effect of chloramphenicol, actinomycin D and 5-bromouracil of the synthesis of photosynthetic pigments
The present study concerned the effect of chloramphenicol (100 μg/ml), actinomycin D (30 μg/ml), and 5-bromouracil (190 μg/ml) on the accumulation of chlorophyll α, chlorophyll b, β-carotene and four fractions of xanthophylls (with the domination of: lutein, zeaxanthin, violaxanthin and neoxanthin)...
Gespeichert in:
| Veröffentlicht in: | Acta Societatis Botanicorum Poloniae 2015-01, Vol.43 (2), p.251-259 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 259 |
|---|---|
| container_issue | 2 |
| container_start_page | 251 |
| container_title | Acta Societatis Botanicorum Poloniae |
| container_volume | 43 |
| creator | Ficek, S Wieckowski, S |
| description | The present study concerned the effect of chloramphenicol (100 μg/ml), actinomycin D (30 μg/ml), and 5-bromouracil (190 μg/ml) on the accumulation of chlorophyll α, chlorophyll b, β-carotene and four fractions of xanthophylls (with the domination of: lutein, zeaxanthin, violaxanthin and neoxanthin) in the primary bean leaves. The pigment content was determined in etiolated leaves after exposure to light for different lengths of time. It results from this study that chloramphenicol inhibits β-carotene synthesis more than do other pigments. The formation of xanthophylls and chlorophyll b is relatively less sensitive to the action of this antibiotic. Actinomycin D is also a somewhat more effective inhibitor of the accumulation of β-carotene than other pigments. In 5-bromouracil-treated leaves the accumulation of all carotenoids is inhibited almost to the same extent. These results suggest that the accumulation of chlorophyll b and xanthophylls is a little less dependent upon the activity of 70 S ribosomes in chloroplasts than the accumulation of chlorophyll α and β-carotene. |
| doi_str_mv | 10.5586/asbp.1974.024 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_proquest_journals_1962404215</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_e8d8e48139004c3b9ac2e24761b989f7</doaj_id><sourcerecordid>1962404215</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1565-56604136cbaa91d946f42773ffa5d13d8a07a7c257546093c5f03824c4dda6ef3</originalsourceid><addsrcrecordid>eNo9kc1v1DAQxS0EEqvSI2cicSXL-Ds-ovJVqVIPpWdr4ti7XiVxsNPD_vc4BHEazdNv3szoEfKewlHKTn3G0i9HarQ4AhOvyIFBx1sjOnhNDgBAW2W0fktuS7nUFiSAkfpAgg_Bu7VJoXHnMWWclrOfo0vjpwbdGuc0XV2cm68NzkMj2z6nKb1kdHHcZtazb8p1rqXEsgnLOa1pV9bomiWeJj-v5R15E3As_vZfvSHP37_9uvvZPjz-uL_78tA6KpVspVIgKFeuRzR0MEIFwbTmIaAcKB86BI3aMamlUGC4kwF4x4QTw4DKB35D7nffIeHFLjlOmK82YbR_hZRPFnM9bPTWd0PnRUe5ARCO9wYd80xoRXvTmaCr18fda8np94svq73Uz-d6vqVGMQGCUVmpdqdcTqVkH_5vpWC3ZOyWjN2SsTWZyn_Y-YDJ4inHYp-fGFAO9VElKvEHVyKKPw</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1962404215</pqid></control><display><type>article</type><title>effect of chloramphenicol, actinomycin D and 5-bromouracil of the synthesis of photosynthetic pigments</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Ficek, S ; Wieckowski, S</creator><creatorcontrib>Ficek, S ; Wieckowski, S</creatorcontrib><description>The present study concerned the effect of chloramphenicol (100 μg/ml), actinomycin D (30 μg/ml), and 5-bromouracil (190 μg/ml) on the accumulation of chlorophyll α, chlorophyll b, β-carotene and four fractions of xanthophylls (with the domination of: lutein, zeaxanthin, violaxanthin and neoxanthin) in the primary bean leaves. The pigment content was determined in etiolated leaves after exposure to light for different lengths of time. It results from this study that chloramphenicol inhibits β-carotene synthesis more than do other pigments. The formation of xanthophylls and chlorophyll b is relatively less sensitive to the action of this antibiotic. Actinomycin D is also a somewhat more effective inhibitor of the accumulation of β-carotene than other pigments. In 5-bromouracil-treated leaves the accumulation of all carotenoids is inhibited almost to the same extent. These results suggest that the accumulation of chlorophyll b and xanthophylls is a little less dependent upon the activity of 70 S ribosomes in chloroplasts than the accumulation of chlorophyll α and β-carotene.