characteristic sharp peaks appeared in the PXRD spectrum showed fcc crystalline structure LCSN. SEM and TEM images indicated that the spherical Ag-NPs were well dispersed on lignin with an average particle size of ~10–15nm. LCSN showed antibacterial and antifungal activity against human pathogens S. aureus, E. coli and A. niger and the percentage of zone of inhibition was found to be 10%, 12% and 80% respectively. Further, LCSN was evaluated for antioxidant potential using DPPH scavenging assay, interestingly it showed antioxidant activity and the percentage against positive control vitamin C was found to be 70%. Furthermore, LCSN did not interfere in plasma coagulation; however, it found to inhibit agonist ADP induced platelet aggregation of human platelet rich plasma. The observed inhibition was found to be 37% and the calculated IC50 value was found to be 9mg/mL. LCSN did not lyses RBC membrane when assayed hemolytic activity suggesting its non-toxic nature.
[Display omitted]
•Silver nanospheres with an average size of 10-15nm were synthesized first time in water and stabilized by lignin.•Lignin is a cheap, naturally abundant, non-toxic, biodegradable, environmentally benign and flexible biopolymer.•Lignin Capped Silver Nanoparticles (LCSN) was well characterized by UV-Vis., FT-IR, SEM, TEM, EDX, and PXRD.•LCSN found a potential antibacterial, antifungal, antioxidant and antiplatelet activity. |
doi_str_mv | 10.1016/j.msec.2017.07.054 |
format | Article |
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[Display omitted]
•Silver nanospheres with an average size of 10-15nm were synthesized first time in water and stabilized by lignin.•Lignin is a cheap, naturally abundant, non-toxic, biodegradable, environmentally benign and flexible biopolymer.•Lignin Capped Silver Nanoparticles (LCSN) was well characterized by UV-Vis., FT-IR, SEM, TEM, EDX, and PXRD.•LCSN found a potential antibacterial, antifungal, antioxidant and antiplatelet activity.</description><identifier>ISSN: 0928-4931</identifier><identifier>EISSN: 1873-0191</identifier><identifier>DOI: 10.1016/j.msec.2017.07.054</identifier><identifier>PMID: 28887963</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adenosine diphosphate ; Anti-Bacterial Agents ; Antifungal activity ; Antifungal Agents ; Antimicrobial activity ; Antioxidant activity ; Antioxidants ; Antiplatelet ; Ascorbic acid ; Biodegradable ; Capping ; Carbohydrates ; Chemical compounds ; Chemical synthesis ; Coagulation ; E coli ; Environmentally benign ; Escherichia coli ; Fungicides ; Inhibition ; Lignin ; Materials science ; Metal Nanoparticles ; Nanoparticles ; Pathogens ; Pharmacology ; Plasma recalcification ; Platelet aggregation ; Platelet Aggregation Inhibitors ; Silver ; Silver nanoparticles ; Staphylococcus aureus ; Vitamin C</subject><ispartof>Materials Science & Engineering C, 2017-12, Vol.81, p.182-190</ispartof><rights>2017 Elsevier B.V.</rights><rights>Copyright © 2017 Elsevier B.V. All rights reserved.</rights><rights>Copyright Elsevier BV Dec 1, 2017</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-c7e385399c45a7a7fde3faad159368bd719c1bfb35d92ecc0d45171984150fae3</citedby><cites>FETCH-LOGICAL-c423t-c7e385399c45a7a7fde3faad159368bd719c1bfb35d92ecc0d45171984150fae3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.msec.2017.07.054$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28887963$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Marulasiddeshwara, M.B.</creatorcontrib><creatorcontrib>Dakshayani, S.S.</creatorcontrib><creatorcontrib>Sharath Kumar, M.N.</creatorcontrib><creatorcontrib>Chethana, R.</creatorcontrib><creatorcontrib>Raghavendra Kumar, P.</creatorcontrib><creatorcontrib>Devaraja, S.</creatorcontrib><title>Facile-one pot-green synthesis, antibacterial, antifungal, antioxidant and antiplatelet activities of lignin capped silver nanoparticles: A promising therapeutic agent</title><title>Materials Science & Engineering C</title><addtitle>Mater Sci Eng C Mater Biol Appl</addtitle><description>The current work portrays the green synthesis of Lignin Capped Silver Nanoparticles (LCSN) and their antibacterial, antifungal, antioxidant and antiplatelet potential. The LCSN was synthesized in water using a carbohydrate based polymer ‘lignin’ as the reducing and capping agents. The peak at 406nm (λmax) in the UV–Vis., spectrum and EDX analysis confirmed 1.68% (w/w) of silver was found to be loaded on lignin. The characteristic sharp peaks appeared in the PXRD spectrum showed fcc crystalline structure LCSN. SEM and TEM images indicated that the spherical Ag-NPs were well dispersed on lignin with an average particle size of ~10–15nm. LCSN showed antibacterial and antifungal activity against human pathogens S. aureus, E. coli and A. niger and the percentage of zone of inhibition was found to be 10%, 12% and 80% respectively. Further, LCSN was evaluated for antioxidant potential using DPPH scavenging assay, interestingly it showed antioxidant activity and the percentage against positive control vitamin C was found to be 70%. Furthermore, LCSN did not interfere in plasma coagulation; however, it found to inhibit agonist ADP induced platelet aggregation of human platelet rich plasma. The observed inhibition was found to be 37% and the calculated IC50 value was found to be 9mg/mL. LCSN did not lyses RBC membrane when assayed hemolytic activity suggesting its non-toxic nature.
