Albizia harveyi: phytochemical profiling, antioxidant, antidiabetic and hepatoprotective activities of the bark extract
Profiling the polyphenols in the methanol extract from the bark of Albizia harveyi was performed by HPLC–PDA–ESI–MS/MS analysis. The phytochemical analysis identified 39 compounds, the majority of them were flavan-3-ol derivatives and condensed tannins. Total phenolic content, determined by the Foli...
Gespeichert in:
| Veröffentlicht in: | Medicinal chemistry research 2017-12, Vol.26 (12), p.3091-3105 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3105 |
|---|---|
| container_issue | 12 |
| container_start_page | 3091 |
| container_title | Medicinal chemistry research |
| container_volume | 26 |
| creator | Sobeh, Mansour Mahmoud, Mona F. Abdelfattah, Mohamed A. O. El-Beshbishy, Hesham A. El-Shazly, Assem M. Wink, Michael |
| description | Profiling the polyphenols in the methanol extract from the bark of
Albizia harveyi
was performed by HPLC–PDA–ESI–MS/MS analysis. The phytochemical analysis identified 39 compounds, the majority of them were flavan-3-ol derivatives and condensed tannins. Total phenolic content, determined by the Folin–Ciocalteu method amounted to 489 mg gallic acid equivalents/g extract. The extract showed promising antioxidant activities with an EC
50
of 3.6 µg/mL and 18.32 mM FeSO
4
equivalent/mg extract in radical scavenging assay and ferric reducing antioxidant power assays, respectively. The hepatoprotective potential of the extract in rats was determined in vivo in a
d-
galactosamine-induced liver toxicity model. A dose of 100 mg/kg (body weight) of the bark extract reduced levels of aspartate aminotransferase, gamma-glutamyltransferase and total bilirubin by 35.7, 65.3, and 23.8% (
p
|
| doi_str_mv | 10.1007/s00044-017-2005-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1961473428</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1961473428</sourcerecordid><originalsourceid>FETCH-LOGICAL-c316t-624448b47a7451f8717bf1692fb5259e896359f8347e2e600f027d027b2135d43</originalsourceid><addsrcrecordid>eNp1UMtOwzAQtBBIlMIHcLPElcDaseOEW1XxkpC4wNlyErtxSZNgu6Xl63EUDlw4rGZXOzO7GoQuCdwQAHHrAYCxBIhIKABP8iM0I5yzJCcUjmMPsaecpqfozPs1QCqA8Rn6WrSl_bYKN8rt9MHe4aE5hL5q9MZWqsWD641tbbe6xqoLtt_bOuI01FaVOtgqDjVu9KBCH-lBV8HuNFYj2GC1x73BodG4VO4D631wcXWOToxqvb74xTl6f7h_Wz4lL6-Pz8vFS1KlJAtJRhljecmEEowTkwsiSkOygpqSU17ovMhSXpg8ZUJTnQEYoKKOVVKS8pqlc3Q1-cbPPrfaB7nut66LJyUpMsJEymgeWWRiVa733mkjB2c3yh0kATnmK6d8ZcxXjvnKUUMnjY_cbqXdH-d_RT9gCX4N</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1961473428</pqid></control><display><type>article</type><title>Albizia harveyi: phytochemical profiling, antioxidant, antidiabetic and hepatoprotective activities of the bark extract</title><source>SpringerNature Journals</source><creator>Sobeh, Mansour ; Mahmoud, Mona F. ; Abdelfattah, Mohamed A. O. ; El-Beshbishy, Hesham A. ; El-Shazly, Assem M. ; Wink, Michael</creator><creatorcontrib>Sobeh, Mansour ; Mahmoud, Mona F. ; Abdelfattah, Mohamed A. O. ; El-Beshbishy, Hesham A. ; El-Shazly, Assem M. ; Wink, Michael</creatorcontrib><description>Profiling the polyphenols in the methanol extract from the bark of
Albizia harveyi
was performed by HPLC–PDA–ESI–MS/MS analysis. The phytochemical analysis identified 39 compounds, the majority of them were flavan-3-ol derivatives and condensed tannins. Total phenolic content, determined by the Folin–Ciocalteu method amounted to 489 mg gallic acid equivalents/g extract. The extract showed promising antioxidant activities with an EC
50
of 3.6 µg/mL and 18.32 mM FeSO
4
equivalent/mg extract in radical scavenging assay and ferric reducing antioxidant power assays, respectively. The hepatoprotective potential of the extract in rats was determined in vivo in a
d-
galactosamine-induced liver toxicity model. A dose of 100 mg/kg (body weight) of the bark extract reduced levels of aspartate aminotransferase, gamma-glutamyltransferase and total bilirubin by 35.7, 65.3, and 23.8% (
p
