Study of the effect of the operating parameters on the separation of bioactive compounds of palm oil by ultra‐high performance supercritical fluid chromatography using a design of experiments approach
In this study, an analytical method based on Design of Experiments and response surface methodology for the separation of lycopene, beta‐carotene, coenzyme Q10, and lutein was developed by ultra‐high performance supercritical fluid chromatography technique. A Central Composite Design was used to eva...
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Veröffentlicht in: | Canadian journal of chemical engineering 2017-12, Vol.95 (12), p.2306-2314 |
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description | In this study, an analytical method based on Design of Experiments and response surface methodology for the separation of lycopene, beta‐carotene, coenzyme Q10, and lutein was developed by ultra‐high performance supercritical fluid chromatography technique. A Central Composite Design was used to evaluate the influence of temperature, pressure, and ethanol percentage on the retention and separation factors of these compounds. In the designed experiments, the temperature was varied between 25 and 50 °C (298 and 323 K), the pressure within the interval of 1500 and 2200 psi (10 and 15 MPa), and the ethanol percentage between 15 and 24 (v/v) %. Each variable was tested at 3 levels and 5 replicated central points were added. It was found for the studied system that the ethanol percentage and pressure were the parameters that most influenced the retention factors whereas the ethanol percentage, pressure, and temperature affected the separation factors. Quadratic models were necessary to describe the retention and separation of these compounds. Furthermore, interactions among the factors were observed, justifying the DOE approach used in this work. Considering the retention and separation factors, the selected operating conditions for further experiments were: 15.5 % of ethanol at 40 °C (313 K) and 1500 psi (10 MPa). |
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A Central Composite Design was used to evaluate the influence of temperature, pressure, and ethanol percentage on the retention and separation factors of these compounds. In the designed experiments, the temperature was varied between 25 and 50 °C (298 and 323 K), the pressure within the interval of 1500 and 2200 psi (10 and 15 MPa), and the ethanol percentage between 15 and 24 (v/v) %. Each variable was tested at 3 levels and 5 replicated central points were added. It was found for the studied system that the ethanol percentage and pressure were the parameters that most influenced the retention factors whereas the ethanol percentage, pressure, and temperature affected the separation factors. Quadratic models were necessary to describe the retention and separation of these compounds. Furthermore, interactions among the factors were observed, justifying the DOE approach used in this work. Considering the retention and separation factors, the selected operating conditions for further experiments were: 15.5 % of ethanol at 40 °C (313 K) and 1500 psi (10 MPa).</description><identifier>ISSN: 0008-4034</identifier><identifier>EISSN: 1939-019X</identifier><identifier>DOI: 10.1002/cjce.22969</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc</publisher><subject>Carotene ; carotenoids ; Chromatography ; coenzyme Q10 ; Design of experiments ; Ethanol ; Lutein ; Palm oil ; Response surface methodology ; Retention ; Separation ; separation process ; ultra‐high performance supercritical fluid chromatography</subject><ispartof>Canadian journal of chemical engineering, 2017-12, Vol.95 (12), p.2306-2314</ispartof><rights>2017 Canadian Society for Chemical Engineering</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3389-d0b589e43ff478ea45f4c4c5dfaf21bcacf7926b645bb7becbe10af6471f57d53</citedby><cites>FETCH-LOGICAL-c3389-d0b589e43ff478ea45f4c4c5dfaf21bcacf7926b645bb7becbe10af6471f57d53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcjce.22969$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcjce.22969$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids></links><search><creatorcontrib>de Souza Guedes, Luciana</creatorcontrib><creatorcontrib>Jardim, Isabel Cristina Sales Fontes</creatorcontrib><creatorcontrib>de Melo, Lucília Vilela</creatorcontrib><creatorcontrib>Beppu, Marisa Massumi</creatorcontrib><creatorcontrib>Breitkreitz, Márcia Cristina</creatorcontrib><creatorcontrib>Santana, Cesar Costapinto</creatorcontrib><title>Study of the effect of the operating parameters on the separation of bioactive compounds of palm oil by ultra‐high performance supercritical fluid chromatography using a design of experiments approach</title><title>Canadian journal of chemical engineering</title><description>In this study, an analytical method based on Design of Experiments and response surface methodology for the separation of lycopene, beta‐carotene, coenzyme Q10, and lutein was developed by ultra‐high performance supercritical fluid chromatography technique. A Central Composite Design was used to evaluate the influence of temperature, pressure, and ethanol percentage on the retention and separation factors of these compounds. In the designed experiments, the temperature was varied between 25 and 50 °C (298 and 323 K), the pressure within the interval of 1500 and 2200 psi (10 and 15 MPa), and the ethanol percentage between 15 and 24 (v/v) %. Each variable was tested at 3 levels and 5 replicated central points were added. It was found for the studied system that the ethanol percentage and pressure were the parameters that most influenced the retention factors whereas the ethanol percentage, pressure, and temperature affected the separation factors. Quadratic models were necessary to describe the retention and separation of these compounds. Furthermore, interactions among the factors were observed, justifying the DOE approach used in this work. Considering the retention and separation factors, the selected operating conditions for further experiments were: 15.5 % of ethanol at 40 °C (313 K) and 1500 psi (10 MPa).