[Display omitted]
•Silver nanospheres with an average size of 10-15nm were synthesized first time in water and stabilized by lignin.•Lignin is a cheap, naturally abundant, non-toxic, biodegradable, environmentally benign and flexible biopolymer.•Lignin Capped Silver Nanoparticles (LCSN) was well characterized by UV-Vis., FT-IR, SEM, TEM, EDX, and PXRD.•LCSN found a potential antibacterial, antifungal, antioxidant and antiplatelet activity.</description><subject>Adenosine diphosphate</subject><subject>Anti-Bacterial Agents</subject><subject>Antifungal activity</subject><subject>Antifungal Agents</subject><subject>Antimicrobial activity</subject><subject>Antioxidant activity</subject><subject>Antioxidants</subject><subject>Antiplatelet</subject><subject>Ascorbic acid</subject><subject>Biodegradable</subject><subject>Capping</subject><subject>Carbohydrates</subject><subject>Chemical compounds</subject><subject>Chemical synthesis</subject><subject>Coagulation</subject><subject>E coli</subject><subject>Environmentally benign</subject><subject>Escherichia coli</subject><subject>Fungicides</subject><subject>Inhibition</subject><subject>Lignin</subject><subject>Materials science</subject><subject>Metal Nanoparticles</subject><subject>Nanoparticles</subject><subject>Pathogens</subject><subject>Pharmacology</subject><subject>Plasma recalcification</subject><subject>Platelet aggregation</subject><subject>Platelet Aggregation Inhibitors</subject><subject>Silver</subject><subject>Silver nanoparticles</subject><subject>Staphylococcus aureus</subject><subject>Vitamin C</subject><issn>0928-4931</issn><issn>1873-0191</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UcFu1DAQtRAVXQo_wAFZ4koWO07iGHGpKgpIlXppz5ZjT8KssnawnVX7RfwmXrbliDTSmxm_eTPWI-QdZ1vOePdpt90nsNuacbllJdrmBdnwXoqKccVfkg1TdV81SvBz8jqlHWNdL2T9ipzXfd9L1YkN-X1tLM5QBQ90CbmaIoCn6dHnn5AwfaTGZxyMzRDRzKdyXP30nIcHdAVL7v7Wy2wyzFAaNuMBM0KiYaQzTh49tWZZwNGE8wEi9caHxcSMdob0mV7SJYY9JvQTLdujWWAtb9RM4PMbcjaaOcHbJ7wg99df766-Vze3335cXd5UtqlFrqwE0bdCKdu0Rho5OhCjMY63SnT94CRXlg_jIFqnarCWuablpdk3vGWjAXFBPpx0yy2_VkhZ78IafVmpueo6JrsChVWfWDaGlCKMeom4N_FRc6aP3uidPnqjj95oVqJtytD7J-l12IP7N_JsRiF8ORGgfPCAEHWyCN6Cwwg2axfwf_p_AIC1pNc</recordid><startdate>20171201</startdate><enddate>20171201</enddate><creator>Marulasiddeshwara, M.B.</creator><creator>Dakshayani, S.S.</creator><creator>Sharath Kumar, M.N.</creator><creator>Chethana, R.</creator><creator>Raghavendra Kumar, P.</creator><creator>Devaraja, S.</creator><general>Elsevier B.V</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope></search><sort><creationdate>20171201</creationdate><title>Facile-one pot-green synthesis, antibacterial, antifungal, antioxidant and antiplatelet activities of lignin capped silver nanoparticles: A promising therapeutic agent</title><author>Marulasiddeshwara, M.B. ; Dakshayani, S.S. ; Sharath Kumar, M.N. ; Chethana, R. ; Raghavendra Kumar, P. ; Devaraja, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-c7e385399c45a7a7fde3faad159368bd719c1bfb35d92ecc0d45171984150fae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adenosine diphosphate</topic><topic>Anti-Bacterial Agents</topic><topic>Antifungal activity</topic><topic>Antifungal Agents</topic><topic>Antimicrobial activity</topic><topic>Antioxidant activity</topic><topic>Antioxidants</topic><topic>Antiplatelet</topic><topic>Ascorbic acid</topic><topic>Biodegradable</topic><topic>Capping</topic><topic>Carbohydrates</topic><topic>Chemical compounds</topic><topic>Chemical synthesis</topic><topic>Coagulation</topic><topic>E coli</topic><topic>Environmentally benign</topic><topic>Escherichia coli</topic><topic>Fungicides</topic><topic>Inhibition</topic><topic>Lignin</topic><topic>Materials science</topic><topic>Metal Nanoparticles</topic><topic>Nanoparticles</topic><topic>Pathogens</topic><topic>Pharmacology</topic><topic>Plasma recalcification</topic><topic>Platelet aggregation</topic><topic>Platelet Aggregation Inhibitors</topic><topic>Silver</topic><topic>Silver nanoparticles</topic><topic>Staphylococcus aureus</topic><topic>Vitamin C</topic><toplevel>online_resources</toplevel><creatorcontrib>Marulasiddeshwara, M.B.