Enhanced Tolerance to Water Deficit and Salinity Stress in Transgenic Lycium barbarum L. Plants Ectopically Expressing ATHK1, an Arabidopsis thaliana Histidine Kinase Gene [Erratum: 2010 June, v. 28, no. 2, p. 363]
ATHK1 has been implicated in drought and salt tolerance in Arabidopsis thaliana. In this study, the full-length coding sequence of ATHK1 was introduced into Lycium barbarum L. by Agrobacterium transformation. Our results indicated that the transgenic plants tolerated high concentrations of NaCl or w...
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| Veröffentlicht in: | Plant molecular biology reporter 2009-09, Vol.27 (3), p.321-333 |
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| description | ATHK1 has been implicated in drought and salt tolerance in Arabidopsis thaliana. In this study, the full-length coding sequence of ATHK1 was introduced into Lycium barbarum L. by Agrobacterium transformation. Our results indicated that the transgenic plants tolerated high concentrations of NaCl or water deprivation and exhibited faster recovery following re-watering compared to wild type plants. Salt- or water-stressed transgenic plants had higher relative water content, proline and soluble protein levels, and lower chlorophyll losses and membrane ion leakage. In addition, they showed higher capacity for antioxidative reactions reflected by reduced hydrogen peroxide (H₂O₂), superoxide anion radical (O₂ ⁻), and lipid peroxide production and increased superoxide dismutase, catalase, and peroxidase activities. The ATHK1 transcript, as shown by reverse transcription polymerase chain reaction, was more abundant under high than low osmolarity in transgenic plants. ATHK1 therefore improved tolerance of L. barbarum to drought and salt stress. |
| doi_str_mv | 10.1007/s11105-008-0084-x |
| format | Article |
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Plants Ectopically Expressing ATHK1, an Arabidopsis thaliana Histidine Kinase Gene [Erratum: 2010 June, v. 28, no. 2, p. 363]</title><source>SpringerNature Journals</source><creator>Chen, Ni ; Liu, Yan ; Liu, Xin ; Chai, Juan ; Hu, Zhong ; Liu, Heng</creator><creatorcontrib>Chen, Ni ; Liu, Yan ; Liu, Xin ; Chai, Juan ; Hu, Zhong ; Liu, Heng</creatorcontrib><description>ATHK1 has been implicated in drought and salt tolerance in Arabidopsis thaliana. In this study, the full-length coding sequence of ATHK1 was introduced into Lycium barbarum L. by Agrobacterium transformation. Our results indicated that the transgenic plants tolerated high concentrations of NaCl or water deprivation and exhibited faster recovery following re-watering compared to wild type plants. Salt- or water-stressed transgenic plants had higher relative water content, proline and soluble protein levels, and lower chlorophyll losses and membrane ion leakage. In addition, they showed higher capacity for antioxidative reactions reflected by reduced hydrogen peroxide (H₂O₂), superoxide anion radical (O₂ ⁻), and lipid peroxide production and increased superoxide dismutase, catalase, and peroxidase activities. The ATHK1 transcript, as shown by reverse transcription polymerase chain reaction, was more abundant under high than low osmolarity in transgenic plants. ATHK1 therefore improved tolerance of L. barbarum to drought and salt stress.</description><identifier>ISSN: 0735-9640</identifier><identifier>EISSN: 1572-9818</identifier><identifier>DOI: 10.1007/s11105-008-0084-x</identifier><language>eng</language><publisher>New York: New York : Springer-Verlag</publisher><subject>Agrobacterium ; Arabidopsis thaliana ; Bioinformatics ; Biomedical and Life Sciences ; Catalase ; Chlorophyll ; Drought ; drought tolerance ; enzyme activity ; genetic transformation ; Histidine ; Histidine kinase ; Hydrogen peroxide ; Kinases ; Life Sciences ; Lycium barbarum ; Metabolomics ; Moisture content ; Osmolarity ; Peroxidase ; peroxidases ; Plant Breeding/Biotechnology ; Plant Sciences ; plant stress ; Polymerase chain reaction ; Proline ; Proteomics ; reverse transcriptase polymerase chain reaction ; Reverse transcription ; salinity ; salt stress ; Salt tolerance ; Sodium chloride ; superoxide anion ; Superoxide dismutase ; Transgenic plants ; Water content ; Water deficit ; Water deprivation ; water stress</subject><ispartof>Plant molecular biology reporter, 2009-09, Vol.27 (3), p.321-333</ispartof><rights>Springer-Verlag 2008</rights><rights>Plant Molecular Biology Reporter is a copyright of Springer, 2009.