Effects of a long-term rearing system for deep-sea vesicomyid clams on host survival and endosymbiont retention
Deep-sea vesicomyid clams, including the genus Phreagena , harbor obligate sulfur-oxidizing symbiotic bacteria in gill epithelial cells. Difficulty in maintaining Phreagena clams in rearing tanks has been a major obstacle to achieving a better understanding of their unique biology. To improve the me...
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| Veröffentlicht in: | Fisheries science 2018-01, Vol.84 (1), p.41-51 |
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| creator | Ikuta, Tetsuro Sugimura, Makoto Nemoto, Suguru Aoki, Yui Tame, Akihiro Yamamoto, Masahiro Saito, Masaki Shimokawa, Yoshiki Miwa, Tetsuya Nagai, Yukiko Yoshida, Takao Fujikura, Katsunori Toyofuku, Takashi |
| description | Deep-sea vesicomyid clams, including the genus
Phreagena
, harbor obligate sulfur-oxidizing symbiotic bacteria in gill epithelial cells. Difficulty in maintaining
Phreagena
clams in rearing tanks has been a major obstacle to achieving a better understanding of their unique biology. To improve the method of rearing
Phreagena
clams, here we reared them in an artificial chemosynthetic aquarium and evaluated the effects of the aquarium system on long-term clam rearing. We compared the survival of clams reared in the artificial chemosynthetic tank with the survival of those in the normal tank, and analyzed the symbiont abundance using semi-quantification of fluorescent in situ hybridization signals. Our results indicate that the artificial chemosynthetic aquarium system had specific effects on symbiont abundance and possibly on host survival. Furthermore, transmission electron microscopic observations of sulfur globules in the symbiont cells and expression analyses of the
dsrA
gene of the symbiont indicated that stocked elemental sulfur could be consumed as an energy source to reduce sulfide shortages. We discuss the importance of higher and more stable sulfide concentrations and the proportions of available O
2
and CO
2
in driving appropriate metabolic functions of the symbiont and improving the survival of the clams. |
| doi_str_mv | 10.1007/s12562-017-1149-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1963782577</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1963782577</sourcerecordid><originalsourceid>FETCH-LOGICAL-c452t-572023379c94bdea9a7673130aa9b23303d8aa04958d43895b6706cc6145e9383</originalsourceid><addsrcrecordid>eNp1kE1LxDAURYMoOI7-AHcB19F8p1nK4BcMuNF1SNN07NA2NckM9N-boS7cuHoP3j33wQHgluB7grF6SIQKSREmChHCNaJnYEU454hqLM_BCmuikaayugRXKe0xxlLgagXCU9t6lxMMLbSwD-MOZR8HGL2N3biDaU7ZD7ANETbeTyh5C48-dS4Mc9dA19uhsCP8CinDdIjH7mh7aMcG-rEJaR7qLoy51GU_5rJeg4vW9snf_M41-Hx--ti8ou37y9vmcYscFzQjoSimjCntNK8bb7VVUjHCsLW6LgfMmspazLWoGs4qLWqpsHROEi68ZhVbg7uld4rh--BTNvtwiGN5aYiWTFVUKFVSZEm5GFKKvjVT7AYbZ0OwOXk1i1dTvJqTV0MLQxcmTSdDPv5p_hf6AU5Fewg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1963782577</pqid></control><display><type>article</type><title>Effects of a long-term rearing system for deep-sea vesicomyid clams on host survival and endosymbiont retention</title><source>SpringerLink (Online service)</source><creator>Ikuta, Tetsuro ; Sugimura, Makoto ; Nemoto, Suguru ; Aoki, Yui ; Tame, Akihiro ; Yamamoto, Masahiro ; Saito, Masaki ; Shimokawa, Yoshiki ; Miwa, Tetsuya ; Nagai, Yukiko ; Yoshida, Takao ; Fujikura, Katsunori ; Toyofuku, Takashi</creator><creatorcontrib>Ikuta, Tetsuro ; Sugimura, Makoto ; Nemoto, Suguru ; Aoki, Yui ; Tame, Akihiro ; Yamamoto, Masahiro ; Saito, Masaki ; Shimokawa, Yoshiki ; Miwa, Tetsuya ; Nagai, Yukiko ; Yoshida, Takao ; Fujikura, Katsunori ; Toyofuku, Takashi</creatorcontrib><description>Deep-sea vesicomyid clams, including the genus
