Viperidae snake venoms block nicotinic acetylcholine receptors and voltage-gated Ca2+ channels in identified neurons of fresh-water snail Lymnaea stagnalis
Snake venoms contain a vast array of toxic polypeptide components interacting with a variety of cell targets. Thus, Elapidae snake venoms contain α-neurotoxins with very high affinity for nicotinic acetylcholine receptors (nAChRs) and a few toxins able to suppress the activity of Ca 2+ and K + chann...
Gespeichert in:
| Veröffentlicht in: | Biochemistry (Moscow). Supplement series A, Membrane and cell biology Membrane and cell biology, 2008-03, Vol.2 (1), p.14-18 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 18 |
|---|---|
| container_issue | 1 |
| container_start_page | 14 |
| container_title | Biochemistry (Moscow). Supplement series A, Membrane and cell biology |
| container_volume | 2 |
| creator | Gorbacheva, E. V. Starkov, V. G. Tsetlin, V. I. Utkin, Yu. N. Vulfius, C. A. |
| description | Snake venoms contain a vast array of toxic polypeptide components interacting with a variety of cell targets. Thus,
Elapidae
snake venoms contain α-neurotoxins with very high affinity for nicotinic acetylcholine receptors (nAChRs) and a few toxins able to suppress the activity of Ca
2+
and K
+
channels. Experimental evidence for the presence of nAChR antagonists and voltage-gated ionic channel blockers in venoms of Viperidae snakes is very scarce. In this study, effects of crude venoms of seven snake species (
Vipera nikolskii, Echis multisquamatus, Gloydius saxatilis, Bitis arietans, Vipera renardi, Vipera lebetina
, and
Naja kaouthia
) on nAChRs and voltage-gated Ca
2+
channels were studied for the first time. The experiments were carried out on isolated identified neurons of the fresh-water mollusc
Lymnaea stagnalis
using voltage-clamp and intracellular perfusion techniques. All Viperidae snake venoms under study blocked nAChRs and voltage-gated Ca
2+
channels. The potency of these venoms against nAChRs was significantly lower in comparison with
N. kaouthia
venom which is rich of α-neurotoxins; however, the extent of Ca
2+
channel block by venoms of Viperidae snakes and
N. kaouthia
was similar. The data obtained suggest that Viperidae snake venoms tested in this study contain peptides with affinity both for nAChRs and for voltage-gated Ca
2+
channels. |
| doi_str_mv | 10.1007/s11827-008-1003-x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_sprin</sourceid><recordid>TN_cdi_proquest_journals_196349657</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1897773521</sourcerecordid><originalsourceid>FETCH-LOGICAL-p70x-d2b7cc2271119dbdbd40e5152169031bc9d2e0093971f0ba375e35ce780652ec3</originalsourceid><addsrcrecordid>eNpNUdtKAzEQDaJgrX6Ab8FXieayu2kepXiDgi_F1yWbnW3TpsmabLX9Fn_WlIrIwFyYM2eGOQhdM3rHKJX3ibEJl4TSCcm1ILsTNGJKUSILVZz-5XJyji5SWlFaiaKqRuj73fYQbasBJ6_XgD_Bh03CjQtmjb01YbDZY21g2DuzDM56wBEM9EOICWvf4s_gBr0AstADtHiq-S02S-09uIStx7YFP9jO5p6HbQw-4dDhLkJakq88Eg-brcOz_cZr0DhlMq-dTZforNMuwdVvHKP50-N8-kJmb8-v04cZ6SXdkZY30hjOJWNMtU22gkLJSs4qRQVrjGo5UKqEkqyjjRayBFEakBNalRyMGKObI20fw8cW0lCvwjbmC1LNVH6TqkqZQfwISn20fgHxH4jWBwnqowR1luBQi3onfgBI1H00</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>196349657</pqid></control><display><type>article</type><title>Viperidae snake venoms block nicotinic acetylcholine receptors and voltage-gated Ca2+ channels in identified neurons of fresh-water snail Lymnaea stagnalis</title><source>Springer Nature - Complete Springer Journals</source><creator>Gorbacheva, E. V. ; Starkov, V. G. ; Tsetlin, V. I. ; Utkin, Yu. N. ; Vulfius, C. A.</creator><creatorcontrib>Gorbacheva, E. V. ; Starkov, V. G. ; Tsetlin, V. I. ; Utkin, Yu. N. ; Vulfius, C. A.</creatorcontrib><description>Snake venoms contain a vast array of toxic polypeptide components interacting with a variety of cell targets. Thus,
Elapidae
snake venoms contain α-neurotoxins with very high affinity for nicotinic acetylcholine receptors (nAChRs) and a few toxins able to suppress the activity of Ca
2+
and K
+
channels. Experimental evidence for the presence of nAChR antagonists and voltage-gated ionic channel blockers in venoms of Viperidae snakes is very scarce. In this study, effects of crude venoms of seven snake species (
Vipera nikolskii, Echis multisquamatus, Gloydius saxatilis, Bitis arietans, Vipera renardi, Vipera lebetina
, and
Naja kaouthia
) on nAChRs and voltage-gated Ca
2+
channels were studied for the first time. The experiments were carried out on isolated identified neurons of the fresh-water mollusc
Lymnaea stagnalis
using voltage-clamp and intracellular perfusion techniques. All Viperidae snake venoms under study blocked nAChRs and voltage-gated Ca
