Colorimetric enzyme-coupled assay for hyaluronic acid determination in complex samples

[Display omitted] •A highly sensitive colorimetric assay for hyaluronic acid determination is proposed.•Assay is based on 3-methyl-2-benothiazolinonehydrazone reaction with reducing ends.•S. pneumoniae lyase is used for hyaluronic acid depolymerization to disaccharides.•The sensitivity of new assay...

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Veröffentlicht in:	European polymer journal 2017-09, Vol.94, p.460-470
Hauptverfasser:	Pepeliaev, Stanislav, Hrudíková, Radka, Jílková, Jana, Pavlík, Jaroslav, Smirnou, Dzianis, Černý, Zbyněk, Franke, Lukáš
Format:	Artikel
Sprache:	eng
Schlagworte:	Acids Analytical chemistry Cetyltrimethylammonium bromide Colorimetry Complexity Glycosaminoglycan precipitation Glycosaminoglycans Hyaluronic acid Hyaluronic acid assay Oligosaccharides Sensitivity Streptococcus pneumoniae hyaluronan lyase Veterinary medicine Viscosity
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