< 0.05), respectively whereas glutathione was increased by 59.1%. These effects were similar to silymarin which was used as positive control. The extract (100 mg/kg (body weight) mediated a substantial antidiabetic response in streptozotocin-induced diabetic rats manifested by a significant reduction in serum glucose and lipid peroxides and significant increase of serum insulin. Docking of
d
-(+) catechin and the dimer (epi)catechin-(epi)catechin into the active site of the enzymes human pancreatic α-amylase, maltase-glucoamylase, and aldol reductase revealed that these enzymes may be possible targets via which, the studied
Albizia harveyi
extract could exert its antidiabetic effect.</description><identifier>ISSN: 1054-2523</identifier><identifier>EISSN: 1554-8120</identifier><identifier>DOI: 10.1007/s00044-017-2005-8</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Aldehydes ; Antioxidants ; Aspartate aminotransferase ; Bark ; Bilirubin ; Biochemistry ; Biocompatibility ; Biomedical and Life Sciences ; Biomedicine ; Body weight ; Catechin ; Cell Biology ; D-Galactosamine ; Diabetes mellitus ; Docking ; Enzymes ; Equivalence ; Gallic acid ; Glucoamylase ; Glutathione ; High-performance liquid chromatography ; In vivo methods and tests ; Insulin ; Liver ; Original Research ; Pancreas ; Peroxides ; Pharmacology/Toxicology ; Phenolic compounds ; Phytochemicals ; Plant extracts ; Polyphenols ; Rats ; Reductase ; Rodents ; Silymarin ; Streptozocin ; Tannins ; Toxicity ; Weight reduction ; α-Amylase ; γ-Glutamyltransferase</subject><ispartof>Medicinal chemistry research, 2017-12, Vol.26 (12), p.3091-3105</ispartof><rights>Springer Science+Business Media, LLC 2017</rights><rights>Copyright Springer Science & Business Media 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-624448b47a7451f8717bf1692fb5259e896359f8347e2e600f027d027b2135d43</citedby><cites>FETCH-LOGICAL-c316t-624448b47a7451f8717bf1692fb5259e896359f8347e2e600f027d027b2135d43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00044-017-2005-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00044-017-2005-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Sobeh, Mansour</creatorcontrib><creatorcontrib>Mahmoud, Mona F.</creatorcontrib><creatorcontrib>Abdelfattah, Mohamed A. O.</creatorcontrib><creatorcontrib>El-Beshbishy, Hesham A.</creatorcontrib><creatorcontrib>El-Shazly, Assem M.</creatorcontrib><creatorcontrib>Wink, Michael</creatorcontrib><title>Albizia harveyi: phytochemical profiling, antioxidant, antidiabetic and hepatoprotective activities of the bark extract</title><title>Medicinal chemistry research</title><addtitle>Med Chem Res</addtitle><description>Profiling the polyphenols in the methanol extract from the bark of
Albizia harveyi
was performed by HPLC–PDA–ESI–MS/MS analysis. The phytochemical analysis identified 39 compounds, the majority of them were flavan-3-ol derivatives and condensed tannins. Total phenolic content, determined by the Folin–Ciocalteu method amounted to 489 mg gallic acid equivalents/g extract. The extract showed promising antioxidant activities with an EC
50
of 3.6 µg/mL and 18.32 mM FeSO
4
equivalent/mg extract in radical scavenging assay and ferric reducing antioxidant power assays, respectively. The hepatoprotective potential of the extract in rats was determined in vivo in a
d-
galactosamine-induced liver toxicity model. A dose of 100 mg/kg (body weight) of the bark extract reduced levels of aspartate aminotransferase, gamma-glutamyltransferase and total bilirubin by 35.7, 65.3, and 23.8% (
p
< 0.05), respectively whereas glutathione was increased by 59.1%. These effects were similar to silymarin which was used as positive control. The extract (100 mg/kg (body weight) mediated a substantial antidiabetic response in streptozotocin-induced diabetic rats manifested by a significant reduction in serum glucose and lipid peroxides and significant increase of serum insulin. Docking of
d
-(+) catechin and the dimer (epi)catechin-(epi)catechin into the active site of the enzymes human pancreatic α-amylase, maltase-glucoamylase, and aldol reductase revealed that these enzymes may be possible targets via which, the studied
Albizia harveyi