</creatorcontrib><creatorcontrib>Dakshayani, S.S.</creatorcontrib><creatorcontrib>Sharath Kumar, M.N.</creatorcontrib><creatorcontrib>Chethana, R.</creatorcontrib><creatorcontrib>Raghavendra Kumar, P.</creatorcontrib><creatorcontrib>Devaraja, S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Materials Science & Engineering C</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marulasiddeshwara, M.B.</au><au>Dakshayani, S.S.</au><au>Sharath Kumar, M.N.</au><au>Chethana, R.</au><au>Raghavendra Kumar, P.</au><au>Devaraja, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Facile-one pot-green synthesis, antibacterial, antifungal, antioxidant and antiplatelet activities of lignin capped silver nanoparticles: A promising therapeutic agent</atitle><jtitle>Materials Science & Engineering C</jtitle><addtitle>Mater Sci Eng C Mater Biol Appl</addtitle><date>2017-12-01</date><risdate>2017</risdate><volume>81</volume><spage>182</spage><epage>190</epage><pages>182-190</pages><issn>0928-4931</issn><eissn>1873-0191</eissn><abstract>The current work portrays the green synthesis of Lignin Capped Silver Nanoparticles (LCSN) and their antibacterial, antifungal, antioxidant and antiplatelet potential. The LCSN was synthesized in water using a carbohydrate based polymer ‘lignin’ as the reducing and capping agents. The peak at 406nm (λmax) in the UV–Vis., spectrum and EDX analysis confirmed 1.68% (w/w) of silver was found to be loaded on lignin. The characteristic sharp peaks appeared in the PXRD spectrum showed fcc crystalline structure LCSN. SEM and TEM images indicated that the spherical Ag-NPs were well dispersed on lignin with an average particle size of ~10–15nm. LCSN showed antibacterial and antifungal activity against human pathogens S. aureus, E. coli and A. niger and the percentage of zone of inhibition was found to be 10%, 12% and 80% respectively. Further, LCSN was evaluated for antioxidant potential using DPPH scavenging assay, interestingly it showed antioxidant activity and the percentage against positive control vitamin C was found to be 70%. Furthermore, LCSN did not interfere in plasma coagulation; however, it found to inhibit agonist ADP induced platelet aggregation of human platelet rich plasma. The observed inhibition was found to be 37% and the calculated IC50 value was found to be 9mg/mL. LCSN did not lyses RBC membrane when assayed hemolytic activity suggesting its non-toxic nature.
[Display omitted]
•Silver nanospheres with an average size of 10-15nm were synthesized first time in water and stabilized by lignin.•Lignin is a cheap, naturally abundant, non-toxic, biodegradable, environmentally benign and flexible biopolymer.•Lignin Capped Silver Nanoparticles (LCSN) was well characterized by UV-Vis., FT-IR, SEM, TEM, EDX, and PXRD.•LCSN found a potential antibacterial, antifungal, antioxidant and antiplatelet activity.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>28887963</pmid><doi>10.1016/j.msec.2017.07.054</doi><tpages>9</tpages></addata></record> |
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subjects | Adenosine diphosphate Anti-Bacterial Agents Antifungal activity Antifungal Agents Antimicrobial activity Antioxidant activity Antioxidants Antiplatelet Ascorbic acid Biodegradable Capping Carbohydrates Chemical compounds Chemical synthesis Coagulation E coli Environmentally benign Escherichia coli Fungicides Inhibition Lignin Materials science Metal Nanoparticles Nanoparticles Pathogens Pharmacology Plasma recalcification Platelet aggregation Platelet Aggregation Inhibitors Silver Silver nanoparticles Staphylococcus aureus Vitamin C |
title | Facile-one pot-green synthesis, antibacterial, antifungal, antioxidant and antiplatelet activities of lignin capped silver nanoparticles: A promising therapeutic agent |
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