Phreagena
, harbor obligate sulfur-oxidizing symbiotic bacteria in gill epithelial cells. Difficulty in maintaining
Phreagena
clams in rearing tanks has been a major obstacle to achieving a better understanding of their unique biology. To improve the method of rearing
Phreagena
clams, here we reared them in an artificial chemosynthetic aquarium and evaluated the effects of the aquarium system on long-term clam rearing. We compared the survival of clams reared in the artificial chemosynthetic tank with the survival of those in the normal tank, and analyzed the symbiont abundance using semi-quantification of fluorescent in situ hybridization signals. Our results indicate that the artificial chemosynthetic aquarium system had specific effects on symbiont abundance and possibly on host survival. Furthermore, transmission electron microscopic observations of sulfur globules in the symbiont cells and expression analyses of the
dsrA
gene of the symbiont indicated that stocked elemental sulfur could be consumed as an energy source to reduce sulfide shortages. We discuss the importance of higher and more stable sulfide concentrations and the proportions of available O
2
and CO
2
in driving appropriate metabolic functions of the symbiont and improving the survival of the clams.</description><identifier>ISSN: 0919-9268</identifier><identifier>EISSN: 1444-2906</identifier><identifier>DOI: 10.1007/s12562-017-1149-2</identifier><language>eng</language><publisher>Tokyo: Springer Japan</publisher><subject>Abundance ; Aquaria ; Aquariums ; Bacteria ; Biology ; Biomedical and Life Sciences ; Carbon dioxide ; Clams ; Deep sea ; Deep water ; Disease transmission ; Energy sources ; Epithelial cells ; Fish & Wildlife Biology & Management ; Fluorescence ; Fluorescence in situ hybridization ; Food Science ; Freshwater & Marine Ecology ; Gene expression ; Globules ; Individual rearing ; Life Sciences ; Original Article ; Oxidation ; Shortages ; Sulfides ; Sulfur ; Sulphides ; Sulphur ; Survival ; Symbionts ; Tanks</subject><ispartof>Fisheries science, 2018-01, Vol.84 (1), p.41-51</ispartof><rights>The Author(s) 2017</rights><rights>Fisheries Science is a copyright of Springer, (2017). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-572023379c94bdea9a7673130aa9b23303d8aa04958d43895b6706cc6145e9383</citedby><cites>FETCH-LOGICAL-c452t-572023379c94bdea9a7673130aa9b23303d8aa04958d43895b6706cc6145e9383</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12562-017-1149-2$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12562-017-1149-2$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Ikuta, Tetsuro</creatorcontrib><creatorcontrib>Sugimura, Makoto</creatorcontrib><creatorcontrib>Nemoto, Suguru</creatorcontrib><creatorcontrib>Aoki, Yui</creatorcontrib><creatorcontrib>Tame, Akihiro</creatorcontrib><creatorcontrib>Yamamoto, Masahiro</creatorcontrib><creatorcontrib>Saito, Masaki</creatorcontrib><creatorcontrib>Shimokawa, Yoshiki</creatorcontrib><creatorcontrib>Miwa, Tetsuya</creatorcontrib><creatorcontrib>Nagai, Yukiko</creatorcontrib><creatorcontrib>Yoshida, Takao</creatorcontrib><creatorcontrib>Fujikura, Katsunori</creatorcontrib><creatorcontrib>Toyofuku, Takashi</creatorcontrib><title>Effects of a long-term rearing system for deep-sea vesicomyid clams on host survival and endosymbiont retention</title><title>Fisheries science</title><addtitle>Fish Sci</addtitle><description>Deep-sea vesicomyid clams, including the genus
Phreagena
, harbor obligate sulfur-oxidizing symbiotic bacteria in gill epithelial cells. Difficulty in maintaining
Phreagena
clams in rearing tanks has been a major obstacle to achieving a better understanding of their unique biology. To improve the method of rearing