extract could exert its antidiabetic effect.</description><subject>Aldehydes</subject><subject>Antioxidants</subject><subject>Aspartate aminotransferase</subject><subject>Bark</subject><subject>Bilirubin</subject><subject>Biochemistry</subject><subject>Biocompatibility</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Body weight</subject><subject>Catechin</subject><subject>Cell Biology</subject><subject>D-Galactosamine</subject><subject>Diabetes mellitus</subject><subject>Docking</subject><subject>Enzymes</subject><subject>Equivalence</subject><subject>Gallic acid</subject><subject>Glucoamylase</subject><subject>Glutathione</subject><subject>High-performance liquid chromatography</subject><subject>In vivo methods and tests</subject><subject>Insulin</subject><subject>Liver</subject><subject>Original Research</subject><subject>Pancreas</subject><subject>Peroxides</subject><subject>Pharmacology/Toxicology</subject><subject>Phenolic compounds</subject><subject>Phytochemicals</subject><subject>Plant extracts</subject><subject>Polyphenols</subject><subject>Rats</subject><subject>Reductase</subject><subject>Rodents</subject><subject>Silymarin</subject><subject>Streptozocin</subject><subject>Tannins</subject><subject>Toxicity</subject><subject>Weight reduction</subject><subject>α-Amylase</subject><subject>γ-Glutamyltransferase</subject><issn>1054-2523</issn><issn>1554-8120</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNp1UMtOwzAQtBBIlMIHcLPElcDaseOEW1XxkpC4wNlyErtxSZNgu6Xl63EUDlw4rGZXOzO7GoQuCdwQAHHrAYCxBIhIKABP8iM0I5yzJCcUjmMPsaecpqfozPs1QCqA8Rn6WrSl_bYKN8rt9MHe4aE5hL5q9MZWqsWD641tbbe6xqoLtt_bOuI01FaVOtgqDjVu9KBCH-lBV8HuNFYj2GC1x73BodG4VO4D631wcXWOToxqvb74xTl6f7h_Wz4lL6-Pz8vFS1KlJAtJRhljecmEEowTkwsiSkOygpqSU17ovMhSXpg8ZUJTnQEYoKKOVVKS8pqlc3Q1-cbPPrfaB7nut66LJyUpMsJEymgeWWRiVa733mkjB2c3yh0kATnmK6d8ZcxXjvnKUUMnjY_cbqXdH-d_RT9gCX4N</recordid><startdate>20171201</startdate><enddate>20171201</enddate><creator>Sobeh, Mansour</creator><creator>Mahmoud, Mona F.</creator><creator>Abdelfattah, Mohamed A. O.</creator><creator>El-Beshbishy, Hesham A.</creator><creator>El-Shazly, Assem M.</creator><creator>Wink, Michael</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope></search><sort><creationdate>20171201</creationdate><title>Albizia harveyi: phytochemical profiling, antioxidant, antidiabetic and hepatoprotective activities of the bark extract</title><author>Sobeh, Mansour ; Mahmoud, Mona F. ; Abdelfattah, Mohamed A. O. ; El-Beshbishy, Hesham A. ; El-Shazly, Assem M. ; Wink, Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-624448b47a7451f8717bf1692fb5259e896359f8347e2e600f027d027b2135d43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Aldehydes</topic><topic>Antioxidants</topic><topic>Aspartate aminotransferase</topic><topic>Bark</topic><topic>Bilirubin</topic><topic>Biochemistry</topic><topic>Biocompatibility</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Body weight</topic><topic>Catechin</topic><topic>Cell Biology</topic><topic>D-Galactosamine</topic><topic>Diabetes mellitus</topic><topic>Docking</topic><topic>Enzymes</topic><topic>Equivalence</topic><topic>Gallic acid</topic><topic>Glucoamylase</topic><topic>Glutathione</topic><topic>High-performance liquid chromatography</topic><topic>In vivo methods and tests</topic><topic>Insulin</topic><topic>Liver</topic><topic>Original Research</topic><topic>Pancreas</topic><topic>Peroxides</topic><topic>Pharmacology/Toxicology</topic><topic>Phenolic compounds</topic><topic>Phytochemicals</topic><topic>Plant extracts</topic><topic>Polyphenols</topic><topic>Rats</topic><topic>Reductase</topic><topic>Rodents</topic><topic>Silymarin</topic><topic>Streptozocin</topic><topic>Tannins</topic><topic>Toxicity</topic><topic>Weight reduction</topic><topic>α-Amylase</topic><topic>γ-Glutamyltransferase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sobeh, Mansour</creatorcontrib><creatorcontrib>Mahmoud, Mona F.</creatorcontrib><creatorcontrib>Abdelfattah, Mohamed A. O.</creatorcontrib><creatorcontrib>El-Beshbishy, Hesham A.</creatorcontrib><creatorcontrib>El-Shazly, Assem M.