MicroRNA-145 inhibits tumorigenesis and invasion of cervical cancer stem cells
MicroRNA (miR)-145 has been reported to induce cancer stem cell (CSC) differentiation through down-regulation of the stem cell transcription factors (TFs) that maintain CSC pluripotency. High expression of miR-145 indicates a good prognosis in cancer patients, but its role in cervical cancer stem ce...
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| Veröffentlicht in: | International journal of oncology 2017-03, Vol.50 (3), p.853-862 |
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| creator | Zhou, Xi Yue, Yan Wang, Renxiao Gong, Baolan Duan, Zhao |
| description | MicroRNA (miR)-145 has been reported to induce cancer stem cell (CSC) differentiation through down-regulation of the stem cell transcription factors (TFs) that maintain CSC pluripotency. High expression of miR-145 indicates a good prognosis in cancer patients, but its role in cervical cancer stem cells (CCSCs) is not known. We show that expression of miR-145 and core stem cell transcription factors, Sox2, Nanog and Oct4, are associated with the pluripotency of CCSCs, with increased expression of miR-145 after cervical tumorsphere (CT) differentiation. miR-145 overexpression inhibited expression of core TFs, as well as decreasing tumor invasion and colony formation, whereas miR-145 knockdown led to the opposite effects. Injection of adenovirus-miR-145 significantly reduced tumor growth in nude mice. High miR-145 expression predicted a better prognosis compared with that in patients with low miR-145 expression after analyses of The Cancer Genome Atlas (TCGA) data. These results suggest that miR-145 is able to induce CT differentiation through enzymolyzing TFs and might be a therapeutic target for cervical carcinoma. |
| doi_str_mv | 10.3892/ijo.2017.3857 |
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High expression of miR-145 indicates a good prognosis in cancer patients, but its role in cervical cancer stem cells (CCSCs) is not known. We show that expression of miR-145 and core stem cell transcription factors, Sox2, Nanog and Oct4, are associated with the pluripotency of CCSCs, with increased expression of miR-145 after cervical tumorsphere (CT) differentiation. miR-145 overexpression inhibited expression of core TFs, as well as decreasing tumor invasion and colony formation, whereas miR-145 knockdown led to the opposite effects. Injection of adenovirus-miR-145 significantly reduced tumor growth in nude mice. High miR-145 expression predicted a better prognosis compared with that in patients with low miR-145 expression after analyses of The Cancer Genome Atlas (TCGA) data. These results suggest that miR-145 is able to induce CT differentiation through enzymolyzing TFs and might be a therapeutic target for cervical carcinoma.</description><identifier>ISSN: 1019-6439</identifier><identifier>EISSN: 1791-2423</identifier><identifier>DOI: 10.3892/ijo.2017.3857</identifier><identifier>PMID: 28112371</identifier><language>eng</language><publisher>Greece: Spandidos Publications</publisher><subject>Adenoviruses ; Adult ; Aged ; Animals ; Cancer therapies ; Carcinogenesis - genetics ; Care and treatment ; Cell Proliferation - genetics ; Cervical cancer ; Cloning ; Development and progression ; Female ; Gene expression ; Gene Knockdown Techniques ; Genetic aspects ; Health aspects ; Humans ; Medical prognosis ; Metastasis ; Mice ; Mice, Nude ; MicroRNA ; MicroRNAs - genetics ; Middle Aged ; Nanog Homeobox Protein - metabolism ; Neoplasm Invasiveness - genetics ; Neoplastic Stem Cells - pathology ; Octamer Transcription Factor-3 - metabolism ; Pluripotent Stem Cells - pathology ; Prognosis ; SOXB1 Transcription Factors - metabolism ; Stem cells ; Transcription factors ; Tumor Cells, Cultured ; Tumors ; Uterine Cervical Neoplasms - genetics ; Uterine Cervical Neoplasms - pathology</subject><ispartof>International journal of oncology, 2017-03, Vol.50 (3), p.853-862</ispartof><rights>COPYRIGHT 2017 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2017</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c524t-949fb1057a86d964c400d5baa6749e3cbff03541fbc3e154a8352cae45d54e843</citedby><cites>FETCH-LOGICAL-c524t-949fb1057a86d964c400d5baa6749e3cbff03541fbc3e154a8352cae45d54e843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28112371$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhou, Xi</creatorcontrib><creatorcontrib>Yue, Yan</creatorcontrib><creatorcontrib>Wang, Renxiao</creatorcontrib><creatorcontrib>Gong, Baolan</creatorcontrib><creatorcontrib>Duan, Zhao</creatorcontrib><title>MicroRNA-145 inhibits tumorigenesis and invasion of cervical cancer stem cells</title><title>International journal of oncology</title><addtitle>Int J Oncol</addtitle><description>MicroRNA (miR)-145 has been reported to induce cancer stem cell (CSC) differentiation through down-regulation of the stem cell transcription factors (TFs) that maintain CSC pluripotency. High expression of miR-145 indicates a good prognosis in cancer patients, but its role in cervical cancer stem cells (CCSCs) is not known. We show that expression of