</description><identifier>ISSN: 0001-6977</identifier><identifier>ISSN: 2083-9480</identifier><identifier>EISSN: 2083-9480</identifier><identifier>DOI: 10.5586/asbp.1974.024</identifier><language>eng</language><publisher>Warsaw: Polish Botanical Society</publisher><subject>Carotenoids ; Chloramphenicol ; Chlorophyll ; Chloroplasts ; Glucose ; horticultural crops ; Leaves ; Photosynthetic pigments ; Pigments ; plant biochemistry ; plant physiology ; Ribosomes ; Violaxanthin ; Zeaxanthin ; β-Carotene</subject><ispartof>Acta Societatis Botanicorum Poloniae, 2015-01, Vol.43 (2), p.251-259</ispartof><rights>Copyright (c) 2015 S. Ficek, S. Więckowski. This work is licensed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0 (the “License”). Notwithstanding the ProQuest Terms and conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids></links><search><creatorcontrib>Ficek, S</creatorcontrib><creatorcontrib>Wieckowski, S</creatorcontrib><title>effect of chloramphenicol, actinomycin D and 5-bromouracil of the synthesis of photosynthetic pigments</title><title>Acta Societatis Botanicorum Poloniae</title><description>The present study concerned the effect of chloramphenicol (100 μg/ml), actinomycin D (30 μg/ml), and 5-bromouracil (190 μg/ml) on the accumulation of chlorophyll α, chlorophyll b, β-carotene and four fractions of xanthophylls (with the domination of: lutein, zeaxanthin, violaxanthin and neoxanthin) in the primary bean leaves. The pigment content was determined in etiolated leaves after exposure to light for different lengths of time. It results from this study that chloramphenicol inhibits β-carotene synthesis more than do other pigments. The formation of xanthophylls and chlorophyll b is relatively less sensitive to the action of this antibiotic. Actinomycin D is also a somewhat more effective inhibitor of the accumulation of β-carotene than other pigments. In 5-bromouracil-treated leaves the accumulation of all carotenoids is inhibited almost to the same extent. These results suggest that the accumulation of chlorophyll b and xanthophylls is a little less dependent upon the activity of 70 S ribosomes in chloroplasts than the accumulation of chlorophyll α and β-carotene.</description><subject>Carotenoids</subject><subject>Chloramphenicol</subject><subject>Chlorophyll</subject><subject>Chloroplasts</subject><subject>Glucose</subject><subject>horticultural crops</subject><subject>Leaves</subject><subject>Photosynthetic pigments</subject><subject>Pigments</subject><subject>plant biochemistry</subject><subject>plant physiology</subject><subject>Ribosomes</subject><subject>Violaxanthin</subject><subject>Zeaxanthin</subject><subject>β-Carotene</subject><issn>0001-6977</issn><issn>2083-9480</issn><issn>2083-9480</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNo9kc1v1DAQxS0EEqvSI2cicSXL-Ds-ovJVqVIPpWdr4ti7XiVxsNPD_vc4BHEazdNv3szoEfKewlHKTn3G0i9HarQ4AhOvyIFBx1sjOnhNDgBAW2W0fktuS7nUFiSAkfpAgg_Bu7VJoXHnMWWclrOfo0vjpwbdGuc0XV2cm68NzkMj2z6nKb1kdHHcZtazb8p1rqXEsgnLOa1pV9bomiWeJj-v5R15E3As_vZfvSHP37_9uvvZPjz-uL_78tA6KpVspVIgKFeuRzR0MEIFwbTmIaAcKB86BI3aMamlUGC4kwF4x4QTw4DKB35D7nffIeHFLjlOmK82YbR_hZRPFnM9bPTWd0PnRUe5ARCO9wYd80xoRXvTmaCr18fda8np94svq73Uz-d6vqVGMQGCUVmpdqdcTqVkH_5vpWC3ZOyWjN2SsTWZyn_Y-YDJ4inHYp-fGFAO9VElKvEHVyKKPw</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Ficek, S</creator><creator>Wieckowski, S</creator><general>Polish Botanical Society</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>DOA</scope></search><sort><creationdate>20150101</creationdate><title>effect of chloramphenicol, actinomycin D and 5-bromouracil of the synthesis of photosynthetic pigments</title><author>Ficek, S ; Wieckowski, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1565-56604136cbaa91d946f42773ffa5d13d8a07a7c257546093c5f03824c4dda6ef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Carotenoids</topic><topic>Chloramphenicol</topic><topic>Chlorophyll</topic><topic>Chloroplasts</topic><topic>Glucose</topic><topic>horticultural crops</topic><topic>Leaves</topic><topic>Photosynthetic pigments</topic><topic>Pigments</topic><topic>plant biochemistry</topic><topic>plant physiology</topic><topic>Ribosomes</topic><topic>Violaxanthin</topic><topic>Zeaxanthin</topic><topic>β-Carotene</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ficek, S</creatorcontrib><creatorcontrib>Wieckowski, S</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Acta Societatis Botanicorum Poloniae</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ficek, S</au><au>Wieckowski, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>effect of chloramphenicol, actinomycin D and 5-bromouracil of the synthesis of photosynthetic pigments</atitle><jtitle>Acta Societatis Botanicorum Poloniae</jtitle><date>2015-01-01</date><risdate>2015</risdate><volume>43</volume><issue>2</issue><spage>251</spage><epage>259</epage><pages>251-259</pages><issn>0001-6977</issn><issn>2083-9480</issn><eissn>2083-9480</eissn><abstract>The present study concerned the effect of chloramphenicol (100 μg/ml), actinomycin D (30 μg/ml), and 5-bromouracil (190 μg/ml) on the accumulation of chlorophyll α, chlorophyll b, β-carotene and four fractions of xanthophylls (with the domination of: lutein, zeaxanthin, violaxanthin and neoxanthin) in the primary bean leaves. The pigment content was determined in etiolated leaves after exposure to light for different lengths of time. It results from this study that chloramphenicol inhibits β-carotene synthesis more than do other pigments. The formation of xanthophylls and chlorophyll b is relatively less sensitive to the action of this antibiotic. Actinomycin D is also a somewhat more effective inhibitor of the accumulation of β-carotene than other pigments. In 5-bromouracil-treated leaves the accumulation of all carotenoids is inhibited almost to the same extent. These results suggest that the accumulation of chlorophyll b and xanthophylls is a little less dependent upon the activity of 70 S ribosomes in chloroplasts than the accumulation of chlorophyll α and β-carotene.</abstract><cop>Warsaw</cop><pub>Polish Botanical Society</pub><doi>10.5586/asbp.1974.024</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0001-6977 |
| ispartof | Acta Societatis Botanicorum Poloniae, 2015-01, Vol.43 (2), p.251-259 |
| issn | 0001-6977 2083-9480 2083-9480 |
| language | eng |
| recordid | cdi_proquest_journals_1962404215 |
| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Carotenoids Chloramphenicol Chlorophyll Chloroplasts Glucose horticultural crops Leaves Photosynthetic pigments Pigments plant biochemistry plant physiology Ribosomes Violaxanthin Zeaxanthin β-Carotene |
| title | effect of chloramphenicol, actinomycin D and 5-bromouracil of the synthesis of photosynthetic pigments |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T17%3A53%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=effect%20of%20chloramphenicol,%20actinomycin%20D%20and%205-bromouracil%20of%20the%20synthesis%20of%20photosynthetic%20pigments&rft.jtitle=Acta%20Societatis%20Botanicorum%20Poloniae&rft.au=Ficek,%20S&rft.date=2015-01-01&rft.volume=43&rft.issue=2&rft.spage=251&rft.epage=259&rft.pages=251-259&rft.issn=0001-6977&rft.eissn=2083-9480&rft_id=info:doi/10.5586/asbp.1974.024&rft_dat=%3Cproquest_doaj_%3E1962404215%3C/proquest_doaj_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1962404215&rft_id=info:pmid/&rft_doaj_id=oai_doaj_org_article_e8d8e48139004c3b9ac2e24761b989f7&rfr_iscdi=true |