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c340t-2e11245421f02bd717bf5baff88ccf47c5436c1009110ffd382179226e9812293</citedby><cites>FETCH-LOGICAL-c340t-2e11245421f02bd717bf5baff88ccf47c5436c1009110ffd382179226e9812293</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11105-008-0084-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11105-008-0084-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Chen, Ni</creatorcontrib><creatorcontrib>Liu, Yan</creatorcontrib><creatorcontrib>Liu, Xin</creatorcontrib><creatorcontrib>Chai, Juan</creatorcontrib><creatorcontrib>Hu, Zhong</creatorcontrib><creatorcontrib>Liu, Heng</creatorcontrib><title>Enhanced Tolerance to Water Deficit and Salinity Stress in Transgenic Lycium barbarum L. Plants Ectopically Expressing ATHK1, an Arabidopsis thaliana Histidine Kinase Gene [Erratum: 2010 June, v. 28, no. 2, p. 363]</title><title>Plant molecular biology reporter</title><addtitle>Plant Mol Biol Rep</addtitle><description>ATHK1 has been implicated in drought and salt tolerance in Arabidopsis thaliana. In this study, the full-length coding sequence of ATHK1 was introduced into Lycium barbarum L. by Agrobacterium transformation. Our results indicated that the transgenic plants tolerated high concentrations of NaCl or water deprivation and exhibited faster recovery following re-watering compared to wild type plants. Salt- or water-stressed transgenic plants had higher relative water content, proline and soluble protein levels, and lower chlorophyll losses and membrane ion leakage. In addition, they showed higher capacity for antioxidative reactions reflected by reduced hydrogen peroxide (H₂O₂), superoxide anion radical (O₂ ⁻), and lipid peroxide production and increased superoxide dismutase, catalase, and peroxidase activities. The ATHK1 transcript, as shown by reverse transcription polymerase chain reaction, was more abundant under high than low osmolarity in transgenic plants. ATHK1 therefore improved tolerance of L. barbarum to drought and salt stress.</description><subject>Agrobacterium</subject><subject>Arabidopsis thaliana</subject><subject>Bioinformatics</subject><subject>Biomedical and Life Sciences</subject><subject>Catalase</subject><subject>Chlorophyll</subject><subject>Drought</subject><subject>drought tolerance</subject><subject>enzyme activity</subject><subject>genetic transformation</subject><subject>Histidine</subject><subject>Histidine kinase</subject><subject>Hydrogen peroxide</subject><subject>Kinases</subject><subject>Life Sciences</subject><subject>Lycium barbarum</subject><subject>Metabolomics</subject><subject>Moisture content</subject><subject>Osmolarity</subject><subject>Peroxidase</subject><subject>peroxidases</subject><subject>Plant Breeding/Biotechnology</subject><subject>Plant Sciences</subject><subject>plant stress</subject><subject>Polymerase chain reaction</subject><subject>Proline</subject><subject>Proteomics</subject><subject>reverse transcriptase polymerase chain reaction</subject><subject>Reverse transcription</subject><subject>salinity</subject><subject>salt stress</subject><subject>Salt tolerance</subject><subject>Sodium chloride</subject><subject>superoxide anion</subject><subject>Superoxide dismutase</subject><subject>Transgenic plants</subject><subject>Water content</subject><subject>Water deficit</subject><subject>Water deprivation</subject><subject>water stress</subject><issn>0735-9640</issn><issn>1572-9818</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9UU2P0zAUjBBIlIUfwIkncW2Kn-N8cauWsF22EkjtigNCluvYXa9SJ9gOav_o_h4chQMnpPf05jAzT5pJkrdIVkhI-cEjIslTQqppWXp-liwwL2laV1g9TxakzPK0Lhh5mbzy_pFEDamqRfLU2AdhpWph33fKTRBCD99FUA4-KW2kCSBsCzvRGWvCBXbBKe_BWNhHuj8qayRsL9KMJzgIFyeC7Qq-dcIGD40M_WCk6LoLNOdh0hp7hPV-c4fL6AxrJw6m7QdvPISH-EVYARvjg2mNVXBnrPAKblTEPxrnRBhPH4ESJPBltGoJv1dAqyXYPt4lDCvIiuzn6-SFFp1Xb_7eq-T-c7O_3qTbrze31-ttKjNGQkoVImU5o6gJPbQllgedH4TWVSWlZqXMWVbIGHAd09W6zSqKZU1poWKslNbZVfJ-9h1c_2tUPvDHfnQ2vuRY5wUpcyxYZOHMkq733inNB2dOwl04Ej7Vx-f6eKxuWsbPUUNnjY9ce1TuH-f_iN7NIi16Lo7OeH6_i1llBIsCK0ayP4LIpLc</recordid><startdate>20090901</startdate><enddate>20090901</enddate><creator>Chen, Ni</creator><creator>Liu, Yan</creator><creator>Liu, Xin</creator><creator>Chai, Juan</creator><creator>Hu, Zhong</creator><creator>Liu, Heng</creator><general>New York : Springer-Verlag</general><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope></search><sort><creationdate>20090901</creationdate><title>Enhanced Tolerance to Water Deficit and Salinity Stress in Transgenic Lycium barbarum L. Plants Ectopically Expressing ATHK1, an Arabidopsis thaliana Histidine Kinase Gene [Erratum: 2010 June, v. 28, no. 2, p. 363]</title><author>Chen, Ni ; Liu, Yan ; Liu, Xin ; Chai, Juan ; Hu, Zhong ; Liu, Heng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c340t-2e11245421f02bd717bf5baff88ccf47c5436c1009110ffd382179226e9812293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Agrobacterium</topic><topic>Arabidopsis thaliana</topic><topic>Bioinformatics</topic><topic>Biomedical and Life Sciences</topic><topic>Catalase</topic><topic>Chlorophyll</topic><topic>Drought</topic><topic>drought tolerance</topic><topic>enzyme activity</topic><topic>genetic transformation</topic><topic>Histidine</topic><topic>Histidine kinase</topic><topic>Hydrogen peroxide</topic><topic>Kinases</topic><topic>Life Sciences</topic><topic>Lycium barbarum</topic><topic>Metabolomics</topic><topic>Moisture content</topic><topic>Osmolarity</topic><topic>Peroxidase</topic><topic>peroxidases</topic><topic>Plant Breeding/Biotechnology</topic><topic>Plant Sciences</topic><topic>plant stress</topic><topic>Polymerase chain reaction</topic><topic>Proline</topic><topic>Proteomics</topic><topic>reverse transcriptase polymerase chain reaction</topic><topic>Reverse transcription</topic><topic>salinity</topic><topic>salt stress</topic><topic>Salt tolerance</topic><topic>Sodium chloride</topic><topic>superoxide anion</topic><topic>Superoxide dismutase</topic><topic>Transgenic plants</topic><topic>Water content</topic><topic>Water deficit</topic><topic>Water deprivation</topic><topic>water stress</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Ni</creatorcontrib><creatorcontrib>Liu, Yan</creatorcontrib><creatorcontrib>Liu, Xin</creatorcontrib><creatorcontrib>Chai, Juan</creatorcontrib><creatorcontrib>Hu, Zhong</creatorcontrib><creatorcontrib>Liu, Heng</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>Plant molecular biology reporter</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Ni</au><au>Liu, Yan</au><au>Liu, Xin</au><au>Chai, Juan</au><au>Hu, Zhong</au><au>Liu, Heng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced Tolerance to Water Deficit and Salinity Stress in Transgenic Lycium barbarum L. Plants Ectopically Expressing ATHK1, an Arabidopsis thaliana Histidine Kinase Gene [Erratum: 2010 June, v. 28, no. 2, p. 363]</atitle><jtitle>Plant molecular biology reporter</jtitle><stitle>Plant Mol Biol Rep</stitle><date>2009-09-01</date><risdate>2009</risdate><volume>27</volume><issue>3</issue><spage>321</spage><epage>333</epage><pages>321-333</pages><issn>0735-9640</issn><eissn>1572-9818</eissn><abstract>ATHK1 has been implicated in drought and salt tolerance in Arabidopsis thaliana. In this study, the full-length coding sequence of ATHK1 was introduced into Lycium barbarum L. by Agrobacterium transformation. Our results indicated that the transgenic plants tolerated high concentrations of NaCl or water deprivation and exhibited faster recovery following re-watering compared to wild type plants. Salt- or water-stressed transgenic plants had higher relative water content, proline and soluble protein levels, and lower chlorophyll losses and membrane ion leakage. In addition, they showed higher capacity for antioxidative reactions reflected by reduced hydrogen peroxide (H₂O₂), superoxide anion radical (O₂ ⁻), and lipid peroxide production and increased superoxide dismutase, catalase, and peroxidase activities. The ATHK1 transcript, as shown by reverse transcription polymerase chain reaction, was more abundant under high than low osmolarity in transgenic plants. ATHK1 therefore improved tolerance of L. barbarum to drought and salt stress.</abstract><cop>New York</cop><pub>New York : Springer-Verlag</pub><doi>10.1007/s11105-008-0084-x</doi><tpages>13</tpages></addata></record> |
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| subjects | Agrobacterium Arabidopsis thaliana Bioinformatics Biomedical and Life Sciences Catalase Chlorophyll Drought drought tolerance enzyme activity genetic transformation Histidine Histidine kinase Hydrogen peroxide Kinases Life Sciences Lycium barbarum Metabolomics Moisture content Osmolarity Peroxidase peroxidases Plant Breeding/Biotechnology Plant Sciences plant stress Polymerase chain reaction Proline Proteomics reverse transcriptase polymerase chain reaction Reverse transcription salinity salt stress Salt tolerance Sodium chloride superoxide anion Superoxide dismutase Transgenic plants Water content Water deficit Water deprivation water stress |
| title | Enhanced Tolerance to Water Deficit and Salinity Stress in Transgenic Lycium barbarum L. Plants Ectopically Expressing ATHK1, an Arabidopsis thaliana Histidine Kinase Gene [Erratum: 2010 June, v. 28, no. 2, p. 363] |
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