miR-145 and core stem cell transcription factors, Sox2, Nanog and Oct4, are associated with the pluripotency of CCSCs, with increased expression of miR-145 after cervical tumorsphere (CT) differentiation. miR-145 overexpression inhibited expression of core TFs, as well as decreasing tumor invasion and colony formation, whereas miR-145 knockdown led to the opposite effects. Injection of adenovirus-miR-145 significantly reduced tumor growth in nude mice. High miR-145 expression predicted a better prognosis compared with that in patients with low miR-145 expression after analyses of The Cancer Genome Atlas (TCGA) data. These results suggest that miR-145 is able to induce CT differentiation through enzymolyzing TFs and might be a therapeutic target for cervical carcinoma.</description><subject>Adenoviruses</subject><subject>Adult</subject><subject>Aged</subject><subject>Animals</subject><subject>Cancer therapies</subject><subject>Carcinogenesis - genetics</subject><subject>Care and treatment</subject><subject>Cell Proliferation - genetics</subject><subject>Cervical cancer</subject><subject>Cloning</subject><subject>Development and progression</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Knockdown Techniques</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Medical prognosis</subject><subject>Metastasis</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>MicroRNA</subject><subject>MicroRNAs - genetics</subject><subject>Middle Aged</subject><subject>Nanog Homeobox Protein - metabolism</subject><subject>Neoplasm Invasiveness - genetics</subject><subject>Neoplastic Stem Cells - pathology</subject><subject>Octamer Transcription Factor-3 - metabolism</subject><subject>Pluripotent Stem Cells - pathology</subject><subject>Prognosis</subject><subject>SOXB1 Transcription Factors - metabolism</subject><subject>Stem cells</subject><subject>Transcription factors</subject><subject>Tumor Cells, Cultured</subject><subject>Tumors</subject><subject>Uterine Cervical Neoplasms - genetics</subject><subject>Uterine Cervical Neoplasms - pathology</subject><issn>1019-6439</issn><issn>1791-2423</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNptkUtLxDAQgIMouq4evUpB8NY1k0fbHJfFF_gA0XNI08TN0jZr0gr-e7P4Bskhk8k3EzIfQkeAZ7QS5Myt_IxgKNOJl1toAqWAnDBCt1OMQeQFo2IP7ce4wphwjmEX7ZEKgNASJuju1ungH-7mOTCeuX7pajfEbBg7H9yz6U10MVN9k65eVXS-z7zNtAmvTqs206pPcRYH06Vk28YDtGNVG83h5z5FTxfnj4ur_Ob-8noxv8k1J2zIBRO2BsxLVRWNKJhmGDe8VqoomTBU19ZiyhnYWlMDnKmKcqKVYbzhzFSMTtHJR9918C-jiYNc-TH06UkJgpcFE0TQH-pZtUa63vohKN25qOWcCQBe4gonavYPlVZjOqd9b6xL-T8Fp78Klka1wzL6dhzSeOJfMP8A04hjDMbKdXCdCm8SsNzYk8me3NiTG3uJP_781Vh3pvmmv3TRd1h3keg</recordid><startdate>20170301</startdate><enddate>20170301</enddate><creator>Zhou, Xi</creator><creator>Yue, Yan</creator><creator>Wang, Renxiao</creator><creator>Gong, Baolan</creator><creator>Duan, Zhao</creator><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20170301</creationdate><title>MicroRNA-145 inhibits tumorigenesis and invasion of cervical cancer stem cells</title><author>Zhou, Xi ; 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High expression of miR-145 indicates a good prognosis in cancer patients, but its role in cervical cancer stem cells (CCSCs) is not known. We show that expression of miR-145 and core stem cell transcription factors, Sox2, Nanog and Oct4, are associated with the pluripotency of CCSCs, with increased expression of miR-145 after cervical tumorsphere (CT) differentiation. miR-145 overexpression inhibited expression of core TFs, as well as decreasing tumor invasion and colony formation, whereas miR-145 knockdown led to the opposite effects. Injection of adenovirus-miR-145 significantly reduced tumor growth in nude mice. High miR-145 expression predicted a better prognosis compared with that in patients with low miR-145 expression after analyses of The Cancer Genome Atlas (TCGA) data. These results suggest that miR-145 is able to induce CT differentiation through enzymolyzing TFs and might be a therapeutic target for cervical carcinoma.</abstract><cop>Greece</cop><pub>Spandidos Publications</pub><pmid>28112371</pmid><doi>10.3892/ijo.2017.3857</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | Spandidos Publications Journals; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Adenoviruses Adult Aged Animals Cancer therapies Carcinogenesis - genetics Care and treatment Cell Proliferation - genetics Cervical cancer Cloning Development and progression Female Gene expression Gene Knockdown Techniques Genetic aspects Health aspects Humans Medical prognosis Metastasis Mice Mice, Nude MicroRNA MicroRNAs - genetics Middle Aged Nanog Homeobox Protein - metabolism Neoplasm Invasiveness - genetics Neoplastic Stem Cells - pathology Octamer Transcription Factor-3 - metabolism Pluripotent Stem Cells - pathology Prognosis SOXB1 Transcription Factors - metabolism Stem cells Transcription factors Tumor Cells, Cultured Tumors Uterine Cervical Neoplasms - genetics Uterine Cervical Neoplasms - pathology |
| title | MicroRNA-145 inhibits tumorigenesis and invasion of cervical cancer stem cells |
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