Screening of mushrooms bioactivity: piceatannol was identified as a bioactive ingredient in the order Cantharellales
Wild edible mushroom species are appreciated for consumption due to their high nutritional value. The aim of the present study was to examine in vitro beneficial bioactivity of mushroom extracts and to investigate the molecular identity of the active ingredients. In this regard, methanol extracts of...
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| Veröffentlicht in: | European food research & technology 2018-05, Vol.244 (5), p.861-871 |
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| creator | Vasdekis, Efstathios P. Karkabounas, Athanassios Giannakopoulos, Ioannis Savvas, Dimitrios Lekka, Marilena E. |
| description | Wild edible mushroom species are appreciated for consumption due to their high nutritional value. The aim of the present study was to examine in vitro beneficial bioactivity of mushroom extracts and to investigate the molecular identity of the active ingredients. In this regard, methanol extracts of 29 different wild edible mushroom species, that are traditionally consumed by residents in the National Park of North Pindos in North-Western Greece, were examined for antioxidant, antiproliferative, cytotoxic, and pro-apoptotic activities towards a human lung adenocarcinoma cell line A549 by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and by flow cytometry. Certain mushroom species exhibited high antioxidant activity, which was related to their high content in total phenols and flavonoids. Methanol extracts of
Cantharellus cibarius, Cantharellus cinereus, Craterellus cornucopioides
and
Hydnum repandum
, which belong to the order Cantharellales, exhibited high cytotoxicity and induced apoptosis–necrosis to A549 cells. High Performance Liquid Chromatography (HPLC) coupled with Mass Spectrometry analysis revealed as an active ingredient piceatannol ((E)-4-[2-(3,5-dihydroxyphenyl)ethenyl]1,2-benzenediol-3,3′,4,5′-tetrahydroxy-
trans
-stilbene). Piceatannol, according to our best knowledge, is identified for the first time in wild edible mushrooms. Experiments with authentic piceatannol confirmed the potent antiproliferative activity of this compound. Tested mushrooms are promising sources of bioactive compounds. |
| doi_str_mv | 10.1007/s00217-017-3007-y |
| format | Article |
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Cantharellus cibarius, Cantharellus cinereus, Craterellus cornucopioides
and
Hydnum repandum
, which belong to the order Cantharellales, exhibited high cytotoxicity and induced apoptosis–necrosis to A549 cells. High Performance Liquid Chromatography (HPLC) coupled with Mass Spectrometry analysis revealed as an active ingredient piceatannol ((E)-4-[2-(3,5-dihydroxyphenyl)ethenyl]1,2-benzenediol-3,3′,4,5′-tetrahydroxy-
trans
-stilbene). Piceatannol, according to our best knowledge, is identified for the first time in wild edible mushrooms. Experiments with authentic piceatannol confirmed the potent antiproliferative activity of this compound. Tested mushrooms are promising sources of bioactive compounds.</description><identifier>ISSN: 1438-2377</identifier><identifier>EISSN: 1438-2385</identifier><identifier>DOI: 10.1007/s00217-017-3007-y</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Adenocarcinoma ; Agriculture ; Analytical Chemistry ; Antioxidants ; Apoptosis ; Bioactive compounds ; Biocompatibility ; Biological activity ; Biotechnology ; Cantharellales ; Cantharellus ; Chemistry ; Chemistry and Materials Science ; Cytometry ; Cytotoxicity ; Flavonoids ; Flow cytometry ; Food Science ; Forestry ; High performance liquid chromatography ; Liquid chromatography ; Lungs ; Mass spectrometry ; Mass spectroscopy ; Methanol ; Mushrooms ; National parks ; Nutritive value ; Original Paper ; Phenols ; Piceatannol ; Species ; Stilbene ; Toxicity ; trans-Stilbene</subject><ispartof>European food research & technology, 2018-05, Vol.244 (5), p.861-871</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2017</rights><rights>European Food Research and Technology is a copyright of Springer, (2017). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-cb2b4faffd3d60befd79c7c02e16a4d8bd37b9f4cef6523c3266cfdc1fb8e5f73</citedby><cites>FETCH-LOGICAL-c316t-cb2b4faffd3d60befd79c7c02e16a4d8bd37b9f4cef6523c3266cfdc1fb8e5f73</cites><orcidid>0000-0001-8667-4927</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00217-017-3007-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00217-017-3007-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Vasdekis, Efstathios P.</creatorcontrib><creatorcontrib>Karkabounas, Athanassios</creatorcontrib><creatorcontrib>Giannakopoulos, Ioannis</creatorcontrib><creatorcontrib>Savvas, Dimitrios</creatorcontrib><creatorcontrib>Lekka, Marilena E.</creatorcontrib><title>Screening of mushrooms bioactivity: piceatannol was identified as a bioactive ingredient in the order Cantharellales</title><title>European food research & technology</title><addtitle>Eur Food Res Technol</addtitle><description>Wild edible mushroom species are appreciated for consumption due to their high nutritional value. The aim of the present study was to examine in vitro beneficial bioactivity of mushroom extracts and to investigate the molecular identity of the active ingredients. In this regard, methanol extracts of 29 different wild edible mushroom species, that are traditionally consumed by residents in the National Park of North Pindos in North-Western Greece, were examined for antioxidant, antiproliferative, cytotoxic, and pro-apoptotic activities towards a human lung adenocarcinoma cell line A549 by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and by flow cytometry. Certain mushroom species exhibited high antioxidant activity, which was related to their high content in total phenols and flavonoids. Methanol extracts of
Cantharellus cibarius, Cantharellus cinereus, Craterellus cornucopioides
and
Hydnum repandum
, which belong to the order Cantharellales, exhibited high cytotoxicity and induced apoptosis–necrosis to A549 cells. High Performance Liquid Chromatography (HPLC) coupled with Mass Spectrometry analysis revealed as an active ingredient piceatannol ((E)-4-[2-(3,5-dihydroxyphenyl)ethenyl]1,2-benzenediol-3,3′,4,5′-tetrahydroxy-
trans
-stilbene). Piceatannol, according to our best knowledge, is identified for the first time in wild edible mushrooms. Experiments with authentic piceatannol confirmed the potent antiproliferative activity of this compound. Tested mushrooms are promising sources of bioactive compounds.</description><subject>Adenocarcinoma</subject><subject>Agriculture</subject><subject>Analytical Chemistry</subject><subject>Antioxidants</subject><subject>Apoptosis</subject><subject>Bioactive compounds</subject><subject>Biocompatibility</subject><subject>Biological activity</subject><subject>Biotechnology</subject><subject>Cantharellales</subject><subject>Cantharellus</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Cytometry</subject><subject>Cytotoxicity</subject><subject>Flavonoids</subject><subject>Flow cytometry</subject><subject>Food Science</subject><subject>Forestry</subject><subject>High performance liquid chromatography</subject><subject>Liquid chromatography</subject><subject>Lungs</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Methanol</subject><subject>Mushrooms</subject><subject>National parks</subject><subject>Nutritive value</subject><subject>Original Paper</subject><subject>Phenols</subject><subject>Piceatannol</subject><subject>Species</subject><subject>Stilbene</subject><subject>Toxicity</subject><subject>trans-Stilbene</subject><issn>1438-2377</issn><issn>1438-2385</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp1UE1LxDAUDKLguvoDvAU8V_PRpq03WfyCBQ_qOaTJy26WbrMmWaX_3khl8eLh8WZ4M_NgELqk5JoSUt9EQhitC5KHZ16MR2hGS94UjDfV8QHX9Sk6i3FDSNUKWs5QetUBYHDDCnuLt_u4Dt5vI-6cVzq5T5fGW7xzGlRSw-B7_KUidgaG5KwDgzNTBzHgnBPAuHzOEKc1YB8MBLxQQ1qrAH2veojn6MSqPsLF756j94f7t8VTsXx5fF7cLQvNqUiF7lhXWmWt4UaQDqypW11rwoAKVZqmM7zuWltqsKJiXHMmhLZGU9s1UNmaz9HVlLsL_mMPMcmN34chv5S0FWVLy4bRrKKTSgcfYwArd8FtVRglJfKnXDmVK3O58qdcOWYPmzwxa4cVhD_J_5q-AVDHgPY</recordid><startdate>20180501</startdate><enddate>20180501</enddate><creator>Vasdekis, Efstathios P.