2+
channels. The potency of these venoms against nAChRs was significantly lower in comparison with
N. kaouthia
venom which is rich of α-neurotoxins; however, the extent of Ca
2+
channel block by venoms of Viperidae snakes and
N. kaouthia
was similar. The data obtained suggest that Viperidae snake venoms tested in this study contain peptides with affinity both for nAChRs and for voltage-gated Ca
2+
channels.</description><identifier>ISSN: 1990-7478</identifier><identifier>EISSN: 1990-7494</identifier><identifier>DOI: 10.1007/s11827-008-1003-x</identifier><language>eng</language><publisher>Moscow: Nauka/Interperiodica</publisher><subject>Biochemistry ; Biomedical and Life Sciences ; Cell Biology ; Life Sciences ; Mollusks ; Neurons ; Peptides ; Snakes ; Toxicology</subject><ispartof>Biochemistry (Moscow). Supplement series A, Membrane and cell biology, 2008-03, Vol.2 (1), p.14-18</ispartof><rights>Pleiades Publishing, Ltd. 2008</rights><rights>MAIK Nauka 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-p70x-d2b7cc2271119dbdbd40e5152169031bc9d2e0093971f0ba375e35ce780652ec3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11827-008-1003-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11827-008-1003-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27911,27912,41475,42544,51306</link.rule.ids></links><search><creatorcontrib>Gorbacheva, E. V.</creatorcontrib><creatorcontrib>Starkov, V. G.</creatorcontrib><creatorcontrib>Tsetlin, V. I.</creatorcontrib><creatorcontrib>Utkin, Yu. N.</creatorcontrib><creatorcontrib>Vulfius, C. A.</creatorcontrib><title>Viperidae snake venoms block nicotinic acetylcholine receptors and voltage-gated Ca2+ channels in identified neurons of fresh-water snail Lymnaea stagnalis</title><title>Biochemistry (Moscow). Supplement series A, Membrane and cell biology</title><addtitle>Biochem. Moscow Suppl. Ser. A</addtitle><description>Snake venoms contain a vast array of toxic polypeptide components interacting with a variety of cell targets. Thus,
Elapidae
snake venoms contain α-neurotoxins with very high affinity for nicotinic acetylcholine receptors (nAChRs) and a few toxins able to suppress the activity of Ca
2+
and K
+
channels. Experimental evidence for the presence of nAChR antagonists and voltage-gated ionic channel blockers in venoms of Viperidae snakes is very scarce. In this study, effects of crude venoms of seven snake species (
Vipera nikolskii, Echis multisquamatus, Gloydius saxatilis, Bitis arietans, Vipera renardi, Vipera lebetina
, and
Naja kaouthia
) on nAChRs and voltage-gated Ca
2+
channels were studied for the first time. The experiments were carried out on isolated identified neurons of the fresh-water mollusc
Lymnaea stagnalis
using voltage-clamp and intracellular perfusion techniques. All Viperidae snake venoms under study blocked nAChRs and voltage-gated Ca
2+
channels. The potency of these venoms against nAChRs was significantly lower in comparison with
N. kaouthia
venom which is rich of α-neurotoxins; however, the extent of Ca
2+
channel block by venoms of Viperidae snakes and
N. kaouthia
was similar. The data obtained suggest that Viperidae snake venoms tested in this study contain peptides with affinity both for nAChRs and for voltage-gated Ca
2+
channels.</description><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>Life Sciences</subject><subject>Mollusks</subject><subject>Neurons</subject><subject>Peptides</subject><subject>Snakes</subject><subject>Toxicology</subject><issn>1990-7478</issn><issn>1990-7494</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpNUdtKAzEQDaJgrX6Ab8FXieayu2kepXiDgi_F1yWbnW3TpsmabLX9Fn_WlIrIwFyYM2eGOQhdM3rHKJX3ibEJl4TSCcm1ILsTNGJKUSILVZz-5XJyji5SWlFaiaKqRuj73fYQbasBJ6_XgD_Bh03CjQtmjb01YbDZY21g2DuzDM56wBEM9EOICWvf4s_gBr0AstADtHiq-S02S-09uIStx7YFP9jO5p6HbQw-4dDhLkJakq88Eg-brcOz_cZr0DhlMq-dTZforNMuwdVvHKP50-N8-kJmb8-v04cZ6SXdkZY30hjOJWNMtU22gkLJSs4qRQVrjGo5UKqEkqyjjRayBFEakBNalRyMGKObI20fw8cW0lCvwjbmC1LNVH6TqkqZQfwISn20fgHxH4jWBwnqowR1luBQi3onfgBI1H00</recordid><startdate>20080301</startdate><enddate>20080301</enddate><creator>Gorbacheva, E. V.</creator><creator>Starkov, V. G.</creator><creator>Tsetlin, V. I.</creator><creator>Utkin, Yu. N.</creator><creator>Vulfius, C. A.</creator><general>Nauka/Interperiodica</general><general>Springer Nature B.V</general><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88I</scope><scope>8AO</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>LK8</scope><scope>M0S</scope><scope>M2P</scope><scope>M7P</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope></search><sort><creationdate>20080301</creationdate><title>Viperidae snake venoms block nicotinic acetylcholine receptors and voltage-gated Ca2+ channels in identified neurons of fresh-water snail Lymnaea stagnalis</title><author>Gorbacheva, E. V. ; Starkov, V. G. ; Tsetlin, V. I. ; Utkin, Yu. N. ; Vulfius, C. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p70x-d2b7cc2271119dbdbd40e5152169031bc9d2e0093971f0ba375e35ce780652ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Cell Biology</topic><topic>Life Sciences</topic><topic>Mollusks</topic><topic>Neurons</topic><topic>Peptides</topic><topic>Snakes</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gorbacheva, E. V.</creatorcontrib><creatorcontrib>Starkov, V. G.</creatorcontrib><creatorcontrib>Tsetlin, V. I.</creatorcontrib><creatorcontrib>Utkin, Yu. N.</creatorcontrib><creatorcontrib>Vulfius, C. A.</creatorcontrib><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><jtitle>Biochemistry (Moscow). Supplement series A, Membrane and cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gorbacheva, E. V.</au><au>Starkov, V. G.</au><au>Tsetlin, V. I.</au><au>Utkin, Yu. N.</au><au>Vulfius, C. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Viperidae snake venoms block nicotinic acetylcholine receptors and voltage-gated Ca2+ channels in identified neurons of fresh-water snail Lymnaea stagnalis</atitle><jtitle>Biochemistry (Moscow). Supplement series A, Membrane and cell biology</jtitle><stitle>Biochem. Moscow Suppl. Ser. A</stitle><date>2008-03-01</date><risdate>2008</risdate><volume>2</volume><issue>1</issue><spage>14</spage><epage>18</epage><pages>14-18</pages><issn>1990-7478</issn><eissn>1990-7494</eissn><abstract>Snake venoms contain a vast array of toxic polypeptide components interacting with a variety of cell targets. Thus,
Elapidae
snake venoms contain α-neurotoxins with very high affinity for nicotinic acetylcholine receptors (nAChRs) and a few toxins able to suppress the activity of Ca
2+
and K
+
channels. Experimental evidence for the presence of nAChR antagonists and voltage-gated ionic channel blockers in venoms of Viperidae snakes is very scarce. In this study, effects of crude venoms of seven snake species (
Vipera nikolskii, Echis multisquamatus, Gloydius saxatilis, Bitis arietans, Vipera renardi, Vipera lebetina
, and
Naja kaouthia
) on nAChRs and voltage-gated Ca
2+
channels were studied for the first time. The experiments were carried out on isolated identified neurons of the fresh-water mollusc
Lymnaea stagnalis
using voltage-clamp and intracellular perfusion techniques. All Viperidae snake venoms under study blocked nAChRs and voltage-gated Ca
2+
channels. The potency of these venoms against nAChRs was significantly lower in comparison with
N. kaouthia
venom which is rich of α-neurotoxins; however, the extent of Ca
2+
channel block by venoms of Viperidae snakes and
N. kaouthia
was similar. The data obtained suggest that Viperidae snake venoms tested in this study contain peptides with affinity both for nAChRs and for voltage-gated Ca
2+
channels.</abstract><cop>Moscow</cop><pub>Nauka/Interperiodica</pub><doi>10.1007/s11827-008-1003-x</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1990-7478 |
| ispartof | Biochemistry (Moscow). Supplement series A, Membrane and cell biology, 2008-03, Vol.2 (1), p.14-18 |
| issn | 1990-7478 1990-7494 |
| language | eng |
| recordid | cdi_proquest_journals_196349657 |
| source | Springer Nature - Complete Springer Journals |
| subjects | Biochemistry Biomedical and Life Sciences Cell Biology Life Sciences Mollusks Neurons Peptides Snakes Toxicology |
| title | Viperidae snake venoms block nicotinic acetylcholine receptors and voltage-gated Ca2+ channels in identified neurons of fresh-water snail Lymnaea stagnalis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T21%3A27%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_sprin&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Viperidae%20snake%20venoms%20block%20nicotinic%20acetylcholine%20receptors%20and%20voltage-gated%20Ca2+%20channels%20in%20identified%20neurons%20of%20fresh-water%20snail%20Lymnaea%20stagnalis&rft.jtitle=Biochemistry%20(Moscow).%20Supplement%20series%20A,%20Membrane%20and%20cell%20biology&rft.au=Gorbacheva,%20E.%20V.&rft.date=2008-03-01&rft.volume=2&rft.issue=1&rft.spage=14&rft.epage=18&rft.pages=14-18&rft.issn=1990-7478&rft.eissn=1990-7494&rft_id=info:doi/10.1007/s11827-008-1003-x&rft_dat=%3Cproquest_sprin%3E1897773521%3C/proquest_sprin%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=196349657&rft_id=info:pmid/&rfr_iscdi=true |