</creatorcontrib><creatorcontrib>Wink, Michael</creatorcontrib><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Medicinal chemistry research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sobeh, Mansour</au><au>Mahmoud, Mona F.</au><au>Abdelfattah, Mohamed A. O.</au><au>El-Beshbishy, Hesham A.</au><au>El-Shazly, Assem M.</au><au>Wink, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Albizia harveyi: phytochemical profiling, antioxidant, antidiabetic and hepatoprotective activities of the bark extract</atitle><jtitle>Medicinal chemistry research</jtitle><stitle>Med Chem Res</stitle><date>2017-12-01</date><risdate>2017</risdate><volume>26</volume><issue>12</issue><spage>3091</spage><epage>3105</epage><pages>3091-3105</pages><issn>1054-2523</issn><eissn>1554-8120</eissn><abstract>Profiling the polyphenols in the methanol extract from the bark of
Albizia harveyi
was performed by HPLC–PDA–ESI–MS/MS analysis. The phytochemical analysis identified 39 compounds, the majority of them were flavan-3-ol derivatives and condensed tannins. Total phenolic content, determined by the Folin–Ciocalteu method amounted to 489 mg gallic acid equivalents/g extract. The extract showed promising antioxidant activities with an EC
50
of 3.6 µg/mL and 18.32 mM FeSO
4
equivalent/mg extract in radical scavenging assay and ferric reducing antioxidant power assays, respectively. The hepatoprotective potential of the extract in rats was determined in vivo in a
d-
galactosamine-induced liver toxicity model. A dose of 100 mg/kg (body weight) of the bark extract reduced levels of aspartate aminotransferase, gamma-glutamyltransferase and total bilirubin by 35.7, 65.3, and 23.8% (
p
< 0.05), respectively whereas glutathione was increased by 59.1%. These effects were similar to silymarin which was used as positive control. The extract (100 mg/kg (body weight) mediated a substantial antidiabetic response in streptozotocin-induced diabetic rats manifested by a significant reduction in serum glucose and lipid peroxides and significant increase of serum insulin. Docking of
d
-(+) catechin and the dimer (epi)catechin-(epi)catechin into the active site of the enzymes human pancreatic α-amylase, maltase-glucoamylase, and aldol reductase revealed that these enzymes may be possible targets via which, the studied
Albizia harveyi
extract could exert its antidiabetic effect.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s00044-017-2005-8</doi><tpages>15</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1054-2523 |
| ispartof | Medicinal chemistry research, 2017-12, Vol.26 (12), p.3091-3105 |
| issn | 1054-2523 1554-8120 |
| language | eng |
| recordid | cdi_proquest_journals_1961473428 |
| source | SpringerNature Journals |
| subjects | Aldehydes Antioxidants Aspartate aminotransferase Bark Bilirubin Biochemistry Biocompatibility Biomedical and Life Sciences Biomedicine Body weight Catechin Cell Biology D-Galactosamine Diabetes mellitus Docking Enzymes Equivalence Gallic acid Glucoamylase Glutathione High-performance liquid chromatography In vivo methods and tests Insulin Liver Original Research Pancreas Peroxides Pharmacology/Toxicology Phenolic compounds Phytochemicals Plant extracts Polyphenols Rats Reductase Rodents Silymarin Streptozocin Tannins Toxicity Weight reduction α-Amylase γ-Glutamyltransferase |
| title | Albizia harveyi: phytochemical profiling, antioxidant, antidiabetic and hepatoprotective activities of the bark extract |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T03%3A49%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Albizia%20harveyi:%20phytochemical%20profiling,%20antioxidant,%20antidiabetic%20and%20hepatoprotective%20activities%20of%20the%20bark%20extract&rft.jtitle=Medicinal%20chemistry%20research&rft.au=Sobeh,%20Mansour&rft.date=2017-12-01&rft.volume=26&rft.issue=12&rft.spage=3091&rft.epage=3105&rft.pages=3091-3105&rft.issn=1054-2523&rft.eissn=1554-8120&rft_id=info:doi/10.1007/s00044-017-2005-8&rft_dat=%3Cproquest_cross%3E1961473428%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1961473428&rft_id=info:pmid/&rfr_iscdi=true |