Phreagena
clams, here we reared them in an artificial chemosynthetic aquarium and evaluated the effects of the aquarium system on long-term clam rearing. We compared the survival of clams reared in the artificial chemosynthetic tank with the survival of those in the normal tank, and analyzed the symbiont abundance using semi-quantification of fluorescent in situ hybridization signals. Our results indicate that the artificial chemosynthetic aquarium system had specific effects on symbiont abundance and possibly on host survival. Furthermore, transmission electron microscopic observations of sulfur globules in the symbiont cells and expression analyses of the
dsrA
gene of the symbiont indicated that stocked elemental sulfur could be consumed as an energy source to reduce sulfide shortages. We discuss the importance of higher and more stable sulfide concentrations and the proportions of available O
2
and CO
2
in driving appropriate metabolic functions of the symbiont and improving the survival of the clams.</description><subject>Abundance</subject><subject>Aquaria</subject><subject>Aquariums</subject><subject>Bacteria</subject><subject>Biology</subject><subject>Biomedical and Life Sciences</subject><subject>Carbon dioxide</subject><subject>Clams</subject><subject>Deep sea</subject><subject>Deep water</subject><subject>Disease transmission</subject><subject>Energy sources</subject><subject>Epithelial cells</subject><subject>Fish & Wildlife Biology & Management</subject><subject>Fluorescence</subject><subject>Fluorescence in situ hybridization</subject><subject>Food Science</subject><subject>Freshwater & Marine Ecology</subject><subject>Gene expression</subject><subject>Globules</subject><subject>Individual rearing</subject><subject>Life Sciences</subject><subject>Original Article</subject><subject>Oxidation</subject><subject>Shortages</subject><subject>Sulfides</subject><subject>Sulfur</subject><subject>Sulphides</subject><subject>Sulphur</subject><subject>Survival</subject><subject>Symbionts</subject><subject>Tanks</subject><issn>0919-9268</issn><issn>1444-2906</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1kE1LxDAURYMoOI7-AHcB19F8p1nK4BcMuNF1SNN07NA2NckM9N-boS7cuHoP3j33wQHgluB7grF6SIQKSREmChHCNaJnYEU454hqLM_BCmuikaayugRXKe0xxlLgagXCU9t6lxMMLbSwD-MOZR8HGL2N3biDaU7ZD7ANETbeTyh5C48-dS4Mc9dA19uhsCP8CinDdIjH7mh7aMcG-rEJaR7qLoy51GU_5rJeg4vW9snf_M41-Hx--ti8ou37y9vmcYscFzQjoSimjCntNK8bb7VVUjHCsLW6LgfMmspazLWoGs4qLWqpsHROEi68ZhVbg7uld4rh--BTNvtwiGN5aYiWTFVUKFVSZEm5GFKKvjVT7AYbZ0OwOXk1i1dTvJqTV0MLQxcmTSdDPv5p_hf6AU5Fewg</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Ikuta, 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of a long-term rearing system for deep-sea vesicomyid clams on host survival and endosymbiont retention</title><author>Ikuta, Tetsuro ; Sugimura, Makoto ; Nemoto, Suguru ; Aoki, Yui ; Tame, Akihiro ; Yamamoto, Masahiro ; Saito, Masaki ; Shimokawa, Yoshiki ; Miwa, Tetsuya ; Nagai, Yukiko ; Yoshida, Takao ; Fujikura, Katsunori ; Toyofuku, Takashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-572023379c94bdea9a7673130aa9b23303d8aa04958d43895b6706cc6145e9383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Abundance</topic><topic>Aquaria</topic><topic>Aquariums</topic><topic>Bacteria</topic><topic>Biology</topic><topic>Biomedical and Life Sciences</topic><topic>Carbon dioxide</topic><topic>Clams</topic><topic>Deep sea</topic><topic>Deep water</topic><topic>Disease transmission</topic><topic>Energy sources</topic><topic>Epithelial cells</topic><topic>Fish & Wildlife Biology & Management</topic><topic>Fluorescence</topic><topic>Fluorescence in situ hybridization</topic><topic>Food Science</topic><topic>Freshwater & Marine Ecology</topic><topic>Gene expression</topic><topic>Globules</topic><topic>Individual rearing</topic><topic>Life Sciences</topic><topic>Original Article</topic><topic>Oxidation</topic><topic>Shortages</topic><topic>Sulfides</topic><topic>Sulfur</topic><topic>Sulphides</topic><topic>Sulphur</topic><topic>Survival</topic><topic>Symbionts</topic><topic>Tanks</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ikuta, Tetsuro</creatorcontrib><creatorcontrib>Sugimura, Makoto</creatorcontrib><creatorcontrib>Nemoto, Suguru</creatorcontrib><creatorcontrib>Aoki, Yui</creatorcontrib><creatorcontrib>Tame, Akihiro</creatorcontrib><creatorcontrib>Yamamoto, Masahiro</creatorcontrib><creatorcontrib>Saito, Masaki</creatorcontrib><creatorcontrib>Shimokawa, Yoshiki</creatorcontrib><creatorcontrib>Miwa, Tetsuya</creatorcontrib><creatorcontrib>Nagai, Yukiko</creatorcontrib><creatorcontrib>Yoshida, Takao</creatorcontrib><creatorcontrib>Fujikura, Katsunori</creatorcontrib><creatorcontrib>Toyofuku, Takashi</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central 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Sci</stitle><date>2018-01-01</date><risdate>2018</risdate><volume>84</volume><issue>1</issue><spage>41</spage><epage>51</epage><pages>41-51</pages><issn>0919-9268</issn><eissn>1444-2906</eissn><abstract>Deep-sea vesicomyid clams, including the genus
Phreagena
, harbor obligate sulfur-oxidizing symbiotic bacteria in gill epithelial cells. Difficulty in maintaining
Phreagena
clams in rearing tanks has been a major obstacle to achieving a better understanding of their unique biology. To improve the method of rearing
Phreagena
clams, here we reared them in an artificial chemosynthetic aquarium and evaluated the effects of the aquarium system on long-term clam rearing. We compared the survival of clams reared in the artificial chemosynthetic tank with the survival of those in the normal tank, and analyzed the symbiont abundance using semi-quantification of fluorescent in situ hybridization signals. Our results indicate that the artificial chemosynthetic aquarium system had specific effects on symbiont abundance and possibly on host survival. Furthermore, transmission electron microscopic observations of sulfur globules in the symbiont cells and expression analyses of the
dsrA
gene of the symbiont indicated that stocked elemental sulfur could be consumed as an energy source to reduce sulfide shortages. We discuss the importance of higher and more stable sulfide concentrations and the proportions of available O
2
and CO
2
in driving appropriate metabolic functions of the symbiont and improving the survival of the clams.</abstract><cop>Tokyo</cop><pub>Springer Japan</pub><doi>10.1007/s12562-017-1149-2</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0919-9268 |
| ispartof | Fisheries science, 2018-01, Vol.84 (1), p.41-51 |
| issn | 0919-9268 1444-2906 |
| language | eng |
| recordid | cdi_proquest_journals_1963782577 |
| source | SpringerLink (Online service) |
| subjects | Abundance Aquaria Aquariums Bacteria Biology Biomedical and Life Sciences Carbon dioxide Clams Deep sea Deep water Disease transmission Energy sources Epithelial cells Fish & Wildlife Biology & Management Fluorescence Fluorescence in situ hybridization Food Science Freshwater & Marine Ecology Gene expression Globules Individual rearing Life Sciences Original Article Oxidation Shortages Sulfides Sulfur Sulphides Sulphur Survival Symbionts Tanks |
| title | Effects of a long-term rearing system for deep-sea vesicomyid clams on host survival and endosymbiont retention |
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