Characterization of Five Putative Acyl Carrier Protein (ACP) Isoforms from Developing Seeds of Arachis hypogaea L
Five putative acyl carrier protein (ACP) cDNAs were isolated from developing peanut seeds by searching ESTs of a peanut immature-seed cDNA library and PCR-based cloning. Five peanut ACPs contained a strictly conserved Ser residue in the Asp-Ser-Leu (DSL) motif, which is an important characteristic o...
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| Veröffentlicht in: | Plant molecular biology reporter 2010-09, Vol.28 (3), p.365-372 |
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| creator | Li, Meng-Jun Wang, Xing-Jun Su, Lei Bi, Yu-Ping Wan, Shu-Bo |
| description | Five putative acyl carrier protein (ACP) cDNAs were isolated from developing peanut seeds by searching ESTs of a peanut immature-seed cDNA library and PCR-based cloning. Five peanut ACPs contained a strictly conserved Ser residue in the Asp-Ser-Leu (DSL) motif, which is an important characteristic of ACPs in plants and bacteria. Three AhACPs, AhACP1, AhACP4, AhACP5, were predicted to be located in chloroplast, while two AhACPs, AhACP2 and AhACP3, in mitochondria. Comparison of genomic DNA and cDNA sequences demonstrated three chloroplast ACPs (cpACPs) comprising of four exons and three introns while two mitochondrial ACPs (mtACPs) contained two exons and one intron. More than two homologs of each AhACP were expressed in developing peanut seeds. Most homologs were verified by corresponding genomic DNA sequences. Semi-quantitative RT-PCR analysis suggested AhACP1 was a seed-predominant ACP isoform. AhACP4 and AhACP5 showed same mRNA profile in different organs and during seed development. Two mtACPs expressed highly in peanut flower tissue which was distinct from three cpACPs. |
| doi_str_mv | 10.1007/s11105-009-0160-x |
| format | Article |
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Five peanut ACPs contained a strictly conserved Ser residue in the Asp-Ser-Leu (DSL) motif, which is an important characteristic of ACPs in plants and bacteria. Three AhACPs, AhACP1, AhACP4, AhACP5, were predicted to be located in chloroplast, while two AhACPs, AhACP2 and AhACP3, in mitochondria. Comparison of genomic DNA and cDNA sequences demonstrated three chloroplast ACPs (cpACPs) comprising of four exons and three introns while two mitochondrial ACPs (mtACPs) contained two exons and one intron. More than two homologs of each AhACP were expressed in developing peanut seeds. Most homologs were verified by corresponding genomic DNA sequences. Semi-quantitative RT-PCR analysis suggested AhACP1 was a seed-predominant ACP isoform. AhACP4 and AhACP5 showed same mRNA profile in different organs and during seed development. Two mtACPs expressed highly in peanut flower tissue which was distinct from three cpACPs.</description><identifier>ISSN: 0735-9640</identifier><identifier>EISSN: 1572-9818</identifier><identifier>DOI: 10.1007/s11105-009-0160-x</identifier><language>eng</language><publisher>New York: New York : Springer-Verlag</publisher><subject>Acyl carrier protein ; Arachis hypogaea ; Bacteria ; Bioinformatics ; Biomedical and Life Sciences ; chloroplasts ; Cloning ; conserved sequences ; Deoxyribonucleic acid ; DNA ; Exons ; gene expression ; Gene sequencing ; Homology ; Introns ; Isoforms ; Legumes ; Life Sciences ; messenger RNA ; Metabolomics ; Mitochondria ; Mitochondrial DNA ; molecular cloning ; mRNA ; Nucleotide sequence ; Organs ; Peanuts ; Plant Breeding/Biotechnology ; plant proteins ; Plant Sciences ; Polymerase chain reaction ; protein isoforms ; Proteomics ; reverse transcriptase polymerase chain reaction ; seed development ; Seeds ; sequence homology ; serine ; transport proteins</subject><ispartof>Plant molecular biology reporter, 2010-09, Vol.28 (3), p.365-372</ispartof><rights>Springer-Verlag 2009</rights><rights>Plant Molecular Biology Reporter is a copyright of Springer, 2010.