</creator><creator>Karkabounas, 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of mushrooms bioactivity: piceatannol was identified as a bioactive ingredient in the order Cantharellales</title><author>Vasdekis, Efstathios P. ; Karkabounas, Athanassios ; Giannakopoulos, Ioannis ; Savvas, Dimitrios ; Lekka, Marilena E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-cb2b4faffd3d60befd79c7c02e16a4d8bd37b9f4cef6523c3266cfdc1fb8e5f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adenocarcinoma</topic><topic>Agriculture</topic><topic>Analytical Chemistry</topic><topic>Antioxidants</topic><topic>Apoptosis</topic><topic>Bioactive compounds</topic><topic>Biocompatibility</topic><topic>Biological activity</topic><topic>Biotechnology</topic><topic>Cantharellales</topic><topic>Cantharellus</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Cytometry</topic><topic>Cytotoxicity</topic><topic>Flavonoids</topic><topic>Flow 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Collection</collection><collection>ProQuest Central Basic</collection><jtitle>European food research & technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vasdekis, Efstathios P.</au><au>Karkabounas, Athanassios</au><au>Giannakopoulos, Ioannis</au><au>Savvas, Dimitrios</au><au>Lekka, Marilena E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Screening of mushrooms bioactivity: piceatannol was identified as a bioactive ingredient in the order Cantharellales</atitle><jtitle>European food research & technology</jtitle><stitle>Eur Food Res Technol</stitle><date>2018-05-01</date><risdate>2018</risdate><volume>244</volume><issue>5</issue><spage>861</spage><epage>871</epage><pages>861-871</pages><issn>1438-2377</issn><eissn>1438-2385</eissn><abstract>Wild edible mushroom species are appreciated for consumption due to their high nutritional value. The aim of the present study was to examine in vitro beneficial bioactivity of mushroom extracts and to investigate the molecular identity of the active ingredients. In this regard, methanol extracts of 29 different wild edible mushroom species, that are traditionally consumed by residents in the National Park of North Pindos in North-Western Greece, were examined for antioxidant, antiproliferative, cytotoxic, and pro-apoptotic activities towards a human lung adenocarcinoma cell line A549 by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and by flow cytometry. Certain mushroom species exhibited high antioxidant activity, which was related to their high content in total phenols and flavonoids. Methanol extracts of
Cantharellus cibarius, Cantharellus cinereus, Craterellus cornucopioides
and
Hydnum repandum
, which belong to the order Cantharellales, exhibited high cytotoxicity and induced apoptosis–necrosis to A549 cells. High Performance Liquid Chromatography (HPLC) coupled with Mass Spectrometry analysis revealed as an active ingredient piceatannol ((E)-4-[2-(3,5-dihydroxyphenyl)ethenyl]1,2-benzenediol-3,3′,4,5′-tetrahydroxy-
trans
-stilbene). Piceatannol, according to our best knowledge, is identified for the first time in wild edible mushrooms. Experiments with authentic piceatannol confirmed the potent antiproliferative activity of this compound. Tested mushrooms are promising sources of bioactive compounds.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s00217-017-3007-y</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-8667-4927</orcidid></addata></record> |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Adenocarcinoma Agriculture Analytical Chemistry Antioxidants Apoptosis Bioactive compounds Biocompatibility Biological activity Biotechnology Cantharellales Cantharellus Chemistry Chemistry and Materials Science Cytometry Cytotoxicity Flavonoids Flow cytometry Food Science Forestry High performance liquid chromatography Liquid chromatography Lungs Mass spectrometry Mass spectroscopy Methanol Mushrooms National parks Nutritive value Original Paper Phenols Piceatannol Species Stilbene Toxicity trans-Stilbene |
| title | Screening of mushrooms bioactivity: piceatannol was identified as a bioactive ingredient in the order Cantharellales |
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