</description><subject>Carotene</subject><subject>carotenoids</subject><subject>Chromatography</subject><subject>coenzyme Q10</subject><subject>Design of experiments</subject><subject>Ethanol</subject><subject>Lutein</subject><subject>Palm oil</subject><subject>Response surface methodology</subject><subject>Retention</subject><subject>Separation</subject><subject>separation process</subject><subject>ultra‐high performance supercritical fluid chromatography</subject><issn>0008-4034</issn><issn>1939-019X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNp9kc1u1DAUhS1EJYbSTZ_AErtKKXbi_HiJRuWnqsSCIrGLrp3riUdJnNoOdHY8Qp-Lx-iT4MzAlpV1rr9zz5UOIZecXXPG8nd6r_E6z2UlX5ANl4XMGJffX5INY6zJBCvEK_I6hH2SORN8Q35_jUt3oM7Q2CNFY1DHf8rN6CHaaUdn8DBiRB-om45_AddZtEkmWlkHOtofSLUbZ7dMXVjHMwwjdXag6kCXIXp4_vXU211P02Lj_AiTTpuWpLS30WoYqBkW21HdezdCdDsPc5-8YT0CaIfB7o6B-JhMdsQpBgrz7FN8_4acGRgCXvx9z8m3Dzf320_Z3ZePn7fv7zJdFI3MOqbKRqIojBF1gyBKI7TQZWfA5Fxp0KaWeaUqUSpVK9QKOQNTiZqbsu7K4py8Pe1NsQ8Lhtju3eKnFNlyWXFWVA2rE3V1orR3IXg07ZwOBn9oOWvXrtq1q_bYVYL5Cf5pBzz8h2y3t9ubk-cPzAyetg</recordid><startdate>201712</startdate><enddate>201712</enddate><creator>de Souza Guedes, Luciana</creator><creator>Jardim, Isabel Cristina Sales Fontes</creator><creator>de Melo, Lucília Vilela</creator><creator>Beppu, Marisa Massumi</creator><creator>Breitkreitz, Márcia Cristina</creator><creator>Santana, Cesar Costapinto</creator><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>201712</creationdate><title>Study of the effect of the operating parameters on the separation of bioactive compounds of palm oil by ultra‐high performance supercritical fluid chromatography using a design of experiments approach</title><author>de Souza Guedes, Luciana ; Jardim, Isabel Cristina Sales Fontes ; de Melo, Lucília Vilela ; Beppu, Marisa Massumi ; Breitkreitz, Márcia Cristina ; Santana, Cesar Costapinto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3389-d0b589e43ff478ea45f4c4c5dfaf21bcacf7926b645bb7becbe10af6471f57d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Carotene</topic><topic>carotenoids</topic><topic>Chromatography</topic><topic>coenzyme Q10</topic><topic>Design of experiments</topic><topic>Ethanol</topic><topic>Lutein</topic><topic>Palm oil</topic><topic>Response surface methodology</topic><topic>Retention</topic><topic>Separation</topic><topic>separation process</topic><topic>ultra‐high performance supercritical fluid chromatography</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Souza Guedes, Luciana</creatorcontrib><creatorcontrib>Jardim, Isabel Cristina Sales Fontes</creatorcontrib><creatorcontrib>de Melo, Lucília Vilela</creatorcontrib><creatorcontrib>Beppu, Marisa Massumi</creatorcontrib><creatorcontrib>Breitkreitz, Márcia Cristina</creatorcontrib><creatorcontrib>Santana, Cesar Costapinto</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Canadian journal of chemical engineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Souza Guedes, Luciana</au><au>Jardim, Isabel Cristina Sales Fontes</au><au>de Melo, Lucília Vilela</au><au>Beppu, Marisa Massumi</au><au>Breitkreitz, Márcia Cristina</au><au>Santana, Cesar Costapinto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Study of the effect of the operating parameters on the separation of bioactive compounds of palm oil by ultra‐high performance supercritical fluid chromatography using a design of experiments approach</atitle><jtitle>Canadian journal of chemical engineering</jtitle><date>2017-12</date><risdate>2017</risdate><volume>95</volume><issue>12</issue><spage>2306</spage><epage>2314</epage><pages>2306-2314</pages><issn>0008-4034</issn><eissn>1939-019X</eissn><abstract>In this study, an analytical method based on Design of Experiments and response surface methodology for the separation of lycopene, beta‐carotene, coenzyme Q10, and lutein was developed by ultra‐high performance supercritical fluid chromatography technique. A Central Composite Design was used to evaluate the influence of temperature, pressure, and ethanol percentage on the retention and separation factors of these compounds. In the designed experiments, the temperature was varied between 25 and 50 °C (298 and 323 K), the pressure within the interval of 1500 and 2200 psi (10 and 15 MPa), and the ethanol percentage between 15 and 24 (v/v) %. Each variable was tested at 3 levels and 5 replicated central points were added. It was found for the studied system that the ethanol percentage and pressure were the parameters that most influenced the retention factors whereas the ethanol percentage, pressure, and temperature affected the separation factors. Quadratic models were necessary to describe the retention and separation of these compounds. Furthermore, interactions among the factors were observed, justifying the DOE approach used in this work. Considering the retention and separation factors, the selected operating conditions for further experiments were: 15.5 % of ethanol at 40 °C (313 K) and 1500 psi (10 MPa).</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1002/cjce.22969</doi><tpages>9</tpages></addata></record> |
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subjects | Carotene carotenoids Chromatography coenzyme Q10 Design of experiments Ethanol Lutein Palm oil Response surface methodology Retention Separation separation process ultra‐high performance supercritical fluid chromatography |
title | Study of the effect of the operating parameters on the separation of bioactive compounds of palm oil by ultra‐high performance supercritical fluid chromatography using a design of experiments approach |
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