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c340t-1eface0aaabd38cbd926751c2037f8dd90af52a1e0088517c8178f01f9cc19343</citedby><cites>FETCH-LOGICAL-c340t-1eface0aaabd38cbd926751c2037f8dd90af52a1e0088517c8178f01f9cc19343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11105-009-0160-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11105-009-0160-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Li, Meng-Jun</creatorcontrib><creatorcontrib>Wang, Xing-Jun</creatorcontrib><creatorcontrib>Su, Lei</creatorcontrib><creatorcontrib>Bi, Yu-Ping</creatorcontrib><creatorcontrib>Wan, Shu-Bo</creatorcontrib><title>Characterization of Five Putative Acyl Carrier Protein (ACP) Isoforms from Developing Seeds of Arachis hypogaea L</title><title>Plant molecular biology reporter</title><addtitle>Plant Mol Biol Rep</addtitle><description>Five putative acyl carrier protein (ACP) cDNAs were isolated from developing peanut seeds by searching ESTs of a peanut immature-seed cDNA library and PCR-based cloning. Five peanut ACPs contained a strictly conserved Ser residue in the Asp-Ser-Leu (DSL) motif, which is an important characteristic of ACPs in plants and bacteria. Three AhACPs, AhACP1, AhACP4, AhACP5, were predicted to be located in chloroplast, while two AhACPs, AhACP2 and AhACP3, in mitochondria. Comparison of genomic DNA and cDNA sequences demonstrated three chloroplast ACPs (cpACPs) comprising of four exons and three introns while two mitochondrial ACPs (mtACPs) contained two exons and one intron. More than two homologs of each AhACP were expressed in developing peanut seeds. Most homologs were verified by corresponding genomic DNA sequences. Semi-quantitative RT-PCR analysis suggested AhACP1 was a seed-predominant ACP isoform. AhACP4 and AhACP5 showed same mRNA profile in different organs and during seed development. Two mtACPs expressed highly in peanut flower tissue which was distinct from three cpACPs.</description><subject>Acyl carrier protein</subject><subject>Arachis hypogaea</subject><subject>Bacteria</subject><subject>Bioinformatics</subject><subject>Biomedical and Life Sciences</subject><subject>chloroplasts</subject><subject>Cloning</subject><subject>conserved sequences</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Exons</subject><subject>gene expression</subject><subject>Gene sequencing</subject><subject>Homology</subject><subject>Introns</subject><subject>Isoforms</subject><subject>Legumes</subject><subject>Life Sciences</subject><subject>messenger RNA</subject><subject>Metabolomics</subject><subject>Mitochondria</subject><subject>Mitochondrial DNA</subject><subject>molecular cloning</subject><subject>mRNA</subject><subject>Nucleotide sequence</subject><subject>Organs</subject><subject>Peanuts</subject><subject>Plant Breeding/Biotechnology</subject><subject>plant proteins</subject><subject>Plant Sciences</subject><subject>Polymerase chain reaction</subject><subject>protein isoforms</subject><subject>Proteomics</subject><subject>reverse transcriptase polymerase chain reaction</subject><subject>seed development</subject><subject>Seeds</subject><subject>sequence homology</subject><subject>serine</subject><subject>transport proteins</subject><issn>0735-9640</issn><issn>1572-9818</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kE1LAzEQhoMoWD9-gCcDXvSwOrPbNNljWa0WChbUc4jZpE1pNzXZltZfb8p68OQpQ3ifZ5iXkCuEewTgDxERgWUAZQY4gGx3RHrIeJ6VAsUx6QEvWFYO-nBKzmJcQGJAiB75quYqKN2a4L5V63xDvaUjtzV0umnTRxqGer-klQrBmUCnwbfGNfR2WE3v6Dh668MqUhv8ij6arVn6tWtm9M2YOh5UwySfu0jn-7WfKaPo5IKcWLWM5vL3PScfo6f36iWbvD6Pq-Ek00Uf2gyNVdqAUuqzLoT-rMt8wBnqHApuRV2XoCzLFRpIdzDkWiAXFtCWWmNZ9ItzctN518F_bUxs5cJvQpNWSizZABgXiCmFXUoHH2MwVq6DW6mwlwjy0KzsmpWpWXloVu4Sk3dMTNlmZsIf8z_QdQdZ5aWaBRflx1sOWACKnPE-FD8354Uf</recordid><startdate>20100901</startdate><enddate>20100901</enddate><creator>Li, Meng-Jun</creator><creator>Wang, Xing-Jun</creator><creator>Su, Lei</creator><creator>Bi, Yu-Ping</creator><creator>Wan, Shu-Bo</creator><general>New York : Springer-Verlag</general><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope></search><sort><creationdate>20100901</creationdate><title>Characterization of Five Putative Acyl Carrier Protein (ACP) Isoforms from Developing Seeds of Arachis hypogaea L</title><author>Li, Meng-Jun ; Wang, Xing-Jun ; Su, Lei ; Bi, Yu-Ping ; Wan, Shu-Bo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c340t-1eface0aaabd38cbd926751c2037f8dd90af52a1e0088517c8178f01f9cc19343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Acyl carrier protein</topic><topic>Arachis hypogaea</topic><topic>Bacteria</topic><topic>Bioinformatics</topic><topic>Biomedical and Life Sciences</topic><topic>chloroplasts</topic><topic>Cloning</topic><topic>conserved sequences</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Exons</topic><topic>gene expression</topic><topic>Gene sequencing</topic><topic>Homology</topic><topic>Introns</topic><topic>Isoforms</topic><topic>Legumes</topic><topic>Life Sciences</topic><topic>messenger RNA</topic><topic>Metabolomics</topic><topic>Mitochondria</topic><topic>Mitochondrial DNA</topic><topic>molecular cloning</topic><topic>mRNA</topic><topic>Nucleotide sequence</topic><topic>Organs</topic><topic>Peanuts</topic><topic>Plant Breeding/Biotechnology</topic><topic>plant proteins</topic><topic>Plant Sciences</topic><topic>Polymerase chain reaction</topic><topic>protein isoforms</topic><topic>Proteomics</topic><topic>reverse transcriptase polymerase chain reaction</topic><topic>seed development</topic><topic>Seeds</topic><topic>sequence homology</topic><topic>serine</topic><topic>transport proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Meng-Jun</creatorcontrib><creatorcontrib>Wang, Xing-Jun</creatorcontrib><creatorcontrib>Su, Lei</creatorcontrib><creatorcontrib>Bi, Yu-Ping</creatorcontrib><creatorcontrib>Wan, Shu-Bo</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>Plant molecular biology reporter</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Meng-Jun</au><au>Wang, Xing-Jun</au><au>Su, Lei</au><au>Bi, Yu-Ping</au><au>Wan, Shu-Bo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of Five Putative Acyl Carrier Protein (ACP) Isoforms from Developing Seeds of Arachis hypogaea L</atitle><jtitle>Plant molecular biology reporter</jtitle><stitle>Plant Mol Biol Rep</stitle><date>2010-09-01</date><risdate>2010</risdate><volume>28</volume><issue>3</issue><spage>365</spage><epage>372</epage><pages>365-372</pages><issn>0735-9640</issn><eissn>1572-9818</eissn><abstract>Five putative acyl carrier protein (ACP) cDNAs were isolated from developing peanut seeds by searching ESTs of a peanut immature-seed cDNA library and PCR-based cloning. Five peanut ACPs contained a strictly conserved Ser residue in the Asp-Ser-Leu (DSL) motif, which is an important characteristic of ACPs in plants and bacteria. Three AhACPs, AhACP1, AhACP4, AhACP5, were predicted to be located in chloroplast, while two AhACPs, AhACP2 and AhACP3, in mitochondria. Comparison of genomic DNA and cDNA sequences demonstrated three chloroplast ACPs (cpACPs) comprising of four exons and three introns while two mitochondrial ACPs (mtACPs) contained two exons and one intron. More than two homologs of each AhACP were expressed in developing peanut seeds. Most homologs were verified by corresponding genomic DNA sequences. Semi-quantitative RT-PCR analysis suggested AhACP1 was a seed-predominant ACP isoform. AhACP4 and AhACP5 showed same mRNA profile in different organs and during seed development. Two mtACPs expressed highly in peanut flower tissue which was distinct from three cpACPs.</abstract><cop>New York</cop><pub>New York : Springer-Verlag</pub><doi>10.1007/s11105-009-0160-x</doi><tpages>8</tpages></addata></record> |
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| subjects | Acyl carrier protein Arachis hypogaea Bacteria Bioinformatics Biomedical and Life Sciences chloroplasts Cloning conserved sequences Deoxyribonucleic acid DNA Exons gene expression Gene sequencing Homology Introns Isoforms Legumes Life Sciences messenger RNA Metabolomics Mitochondria Mitochondrial DNA molecular cloning mRNA Nucleotide sequence Organs Peanuts Plant Breeding/Biotechnology plant proteins Plant Sciences Polymerase chain reaction protein isoforms Proteomics reverse transcriptase polymerase chain reaction seed development Seeds sequence homology serine transport proteins |
| title | Characterization of Five Putative Acyl Carrier Protein (ACP) Isoforms from Developing Seeds of Arachis hypogaea L |
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